Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 239617993

GI number: 239617993

Start: 1740822

End: 1741193

Strand: Reverse

Name: gcvH [H]

Synonym: Kole_1621

Alternate gene names: 239617993

Gene position: 1741193-1740822 (Counterclockwise)

Preceding gene: 239617994

Following gene: 239617992

Centisome position: 75.63

GC content: 39.52

Gene sequence:

>372_bases
ATGAAAAAGTTTGCAAAGTCTCATGAATGGATTTCCATCGAAGGTGAAATTGGAACAGTAGGCATAACGAATTACGCCCA
GGAACATCTCGGAGATATTGTTTATGTTGAACTTCCGGAAATTGGAAAAACTGTGAGTAAAGAAGAGGTTGTTTGTACGG
TTGAATCTGTTAAGGCTGCAAGTGATATTTACGCTCCTGTATCCGGTGAAATTGTTGAAATCAATGAAGAACTTGACACG
ACACCAGAACTCATAAATCAGGATGCAGAAGGAGAAGGCTGGCTTTTTAAGATAAAGCTTTCAAATTCTGATGAACTCAA
CGAGCTCATGGACGAAGAGGAGTACAAGGCTTTTTGTAAAGAGGAGGAATGA

Upstream 100 bases:

>100_bases
ATTAAGATAAGGAGCAAGATGGTAAAAGCGGTTGTAACCAAAACACCATTTTATAGAGGTTCAGTAAAATCAAAAGGTTA
ATAAAAGGAGGTTATCTGTC

Downstream 100 bases:

>100_bases
CATGAAAGAACGTCATTCTTACATTCCTCACACCCCGGAAGAAATAAAATCGATGTTAGAAACGATAGGAGTTCCCAGTA
TTGAGGAGCTTTTTTCAGAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MKKFAKSHEWISIEGEIGTVGITNYAQEHLGDIVYVELPEIGKTVSKEEVVCTVESVKAASDIYAPVSGEIVEINEELDT
TPELINQDAEGEGWLFKIKLSNSDELNELMDEEEYKAFCKEEE

Sequences:

>Translated_123_residues
MKKFAKSHEWISIEGEIGTVGITNYAQEHLGDIVYVELPEIGKTVSKEEVVCTVESVKAASDIYAPVSGEIVEINEELDT
TPELINQDAEGEGWLFKIKLSNSDELNELMDEEEYKAFCKEEE
>Mature_123_residues
MKKFAKSHEWISIEGEIGTVGITNYAQEHLGDIVYVELPEIGKTVSKEEVVCTVESVKAASDIYAPVSGEIVEINEELDT
TPELINQDAEGEGWLFKIKLSNSDELNELMDEEEYKAFCKEEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=51.6666666666667, Blast_Score=135, Evalue=6e-33,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=51.6666666666667, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=52.8925619834711, Blast_Score=128, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=49.1803278688525, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=49.1666666666667, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319272, Length=117, Percent_Identity=43.5897435897436, Blast_Score=104, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=45.7627118644068, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13815; Mature: 13815

Theoretical pI: Translated: 3.90; Mature: 3.90

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFAKSHEWISIEGEIGTVGITNYAQEHLGDIVYVELPEIGKTVSKEEVVCTVESVKAA
CCCCCCCCCEEEEECCEEEEECCHHHHHHCCCEEEEECCCCCCCCCCCCEEEEHHHHHHH
SDIYAPVSGEIVEINEELDTTPELINQDAEGEGWLFKIKLSNSDELNELMDEEEYKAFCK
HHCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCCHHHHHHHCHHHHHHHCC
EEE
CCC
>Mature Secondary Structure
MKKFAKSHEWISIEGEIGTVGITNYAQEHLGDIVYVELPEIGKTVSKEEVVCTVESVKAA
CCCCCCCCCEEEEECCEEEEECCHHHHHHCCCEEEEECCCCCCCCCCCCEEEEHHHHHHH
SDIYAPVSGEIVEINEELDTTPELINQDAEGEGWLFKIKLSNSDELNELMDEEEYKAFCK
HHCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCCHHHHHHHCHHHHHHHCC
EEE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]