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The map label for this gene is murF [H]

Identifier: 239616916

GI number: 239616916

Start: 558465

End: 559871

Strand: Reverse

Name: murF [H]

Synonym: Kole_0512

Alternate gene names: 239616916

Gene position: 559871-558465 (Counterclockwise)

Preceding gene: 239616917

Following gene: 239616915

Centisome position: 24.32

GC content: 38.24

Gene sequence:

>1407_bases
ATGGAACCGATATCTTTGTCTAAAATTATTGAAGCTACAGGTGGGACTTTACTCTCGGGAACTCCTGATGTTGAGATTTT
TGGCATTTCCACCGACACTCGAACAATACACAAAGGGGATTTATTTGTTGCGCTGGAGGGGGAAAGATTTGATGGGCATT
ATTTTATAGACGAAGCCTTCAAAAAAGGAGCGATAGGCGCAATTGTGTCAAGAGAGGTTGAAAGCAAAAATATAATTATA
AAGGTGAGAGATACACTTAAAACTCTTGGAGACATAGCTAAGATTTATAGAGAAGAGTTCGACATCCCTGTTATAGCGGT
TACTGGTTCTAATGGCAAAACAACCACAAAAGAAATGCTAGGGTATATCTTAACCAGGGAGTTCAAAACTGTAAAGGCAA
AGGGAAGCTACAATAACTTTGTGGGAGTACCATTGACCTTGTTGGAAATGGACAGGCATACGCAGCTGGCTGTTCTAGAA
ATGGAAACAAATTTATTGGGAGGTATAAAAAGGCTTTGTGAGATAGCTAAACCTTCAGTTGGTGTCGTTACTAATATTGG
TGATACTCACCTGCAGTTTTTGAAAACAAGGGAAGGGGTATATCAGGAGAAATCAGAACTTGTGGAATCTTTGCCTGAGG
ATGGGGTAGCGGTTTTGAATGCCGATGATCCATACGTATTAAAAATGAAGGAGATATCTAGAACAAAGCGGATAGTTACT
TTTGGAGTAGAAAATAAATCCGAATTTTCAGCTTCTAATATAAGAGTTGGGGATACGTTTCTCGAATTTACCCTAAACAA
TAATTACAGAGTTAAACTAAATACTATTTCCTATTGTAATGTTTACAATGGATTAGCAGCAATCGCTGTTTCCCATGTGG
TTTTTGGTTTGAGTTTAGAGAAAGTGATAGATATTCTGAAAGACTTTAAATTTCCCTCTCTGCGAATGGAATTAATCGAA
ATTCCTCTTATAGATAGAGCGACTTCGAATGAAGAAAGTGTAAGGATTATTAATGATTGTTATAACGCTAATCCTCAATC
AATGCAAGAAGCTCTGGTTGCTGTTGGAAAGATGAATTCTTATACTAATAAGATAGCAATTCTGGGTGAAATGCTGGAAT
TGGGGGAAAAAGCTCCAGAATTCCATTATCAGATAGGTAAGATATGTGCGGATTGTGGATTTGACATGTTGATAACCGTA
GGAGATCTTGCGCAATATATGGCTCATGGAGCTGAAGATGAAGGTATGCCCAGGGAAAATATATTTGTTTCTGTCAATAA
TGTGGAAGCGTCAGATCGTCTTTTGAGAATCCTGAGACCGGGTGATATTGTTTTGATCAAAGGCTCGCGTGGAATGAAGA
TGGAAGAAATAGTGGAGAGATTGAGAAAAAGGCTTGGAGTCTTCTAG

Upstream 100 bases:

>100_bases
TAAATTAGATGTTGTCATAGGTAATATTATTGAATTAGAAAATACCAGGGAACTGTGAACAAAAGCTTTCTATCTTCATG
TTTTTTCGGAGGTAACTTTC

Downstream 100 bases:

>100_bases
GGGAGGACCAAAACATGGAGATGGTTCTAAGAGAACTGATAAAAGATTTGAAAGAAAAAAGGGTTGAAGGTAGGCAGGAC
TTAGTAATTCGCGGCATTGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MEPISLSKIIEATGGTLLSGTPDVEIFGISTDTRTIHKGDLFVALEGERFDGHYFIDEAFKKGAIGAIVSREVESKNIII
KVRDTLKTLGDIAKIYREEFDIPVIAVTGSNGKTTTKEMLGYILTREFKTVKAKGSYNNFVGVPLTLLEMDRHTQLAVLE
METNLLGGIKRLCEIAKPSVGVVTNIGDTHLQFLKTREGVYQEKSELVESLPEDGVAVLNADDPYVLKMKEISRTKRIVT
FGVENKSEFSASNIRVGDTFLEFTLNNNYRVKLNTISYCNVYNGLAAIAVSHVVFGLSLEKVIDILKDFKFPSLRMELIE
IPLIDRATSNEESVRIINDCYNANPQSMQEALVAVGKMNSYTNKIAILGEMLELGEKAPEFHYQIGKICADCGFDMLITV
GDLAQYMAHGAEDEGMPRENIFVSVNNVEASDRLLRILRPGDIVLIKGSRGMKMEEIVERLRKRLGVF

Sequences:

>Translated_468_residues
MEPISLSKIIEATGGTLLSGTPDVEIFGISTDTRTIHKGDLFVALEGERFDGHYFIDEAFKKGAIGAIVSREVESKNIII
KVRDTLKTLGDIAKIYREEFDIPVIAVTGSNGKTTTKEMLGYILTREFKTVKAKGSYNNFVGVPLTLLEMDRHTQLAVLE
METNLLGGIKRLCEIAKPSVGVVTNIGDTHLQFLKTREGVYQEKSELVESLPEDGVAVLNADDPYVLKMKEISRTKRIVT
FGVENKSEFSASNIRVGDTFLEFTLNNNYRVKLNTISYCNVYNGLAAIAVSHVVFGLSLEKVIDILKDFKFPSLRMELIE
IPLIDRATSNEESVRIINDCYNANPQSMQEALVAVGKMNSYTNKIAILGEMLELGEKAPEFHYQIGKICADCGFDMLITV
GDLAQYMAHGAEDEGMPRENIFVSVNNVEASDRLLRILRPGDIVLIKGSRGMKMEEIVERLRKRLGVF
>Mature_468_residues
MEPISLSKIIEATGGTLLSGTPDVEIFGISTDTRTIHKGDLFVALEGERFDGHYFIDEAFKKGAIGAIVSREVESKNIII
KVRDTLKTLGDIAKIYREEFDIPVIAVTGSNGKTTTKEMLGYILTREFKTVKAKGSYNNFVGVPLTLLEMDRHTQLAVLE
METNLLGGIKRLCEIAKPSVGVVTNIGDTHLQFLKTREGVYQEKSELVESLPEDGVAVLNADDPYVLKMKEISRTKRIVT
FGVENKSEFSASNIRVGDTFLEFTLNNNYRVKLNTISYCNVYNGLAAIAVSHVVFGLSLEKVIDILKDFKFPSLRMELIE
IPLIDRATSNEESVRIINDCYNANPQSMQEALVAVGKMNSYTNKIAILGEMLELGEKAPEFHYQIGKICADCGFDMLITV
GDLAQYMAHGAEDEGMPRENIFVSVNNVEASDRLLRILRPGDIVLIKGSRGMKMEEIVERLRKRLGVF

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=469, Percent_Identity=31.3432835820896, Blast_Score=200, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 52066; Mature: 52066

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPISLSKIIEATGGTLLSGTPDVEIFGISTDTRTIHKGDLFVALEGERFDGHYFIDEAF
CCCCCHHHHHHHCCCEEECCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCEEEHHHHH
KKGAIGAIVSREVESKNIIIKVRDTLKTLGDIAKIYREEFDIPVIAVTGSNGKTTTKEML
CCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
GYILTREFKTVKAKGSYNNFVGVPLTLLEMDRHTQLAVLEMETNLLGGIKRLCEIAKPSV
HHHHHHHHHEEEECCCCCCCCCCCEEEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCC
GVVTNIGDTHLQFLKTREGVYQEKSELVESLPEDGVAVLNADDPYVLKMKEISRTKRIVT
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHCCCCEEEE
FGVENKSEFSASNIRVGDTFLEFTLNNNYRVKLNTISYCNVYNGLAAIAVSHVVFGLSLE
EECCCCCCCCCCCEEECCEEEEEEECCCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCHH
KVIDILKDFKFPSLRMELIEIPLIDRATSNEESVRIINDCYNANPQSMQEALVAVGKMNS
HHHHHHHHCCCCCHHHHHEECCCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCC
YTNKIAILGEMLELGEKAPEFHYQIGKICADCGFDMLITVGDLAQYMAHGAEDEGMPREN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEEHHHHHHHHHCCCCCCCCCCCC
IFVSVNNVEASDRLLRILRPGDIVLIKGSRGMKMEEIVERLRKRLGVF
EEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPISLSKIIEATGGTLLSGTPDVEIFGISTDTRTIHKGDLFVALEGERFDGHYFIDEAF
CCCCCHHHHHHHCCCEEECCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCEEEHHHHH
KKGAIGAIVSREVESKNIIIKVRDTLKTLGDIAKIYREEFDIPVIAVTGSNGKTTTKEML
CCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
GYILTREFKTVKAKGSYNNFVGVPLTLLEMDRHTQLAVLEMETNLLGGIKRLCEIAKPSV
HHHHHHHHHEEEECCCCCCCCCCCEEEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCC
GVVTNIGDTHLQFLKTREGVYQEKSELVESLPEDGVAVLNADDPYVLKMKEISRTKRIVT
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHCCCCEEEE
FGVENKSEFSASNIRVGDTFLEFTLNNNYRVKLNTISYCNVYNGLAAIAVSHVVFGLSLE
EECCCCCCCCCCCEEECCEEEEEEECCCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCHH
KVIDILKDFKFPSLRMELIEIPLIDRATSNEESVRIINDCYNANPQSMQEALVAVGKMNS
HHHHHHHHCCCCCHHHHHEECCCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCC
YTNKIAILGEMLELGEKAPEFHYQIGKICADCGFDMLITVGDLAQYMAHGAEDEGMPREN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEEHHHHHHHHHCCCCCCCCCCCC
IFVSVNNVEASDRLLRILRPGDIVLIKGSRGMKMEEIVERLRKRLGVF
EEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]