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The map label for this gene is ddl [H]

Identifier: 239618027

GI number: 239618027

Start: 1784173

End: 1785090

Strand: Reverse

Name: ddl [H]

Synonym: Kole_1656

Alternate gene names: 239618027

Gene position: 1785090-1784173 (Counterclockwise)

Preceding gene: 239618028

Following gene: 239618026

Centisome position: 77.54

GC content: 44.12

Gene sequence:

>918_bases
ATGAATGAACTTCGTCGAAGAATCACCGTGCTTTATGGTGGGCTTTCGAAGGAACGGCCTATTTCTTTGAAGAGTGGTTC
AAATGTTATAAATGCTCTGGAAAAGCTCGGATACATCGTGGATGCTTTCGACTTGAGAGCCGATAATATTACCGAACTTG
CAAAGCTCAGGGATACTGATCTGGTCTTCATCGCGCTCCACGGAAAATTTGGCGAAGATGGCAAAATTCAGTCCCTTCTC
GAACTATTCGGCATAGAGTACATTGGCTCAGATAGTCTCGTTAGCGCCATTTGTTTCAATAAAAACCTCACCTACAAACT
CCTTTCAAACACGATCACCATGCCCCGCTGGAAGACCATAACCTCAGAAGAAGAACTGGAAGGCTGGAAACACTTTCCTG
CGGTTATAAAGCCAGCGAACGAAGGCTCAAGCATCGGAGTCTATATATGCGACACATTTGAGGAGCTTCAAAACAAAACC
ACGAAAGTTCTAAAAGAATACAAAACGTTGATACTCGAAGAGTATATAAAAGGACGTGAGCTAACGATATCCATCATTGA
AAAAGGTGGCCAACCTGTCGTTCTGCCAATTCTCGAACTCAAACCCAAACGGCGCTTTTACGATTACGAAGCAAAATATA
CAGCGGGTCTCACGGAATTTATTGTTCCGGCCCCCATAGATGATAAACTCAAAGAGGAGATAAAGAAACTATCAAAAAGA
ATATTCACCGCTCTGGGTTGCAGGCATTTCGCCAGGATAGACGGCATACTTAAAGAAAATCAATTTTACTTTCTCGAAGT
AAATACCATCCCGGGCTTAACGGACCTCAGCGATCTGCCGGTGTCCGCCCGGGCCGCAGGGATGGGATTTGAAGAACTCA
TCGATTTGATAGTTAAAGAAGCCCTGAAACCTGATTGA

Upstream 100 bases:

>100_bases
AAGAAGTGCGAAGTTTGTGGAGACCCACTCTTCTTCAAGTTTAAAAGAAGAAGTAAAAAAGTACTTTACTGTGAAAAATG
CAAAAAGGAGTTCCCGATAA

Downstream 100 bases:

>100_bases
AACTGGAGGTGAAAGAATGGAACAAATGCATGGAACAACGATACTCGCTGTCAGAAGAAACGGTAAAACGGTAATTGCAG
GTGATGGGCAAATCACTCTC

Product: D-alanine/D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNELRRRITVLYGGLSKERPISLKSGSNVINALEKLGYIVDAFDLRADNITELAKLRDTDLVFIALHGKFGEDGKIQSLL
ELFGIEYIGSDSLVSAICFNKNLTYKLLSNTITMPRWKTITSEEELEGWKHFPAVIKPANEGSSIGVYICDTFEELQNKT
TKVLKEYKTLILEEYIKGRELTISIIEKGGQPVVLPILELKPKRRFYDYEAKYTAGLTEFIVPAPIDDKLKEEIKKLSKR
IFTALGCRHFARIDGILKENQFYFLEVNTIPGLTDLSDLPVSARAAGMGFEELIDLIVKEALKPD

Sequences:

>Translated_305_residues
MNELRRRITVLYGGLSKERPISLKSGSNVINALEKLGYIVDAFDLRADNITELAKLRDTDLVFIALHGKFGEDGKIQSLL
ELFGIEYIGSDSLVSAICFNKNLTYKLLSNTITMPRWKTITSEEELEGWKHFPAVIKPANEGSSIGVYICDTFEELQNKT
TKVLKEYKTLILEEYIKGRELTISIIEKGGQPVVLPILELKPKRRFYDYEAKYTAGLTEFIVPAPIDDKLKEEIKKLSKR
IFTALGCRHFARIDGILKENQFYFLEVNTIPGLTDLSDLPVSARAAGMGFEELIDLIVKEALKPD
>Mature_305_residues
MNELRRRITVLYGGLSKERPISLKSGSNVINALEKLGYIVDAFDLRADNITELAKLRDTDLVFIALHGKFGEDGKIQSLL
ELFGIEYIGSDSLVSAICFNKNLTYKLLSNTITMPRWKTITSEEELEGWKHFPAVIKPANEGSSIGVYICDTFEELQNKT
TKVLKEYKTLILEEYIKGRELTISIIEKGGQPVVLPILELKPKRRFYDYEAKYTAGLTEFIVPAPIDDKLKEEIKKLSKR
IFTALGCRHFARIDGILKENQFYFLEVNTIPGLTDLSDLPVSARAAGMGFEELIDLIVKEALKPD

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=310, Percent_Identity=35.8064516129032, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=28.9398280802292, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 34470; Mature: 34470

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELRRRITVLYGGLSKERPISLKSGSNVINALEKLGYIVDAFDLRADNITELAKLRDTD
CCHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
LVFIALHGKFGEDGKIQSLLELFGIEYIGSDSLVSAICFNKNLTYKLLSNTITMPRWKTI
EEEEEEECCCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCC
TSEEELEGWKHFPAVIKPANEGSSIGVYICDTFEELQNKTTKVLKEYKTLILEEYIKGRE
CCHHHHHHHHHCCHHEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LTISIIEKGGQPVVLPILELKPKRRFYDYEAKYTAGLTEFIVPAPIDDKLKEEIKKLSKR
EEEEEECCCCCEEEEEHCCCCCCCCCCCCCCHHHCCHHHHCCCCCCCHHHHHHHHHHHHH
IFTALGCRHFARIDGILKENQFYFLEVNTIPGLTDLSDLPVSARAAGMGFEELIDLIVKE
HHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
ALKPD
HCCCC
>Mature Secondary Structure
MNELRRRITVLYGGLSKERPISLKSGSNVINALEKLGYIVDAFDLRADNITELAKLRDTD
CCHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
LVFIALHGKFGEDGKIQSLLELFGIEYIGSDSLVSAICFNKNLTYKLLSNTITMPRWKTI
EEEEEEECCCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCC
TSEEELEGWKHFPAVIKPANEGSSIGVYICDTFEELQNKTTKVLKEYKTLILEEYIKGRE
CCHHHHHHHHHCCHHEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LTISIIEKGGQPVVLPILELKPKRRFYDYEAKYTAGLTEFIVPAPIDDKLKEEIKKLSKR
EEEEEECCCCCEEEEEHCCCCCCCCCCCCCCHHHCCHHHHCCCCCCCHHHHHHHHHHHHH
IFTALGCRHFARIDGILKENQFYFLEVNTIPGLTDLSDLPVSARAAGMGFEELIDLIVKE
HHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
ALKPD
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA