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The map label for this gene is rbsB [H]

Identifier: 239617002

GI number: 239617002

Start: 649402

End: 650280

Strand: Reverse

Name: rbsB [H]

Synonym: Kole_0602

Alternate gene names: 239617002

Gene position: 650280-649402 (Counterclockwise)

Preceding gene: 239617003

Following gene: 239617001

Centisome position: 28.25

GC content: 45.96

Gene sequence:

>879_bases
ATGAAAAAGGTACTGGTGTTATTCAGTTTATTGCTTTTGGTGTCAGTCTTCGCATTGGGAGCAACTTACAGAATAGGTCT
GTCTCTTTCGACACTCAACAACCCGTTCTTTGTTACGTTGAGAGACGGTGCGCTGGACATGGCAGGTAAATTGGGGGTAG
AAGTTATCGTTGCAGATGCTCAGGACAACCCGGCGAAACAGCTCAGCGATATCGAAGACTTCATTCAACAACGTCTAGAT
CTGATAATCATCAACCCAACTGACAGTGATGCAATCGTAACGGCAGTTGAGGAAGCAAACGATGCCGGCATTCCTGTGAT
AACAGTTGACAGAGCTTCCAATGGTGGAAAGGTTGTTTGCCACATCGCTTCTGATAACGTTGCCGGTGGAAGGATGGCCG
GTGAATTTGTGGCGAAACTTCTTGGTGGAAAAGGTAAGGTCGTTGAACTGGAAGGTATAGTTGGAACTTCCGCTGCGAGA
GATCGCGGAAAAGGTTTTGAAGAAGCTATAGCAAAATACCCCGGGATAAAGCTGGTAGCAAAACAGACCGCTAACTTCAA
CAGAGCGGAAGGGTTAATAGTTATGGAAAACATTCTTCAGGCACATCCAGATATTGACGCCGTTTTTGCCCAGAACGATG
AAATGGCACTTGGAGCGATTGAGGCGATCAAAGCAGCCGGACTGCTTGGAAAAATCTATGTAGTTGGTTTTGATGCCATC
GATGATGCTGTTGAAGCAGTCAAAAAAGGCGAAATGGCTGCGACCATTGCCCAGCAACCTTACAAGATGGGAGAGCTTGC
AGTTCTCAAGGCGTTCGAGTACCTCACAACGGGCTCTATTTATATCCCTGTTGATCTAAAACTTGTTGTGAATGAATAA

Upstream 100 bases:

>100_bases
CTGTAAAAGGTGCAGTAATACTGCTCGCCGTTATGGCTGAACGTGCGGGTCAGAAATAAAAGGGGCTATATGCCCCTATT
CTGATAGGGGAGGGTTAAGT

Downstream 100 bases:

>100_bases
TGGAAAAGGAGGGGGAATGTCCCCCCTCCGTTTTTTGTGGAGGGATTCGTATGATAGCAGTAGTCGGAAGCAACAATGTT
GATGTAGTTCTCACGGTTGA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKKVLVLFSLLLLVSVFALGATYRIGLSLSTLNNPFFVTLRDGALDMAGKLGVEVIVADAQDNPAKQLSDIEDFIQQRLD
LIIINPTDSDAIVTAVEEANDAGIPVITVDRASNGGKVVCHIASDNVAGGRMAGEFVAKLLGGKGKVVELEGIVGTSAAR
DRGKGFEEAIAKYPGIKLVAKQTANFNRAEGLIVMENILQAHPDIDAVFAQNDEMALGAIEAIKAAGLLGKIYVVGFDAI
DDAVEAVKKGEMAATIAQQPYKMGELAVLKAFEYLTTGSIYIPVDLKLVVNE

Sequences:

>Translated_292_residues
MKKVLVLFSLLLLVSVFALGATYRIGLSLSTLNNPFFVTLRDGALDMAGKLGVEVIVADAQDNPAKQLSDIEDFIQQRLD
LIIINPTDSDAIVTAVEEANDAGIPVITVDRASNGGKVVCHIASDNVAGGRMAGEFVAKLLGGKGKVVELEGIVGTSAAR
DRGKGFEEAIAKYPGIKLVAKQTANFNRAEGLIVMENILQAHPDIDAVFAQNDEMALGAIEAIKAAGLLGKIYVVGFDAI
DDAVEAVKKGEMAATIAQQPYKMGELAVLKAFEYLTTGSIYIPVDLKLVVNE
>Mature_292_residues
MKKVLVLFSLLLLVSVFALGATYRIGLSLSTLNNPFFVTLRDGALDMAGKLGVEVIVADAQDNPAKQLSDIEDFIQQRLD
LIIINPTDSDAIVTAVEEANDAGIPVITVDRASNGGKVVCHIASDNVAGGRMAGEFVAKLLGGKGKVVELEGIVGTSAAR
DRGKGFEEAIAKYPGIKLVAKQTANFNRAEGLIVMENILQAHPDIDAVFAQNDEMALGAIEAIKAAGLLGKIYVVGFDAI
DDAVEAVKKGEMAATIAQQPYKMGELAVLKAFEYLTTGSIYIPVDLKLVVNE

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=296, Percent_Identity=50.3378378378378, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI1790526, Length=300, Percent_Identity=34.6666666666667, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790674, Length=255, Percent_Identity=34.5098039215686, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1789990, Length=256, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788898, Length=303, Percent_Identity=24.4224422442244, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788473, Length=278, Percent_Identity=27.3381294964029, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 30902; Mature: 30902

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLVLFSLLLLVSVFALGATYRIGLSLSTLNNPFFVTLRDGALDMAGKLGVEVIVADA
CCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEECCCCCHHCCCCEEEEEEEC
QDNPAKQLSDIEDFIQQRLDLIIINPTDSDAIVTAVEEANDAGIPVITVDRASNGGKVVC
CCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEE
HIASDNVAGGRMAGEFVAKLLGGKGKVVELEGIVGTSAARDRGKGFEEAIAKYPGIKLVA
EEECCCCCCCHHHHHHHHHHHCCCCCEEEEECEECCCCCHHCCCCHHHHHHHCCCEEEEE
KQTANFNRAEGLIVMENILQAHPDIDAVFAQNDEMALGAIEAIKAAGLLGKIYVVGFDAI
ECCCCCCCCCCEEEEHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEEEHHH
DDAVEAVKKGEMAATIAQQPYKMGELAVLKAFEYLTTGSIYIPVDLKLVVNE
HHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEECC
>Mature Secondary Structure
MKKVLVLFSLLLLVSVFALGATYRIGLSLSTLNNPFFVTLRDGALDMAGKLGVEVIVADA
CCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEECCCCCHHCCCCEEEEEEEC
QDNPAKQLSDIEDFIQQRLDLIIINPTDSDAIVTAVEEANDAGIPVITVDRASNGGKVVC
CCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEE
HIASDNVAGGRMAGEFVAKLLGGKGKVVELEGIVGTSAARDRGKGFEEAIAKYPGIKLVA
EEECCCCCCCHHHHHHHHHHHCCCCCEEEEECEECCCCCHHCCCCHHHHHHHCCCEEEEE
KQTANFNRAEGLIVMENILQAHPDIDAVFAQNDEMALGAIEAIKAAGLLGKIYVVGFDAI
ECCCCCCCCCCEEEEHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEEEHHH
DDAVEAVKKGEMAATIAQQPYKMGELAVLKAFEYLTTGSIYIPVDLKLVVNE
HHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]