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The map label for this gene is nifS1 [H]

Identifier: 239618215

GI number: 239618215

Start: 1981718

End: 1982845

Strand: Reverse

Name: nifS1 [H]

Synonym: Kole_1851

Alternate gene names: 239618215

Gene position: 1982845-1981718 (Counterclockwise)

Preceding gene: 239618216

Following gene: 239618214

Centisome position: 86.13

GC content: 41.05

Gene sequence:

>1128_bases
ATGATATATCTTGATAACAACGCTACAACCAGGCTTGCTCCTGAAGTCCTTGAAACCATGACACGCTATTATATGCAAGA
TTACGCGAACCCCAATTCTATCCATTCCATGGGACTAACCGCCAGCATGGCTATGGAAGAAGCTAGAGAAAAAATAGCCA
ATCTTCTCGGTACAAACCCAAGAGAAATATTCTTCACTTCATGTGCAACTGAATCGATCAACTGGGCATTACGAACCGCT
ACCAGTTTCAGAGGAAATAGAAAAAAAGTTATTACAAGCTCCATAGAACACAAAGCGGTCTTAAACACCTTGAAAGATCT
CAAAAATACCCACAATATCGAAATCATTCAGGTACCCCCGGAAAGGAACGGTATCGTCCTTGCTGAAAAAATATTGAAAG
AAATCGATGACAATACTTACCTTGTCTCTTTGATGGCGGTTAATAATGTCACCGGAGCGATTCAACCCTTTGAAGAACTA
GGAAACCTTCTTGCGAAAAAAGGAATTTTTTATCATATAGACGCGGTACAAACTATAGGAAAAATACCTTTCAACTTAAA
AAAAGTGACATGCGATTTTGCAAGTTTCAGCGCTCATAAGTTTCACGGTCCGAAAGGTGTGGGCATCCTCTACGTTCGTA
AAAACACGAAGCTGCGTCCTTTCATCACCGGAGGCGGACAGGAAAGGGGCATGCGTTCTGGAACACAAAACGTTCCCGGA
ATAGTTGGAACAGCTTTAGCATTAGAATTAGCTGTCAATTCACTCGAAGAAACCTCTACCCGTCTCAATCAATACAGAAA
AATCATCATCAAAACAGTTAAAGAAATAGGCGGTGTAGTAAATACACCTTTCGAACATTCTATTCCTAACACTCTTAACA
TCTCTATTCCCGGAATAAGGGGAGAAATTCTGGTAAATGCTCTCTCCGATGAAGGAATTTGTGTGGGAACTTCTTCCGCT
TGCTCTTCACGAAGCGATGGTGGTCAATACGTCTTAGAAGCAATGAAGATCAATCCTGAACAGACCGCGTCGGCAATAAG
AATAAGTATGTCACGAGAAAATACTGAAAAGGAAATTCTGACCTTTGTCAATGTCCTAAAGAAACTGGCCGCCGCTTTGA
AATTTTGA

Upstream 100 bases:

>100_bases
GGAGAAAATCCTGGTTCAAAATTTCAAAAGGCCAAAGCCCTAAACGTCAAAACAATAAACGAAGAGGAATTCATTAAGTT
GATTCGGGAGGGAGAAAAAA

Downstream 100 bases:

>100_bases
CTCTAAACGGGTATAATTGTTCACAGTCAGATATAATTCTCTTTGAGGTGATCTAATGATTAGAAGTATGACAGGATTCG
CAAGAGCAGAGGAAGAAAAA

Product: Cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MIYLDNNATTRLAPEVLETMTRYYMQDYANPNSIHSMGLTASMAMEEAREKIANLLGTNPREIFFTSCATESINWALRTA
TSFRGNRKKVITSSIEHKAVLNTLKDLKNTHNIEIIQVPPERNGIVLAEKILKEIDDNTYLVSLMAVNNVTGAIQPFEEL
GNLLAKKGIFYHIDAVQTIGKIPFNLKKVTCDFASFSAHKFHGPKGVGILYVRKNTKLRPFITGGGQERGMRSGTQNVPG
IVGTALALELAVNSLEETSTRLNQYRKIIIKTVKEIGGVVNTPFEHSIPNTLNISIPGIRGEILVNALSDEGICVGTSSA
CSSRSDGGQYVLEAMKINPEQTASAIRISMSRENTEKEILTFVNVLKKLAAALKF

Sequences:

>Translated_375_residues
MIYLDNNATTRLAPEVLETMTRYYMQDYANPNSIHSMGLTASMAMEEAREKIANLLGTNPREIFFTSCATESINWALRTA
TSFRGNRKKVITSSIEHKAVLNTLKDLKNTHNIEIIQVPPERNGIVLAEKILKEIDDNTYLVSLMAVNNVTGAIQPFEEL
GNLLAKKGIFYHIDAVQTIGKIPFNLKKVTCDFASFSAHKFHGPKGVGILYVRKNTKLRPFITGGGQERGMRSGTQNVPG
IVGTALALELAVNSLEETSTRLNQYRKIIIKTVKEIGGVVNTPFEHSIPNTLNISIPGIRGEILVNALSDEGICVGTSSA
CSSRSDGGQYVLEAMKINPEQTASAIRISMSRENTEKEILTFVNVLKKLAAALKF
>Mature_375_residues
MIYLDNNATTRLAPEVLETMTRYYMQDYANPNSIHSMGLTASMAMEEAREKIANLLGTNPREIFFTSCATESINWALRTA
TSFRGNRKKVITSSIEHKAVLNTLKDLKNTHNIEIIQVPPERNGIVLAEKILKEIDDNTYLVSLMAVNNVTGAIQPFEEL
GNLLAKKGIFYHIDAVQTIGKIPFNLKKVTCDFASFSAHKFHGPKGVGILYVRKNTKLRPFITGGGQERGMRSGTQNVPG
IVGTALALELAVNSLEETSTRLNQYRKIIIKTVKEIGGVVNTPFEHSIPNTLNISIPGIRGEILVNALSDEGICVGTSSA
CSSRSDGGQYVLEAMKINPEQTASAIRISMSRENTEKEILTFVNVLKKLAAALKF

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=364, Percent_Identity=38.4615384615385, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=30.0970873786408, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI48994898, Length=380, Percent_Identity=41.3157894736842, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1787970, Length=304, Percent_Identity=26.6447368421053, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789175, Length=391, Percent_Identity=22.7621483375959, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=365, Percent_Identity=36.7123287671233, Blast_Score=241, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=28.1345565749235, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6319831, Length=379, Percent_Identity=35.8839050131926, Blast_Score=228, Evalue=9e-61,
Organism=Drosophila melanogaster, GI20129463, Length=371, Percent_Identity=37.1967654986523, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41246; Mature: 41246

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNNATTRLAPEVLETMTRYYMQDYANPNSIHSMGLTASMAMEEAREKIANLLGTNP
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCC
REIFFTSCATESINWALRTATSFRGNRKKVITSSIEHKAVLNTLKDLKNTHNIEIIQVPP
HHEEEEECCCCCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
ERNGIVLAEKILKEIDDNTYLVSLMAVNNVTGAIQPFEELGNLLAKKGIFYHIDAVQTIG
CCCCEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHH
KIPFNLKKVTCDFASFSAHKFHGPKGVGILYVRKNTKLRPFITGGGQERGMRSGTQNVPG
CCCCCEEEEEECHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCHHCCCCCCCCCCCC
IVGTALALELAVNSLEETSTRLNQYRKIIIKTVKEIGGVVNTPFEHSIPNTLNISIPGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
GEILVNALSDEGICVGTSSACSSRSDGGQYVLEAMKINPEQTASAIRISMSRENTEKEIL
CEEEEEEECCCCEEEECCHHCCCCCCCHHHHHHHHHCCHHHHHHHEEEEECCCCHHHHHH
TFVNVLKKLAAALKF
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYLDNNATTRLAPEVLETMTRYYMQDYANPNSIHSMGLTASMAMEEAREKIANLLGTNP
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCC
REIFFTSCATESINWALRTATSFRGNRKKVITSSIEHKAVLNTLKDLKNTHNIEIIQVPP
HHEEEEECCCCCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
ERNGIVLAEKILKEIDDNTYLVSLMAVNNVTGAIQPFEELGNLLAKKGIFYHIDAVQTIG
CCCCEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHH
KIPFNLKKVTCDFASFSAHKFHGPKGVGILYVRKNTKLRPFITGGGQERGMRSGTQNVPG
CCCCCEEEEEECHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCHHCCCCCCCCCCCC
IVGTALALELAVNSLEETSTRLNQYRKIIIKTVKEIGGVVNTPFEHSIPNTLNISIPGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
GEILVNALSDEGICVGTSSACSSRSDGGQYVLEAMKINPEQTASAIRISMSRENTEKEIL
CEEEEEEECCCCEEEECCHHCCCCCCCHHHHHHHHHCCHHHHHHHEEEEECCCCHHHHHH
TFVNVLKKLAAALKF
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]