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The map label for this gene is proS

Identifier: 239618079

GI number: 239618079

Start: 1839337

End: 1841082

Strand: Reverse

Name: proS

Synonym: Kole_1711

Alternate gene names: 239618079

Gene position: 1841082-1839337 (Counterclockwise)

Preceding gene: 239618081

Following gene: 239618078

Centisome position: 79.97

GC content: 43.99

Gene sequence:

>1746_bases
ATGCGTTTTTCACAGTTGTATGCCCCTACACTAAAAGAAGCACCTTCCGATGCCGATCTTGTAAGTATCAAACTTTTAAT
CCGGGGAGGATTTGTAAGGAAAAATGCAGCTGGAGTCTATACATATCTGCCGCTCGGACTCAGGGTATTAAAAAAAGTGG
AGCAAATCGTCCGTGAAGAAATGGCAGCTATCGGTTGCCAGGAGATTCTCATGCCCATCATTCAGCCTGCGGAGCTGTGG
TTCGAATCCGGAAGATGGGACGATTATGGTCCAGAAATGATGAAATTCAAGGATCGTCATGAACGTGATTTTACTCTCGG
TCCAACACATGAAGAGTTACTGACAAGCATAGTAAGGAATGAATTAAGATCTTATAGACAGTTTCCTCTTTCTCTATTTC
AAATAGCCAACAAATATAGGGATGAAATAAGGCCACGCTTTGGTTTGATAAGGGCACGAGAATTTCTCATGAAAGATGCA
TACAGTTTTCACACAGACTGGGAGTCTTTAGATAAAGCCTATAAAGATTTCTATAAAGCATATGGCAGAATCATGGAAAG
AATAGGTCTTAAATATCTCGTCGTTGAAGCGGATACTGGCGCAATAGGCGGAGATGAATCACATGAATTCAACGCCCTAG
CAGACACAGGCGAGTCAACGTTGCTTTACTGTGATTGTGGCTATGCGGCAAGTGACGAAAAGGCTGAATACATGATGCTC
AGCGACGAAGATCCTGATGTTCAGGAAGAAAAGGCACTTGAACTGGTTGAAACACCCGGCGTCAGAACCGTACAGGAAGT
TGCGGATTTCCTGAAAGTAACACCGGAGCAGATTGTAAAGTCCCTGCTGTACAGGGGCAAAGAAGGGTTCGTAATGGCTC
TTATAAGAGGCGATCAGGAACTGAACGAATCCAAGCTTAAGGCACATTTGAAGGACCAGACCCTGACAATGGCAACTCTG
GAAGAAGTTCTTGAAAACTTCGGCGTCCCGATAGGATTCATAGGGCCTGTGGGTATGAACGACAAGGTCAAAATCGTAGC
CGATTTCACAGTAAAACCATTGAGAAATTTCGTTGTTGGTGGAATGAAGGAAGGCTATCACTATAAGGGCGTCTGTTTAG
GTAGAGATTTTTCTGTTGATGAATGGTTCGATCTCAAACTAGCCGTTGAAGGCGATCCCTGTCCAAAGTGCGGAAAACCA
ATGAAGATGACCAAAGGTATAGAGCTAGGGCATATCTTCAAGCTCGGGACCAAATACTCCGAAAAGATGAACGGATATTT
CACAGATGAAAATGGCGAAAATCATCCTTATATAATGGGATGTTATGGTTGGGGAATTTCAAGAACCATGAGTGCTGTTG
TTGAACAGATGCATGACGAGCACGGTATGATATGGCCCCTTTCCATTGCACCCTTCCATATCATCATTACCATGGTGAAT
CCATCTCAGGAACAGATTTCTAAGGTTGGAGAGGAATTGTACGAACTTCTGAAGGAGAAATACGAGGTTCTCCTGGACGA
CCGGCAGGCTTCCCCTGGTGTTAAGTTCAAAGATGCAGACCTCATAGGAATACCTTTGAGGATCACCGTGGGTAAAAAAC
TCACAAAAGGGCTCATAGAACTGAAACTCCGGACCGAAAAACAACTCGTGGAAGTCTCTATCTCCGAAGGTTATGATTCA
GTCCTTGAAACCGTGGAAAAACTGCTGAAAAAATACGATCCTGCAAAGGTGGCGGAGGTAGATTAA

Upstream 100 bases:

>100_bases
AACTTCAATTAGATTATAACAAATCAGGAAAACTATGTCTGGACATGTTATAATCACAACCGAAATAGTCTATTTTGGTA
ACGAAAGGAGAATCGAATAT

Downstream 100 bases:

>100_bases
CATGGGATTCCTTTCTAAGATAAAAGAAGGTCTGAAAAAAACCCGTGATAGCGTATTCAGCAAAGTCAAGAACATTCTAA
GTCTCGAAAAACTTGATGCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MRFSQLYAPTLKEAPSDADLVSIKLLIRGGFVRKNAAGVYTYLPLGLRVLKKVEQIVREEMAAIGCQEILMPIIQPAELW
FESGRWDDYGPEMMKFKDRHERDFTLGPTHEELLTSIVRNELRSYRQFPLSLFQIANKYRDEIRPRFGLIRAREFLMKDA
YSFHTDWESLDKAYKDFYKAYGRIMERIGLKYLVVEADTGAIGGDESHEFNALADTGESTLLYCDCGYAASDEKAEYMML
SDEDPDVQEEKALELVETPGVRTVQEVADFLKVTPEQIVKSLLYRGKEGFVMALIRGDQELNESKLKAHLKDQTLTMATL
EEVLENFGVPIGFIGPVGMNDKVKIVADFTVKPLRNFVVGGMKEGYHYKGVCLGRDFSVDEWFDLKLAVEGDPCPKCGKP
MKMTKGIELGHIFKLGTKYSEKMNGYFTDENGENHPYIMGCYGWGISRTMSAVVEQMHDEHGMIWPLSIAPFHIIITMVN
PSQEQISKVGEELYELLKEKYEVLLDDRQASPGVKFKDADLIGIPLRITVGKKLTKGLIELKLRTEKQLVEVSISEGYDS
VLETVEKLLKKYDPAKVAEVD

Sequences:

>Translated_581_residues
MRFSQLYAPTLKEAPSDADLVSIKLLIRGGFVRKNAAGVYTYLPLGLRVLKKVEQIVREEMAAIGCQEILMPIIQPAELW
FESGRWDDYGPEMMKFKDRHERDFTLGPTHEELLTSIVRNELRSYRQFPLSLFQIANKYRDEIRPRFGLIRAREFLMKDA
YSFHTDWESLDKAYKDFYKAYGRIMERIGLKYLVVEADTGAIGGDESHEFNALADTGESTLLYCDCGYAASDEKAEYMML
SDEDPDVQEEKALELVETPGVRTVQEVADFLKVTPEQIVKSLLYRGKEGFVMALIRGDQELNESKLKAHLKDQTLTMATL
EEVLENFGVPIGFIGPVGMNDKVKIVADFTVKPLRNFVVGGMKEGYHYKGVCLGRDFSVDEWFDLKLAVEGDPCPKCGKP
MKMTKGIELGHIFKLGTKYSEKMNGYFTDENGENHPYIMGCYGWGISRTMSAVVEQMHDEHGMIWPLSIAPFHIIITMVN
PSQEQISKVGEELYELLKEKYEVLLDDRQASPGVKFKDADLIGIPLRITVGKKLTKGLIELKLRTEKQLVEVSISEGYDS
VLETVEKLLKKYDPAKVAEVD
>Mature_581_residues
MRFSQLYAPTLKEAPSDADLVSIKLLIRGGFVRKNAAGVYTYLPLGLRVLKKVEQIVREEMAAIGCQEILMPIIQPAELW
FESGRWDDYGPEMMKFKDRHERDFTLGPTHEELLTSIVRNELRSYRQFPLSLFQIANKYRDEIRPRFGLIRAREFLMKDA
YSFHTDWESLDKAYKDFYKAYGRIMERIGLKYLVVEADTGAIGGDESHEFNALADTGESTLLYCDCGYAASDEKAEYMML
SDEDPDVQEEKALELVETPGVRTVQEVADFLKVTPEQIVKSLLYRGKEGFVMALIRGDQELNESKLKAHLKDQTLTMATL
EEVLENFGVPIGFIGPVGMNDKVKIVADFTVKPLRNFVVGGMKEGYHYKGVCLGRDFSVDEWFDLKLAVEGDPCPKCGKP
MKMTKGIELGHIFKLGTKYSEKMNGYFTDENGENHPYIMGCYGWGISRTMSAVVEQMHDEHGMIWPLSIAPFHIIITMVN
PSQEQISKVGEELYELLKEKYEVLLDDRQASPGVKFKDADLIGIPLRITVGKKLTKGLIELKLRTEKQLVEVSISEGYDS
VLETVEKLLKKYDPAKVAEVD

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=39.8230088495575, Blast_Score=195, Evalue=8e-50,
Organism=Escherichia coli, GI1786392, Length=563, Percent_Identity=46.7140319715808, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI115532348, Length=205, Percent_Identity=40.4878048780488, Blast_Score=164, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=82, Percent_Identity=40.2439024390244, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=206, Percent_Identity=38.8349514563107, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24656200, Length=241, Percent_Identity=38.5892116182573, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_KOSOT (C5CFP9)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941401.1
- GeneID:   7968111
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1711
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 66031; Mature: 66031

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSQLYAPTLKEAPSDADLVSIKLLIRGGFVRKNAAGVYTYLPLGLRVLKKVEQIVREE
CCCHHHCCCHHHCCCCCCHHEEEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
MAAIGCQEILMPIIQPAELWFESGRWDDYGPEMMKFKDRHERDFTLGPTHEELLTSIVRN
HHHHCHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ELRSYRQFPLSLFQIANKYRDEIRPRFGLIRAREFLMKDAYSFHTDWESLDKAYKDFYKA
HHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YGRIMERIGLKYLVVEADTGAIGGDESHEFNALADTGESTLLYCDCGYAASDEKAEYMML
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCEEEE
SDEDPDVQEEKALELVETPGVRTVQEVADFLKVTPEQIVKSLLYRGKEGFVMALIRGDQE
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCH
LNESKLKAHLKDQTLTMATLEEVLENFGVPIGFIGPVGMNDKVKIVADFTVKPLRNFVVG
HCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHC
GMKEGYHYKGVCLGRDFSVDEWFDLKLAVEGDPCPKCGKPMKMTKGIELGHIFKLGTKYS
CCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCHHHCCCCHHHHHHHCCHHH
EKMNGYFTDENGENHPYIMGCYGWGISRTMSAVVEQMHDEHGMIWPLSIAPFHIIITMVN
HHCCCEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEC
PSQEQISKVGEELYELLKEKYEVLLDDRQASPGVKFKDADLIGIPLRITVGKKLTKGLIE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEEEEECCHHHHHHHHH
LKLRTEKQLVEVSISEGYDSVLETVEKLLKKYDPAKVAEVD
HHHHCCHHHHEEEHHCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRFSQLYAPTLKEAPSDADLVSIKLLIRGGFVRKNAAGVYTYLPLGLRVLKKVEQIVREE
CCCHHHCCCHHHCCCCCCHHEEEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
MAAIGCQEILMPIIQPAELWFESGRWDDYGPEMMKFKDRHERDFTLGPTHEELLTSIVRN
HHHHCHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ELRSYRQFPLSLFQIANKYRDEIRPRFGLIRAREFLMKDAYSFHTDWESLDKAYKDFYKA
HHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YGRIMERIGLKYLVVEADTGAIGGDESHEFNALADTGESTLLYCDCGYAASDEKAEYMML
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCEEEE
SDEDPDVQEEKALELVETPGVRTVQEVADFLKVTPEQIVKSLLYRGKEGFVMALIRGDQE
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCH
LNESKLKAHLKDQTLTMATLEEVLENFGVPIGFIGPVGMNDKVKIVADFTVKPLRNFVVG
HCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHC
GMKEGYHYKGVCLGRDFSVDEWFDLKLAVEGDPCPKCGKPMKMTKGIELGHIFKLGTKYS
CCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCHHHCCCCHHHHHHHCCHHH
EKMNGYFTDENGENHPYIMGCYGWGISRTMSAVVEQMHDEHGMIWPLSIAPFHIIITMVN
HHCCCEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEC
PSQEQISKVGEELYELLKEKYEVLLDDRQASPGVKFKDADLIGIPLRITVGKKLTKGLIE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEEEEECCHHHHHHHHH
LKLRTEKQLVEVSISEGYDSVLETVEKLLKKYDPAKVAEVD
HHHHCCHHHHEEEHHCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA