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The map label for this gene is topA [H]

Identifier: 239618028

GI number: 239618028

Start: 1785083

End: 1787308

Strand: Reverse

Name: topA [H]

Synonym: Kole_1657

Alternate gene names: 239618028

Gene position: 1787308-1785083 (Counterclockwise)

Preceding gene: 239618029

Following gene: 239618027

Centisome position: 77.64

GC content: 42.5

Gene sequence:

>2226_bases
TTGTCCAAAAAACTCGTTATAGTTGAATCCCCCGCAAAAGCCAAGACCATCGGAAGATACCTTGGCAAGGATTACGAGGT
AACTTCATCAAAAGGACACATTCGAGACCTTCCCGAATCTGAATTCGGTGTCGATCTCAACAGCCTCACCCCAACATACA
GAATTCTTAAGGGAAAGGAAAAGGTTGTCAAACAGCTGAAAGAAAAAGCAAAAGGAAAAGAGGTTCTTCTTGCTTCAGAT
ATGGATAGAGAAGGTGAAGCAATAGCGTGGCACGTGGCTCAAATACTCGGCCTTCCCAAAACAAAGAAAAACAGAATCAT
TTTCAGCGAAATCACCGAAAATGCGATAAAGCGAGCCGTTAAAAATCCAAGAACGATAGATATGAACAAAGTAAACGCTC
AGGTTGCCAGAAGAATCCTGGACAGGATAGTTGGTTACAAGCTAAGCCCATTGCTCTGGAAAACCGTTTCTCGCGGACTC
AGTGCCGGAAGGGTTCAAACGGTGGCTTTGAAGTTCATATCTGACCTGGAAAGCAAGATCGCGGCATTCAAGCCCCATAA
ATTTTATAAAATCTTCATGCGCGTCGGTGAACAAAAGATACAGCTTCTCGAAATAAATGGAAAGAAATTCAGCAATAAAT
CCATCAGAACGGAAGAAGAACGCGATAAAATCCTAAACGAACTTAAACAATCCGTTTTTGTAGTTTCCAGTATTGAAACA
ACAAGAAAAAAGATCAAACCACCTGAACCATTCATAACCTCGACCATGCAGCAAACTGCCATAAACGAACTCGGCTGGAG
CGCTTCGAAGACCATGAAAGTAGCACAACAACTCTACGAAGGCATCGATACCCCGGAAGGTCATATAGCCTTCATCACCT
ACATGAGAACAGATTCAACCAGAATATCGGAGGAAGCAAAAGAAAAAGCGAAAGTGTTCATTCTGGAAAAATTCGGAGAG
AAATACCTCGGAACTGCAAAAACGAAGAAATCCAGAAAGAAAAATGTCCAGGATGCCCACGAAGCTATAAGGCCTACATA
TCCTGAACGCACACCGGAAGAGGTTAAGGCGAAAAAACTTCTCTCCGGTGATCACCTTCGCCTCTACGCACTCGTATGGA
AACGTTTCTTCGCTTCGCAGATGGCAGCGGCGGAATATGATGTAACTACGATAAAAATAGCCGATGAAAATGGGAAATAC
GTCTTCAAATACACCACCAGAAACTGTGTATTTGACGGGTTTGAACGTATTCTTAAGAAAACAAACGGTAACCACGAAAC
CGCTATCGTTCCAGAAGGGAAAACAATAAAACCAGACACCTTTCTATGGGAAGAAGACCAGACGAAACCCCCGGCAAGGT
ACACAGAAGCCTCACTCGTAAGGGAATTAGAGAAAAAGGGCATAGGTCGTCCCTCAACCTACGCTACAATAATCTCTACA
TTGCTCGAAAGAAAGTACGTGGTAAAATCCGGCAGAGAGCTCAGGCCAACGCTTTTAGGGAACATAGTTGCCGACTTTCT
CGGAAAAAGCTTCCCGGATATTATAAATGAAAAATTCACGGCTAACATGGAAGAAGAACTGGATGAGATTGAAAACGCCA
AACGCCAATGGAAGGAAGTTGTTTTAAGGTTTTACGAAAACTTCGCGCAGGATCTGAACAAGCTAGAACAGGATATAAAG
AACGGAAAGTTGAAAATAGAATACCCGACCGACAGAAAATGCGATTGCGGTGGCGAATTCAAGGTCGTATTTGGAAGATA
CGGCGGCTATTTGAAATGTTCCGAATGCGGCAAAAATCAATCTCTTGACATGACTGTATTCGCCCCCATCATAGAAGGCA
AGGTAATCCTTCACGACATTATAGAAACCAACAACAAAAAGAACGAATTAGATGAAACCTGTCCTGAATGCGGCTCCAAA
CTTGTCATAAGAAAAGGAAGATATGGCTCATTCATAGCATGTTCTGCATATCCTAAATGTACCTACACACGAGACATATA
CGTAGATGCAAAATGCCCAGAATGCGGCGGTTCTGTCAAAAAAATGCGAAGCAAAAAGGGAAAAACCTACTTTAAATGTG
TAGATTGCGGAAAGATGTTTTGGCGCGAACCCGCTGGCAAGAAGTGCGAAGTTTGTGGAGACCCACTCTTCTTCAAGTTT
AAAAGAAGAAGTAAAAAAGTACTTTACTGTGAAAAATGCAAAAAGGAGTTCCCGATAAATGAATGA

Upstream 100 bases:

>100_bases
ATCAAACGCTTCTGGTTACGGTTGGAGTAATTACGACAGGTTCTTAAACCGCAGAATCTTCATGAAAAACCGTGACAGTT
CAGCACAATGAGGTGATATC

Downstream 100 bases:

>100_bases
ACTTCGTCGAAGAATCACCGTGCTTTATGGTGGGCTTTCGAAGGAACGGCCTATTTCTTTGAAGAGTGGTTCAAATGTTA
TAAATGCTCTGGAAAAGCTC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 741; Mature: 740

Protein sequence:

>741_residues
MSKKLVIVESPAKAKTIGRYLGKDYEVTSSKGHIRDLPESEFGVDLNSLTPTYRILKGKEKVVKQLKEKAKGKEVLLASD
MDREGEAIAWHVAQILGLPKTKKNRIIFSEITENAIKRAVKNPRTIDMNKVNAQVARRILDRIVGYKLSPLLWKTVSRGL
SAGRVQTVALKFISDLESKIAAFKPHKFYKIFMRVGEQKIQLLEINGKKFSNKSIRTEEERDKILNELKQSVFVVSSIET
TRKKIKPPEPFITSTMQQTAINELGWSASKTMKVAQQLYEGIDTPEGHIAFITYMRTDSTRISEEAKEKAKVFILEKFGE
KYLGTAKTKKSRKKNVQDAHEAIRPTYPERTPEEVKAKKLLSGDHLRLYALVWKRFFASQMAAAEYDVTTIKIADENGKY
VFKYTTRNCVFDGFERILKKTNGNHETAIVPEGKTIKPDTFLWEEDQTKPPARYTEASLVRELEKKGIGRPSTYATIIST
LLERKYVVKSGRELRPTLLGNIVADFLGKSFPDIINEKFTANMEEELDEIENAKRQWKEVVLRFYENFAQDLNKLEQDIK
NGKLKIEYPTDRKCDCGGEFKVVFGRYGGYLKCSECGKNQSLDMTVFAPIIEGKVILHDIIETNNKKNELDETCPECGSK
LVIRKGRYGSFIACSAYPKCTYTRDIYVDAKCPECGGSVKKMRSKKGKTYFKCVDCGKMFWREPAGKKCEVCGDPLFFKF
KRRSKKVLYCEKCKKEFPINE

Sequences:

>Translated_741_residues
MSKKLVIVESPAKAKTIGRYLGKDYEVTSSKGHIRDLPESEFGVDLNSLTPTYRILKGKEKVVKQLKEKAKGKEVLLASD
MDREGEAIAWHVAQILGLPKTKKNRIIFSEITENAIKRAVKNPRTIDMNKVNAQVARRILDRIVGYKLSPLLWKTVSRGL
SAGRVQTVALKFISDLESKIAAFKPHKFYKIFMRVGEQKIQLLEINGKKFSNKSIRTEEERDKILNELKQSVFVVSSIET
TRKKIKPPEPFITSTMQQTAINELGWSASKTMKVAQQLYEGIDTPEGHIAFITYMRTDSTRISEEAKEKAKVFILEKFGE
KYLGTAKTKKSRKKNVQDAHEAIRPTYPERTPEEVKAKKLLSGDHLRLYALVWKRFFASQMAAAEYDVTTIKIADENGKY
VFKYTTRNCVFDGFERILKKTNGNHETAIVPEGKTIKPDTFLWEEDQTKPPARYTEASLVRELEKKGIGRPSTYATIIST
LLERKYVVKSGRELRPTLLGNIVADFLGKSFPDIINEKFTANMEEELDEIENAKRQWKEVVLRFYENFAQDLNKLEQDIK
NGKLKIEYPTDRKCDCGGEFKVVFGRYGGYLKCSECGKNQSLDMTVFAPIIEGKVILHDIIETNNKKNELDETCPECGSK
LVIRKGRYGSFIACSAYPKCTYTRDIYVDAKCPECGGSVKKMRSKKGKTYFKCVDCGKMFWREPAGKKCEVCGDPLFFKF
KRRSKKVLYCEKCKKEFPINE
>Mature_740_residues
SKKLVIVESPAKAKTIGRYLGKDYEVTSSKGHIRDLPESEFGVDLNSLTPTYRILKGKEKVVKQLKEKAKGKEVLLASDM
DREGEAIAWHVAQILGLPKTKKNRIIFSEITENAIKRAVKNPRTIDMNKVNAQVARRILDRIVGYKLSPLLWKTVSRGLS
AGRVQTVALKFISDLESKIAAFKPHKFYKIFMRVGEQKIQLLEINGKKFSNKSIRTEEERDKILNELKQSVFVVSSIETT
RKKIKPPEPFITSTMQQTAINELGWSASKTMKVAQQLYEGIDTPEGHIAFITYMRTDSTRISEEAKEKAKVFILEKFGEK
YLGTAKTKKSRKKNVQDAHEAIRPTYPERTPEEVKAKKLLSGDHLRLYALVWKRFFASQMAAAEYDVTTIKIADENGKYV
FKYTTRNCVFDGFERILKKTNGNHETAIVPEGKTIKPDTFLWEEDQTKPPARYTEASLVRELEKKGIGRPSTYATIISTL
LERKYVVKSGRELRPTLLGNIVADFLGKSFPDIINEKFTANMEEELDEIENAKRQWKEVVLRFYENFAQDLNKLEQDIKN
GKLKIEYPTDRKCDCGGEFKVVFGRYGGYLKCSECGKNQSLDMTVFAPIIEGKVILHDIIETNNKKNELDETCPECGSKL
VIRKGRYGSFIACSAYPKCTYTRDIYVDAKCPECGGSVKKMRSKKGKTYFKCVDCGKMFWREPAGKKCEVCGDPLFFKFK
RRSKKVLYCEKCKKEFPINE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=683, Percent_Identity=22.98682284041, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI10835218, Length=652, Percent_Identity=22.5460122699387, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1787529, Length=782, Percent_Identity=36.1892583120205, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI48994931, Length=176, Percent_Identity=28.9772727272727, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32563869, Length=570, Percent_Identity=27.3684210526316, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=559, Percent_Identity=25.2236135957066, Blast_Score=114, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=283, Percent_Identity=24.3816254416961, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24640096, Length=511, Percent_Identity=24.853228962818, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585251, Length=713, Percent_Identity=23.7026647966339, Blast_Score=109, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 84731; Mature: 84600

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLVIVESPAKAKTIGRYLGKDYEVTSSKGHIRDLPESEFGVDLNSLTPTYRILKGKE
CCCCEEEEECCHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHCCCHHCCCCHHHHHCCHH
KVVKQLKEKAKGKEVLLASDMDREGEAIAWHVAQILGLPKTKKNRIIFSEITENAIKRAV
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHH
KNPRTIDMNKVNAQVARRILDRIVGYKLSPLLWKTVSRGLSAGRVQTVALKFISDLESKI
CCCCEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AAFKPHKFYKIFMRVGEQKIQLLEINGKKFSNKSIRTEEERDKILNELKQSVFVVSSIET
HHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRKKIKPPEPFITSTMQQTAINELGWSASKTMKVAQQLYEGIDTPEGHIAFITYMRTDST
HHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCH
RISEEAKEKAKVFILEKFGEKYLGTAKTKKSRKKNVQDAHEAIRPTYPERTPEEVKAKKL
HHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHH
LSGDHLRLYALVWKRFFASQMAAAEYDVTTIKIADENGKYVFKYTTRNCVFDGFERILKK
HCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHH
TNGNHETAIVPEGKTIKPDTFLWEEDQTKPPARYTEASLVRELEKKGIGRPSTYATIIST
CCCCCCEEEECCCCEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LLERKYVVKSGRELRPTLLGNIVADFLGKSFPDIINEKFTANMEEELDEIENAKRQWKEV
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VLRFYENFAQDLNKLEQDIKNGKLKIEYPTDRKCDCGGEFKVVFGRYGGYLKCSECGKNQ
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEECCCCCCC
SLDMTVFAPIIEGKVILHDIIETNNKKNELDETCPECGSKLVIRKGRYGSFIACSAYPKC
CCCEEEEHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCEEEECCCCCC
TYTRDIYVDAKCPECGGSVKKMRSKKGKTYFKCVDCGKMFWREPAGKKCEVCGDPLFFKF
EEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHHHH
KRRSKKVLYCEKCKKEFPINE
HHCCCCEEEHHHHHCCCCCCC
>Mature Secondary Structure 
SKKLVIVESPAKAKTIGRYLGKDYEVTSSKGHIRDLPESEFGVDLNSLTPTYRILKGKE
CCCEEEEECCHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHCCCHHCCCCHHHHHCCHH
KVVKQLKEKAKGKEVLLASDMDREGEAIAWHVAQILGLPKTKKNRIIFSEITENAIKRAV
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHH
KNPRTIDMNKVNAQVARRILDRIVGYKLSPLLWKTVSRGLSAGRVQTVALKFISDLESKI
CCCCEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AAFKPHKFYKIFMRVGEQKIQLLEINGKKFSNKSIRTEEERDKILNELKQSVFVVSSIET
HHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRKKIKPPEPFITSTMQQTAINELGWSASKTMKVAQQLYEGIDTPEGHIAFITYMRTDST
HHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCH
RISEEAKEKAKVFILEKFGEKYLGTAKTKKSRKKNVQDAHEAIRPTYPERTPEEVKAKKL
HHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHH
LSGDHLRLYALVWKRFFASQMAAAEYDVTTIKIADENGKYVFKYTTRNCVFDGFERILKK
HCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHH
TNGNHETAIVPEGKTIKPDTFLWEEDQTKPPARYTEASLVRELEKKGIGRPSTYATIIST
CCCCCCEEEECCCCEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LLERKYVVKSGRELRPTLLGNIVADFLGKSFPDIINEKFTANMEEELDEIENAKRQWKEV
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VLRFYENFAQDLNKLEQDIKNGKLKIEYPTDRKCDCGGEFKVVFGRYGGYLKCSECGKNQ
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEECCCCCCC
SLDMTVFAPIIEGKVILHDIIETNNKKNELDETCPECGSKLVIRKGRYGSFIACSAYPKC
CCCEEEEHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCEEEECCCCCC
TYTRDIYVDAKCPECGGSVKKMRSKKGKTYFKCVDCGKMFWREPAGKKCEVCGDPLFFKF
EEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHHHH
KRRSKKVLYCEKCKKEFPINE
HHCCCCEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA