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The map label for this gene is murE [H]

Identifier: 239616915

GI number: 239616915

Start: 556984

End: 558450

Strand: Reverse

Name: murE [H]

Synonym: Kole_0511

Alternate gene names: 239616915

Gene position: 558450-556984 (Counterclockwise)

Preceding gene: 239616916

Following gene: 239616914

Centisome position: 24.26

GC content: 40.01

Gene sequence:

>1467_bases
ATGGAGATGGTTCTAAGAGAACTGATAAAAGATTTGAAAGAAAAAAGGGTTGAAGGTAGGCAGGACTTAGTAATTCGCGG
CATTGAATGTGATTCGAGAAAAGTGGAAGAAGGTTTTTTATTTGTTTGTATTCCGGGTTTTAATTTTGATGGACACAAAT
TCATCCCCGAGGCAATAAAGAGAGGTTCCCGGGTAGTGGTGGTTGAGAAAGACGTTAAAACAGCTAAAGATGTCACTGTT
ATTAAAGTTCCTAACACAAGATATGCTCTAGCGGTCTTGGCGAACAGGTTTTATGGATATCCGTCAAAAAAACTGCAGGT
AGTAGGAATTGCCGGTACTAATGGCAAGACAACCACCACTTATCTGGTAAGGGATGTATTAAAAAAGGCGGGAAAGAAGG
TAGCTCTATTTGGAACAATTGAGTACGACTTAGGGAACAGGACATTGCCGGCAAGTACGACCACTCCACAAAGCCTGGAG
CTTCAGGCAATGTTAAACGAACTGGTAAACAAAGAGTTTGAATACGTGGTTATGGAGGTATCTACCCACGCCACGGTGCT
GGACAGGGTAACTGGTTGTGAATTTGATATAGGCGTATTTACCAACATAAGTAGAGATCACATGGATTTTCATAAAACAA
TGGAGAACTATTTGGAAGCAGAGATAAAGTTCTTCAGGCTTTTAAGCCGGACGGGAAGAAGAGCAGTTATAAATATCGAT
GATCCTTACGCGGAGCATATTATAAAAAACACAAGGACAGAAATTCTGACTTATGGAATAAATGATAAAAATGCAGACAT
AAGAGCTTTTGATATAAAAGTCTCGAAAACAGGGTCAAGATTTAATGTTATCACCCCTTTAGGAGAAACGGTATTCTACC
TCCGATTACCAGGTAGATACAACGTGTATAACGCCCTTGCTGCTGTTGGGGTGGGAATAAGTGAAGGACTGAACCTTAAT
GAAATTAGAGAAGCTATCGCTCATGCAAGTGTTCCGGGAAGGTTTGAACGAATTGACTGTGGGCAATCTTTTGATGTGAT
TGTTGACTATGCCCATACTCCTGATGCACTTCAAAAAGCTCTTGAAACCGCAAGAGGACTTTCTGAAGGAAGAATAATAG
TTGTATTTGGTTGTGGAGATGATAAGGACAGGCGGCCCTTAATGGGTGTGGTGGCAGCAAGAATGAGTGATATCGCAATT
CTTACCTCTGACAACTCAAGAAATGAAGAGCCGTCAGAAATTATAAGTGATATTGAATCTGGAATCAAGAAAGTCGATAA
TTCTTTCCAATATCTGGTTATCGAAGATCGCTTTGAGGCAATAACACAAGCATTGGAAATGGCAAAAGAGGGAGATTTGG
TGCTTATTGCAGGAAAAGGGCATGAAGAGTATCAAATTATCAAAGACAGAAAAATTCCCTTTAATGATAGAAAAGTGGCG
GAGAGGATACTGAAAAATGGATTATAA

Upstream 100 bases:

>100_bases
GTGATATTGTTTTGATCAAAGGCTCGCGTGGAATGAAGATGGAAGAAATAGTGGAGAGATTGAGAAAAAGGCTTGGAGTC
TTCTAGGGGAGGACCAAAAC

Downstream 100 bases:

>100_bases
AGAAGCTTTAGATTATCTATATAGTTTGAGAAGATTTGGCATTAAGCCAGGAGTTAGAAGAATAAAAATGCTTCTTGAAC
TTTTGGGGAATCCGCAGGAT

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MEMVLRELIKDLKEKRVEGRQDLVIRGIECDSRKVEEGFLFVCIPGFNFDGHKFIPEAIKRGSRVVVVEKDVKTAKDVTV
IKVPNTRYALAVLANRFYGYPSKKLQVVGIAGTNGKTTTTYLVRDVLKKAGKKVALFGTIEYDLGNRTLPASTTTPQSLE
LQAMLNELVNKEFEYVVMEVSTHATVLDRVTGCEFDIGVFTNISRDHMDFHKTMENYLEAEIKFFRLLSRTGRRAVINID
DPYAEHIIKNTRTEILTYGINDKNADIRAFDIKVSKTGSRFNVITPLGETVFYLRLPGRYNVYNALAAVGVGISEGLNLN
EIREAIAHASVPGRFERIDCGQSFDVIVDYAHTPDALQKALETARGLSEGRIIVVFGCGDDKDRRPLMGVVAARMSDIAI
LTSDNSRNEEPSEIISDIESGIKKVDNSFQYLVIEDRFEAITQALEMAKEGDLVLIAGKGHEEYQIIKDRKIPFNDRKVA
ERILKNGL

Sequences:

>Translated_488_residues
MEMVLRELIKDLKEKRVEGRQDLVIRGIECDSRKVEEGFLFVCIPGFNFDGHKFIPEAIKRGSRVVVVEKDVKTAKDVTV
IKVPNTRYALAVLANRFYGYPSKKLQVVGIAGTNGKTTTTYLVRDVLKKAGKKVALFGTIEYDLGNRTLPASTTTPQSLE
LQAMLNELVNKEFEYVVMEVSTHATVLDRVTGCEFDIGVFTNISRDHMDFHKTMENYLEAEIKFFRLLSRTGRRAVINID
DPYAEHIIKNTRTEILTYGINDKNADIRAFDIKVSKTGSRFNVITPLGETVFYLRLPGRYNVYNALAAVGVGISEGLNLN
EIREAIAHASVPGRFERIDCGQSFDVIVDYAHTPDALQKALETARGLSEGRIIVVFGCGDDKDRRPLMGVVAARMSDIAI
LTSDNSRNEEPSEIISDIESGIKKVDNSFQYLVIEDRFEAITQALEMAKEGDLVLIAGKGHEEYQIIKDRKIPFNDRKVA
ERILKNGL
>Mature_488_residues
MEMVLRELIKDLKEKRVEGRQDLVIRGIECDSRKVEEGFLFVCIPGFNFDGHKFIPEAIKRGSRVVVVEKDVKTAKDVTV
IKVPNTRYALAVLANRFYGYPSKKLQVVGIAGTNGKTTTTYLVRDVLKKAGKKVALFGTIEYDLGNRTLPASTTTPQSLE
LQAMLNELVNKEFEYVVMEVSTHATVLDRVTGCEFDIGVFTNISRDHMDFHKTMENYLEAEIKFFRLLSRTGRRAVINID
DPYAEHIIKNTRTEILTYGINDKNADIRAFDIKVSKTGSRFNVITPLGETVFYLRLPGRYNVYNALAAVGVGISEGLNLN
EIREAIAHASVPGRFERIDCGQSFDVIVDYAHTPDALQKALETARGLSEGRIIVVFGCGDDKDRRPLMGVVAARMSDIAI
LTSDNSRNEEPSEIISDIESGIKKVDNSFQYLVIEDRFEAITQALEMAKEGDLVLIAGKGHEEYQIIKDRKIPFNDRKVA
ERILKNGL

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=491, Percent_Identity=35.234215885947, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1786274, Length=374, Percent_Identity=24.8663101604278, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54767; Mature: 54767

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMVLRELIKDLKEKRVEGRQDLVIRGIECDSRKVEEGFLFVCIPGFNFDGHKFIPEAIK
CHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
RGSRVVVVEKDVKTAKDVTVIKVPNTRYALAVLANRFYGYPSKKLQVVGIAGTNGKTTTT
CCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHH
YLVRDVLKKAGKKVALFGTIEYDLGNRTLPASTTTPQSLELQAMLNELVNKEFEYVVMEV
HHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEE
STHATVLDRVTGCEFDIGVFTNISRDHMDFHKTMENYLEAEIKFFRLLSRTGRRAVINID
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
DPYAEHIIKNTRTEILTYGINDKNADIRAFDIKVSKTGSRFNVITPLGETVFYLRLPGRY
CHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEECCCCEEEEEECCCCEEEEEECCCCC
NVYNALAAVGVGISEGLNLNEIREAIAHASVPGRFERIDCGQSFDVIVDYAHTPDALQKA
CHHHHHHHHCCCHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCHHHHHHH
LETARGLSEGRIIVVFGCGDDKDRRPLMGVVAARMSDIAILTSDNSRNEEPSEIISDIES
HHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHH
GIKKVDNSFQYLVIEDRFEAITQALEMAKEGDLVLIAGKGHEEYQIIKDRKIPFNDRKVA
HHHHHCCCEEEEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCHHHHH
ERILKNGL
HHHHHCCC
>Mature Secondary Structure
MEMVLRELIKDLKEKRVEGRQDLVIRGIECDSRKVEEGFLFVCIPGFNFDGHKFIPEAIK
CHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
RGSRVVVVEKDVKTAKDVTVIKVPNTRYALAVLANRFYGYPSKKLQVVGIAGTNGKTTTT
CCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHH
YLVRDVLKKAGKKVALFGTIEYDLGNRTLPASTTTPQSLELQAMLNELVNKEFEYVVMEV
HHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEE
STHATVLDRVTGCEFDIGVFTNISRDHMDFHKTMENYLEAEIKFFRLLSRTGRRAVINID
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
DPYAEHIIKNTRTEILTYGINDKNADIRAFDIKVSKTGSRFNVITPLGETVFYLRLPGRY
CHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEECCCCEEEEEECCCCEEEEEECCCCC
NVYNALAAVGVGISEGLNLNEIREAIAHASVPGRFERIDCGQSFDVIVDYAHTPDALQKA
CHHHHHHHHCCCHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCHHHHHHH
LETARGLSEGRIIVVFGCGDDKDRRPLMGVVAARMSDIAILTSDNSRNEEPSEIISDIES
HHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHH
GIKKVDNSFQYLVIEDRFEAITQALEMAKEGDLVLIAGKGHEEYQIIKDRKIPFNDRKVA
HHHHHCCCEEEEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCHHHHH
ERILKNGL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA