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The map label for this gene is gltX [H]

Identifier: 239618082

GI number: 239618082

Start: 1843630

End: 1845081

Strand: Reverse

Name: gltX [H]

Synonym: Kole_1714

Alternate gene names: 239618082

Gene position: 1845081-1843630 (Counterclockwise)

Preceding gene: 239618083

Following gene: 239618081

Centisome position: 80.15

GC content: 45.32

Gene sequence:

>1452_bases
ATGATCAGAGTAAGATTCGCGCCAAGTCCCACAGGATATTTGCACGTGGGTGGTGCAAGAACGGCGTTATTCAATTATCT
CTTTGCAAGGCATACCGGTGGAACATTCGTTTTGAGGATAGAGGACACCGATATCGAAAGATCAACAAAAGAATCTGAAG
AGAAACTGATGGAATCTCTCCTCTGGCTCGGAATTTCCTGGGACGAAGGTCCAGATGTTGGTGGCCCTTATGGCCCTTAC
AGGCAGAGCGAGCGAACGAAGATCTATCGTGAACACTGTGACAGACTTATCAAAGAAGGGAAGGCCTATCCAGTATACGC
TTACCCTGAAGAAATTGAAAAAATACATGATGAACTGCTTGCACAAGGCAAGCCGCCTCACTATTCTGAAGAATTGTTTA
AACAATTTGATACACCTGAAAGGAGAAAAGAGCTGGAAGAGCGCGGCCTCCAACCGGCGATCTTCTTCAAGATGCCACGA
AGGGAGTTCGTGTTCATAGACATCGTAAAAGGGAAAGTTGCTTTTAAAGAAGGGTCGATAGGTGACTTCGTTATACTGAG
GTCAAACGGCCTTCCAACGTATAACTTCGCCGTGGTTGTAGACGACATGACGATGAAAATTACACACGTTATTCGTGGCG
ACGACCATCTTTCAAACACCTTGAGGCAACTCGCCATATACGAAGCCTTTGAGGTTGAAGCGCCTGCTTTCGCGCACGTT
TCGACCATTCTCGGGCCTGATGGCAAAAAACTGAGCAAACGCCATGGACATACCTCCATCGAGGAATTCAGAGAAAAGGG
GTTCTTGCCTGAAGCTTTCGTAAACTTTCTTGTTCTCCTTGGATGGTCTCATCCGGACGGTAAAGAGATCATGGACGAAA
ACGAAATGATTGAAAAGTTCTCACTTGAACGCTTGAATTCAAGTCCTGCGGTGTTTGACGTTACCAAGGCACGCTGGATG
AACGGGGTTTACATACGCCAGACAAGTATAGAAAGAGTCACTGAGCTGGCTATCCCATATATCGTCAAGAAAGGGTATAT
GACGGAAGAACAAGCAAGAGCGAACATAAAATGGCTTAGAAAAGCCGTGGACTCTGTACGCAAAGGCGTCGAAACCCTTG
AAGAAATTCCCGAAAAACTAGCCGTTTACTTCAAAGGACCTGAAACCATGAAGCTCTCGATTAAAACCGAGGAGGAAAAA
CAAGTCGCACTTGCCCTTAAAAAAATCAAAGAACGCCTCGACGAGCTCGACAGTTGGGACCATAAAATAGTAGTAGATGT
TATTAGAAATGTGATAAAAGAGATTAAGCCAGACAGGAAGCTTTTCTATCACACCTTCAGGAAGGTTCTCACAGGTATGG
ACGAAGGGCCTGAACTCGTTGAAATTCTCTACCTGCTTGGAAGAAATAAAGTGATAGACAGAATAGATAGGGCGGTGGGT
AATCTGTGCTGA

Upstream 100 bases:

>100_bases
AGAAGTTCTTCAGCTCATTTTAGAAGGAAAGATAAGGGATGGTAAAACCATTGCCGGAATTCTCTTTGCTGATAGAATTA
GAAATAGGTGGTGATATACA

Downstream 100 bases:

>100_bases
GGATAACAAACACTCTCACAGGAAAAAAGGAAGAATTCATCCCGATAACCCCCGGAGAGGTCAGGATCTACGTTTGCGGA
CCCACGGTGTACAATTTTGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MIRVRFAPSPTGYLHVGGARTALFNYLFARHTGGTFVLRIEDTDIERSTKESEEKLMESLLWLGISWDEGPDVGGPYGPY
RQSERTKIYREHCDRLIKEGKAYPVYAYPEEIEKIHDELLAQGKPPHYSEELFKQFDTPERRKELEERGLQPAIFFKMPR
REFVFIDIVKGKVAFKEGSIGDFVILRSNGLPTYNFAVVVDDMTMKITHVIRGDDHLSNTLRQLAIYEAFEVEAPAFAHV
STILGPDGKKLSKRHGHTSIEEFREKGFLPEAFVNFLVLLGWSHPDGKEIMDENEMIEKFSLERLNSSPAVFDVTKARWM
NGVYIRQTSIERVTELAIPYIVKKGYMTEEQARANIKWLRKAVDSVRKGVETLEEIPEKLAVYFKGPETMKLSIKTEEEK
QVALALKKIKERLDELDSWDHKIVVDVIRNVIKEIKPDRKLFYHTFRKVLTGMDEGPELVEILYLLGRNKVIDRIDRAVG
NLC

Sequences:

>Translated_483_residues
MIRVRFAPSPTGYLHVGGARTALFNYLFARHTGGTFVLRIEDTDIERSTKESEEKLMESLLWLGISWDEGPDVGGPYGPY
RQSERTKIYREHCDRLIKEGKAYPVYAYPEEIEKIHDELLAQGKPPHYSEELFKQFDTPERRKELEERGLQPAIFFKMPR
REFVFIDIVKGKVAFKEGSIGDFVILRSNGLPTYNFAVVVDDMTMKITHVIRGDDHLSNTLRQLAIYEAFEVEAPAFAHV
STILGPDGKKLSKRHGHTSIEEFREKGFLPEAFVNFLVLLGWSHPDGKEIMDENEMIEKFSLERLNSSPAVFDVTKARWM
NGVYIRQTSIERVTELAIPYIVKKGYMTEEQARANIKWLRKAVDSVRKGVETLEEIPEKLAVYFKGPETMKLSIKTEEEK
QVALALKKIKERLDELDSWDHKIVVDVIRNVIKEIKPDRKLFYHTFRKVLTGMDEGPELVEILYLLGRNKVIDRIDRAVG
NLC
>Mature_483_residues
MIRVRFAPSPTGYLHVGGARTALFNYLFARHTGGTFVLRIEDTDIERSTKESEEKLMESLLWLGISWDEGPDVGGPYGPY
RQSERTKIYREHCDRLIKEGKAYPVYAYPEEIEKIHDELLAQGKPPHYSEELFKQFDTPERRKELEERGLQPAIFFKMPR
REFVFIDIVKGKVAFKEGSIGDFVILRSNGLPTYNFAVVVDDMTMKITHVIRGDDHLSNTLRQLAIYEAFEVEAPAFAHV
STILGPDGKKLSKRHGHTSIEEFREKGFLPEAFVNFLVLLGWSHPDGKEIMDENEMIEKFSLERLNSSPAVFDVTKARWM
NGVYIRQTSIERVTELAIPYIVKKGYMTEEQARANIKWLRKAVDSVRKGVETLEEIPEKLAVYFKGPETMKLSIKTEEEK
QVALALKKIKERLDELDSWDHKIVVDVIRNVIKEIKPDRKLFYHTFRKVLTGMDEGPELVEILYLLGRNKVIDRIDRAVG
NLC

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=493, Percent_Identity=31.0344827586207, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI62241042, Length=260, Percent_Identity=25.3846153846154, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=37.6299376299376, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI226510925, Length=252, Percent_Identity=31.3492063492063, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71993394, Length=478, Percent_Identity=31.1715481171548, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324540, Length=487, Percent_Identity=30.3901437371663, Blast_Score=226, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=225, Percent_Identity=27.5555555555556, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=116, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=34.7474747474747, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24649466, Length=227, Percent_Identity=25.9911894273128, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020752
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55770; Mature: 55770

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVRFAPSPTGYLHVGGARTALFNYLFARHTGGTFVLRIEDTDIERSTKESEEKLMESL
CEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
LWLGISWDEGPDVGGPYGPYRQSERTKIYREHCDRLIKEGKAYPVYAYPEEIEKIHDELL
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHH
AQGKPPHYSEELFKQFDTPERRKELEERGLQPAIFFKMPRREFVFIDIVKGKVAFKEGSI
HCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCEEEECCCCC
GDFVILRSNGLPTYNFAVVVDDMTMKITHVIRGDDHLSNTLRQLAIYEAFEVEAPAFAHV
CCEEEEECCCCCCEEEEEEEECHHEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
STILGPDGKKLSKRHGHTSIEEFREKGFLPEAFVNFLVLLGWSHPDGKEIMDENEMIEKF
HHHHCCCCHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHH
SLERLNSSPAVFDVTKARWMNGVYIRQTSIERVTELAIPYIVKKGYMTEEQARANIKWLR
HHHHCCCCCCEEEEHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KAVDSVRKGVETLEEIPEKLAVYFKGPETMKLSIKTEEEKQVALALKKIKERLDELDSWD
HHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
HKIVVDVIRNVIKEIKPDRKLFYHTFRKVLTGMDEGPELVEILYLLGRNKVIDRIDRAVG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHC
NLC
CCC
>Mature Secondary Structure
MIRVRFAPSPTGYLHVGGARTALFNYLFARHTGGTFVLRIEDTDIERSTKESEEKLMESL
CEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
LWLGISWDEGPDVGGPYGPYRQSERTKIYREHCDRLIKEGKAYPVYAYPEEIEKIHDELL
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHH
AQGKPPHYSEELFKQFDTPERRKELEERGLQPAIFFKMPRREFVFIDIVKGKVAFKEGSI
HCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCEEEECCCCC
GDFVILRSNGLPTYNFAVVVDDMTMKITHVIRGDDHLSNTLRQLAIYEAFEVEAPAFAHV
CCEEEEECCCCCCEEEEEEEECHHEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
STILGPDGKKLSKRHGHTSIEEFREKGFLPEAFVNFLVLLGWSHPDGKEIMDENEMIEKF
HHHHCCCCHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHH
SLERLNSSPAVFDVTKARWMNGVYIRQTSIERVTELAIPYIVKKGYMTEEQARANIKWLR
HHHHCCCCCCEEEEHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KAVDSVRKGVETLEEIPEKLAVYFKGPETMKLSIKTEEEKQVALALKKIKERLDELDSWD
HHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
HKIVVDVIRNVIKEIKPDRKLFYHTFRKVLTGMDEGPELVEILYLLGRNKVIDRIDRAVG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHC
NLC
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]