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The map label for this gene is ligA [H]

Identifier: 239618216

GI number: 239618216

Start: 1982832

End: 1984850

Strand: Reverse

Name: ligA [H]

Synonym: Kole_1852

Alternate gene names: 239618216

Gene position: 1984850-1982832 (Counterclockwise)

Preceding gene: 239618217

Following gene: 239618215

Centisome position: 86.22

GC content: 41.65

Gene sequence:

>2019_bases
ATGAGAGCCCCCCATGATGTAATTGAACGGGCAAAAAAATTGAGAGAAGAACTGAATTACCACGCCTATAGATATTACGT
ACTGAACGATCCAATAATCACCGACGAAGAATATGACAGGATGTTAAAGGAGCTCCAGGAACTTGAAAAAAAATACCCGG
AGCTAATTACTCCTGACTCTCCAACCCAAAGGATTGGGGCTAAGCCCCTAAATGGCTTTGAACAGGTCATTCATTCTTCC
AGGCTTTACAGCCTGGACAATACTTATTCTGAAGAAGAACTCAAAGGCTTCGATAGAAGAATAAAAAAATTACTCAATGT
CGAACAGCTCGATTATGTGTGCGAACTAAAAATAGATGGGCTTTCCGTTACCCTAAGGTATGAAGAAGGAATTCTCGTTT
TGGGAGCTACCCGGGGCGATGGTACCGTTGGAGAAAATGTCACAAAAAATATCAGAACGATAAAGTCAATACCCTTGAGA
CTAAGAAAACCACTTACGATAGAAATAAGAGGAGAAGTTTATCTCCCCAAAAGCGAATTCAAAAAAATAAATGACGAAAG
GGCCGAACAGGAATTGCCTCTCTTTGCCAATCCGCGAAATGCAGCCGCTGGAACGCTAAGACAACTTGATCCAAAAGAAG
TAGCCAAAAGAAATCTCGATGCCTTTTTCTATCAGATAGTTTCCCCGGAGAACTATGAATTAGAAACTCAATGGGAGGTT
CTGCAGTTTCTGAAAGAAATTGGTATGAAAACTGAACCACATGCCAGGCTGGTCAGCGATATCTCAGAGGTTATCGAGTT
CTGGAAAGAATGGTCCGAATCGAGAAATAACTTAGACTATGCAATAGATGGACTCGTTGTTAAAGTAAACAGGATAGAAT
TTCAAAATAAGCTTGGTTACACCGCCAAAAGTCCACGCTGGGCAATCGCTTTCAAATTTCCAGCCGAGCAAGCACGAACA
AAACTCCTCGATGTTACCTTTCAGGTTGGAAGAACAGGTGTAATAACCCCTGTAGCTGAACTGGAACCGGTTAAACTTGC
CGGAACAATTGTTAAACGTGCTACTTTACATAATTTTGACTACATTCGAGATAGGGATATAAGAATCGGTGACACTGTAA
TAGTAGAAAAAGCTGGAGAAATAATCCCGCAAATCGTTAAACCAATAGCCGAAACCCGATCGGGCAAAGAAAAAGTCATT
GAACCTCCATCAAGCTGTCCAGTTTGCGGTGGAAAGGTTGGGAAAGCAAAAGAAGACGAAGTAGCTTTACGCTGCCTCAA
CCCTGCTTGTCCTGCTAAGACGGAAAGGAGGATTTTGCTGTTCGTTTCACGTAATGCTATGGACATAAATGGACTCGGAG
AGAAAATGATAAAAAGGCTCGTTCATTCGGGACTTGTAAAAAGCCCGGCTGACCTCTATAAACTCACCCCTTTTGATCTT
GCGCAACTCGGCAACGGAATAGGAGAAAAAACCATAAGGAACTTCTATAAAGAACTTGAAAAAAGTAGAAAAAGACCTTT
GCACAAGCTTCTTGCCGGGCTTGGCATTCCAGGTGTCGGAGTTAAACTCGCAAGGGATCTGGCGGAACATTTTAAAACCC
TTGATGCACTTCAAAATGCAACAATCGACCAGCTTCTTGAAGTTCCGGGCATCGGACTGGAATTGGCAAAAAACATAGTG
GAATTTTTCAACCTTCCAGAGATAAAGGAAGAAATTGAGCACCTCAAGAAAGAAGTTAATACCTCAGAACGTTCCTCGAA
AAAAGAAGAGCTTCTCAAAGGGAAAAAATTTGTGTTGACCGGAACCTTGAAGAACTTCACCAGAAAAGAAGCGGAAGAAC
TCATAGTCTCTCTGGGAGGAAAAGTGAGTTCCAGCGTCTCAAAAAATACCGATTTCGTGGTCGTTGGAGAAAATCCTGGT
TCAAAATTTCAAAAGGCCAAAGCCCTAAACGTCAAAACAATAAACGAAGAGGAATTCATTAAGTTGATTCGGGAGGGAGA
AAAAAATGATATATCTTGA

Upstream 100 bases:

>100_bases
AACTCAAAAAACTACTTAATGATATTGAATTACAGGTCAGCATATCCCATGAAAAAGAGTATGCCATTGCGGTTGTAATT
GTACTGAGGAGGGATGAAAA

Downstream 100 bases:

>100_bases
TAACAACGCTACAACCAGGCTTGCTCCTGAAGTCCTTGAAACCATGACACGCTATTATATGCAAGATTACGCGAACCCCA
ATTCTATCCATTCCATGGGA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MRAPHDVIERAKKLREELNYHAYRYYVLNDPIITDEEYDRMLKELQELEKKYPELITPDSPTQRIGAKPLNGFEQVIHSS
RLYSLDNTYSEEELKGFDRRIKKLLNVEQLDYVCELKIDGLSVTLRYEEGILVLGATRGDGTVGENVTKNIRTIKSIPLR
LRKPLTIEIRGEVYLPKSEFKKINDERAEQELPLFANPRNAAAGTLRQLDPKEVAKRNLDAFFYQIVSPENYELETQWEV
LQFLKEIGMKTEPHARLVSDISEVIEFWKEWSESRNNLDYAIDGLVVKVNRIEFQNKLGYTAKSPRWAIAFKFPAEQART
KLLDVTFQVGRTGVITPVAELEPVKLAGTIVKRATLHNFDYIRDRDIRIGDTVIVEKAGEIIPQIVKPIAETRSGKEKVI
EPPSSCPVCGGKVGKAKEDEVALRCLNPACPAKTERRILLFVSRNAMDINGLGEKMIKRLVHSGLVKSPADLYKLTPFDL
AQLGNGIGEKTIRNFYKELEKSRKRPLHKLLAGLGIPGVGVKLARDLAEHFKTLDALQNATIDQLLEVPGIGLELAKNIV
EFFNLPEIKEEIEHLKKEVNTSERSSKKEELLKGKKFVLTGTLKNFTRKEAEELIVSLGGKVSSSVSKNTDFVVVGENPG
SKFQKAKALNVKTINEEEFIKLIREGEKNDIS

Sequences:

>Translated_672_residues
MRAPHDVIERAKKLREELNYHAYRYYVLNDPIITDEEYDRMLKELQELEKKYPELITPDSPTQRIGAKPLNGFEQVIHSS
RLYSLDNTYSEEELKGFDRRIKKLLNVEQLDYVCELKIDGLSVTLRYEEGILVLGATRGDGTVGENVTKNIRTIKSIPLR
LRKPLTIEIRGEVYLPKSEFKKINDERAEQELPLFANPRNAAAGTLRQLDPKEVAKRNLDAFFYQIVSPENYELETQWEV
LQFLKEIGMKTEPHARLVSDISEVIEFWKEWSESRNNLDYAIDGLVVKVNRIEFQNKLGYTAKSPRWAIAFKFPAEQART
KLLDVTFQVGRTGVITPVAELEPVKLAGTIVKRATLHNFDYIRDRDIRIGDTVIVEKAGEIIPQIVKPIAETRSGKEKVI
EPPSSCPVCGGKVGKAKEDEVALRCLNPACPAKTERRILLFVSRNAMDINGLGEKMIKRLVHSGLVKSPADLYKLTPFDL
AQLGNGIGEKTIRNFYKELEKSRKRPLHKLLAGLGIPGVGVKLARDLAEHFKTLDALQNATIDQLLEVPGIGLELAKNIV
EFFNLPEIKEEIEHLKKEVNTSERSSKKEELLKGKKFVLTGTLKNFTRKEAEELIVSLGGKVSSSVSKNTDFVVVGENPG
SKFQKAKALNVKTINEEEFIKLIREGEKNDIS
>Mature_672_residues
MRAPHDVIERAKKLREELNYHAYRYYVLNDPIITDEEYDRMLKELQELEKKYPELITPDSPTQRIGAKPLNGFEQVIHSS
RLYSLDNTYSEEELKGFDRRIKKLLNVEQLDYVCELKIDGLSVTLRYEEGILVLGATRGDGTVGENVTKNIRTIKSIPLR
LRKPLTIEIRGEVYLPKSEFKKINDERAEQELPLFANPRNAAAGTLRQLDPKEVAKRNLDAFFYQIVSPENYELETQWEV
LQFLKEIGMKTEPHARLVSDISEVIEFWKEWSESRNNLDYAIDGLVVKVNRIEFQNKLGYTAKSPRWAIAFKFPAEQART
KLLDVTFQVGRTGVITPVAELEPVKLAGTIVKRATLHNFDYIRDRDIRIGDTVIVEKAGEIIPQIVKPIAETRSGKEKVI
EPPSSCPVCGGKVGKAKEDEVALRCLNPACPAKTERRILLFVSRNAMDINGLGEKMIKRLVHSGLVKSPADLYKLTPFDL
AQLGNGIGEKTIRNFYKELEKSRKRPLHKLLAGLGIPGVGVKLARDLAEHFKTLDALQNATIDQLLEVPGIGLELAKNIV
EFFNLPEIKEEIEHLKKEVNTSERSSKKEELLKGKKFVLTGTLKNFTRKEAEELIVSLGGKVSSSVSKNTDFVVVGENPG
SKFQKAKALNVKTINEEEFIKLIREGEKNDIS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=666, Percent_Identity=44.2942942942943, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI87082305, Length=570, Percent_Identity=22.280701754386, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 76198; Mature: 76198

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPHDVIERAKKLREELNYHAYRYYVLNDPIITDEEYDRMLKELQELEKKYPELITPDS
CCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PTQRIGAKPLNGFEQVIHSSRLYSLDNTYSEEELKGFDRRIKKLLNVEQLDYVCELKIDG
CHHHCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCHHHHCEEEEEEECC
LSVTLRYEEGILVLGATRGDGTVGENVTKNIRTIKSIPLRLRKPLTIEIRGEVYLPKSEF
EEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEEECCCEECCHHHH
KKINDERAEQELPLFANPRNAAAGTLRQLDPKEVAKRNLDAFFYQIVSPENYELETQWEV
HHCCHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LQFLKEIGMKTEPHARLVSDISEVIEFWKEWSESRNNLDYAIDGLVVKVNRIEFQNKLGY
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEHHHHHHHCCC
TAKSPRWAIAFKFPAEQARTKLLDVTFQVGRTGVITPVAELEPVKLAGTIVKRATLHNFD
CCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCC
YIRDRDIRIGDTVIVEKAGEIIPQIVKPIAETRSGKEKVIEPPSSCPVCGGKVGKAKEDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCHH
VALRCLNPACPAKTERRILLFVSRNAMDINGLGEKMIKRLVHSGLVKSPADLYKLTPFDL
HHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCHHH
AQLGNGIGEKTIRNFYKELEKSRKRPLHKLLAGLGIPGVGVKLARDLAEHFKTLDALQNA
HHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
TIDQLLEVPGIGLELAKNIVEFFNLPEIKEEIEHLKKEVNTSERSSKKEELLKGKKFVLT
CHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEE
GTLKNFTRKEAEELIVSLGGKVSSSVSKNTDFVVVGENPGSKFQKAKALNVKTINEEEFI
ECHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCHHHHHHCCCEEECCHHHHH
KLIREGEKNDIS
HHHHCCCCCCCC
>Mature Secondary Structure
MRAPHDVIERAKKLREELNYHAYRYYVLNDPIITDEEYDRMLKELQELEKKYPELITPDS
CCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PTQRIGAKPLNGFEQVIHSSRLYSLDNTYSEEELKGFDRRIKKLLNVEQLDYVCELKIDG
CHHHCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCHHHHCEEEEEEECC
LSVTLRYEEGILVLGATRGDGTVGENVTKNIRTIKSIPLRLRKPLTIEIRGEVYLPKSEF
EEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEEECCCEECCHHHH
KKINDERAEQELPLFANPRNAAAGTLRQLDPKEVAKRNLDAFFYQIVSPENYELETQWEV
HHCCHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LQFLKEIGMKTEPHARLVSDISEVIEFWKEWSESRNNLDYAIDGLVVKVNRIEFQNKLGY
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEHHHHHHHCCC
TAKSPRWAIAFKFPAEQARTKLLDVTFQVGRTGVITPVAELEPVKLAGTIVKRATLHNFD
CCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCC
YIRDRDIRIGDTVIVEKAGEIIPQIVKPIAETRSGKEKVIEPPSSCPVCGGKVGKAKEDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCHH
VALRCLNPACPAKTERRILLFVSRNAMDINGLGEKMIKRLVHSGLVKSPADLYKLTPFDL
HHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCHHH
AQLGNGIGEKTIRNFYKELEKSRKRPLHKLLAGLGIPGVGVKLARDLAEHFKTLDALQNA
HHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
TIDQLLEVPGIGLELAKNIVEFFNLPEIKEEIEHLKKEVNTSERSSKKEELLKGKKFVLT
CHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEE
GTLKNFTRKEAEELIVSLGGKVSSSVSKNTDFVVVGENPGSKFQKAKALNVKTINEEEFI
ECHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCHHHHHHCCCEEECCHHHHH
KLIREGEKNDIS
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA