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The map label for this gene is rlmH

Identifier: 239618086

GI number: 239618086

Start: 1847382

End: 1847849

Strand: Reverse

Name: rlmH

Synonym: Kole_1718

Alternate gene names: 239618086

Gene position: 1847849-1847382 (Counterclockwise)

Preceding gene: 239618087

Following gene: 239618085

Centisome position: 80.27

GC content: 39.53

Gene sequence:

>468_bases
ATGAATCTTGAAATCTATCTAACGGGTAAAGTGAAAACCAAATTCATTTTAGAAGGTGTGGAACAATATCTAAAATGGAT
TAGACCATATCATAAAATTAAAATAACGTCTTTCCCTCTAGCCGGCAGCACATCGGCCAACAGGGATCAAATAAAGAAAA
AAGAAGGCGAACGCTACCTCAAAGCGTTGCAAAATGAAAAGAACGTTGTTGTGCTTCACGAGCGCGGTGAAGAACTGAGC
TCTATGGAATTTGCAACTTTTATAAAAAAGTGGCAAAATAGTGGTACGAGGAAGCTCATATTCATAATCGGAGGGCCTCT
GGGGTTCAGTGATAACGTGCTTTCACAAAACTGGAAGAAATTATCGCTGTCACGAATGACCTTCACCCACGAAATGGCCC
TTCTTGTCTTACTTGAACAGCTGTACAGAGCTGAAACTATAAACCGTGGAATGATTTATCATTATTGA

Upstream 100 bases:

>100_bases
GACATTGTTTTATTGGGACTAAATTCCTATACTGAAATCCCTTACATGTTTTCACGAATTACTGTTGTTTCTGTAATAAA
ATCAGGCAGAGTGGTTTTCC

Downstream 100 bases:

>100_bases
AAAACAACCGATATTTTTTTGGAGGTGGAACAAGAGTGGAAGAACCTGCCAGTAGTGGTGATCCTTTATCTATTATCATT
AATCTCATTTTTTTGATTTT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MNLEIYLTGKVKTKFILEGVEQYLKWIRPYHKIKITSFPLAGSTSANRDQIKKKEGERYLKALQNEKNVVVLHERGEELS
SMEFATFIKKWQNSGTRKLIFIIGGPLGFSDNVLSQNWKKLSLSRMTFTHEMALLVLLEQLYRAETINRGMIYHY

Sequences:

>Translated_155_residues
MNLEIYLTGKVKTKFILEGVEQYLKWIRPYHKIKITSFPLAGSTSANRDQIKKKEGERYLKALQNEKNVVVLHERGEELS
SMEFATFIKKWQNSGTRKLIFIIGGPLGFSDNVLSQNWKKLSLSRMTFTHEMALLVLLEQLYRAETINRGMIYHY
>Mature_155_residues
MNLEIYLTGKVKTKFILEGVEQYLKWIRPYHKIKITSFPLAGSTSANRDQIKKKEGERYLKALQNEKNVVVLHERGEELS
SMEFATFIKKWQNSGTRKLIFIIGGPLGFSDNVLSQNWKKLSLSRMTFTHEMALLVLLEQLYRAETINRGMIYHY

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=28.2051282051282, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_KOSOT (C5CFQ6)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941408.1
- ProteinModelPortal:   C5CFQ6
- GeneID:   7968119
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1718
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18115; Mature: 18115

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEIYLTGKVKTKFILEGVEQYLKWIRPYHKIKITSFPLAGSTSANRDQIKKKEGERYL
CCEEEEEECCCHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCHHHHHHHHHHHHH
KALQNEKNVVVLHERGEELSSMEFATFIKKWQNSGTRKLIFIIGGPLGFSDNVLSQNWKK
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHCCHHH
LSLSRMTFTHEMALLVLLEQLYRAETINRGMIYHY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MNLEIYLTGKVKTKFILEGVEQYLKWIRPYHKIKITSFPLAGSTSANRDQIKKKEGERYL
CCEEEEEECCCHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCHHHHHHHHHHHHH
KALQNEKNVVVLHERGEELSSMEFATFIKKWQNSGTRKLIFIIGGPLGFSDNVLSQNWKK
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHCCHHH
LSLSRMTFTHEMALLVLLEQLYRAETINRGMIYHY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA