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The map label for this gene is cysS [H]

Identifier: 239618081

GI number: 239618081

Start: 1842260

End: 1843636

Strand: Reverse

Name: cysS [H]

Synonym: Kole_1713

Alternate gene names: 239618081

Gene position: 1843636-1842260 (Counterclockwise)

Preceding gene: 239618082

Following gene: 239618079

Centisome position: 80.08

GC content: 45.9

Gene sequence:

>1377_bases
GTGCTGAGGATAACAAACACTCTCACAGGAAAAAAGGAAGAATTCATCCCGATAACCCCCGGAGAGGTCAGGATCTACGT
TTGCGGACCCACGGTGTACAATTTTGTGCATCTCGGGAACAGCCGCCCCGCCATAACCTTCGATGCCTTCAGGAGATATC
TCGAGTACCGGGGATACCGGGTAATTCTTGCTCAAAACTTCACCGATATTGATGACAAAATAATCATCAAAGCGAATGAG
GAAGGTGTGGATGCGCTGGAAATCGCTGAGCGTTACATCGTTCAGTACTGGAAGGATTCTCTGGCTCTGGGGATAAGGCC
GGCAAACTTCCACCCGAGAACGACTCATTTCGTAGATGAAATTATAGAATTCATACAGGATCTGATCGATAAGGGTTTTG
CCTACGTTGTAGACGGCGACGTGTACTTTAATGTCTCGGAATTCAAAAATTATGGAGAACTATCTCACAGGAAACTCGAA
GATATGATAGCAGGTGCGAGAATCGAGCCGGGTGAAAAGAAGAAATCCCCGGAAGATTTCGCATTGTGGAAAGCCGCAAA
AGAAGGGGAACCTTTTTGGAACAGCCCCTGGGGACCCGGAAGGCCCGGCTGGCATATAGAGTGCTCGGTGATGTCAACAT
GCATCCTCGGCGAGACCTTTGACATCCATGCTGGTGGAGCTGATCTGATATTCCCGCACCATGAGAATGAAAAAGCTCAA
TCAGAAGCGCGACATGGCAAGACCTTCGCCAGGTACTGGATGCATAACGGCATGATGAGACTCGCGGGCTCCAAGATGTC
AAAATCTCTCGGAAATTTCATCACGATAAAAGATGCCGTCAAAAAATATGGAAAGGACGCAATCAGATTGTTTATCTTCT
CCAAGCACTACCGTTCGCCGATAGACTACTCTGAAGAGATGCTCGCTGAAAACCTGAGAGCGGTGAAAAGGGTAAACGCT
ATACTCCGGGATTTCGAAAAATCCGAAGGTGACAAACCACTGTTTTCAAAAAAGACGCCTTTCGTGAAGGAACAAATAGA
ACGTTTTATCGAAGCACTGGATGACGACTTTAACACCCCAAAAGCCGTTGCCTTGATGTTCGATCTGATCAATAAAATGG
GTGAGACAAAAAACAGAAACAGAAAACTGGAAATCTATCATCTTATCCGAAATGAATTCGGCCCCGTTCTGGGAATACTC
GAAAAGGACGAAGAGGTAAGTGCAAACATCGATGGTCTCGTAAAACTACTGATAGAGATACGCGCAGAATTCAAGAAGGA
TAAAAACTACGAGAAAGCTGATCAGATCCGAGATAGGCTCAACGAACTTGGAATAAAACTCATGGATACACCGGAAGGAA
CGAAGTACACTTTCTAA

Upstream 100 bases:

>100_bases
TTCTCACAGGTATGGACGAAGGGCCTGAACTCGTTGAAATTCTCTACCTGCTTGGAAGAAATAAAGTGATAGACAGAATA
GATAGGGCGGTGGGTAATCT

Downstream 100 bases:

>100_bases
AGTTCTGGCGTTGCTGACGCTTGACAACTTCAATTGTCGATGTTAGAATTTACATCGTTGGAGGGTGCGTAGCTCAGTGG
GAGAGCGCTTCCTTGACGAG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MLRITNTLTGKKEEFIPITPGEVRIYVCGPTVYNFVHLGNSRPAITFDAFRRYLEYRGYRVILAQNFTDIDDKIIIKANE
EGVDALEIAERYIVQYWKDSLALGIRPANFHPRTTHFVDEIIEFIQDLIDKGFAYVVDGDVYFNVSEFKNYGELSHRKLE
DMIAGARIEPGEKKKSPEDFALWKAAKEGEPFWNSPWGPGRPGWHIECSVMSTCILGETFDIHAGGADLIFPHHENEKAQ
SEARHGKTFARYWMHNGMMRLAGSKMSKSLGNFITIKDAVKKYGKDAIRLFIFSKHYRSPIDYSEEMLAENLRAVKRVNA
ILRDFEKSEGDKPLFSKKTPFVKEQIERFIEALDDDFNTPKAVALMFDLINKMGETKNRNRKLEIYHLIRNEFGPVLGIL
EKDEEVSANIDGLVKLLIEIRAEFKKDKNYEKADQIRDRLNELGIKLMDTPEGTKYTF

Sequences:

>Translated_458_residues
MLRITNTLTGKKEEFIPITPGEVRIYVCGPTVYNFVHLGNSRPAITFDAFRRYLEYRGYRVILAQNFTDIDDKIIIKANE
EGVDALEIAERYIVQYWKDSLALGIRPANFHPRTTHFVDEIIEFIQDLIDKGFAYVVDGDVYFNVSEFKNYGELSHRKLE
DMIAGARIEPGEKKKSPEDFALWKAAKEGEPFWNSPWGPGRPGWHIECSVMSTCILGETFDIHAGGADLIFPHHENEKAQ
SEARHGKTFARYWMHNGMMRLAGSKMSKSLGNFITIKDAVKKYGKDAIRLFIFSKHYRSPIDYSEEMLAENLRAVKRVNA
ILRDFEKSEGDKPLFSKKTPFVKEQIERFIEALDDDFNTPKAVALMFDLINKMGETKNRNRKLEIYHLIRNEFGPVLGIL
EKDEEVSANIDGLVKLLIEIRAEFKKDKNYEKADQIRDRLNELGIKLMDTPEGTKYTF
>Mature_458_residues
MLRITNTLTGKKEEFIPITPGEVRIYVCGPTVYNFVHLGNSRPAITFDAFRRYLEYRGYRVILAQNFTDIDDKIIIKANE
EGVDALEIAERYIVQYWKDSLALGIRPANFHPRTTHFVDEIIEFIQDLIDKGFAYVVDGDVYFNVSEFKNYGELSHRKLE
DMIAGARIEPGEKKKSPEDFALWKAAKEGEPFWNSPWGPGRPGWHIECSVMSTCILGETFDIHAGGADLIFPHHENEKAQ
SEARHGKTFARYWMHNGMMRLAGSKMSKSLGNFITIKDAVKKYGKDAIRLFIFSKHYRSPIDYSEEMLAENLRAVKRVNA
ILRDFEKSEGDKPLFSKKTPFVKEQIERFIEALDDDFNTPKAVALMFDLINKMGETKNRNRKLEIYHLIRNEFGPVLGIL
EKDEEVSANIDGLVKLLIEIRAEFKKDKNYEKADQIRDRLNELGIKLMDTPEGTKYTF

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=382, Percent_Identity=39.2670157068063, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI62240992, Length=417, Percent_Identity=34.052757793765, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI303304987, Length=417, Percent_Identity=34.052757793765, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI21269877, Length=417, Percent_Identity=34.052757793765, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI10835051, Length=417, Percent_Identity=34.052757793765, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1786737, Length=469, Percent_Identity=46.2686567164179, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17509795, Length=437, Percent_Identity=32.9519450800915, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17509797, Length=437, Percent_Identity=32.9519450800915, Blast_Score=205, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6324082, Length=311, Percent_Identity=39.871382636656, Blast_Score=216, Evalue=8e-57,
Organism=Drosophila melanogaster, GI19922218, Length=509, Percent_Identity=33.0058939096267, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI19922364, Length=233, Percent_Identity=43.7768240343348, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 52845; Mature: 52845

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRITNTLTGKKEEFIPITPGEVRIYVCGPTVYNFVHLGNSRPAITFDAFRRYLEYRGYR
CEEECCCCCCCCCCEECCCCCCEEEEEECCHHHHHEEECCCCCCEEHHHHHHHHHHCCEE
VILAQNFTDIDDKIIIKANEEGVDALEIAERYIVQYWKDSLALGIRPANFHPRTTHFVDE
EEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHH
IIEFIQDLIDKGFAYVVDGDVYFNVSEFKNYGELSHRKLEDMIAGARIEPGEKKKSPEDF
HHHHHHHHHHCCCEEEECCCEEEEHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
ALWKAAKEGEPFWNSPWGPGRPGWHIECSVMSTCILGETFDIHAGGADLIFPHHENEKAQ
HHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHH
SEARHGKTFARYWMHNGMMRLAGSKMSKSLGNFITIKDAVKKYGKDAIRLFIFSKHYRSP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEHHHHHHHHCCHHEEEEEEHHHCCCC
IDYSEEMLAENLRAVKRVNAILRDFEKSEGDKPLFSKKTPFVKEQIERFIEALDDDFNTP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
KAVALMFDLINKMGETKNRNRKLEIYHLIRNEFGPVLGILEKDEEVSANIDGLVKLLIEI
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCEEHHCCCCHHHCCHHHHHHHHHHH
RAEFKKDKNYEKADQIRDRLNELGIKLMDTPEGTKYTF
HHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCC
>Mature Secondary Structure
MLRITNTLTGKKEEFIPITPGEVRIYVCGPTVYNFVHLGNSRPAITFDAFRRYLEYRGYR
CEEECCCCCCCCCCEECCCCCCEEEEEECCHHHHHEEECCCCCCEEHHHHHHHHHHCCEE
VILAQNFTDIDDKIIIKANEEGVDALEIAERYIVQYWKDSLALGIRPANFHPRTTHFVDE
EEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHH
IIEFIQDLIDKGFAYVVDGDVYFNVSEFKNYGELSHRKLEDMIAGARIEPGEKKKSPEDF
HHHHHHHHHHCCCEEEECCCEEEEHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
ALWKAAKEGEPFWNSPWGPGRPGWHIECSVMSTCILGETFDIHAGGADLIFPHHENEKAQ
HHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHH
SEARHGKTFARYWMHNGMMRLAGSKMSKSLGNFITIKDAVKKYGKDAIRLFIFSKHYRSP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEHHHHHHHHCCHHEEEEEEHHHCCCC
IDYSEEMLAENLRAVKRVNAILRDFEKSEGDKPLFSKKTPFVKEQIERFIEALDDDFNTP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
KAVALMFDLINKMGETKNRNRKLEIYHLIRNEFGPVLGILEKDEEVSANIDGLVKLLIEI
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCEEHHCCCCHHHCCHHHHHHHHHHH
RAEFKKDKNYEKADQIRDRLNELGIKLMDTPEGTKYTF
HHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]