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The map label for this gene is clpB [H]

Identifier: 239617275

GI number: 239617275

Start: 931045

End: 933429

Strand: Reverse

Name: clpB [H]

Synonym: Kole_0884

Alternate gene names: 239617275

Gene position: 933429-931045 (Counterclockwise)

Preceding gene: 239617276

Following gene: 239617274

Centisome position: 40.55

GC content: 44.4

Gene sequence:

>2385_bases
ATGATAAATTATGAAGAATACACGGAGAAGGCGAAAAAAATCCTGATGGATGTTCAGGATATATTGACAAGATATCGCCA
GAATCAATTTGGCAGCGAGCATATTTTGCTTGCTATCCTCGAAGATGAGGATAACTTTGCTTATGAGATTCTGAAAGAGT
TGAAGGTTAATATGAGATCCCTTCGCCGTGATGTCGAAGAGGTTATTGCGCGCTATGGTGGGCGTGTTGAATCTTCCAAT
CAGATTTATATTACACCCGACGCACGCCACATCATAGAAGCAGCCAGAAACGAAGCCAAAAGAATGCACGATGCCAAAGT
TGGTACGGAACATCTGTTACTTGCTATGCTTAGAGAGGTTTCGTCAGTAGCGGCAAGAATCCTGATGAAACACGGATTGA
ACGTTGATACCGTTTACAATGCCATACTGAGTGCCAGGCAAAAGGGTGAAGCGTCCGAAAACGAGAATGTTGATGTTCTC
AAGCGTTTCACCATAGACTTAACCGATTTGGCAAAGCAAGGAAAACTGATGCCTGTTATAGGCAGGGATGAAGAAATAAG
AAGGGTCATCCAGATACTCGGTAGAAAGACCAAAAACAATCCTGCCTTAGTCGGGGAACCTGGTGTTGGTAAAACCGCAA
TTGTCGAGGGGTTGGCTCAGAGAATCGTTGATGGTGACGTTCCCGAATACCTGAAAAACAAAAAAGTGCTTGCTCTGGAT
ATGGGAAGAGTTGTTGCTGGAACCAAGTTCCGTGGAGAATTTGAAGAGAGAATGAAGGGGATCATTGATGCGGTGAAACG
TCTTGCTGGAGAAGTGATTTTGTTCATAGATGAGATTCATACCGTTGTGGGTGCCGGTTCTGCGGAGGGTTCAATGGATG
CTGCCAACCTTATGAAACCAGCTCTCGCGAGAGGAGAGCTTCAATGTATAGGGGCAACTACTCTCGATGAGTATAGAAAG
TACATCGAAAAAGACAGGGCTCTTGAAAGACGATTCCAGCCAGTTTATGTAGACGAACCTTCAAGGGATGAGGCGCTGGA
AATTGTAAAAGGATTGCGAGAGTCGTATGAAAAGCATCACCAGGTTAAGATAACGGATAAAGCTCTTGAGGCGGCCGTTG
ATCTAAGTATGAAATACATAACAGACAGATACCTACCAGACAAGGCTATTGATCTTATAGATGAAGCTGCATCTTATGTA
CGCCTTAAAGCCGGATATCTTCCGGATGATTTGAGGAAGATGGAAAAAGAGCTCCAGGAGCTCGACAACAGGATATCCGA
GCTCGTTCAGGAAGGTGAATACCAGAAAGCCGCGGAACTCAAAACGAAATTCGAGAAAATCAGAAAAGAATACGAAGCCA
GAAAGGCTGAATGGTTCAAGAGTGACAACGCTCAGAACAATGAAGTTACGGAAGAAATAATTGCTTCAATCGTCGAGCAG
TGGACCGGAATACCAGCCGGGAAACTTCTCGAATCTGAAAGGGATAAATTGAAAAATCTCGAAAAGCTAATTCACGAACG
TTTCATAGATCAGGAAGAGGCTGTTAGTGCGGTTGCCCATACCATAAGAAGGGCACGTGCTGGTCTAAAAGCACCGAACC
GACCATGGGGATCATTCCTATTCCTTGGACCAACAGGTGTCGGGAAGACGGAACTTGCGAAAACACTGGCAAATATACTT
TTTGGTAGCGAAGATGCGATGATAAGAATCGATATGTCCGAATACATGGAAAAGCACTCTGTATCGAGGCTTATTGGTGC
GCCTCCGGGATACGTCGGATACGAAGAAGGTGGTCAATTAACTGAGGCGGTTAGAAGAAGACCCTACTCCGTCATATTAC
TTGATGAAGTTGAAAAAGCACATCCTGATGTTCACAATGTTCTACTTCAAGTGATGGACGATGGAAGATTAACTGATGGA
AAAGGAAAAACCGTTAATTTCTCCAACACCATACTCATCATGACCAGTAATGTGGGTTCTGAAGAGATGACGAAGGGTAA
ATTCGACGAAAACACAACGGCACTTGTCGAACAGAAACTTAAGTCAGCATTCAAACCGGAGTTCCTGAATAGACTCGATG
CCATCGTGTTCTTCAAGCCGTTGAGCAAAGAGCACCTGAGAGAGATCGTTAAATTGAGATTGAAAGAGGTCGAGGAAAAA
CTCAGCGAACAGGGTATATCCATGCGTTACACCGATAAAGCCGTCGATTACCTTGCGGATAGAGGATATGATCCCGTATA
TGGCGCAAGGCCGATAAGGAGGCTCGTTGAAAGAGATGTGGAAGGACAGATTGCCGACATGATAATCAGCGGCGAGCTTT
CTGAAAACGATGTTGTCGTTGTGGGTGCCGATGATTTCGGCATAAAGATTTACAAAGAAGAATAA

Upstream 100 bases:

>100_bases
AGAAGTCAAGAAAGAAACGGTAAGTGTGAAAGTTGAGTAATTGAGATGAAATCATACGGGGCGCCTTTAAGGCGCCCTTT
TTAAAACTGGAGGTGAGAGA

Downstream 100 bases:

>100_bases
TCATTTCGAAAGTTCTAAGGCATACGCGGCGGGATATTCCTGCCGCGTACTTTTTTTGTTGTATAATTGGTTCTGTCAAA
ATTCTTATGGAGATGATAGC

Product: ATPase AAA-2 domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 794; Mature: 794

Protein sequence:

>794_residues
MINYEEYTEKAKKILMDVQDILTRYRQNQFGSEHILLAILEDEDNFAYEILKELKVNMRSLRRDVEEVIARYGGRVESSN
QIYITPDARHIIEAARNEAKRMHDAKVGTEHLLLAMLREVSSVAARILMKHGLNVDTVYNAILSARQKGEASENENVDVL
KRFTIDLTDLAKQGKLMPVIGRDEEIRRVIQILGRKTKNNPALVGEPGVGKTAIVEGLAQRIVDGDVPEYLKNKKVLALD
MGRVVAGTKFRGEFEERMKGIIDAVKRLAGEVILFIDEIHTVVGAGSAEGSMDAANLMKPALARGELQCIGATTLDEYRK
YIEKDRALERRFQPVYVDEPSRDEALEIVKGLRESYEKHHQVKITDKALEAAVDLSMKYITDRYLPDKAIDLIDEAASYV
RLKAGYLPDDLRKMEKELQELDNRISELVQEGEYQKAAELKTKFEKIRKEYEARKAEWFKSDNAQNNEVTEEIIASIVEQ
WTGIPAGKLLESERDKLKNLEKLIHERFIDQEEAVSAVAHTIRRARAGLKAPNRPWGSFLFLGPTGVGKTELAKTLANIL
FGSEDAMIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLDEVEKAHPDVHNVLLQVMDDGRLTDG
KGKTVNFSNTILIMTSNVGSEEMTKGKFDENTTALVEQKLKSAFKPEFLNRLDAIVFFKPLSKEHLREIVKLRLKEVEEK
LSEQGISMRYTDKAVDYLADRGYDPVYGARPIRRLVERDVEGQIADMIISGELSENDVVVVGADDFGIKIYKEE

Sequences:

>Translated_794_residues
MINYEEYTEKAKKILMDVQDILTRYRQNQFGSEHILLAILEDEDNFAYEILKELKVNMRSLRRDVEEVIARYGGRVESSN
QIYITPDARHIIEAARNEAKRMHDAKVGTEHLLLAMLREVSSVAARILMKHGLNVDTVYNAILSARQKGEASENENVDVL
KRFTIDLTDLAKQGKLMPVIGRDEEIRRVIQILGRKTKNNPALVGEPGVGKTAIVEGLAQRIVDGDVPEYLKNKKVLALD
MGRVVAGTKFRGEFEERMKGIIDAVKRLAGEVILFIDEIHTVVGAGSAEGSMDAANLMKPALARGELQCIGATTLDEYRK
YIEKDRALERRFQPVYVDEPSRDEALEIVKGLRESYEKHHQVKITDKALEAAVDLSMKYITDRYLPDKAIDLIDEAASYV
RLKAGYLPDDLRKMEKELQELDNRISELVQEGEYQKAAELKTKFEKIRKEYEARKAEWFKSDNAQNNEVTEEIIASIVEQ
WTGIPAGKLLESERDKLKNLEKLIHERFIDQEEAVSAVAHTIRRARAGLKAPNRPWGSFLFLGPTGVGKTELAKTLANIL
FGSEDAMIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLDEVEKAHPDVHNVLLQVMDDGRLTDG
KGKTVNFSNTILIMTSNVGSEEMTKGKFDENTTALVEQKLKSAFKPEFLNRLDAIVFFKPLSKEHLREIVKLRLKEVEEK
LSEQGISMRYTDKAVDYLADRGYDPVYGARPIRRLVERDVEGQIADMIISGELSENDVVVVGADDFGIKIYKEE
>Mature_794_residues
MINYEEYTEKAKKILMDVQDILTRYRQNQFGSEHILLAILEDEDNFAYEILKELKVNMRSLRRDVEEVIARYGGRVESSN
QIYITPDARHIIEAARNEAKRMHDAKVGTEHLLLAMLREVSSVAARILMKHGLNVDTVYNAILSARQKGEASENENVDVL
KRFTIDLTDLAKQGKLMPVIGRDEEIRRVIQILGRKTKNNPALVGEPGVGKTAIVEGLAQRIVDGDVPEYLKNKKVLALD
MGRVVAGTKFRGEFEERMKGIIDAVKRLAGEVILFIDEIHTVVGAGSAEGSMDAANLMKPALARGELQCIGATTLDEYRK
YIEKDRALERRFQPVYVDEPSRDEALEIVKGLRESYEKHHQVKITDKALEAAVDLSMKYITDRYLPDKAIDLIDEAASYV
RLKAGYLPDDLRKMEKELQELDNRISELVQEGEYQKAAELKTKFEKIRKEYEARKAEWFKSDNAQNNEVTEEIIASIVEQ
WTGIPAGKLLESERDKLKNLEKLIHERFIDQEEAVSAVAHTIRRARAGLKAPNRPWGSFLFLGPTGVGKTELAKTLANIL
FGSEDAMIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLDEVEKAHPDVHNVLLQVMDDGRLTDG
KGKTVNFSNTILIMTSNVGSEEMTKGKFDENTTALVEQKLKSAFKPEFLNRLDAIVFFKPLSKEHLREIVKLRLKEVEEK
LSEQGISMRYTDKAVDYLADRGYDPVYGARPIRRLVERDVEGQIADMIISGELSENDVVVVGADDFGIKIYKEE

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=299, Percent_Identity=39.7993311036789, Blast_Score=203, Evalue=6e-52,
Organism=Escherichia coli, GI1788943, Length=842, Percent_Identity=48.2185273159145, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=788, Percent_Identity=38.9593908629442, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6320464, Length=695, Percent_Identity=46.0431654676259, Blast_Score=620, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6323002, Length=864, Percent_Identity=37.3842592592593, Blast_Score=543, Evalue=1e-155,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 89930; Mature: 89930

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50151 UVR ; PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINYEEYTEKAKKILMDVQDILTRYRQNQFGSEHILLAILEDEDNFAYEILKELKVNMRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
LRRDVEEVIARYGGRVESSNQIYITPDARHIIEAARNEAKRMHDAKVGTEHLLLAMLREV
HHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SSVAARILMKHGLNVDTVYNAILSARQKGEASENENVDVLKRFTIDLTDLAKQGKLMPVI
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEC
GRDEEIRRVIQILGRKTKNNPALVGEPGVGKTAIVEGLAQRIVDGDVPEYLKNKKVLALD
CCCHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCEEEEE
MGRVVAGTKFRGEFEERMKGIIDAVKRLAGEVILFIDEIHTVVGAGSAEGSMDAANLMKP
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ALARGELQCIGATTLDEYRKYIEKDRALERRFQPVYVDEPSRDEALEIVKGLRESYEKHH
HHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHC
QVKITDKALEAAVDLSMKYITDRYLPDKAIDLIDEAASYVRLKAGYLPDDLRKMEKELQE
CEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LDNRISELVQEGEYQKAAELKTKFEKIRKEYEARKAEWFKSDNAQNNEVTEEIIASIVEQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
WTGIPAGKLLESERDKLKNLEKLIHERFIDQEEAVSAVAHTIRRARAGLKAPNRPWGSFL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FLGPTGVGKTELAKTLANILFGSEDAMIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQL
EECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TEAVRRRPYSVILLDEVEKAHPDVHNVLLQVMDDGRLTDGKGKTVNFSNTILIMTSNVGS
HHHHHHCCCEEEEECCHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCCCC
EEMTKGKFDENTTALVEQKLKSAFKPEFLNRLDAIVFFKPLSKEHLREIVKLRLKEVEEK
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSEQGISMRYTDKAVDYLADRGYDPVYGARPIRRLVERDVEGQIADMIISGELSENDVVV
HHHCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHEECCCCCCCEEE
VGADDFGIKIYKEE
EECCCCCEEEEECC
>Mature Secondary Structure
MINYEEYTEKAKKILMDVQDILTRYRQNQFGSEHILLAILEDEDNFAYEILKELKVNMRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
LRRDVEEVIARYGGRVESSNQIYITPDARHIIEAARNEAKRMHDAKVGTEHLLLAMLREV
HHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SSVAARILMKHGLNVDTVYNAILSARQKGEASENENVDVLKRFTIDLTDLAKQGKLMPVI
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEC
GRDEEIRRVIQILGRKTKNNPALVGEPGVGKTAIVEGLAQRIVDGDVPEYLKNKKVLALD
CCCHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCEEEEE
MGRVVAGTKFRGEFEERMKGIIDAVKRLAGEVILFIDEIHTVVGAGSAEGSMDAANLMKP
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ALARGELQCIGATTLDEYRKYIEKDRALERRFQPVYVDEPSRDEALEIVKGLRESYEKHH
HHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHC
QVKITDKALEAAVDLSMKYITDRYLPDKAIDLIDEAASYVRLKAGYLPDDLRKMEKELQE
CEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LDNRISELVQEGEYQKAAELKTKFEKIRKEYEARKAEWFKSDNAQNNEVTEEIIASIVEQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
WTGIPAGKLLESERDKLKNLEKLIHERFIDQEEAVSAVAHTIRRARAGLKAPNRPWGSFL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FLGPTGVGKTELAKTLANILFGSEDAMIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQL
EECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TEAVRRRPYSVILLDEVEKAHPDVHNVLLQVMDDGRLTDGKGKTVNFSNTILIMTSNVGS
HHHHHHCCCEEEEECCHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCCCC
EEMTKGKFDENTTALVEQKLKSAFKPEFLNRLDAIVFFKPLSKEHLREIVKLRLKEVEEK
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSEQGISMRYTDKAVDYLADRGYDPVYGARPIRRLVERDVEGQIADMIISGELSENDVVV
HHHCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHEECCCCCCCEEE
VGADDFGIKIYKEE
EECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]