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The map label for this gene is folC [H]

Identifier: 239616914

GI number: 239616914

Start: 555708

End: 556994

Strand: Reverse

Name: folC [H]

Synonym: Kole_0510

Alternate gene names: 239616914

Gene position: 556994-555708 (Counterclockwise)

Preceding gene: 239616915

Following gene: 239616913

Centisome position: 24.19

GC content: 37.3

Gene sequence:

>1287_bases
ATGGATTATAAAGAAGCTTTAGATTATCTATATAGTTTGAGAAGATTTGGCATTAAGCCAGGAGTTAGAAGAATAAAAAT
GCTTCTTGAACTTTTGGGGAATCCGCAGGATTCCCTCAATGTTGTTCATGTTGCGGGTACAAATGGCAAAGGTTCTACCG
TTGCCTTTATAAGTTCTATACTGAAAGAAGCAGGATATAGAGTAGGAACATATATTTCACCGCATTTGATTGATTTTACT
GAAAGAATGACCATAAATGGACTTCAAATAAAAGAAGAGGTTGTGGTAGAGATTTTGAATAAAATCAGACCACATATTAC
AGAGGTAGCCAATTCTAAATTCGGGTCTCCTACTTTTTTTGAAGTTGTGACTGCGATGGCTTTTGTTTATTTTAAACAGC
AAAAAGTAGACTTTGCTGTTTTAGAAGTAGGATTGGGTGGAGAGAATGACGCTACAAATGTAGTTAAGCCTCTGGTTTCT
GTCATAACAAATAGTGACTATGACCATATGGATATTCTGGGAAACGATATAACCTCTATCGCGAAAGAAGATGCCGGGAT
TATCAAAGAAAATGGGTTCGTGGTTACAGCAGCAGAAGGGGAAGCGCTCGATGTTATTAAGAAAATAGGTGAGGAAAAGA
GGGCAAGGATTTATCAGATAGGAAGGGATATAAAGTACGAATTCGTGAATTCAGATCTCAAATGGCAAAGATTCAATGTA
AAGGGAATCTTTTCTGAGTCCGACAACTTAAAGATACCACTATTAGGAAAATACCAGCTGATAAATGCCAGTTGTGCCGT
TGGTGCGATAGAGATTCTTAGATTTTATGATATTGAGATCAGTGATGAAAGTATCAGAAAAGGTTTGGAAAACACATTCT
GGCCTGGCAGATTTGAAATAGTACAGGATGCACCCATGGTGGTTTTAGATGGTGCTCACAATCATCTGGCTGCAAAAAGA
CTAAAAGAAGCTCTTATGGAGGTTCTTGAAAAGAAGGATAAGCTGATATTGGTAATCGGGATTATGAAGGACAAAGAAGT
AGAAAAAATCGTAAAAGAACTTGCTCCACTGGCATATAAAGTGGTGGCTACATCTCCAAAAACCCCGAGAGCTACTACAT
CTCATGATTTGCGTGAGATTGTAGTTAAATTTAACAAAAACATCAGTGTTGTTGAAGATGTGGAAACTGCGCTAGAATTA
GCTTTAAGTGAAGCAAAGGAAAATGATATAGTTTGTGTTACCGGTTCTCTGTATACTGTAGGAGAGGCGAGAAAGTTTTT
ATTATGA

Upstream 100 bases:

>100_bases
TTGGTGCTTATTGCAGGAAAAGGGCATGAAGAGTATCAAATTATCAAAGACAGAAAAATTCCCTTTAATGATAGAAAAGT
GGCGGAGAGGATACTGAAAA

Downstream 100 bases:

>100_bases
TGAGTATGCTTTATTGGTGCTTCACCAATTAACCTTTAGGGGTGGAAGTATGGCTAAACACATTGGAATAGTTGCATGCA
GTGCTGAGGGAGCAGCTCTT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MDYKEALDYLYSLRRFGIKPGVRRIKMLLELLGNPQDSLNVVHVAGTNGKGSTVAFISSILKEAGYRVGTYISPHLIDFT
ERMTINGLQIKEEVVVEILNKIRPHITEVANSKFGSPTFFEVVTAMAFVYFKQQKVDFAVLEVGLGGENDATNVVKPLVS
VITNSDYDHMDILGNDITSIAKEDAGIIKENGFVVTAAEGEALDVIKKIGEEKRARIYQIGRDIKYEFVNSDLKWQRFNV
KGIFSESDNLKIPLLGKYQLINASCAVGAIEILRFYDIEISDESIRKGLENTFWPGRFEIVQDAPMVVLDGAHNHLAAKR
LKEALMEVLEKKDKLILVIGIMKDKEVEKIVKELAPLAYKVVATSPKTPRATTSHDLREIVVKFNKNISVVEDVETALEL
ALSEAKENDIVCVTGSLYTVGEARKFLL

Sequences:

>Translated_428_residues
MDYKEALDYLYSLRRFGIKPGVRRIKMLLELLGNPQDSLNVVHVAGTNGKGSTVAFISSILKEAGYRVGTYISPHLIDFT
ERMTINGLQIKEEVVVEILNKIRPHITEVANSKFGSPTFFEVVTAMAFVYFKQQKVDFAVLEVGLGGENDATNVVKPLVS
VITNSDYDHMDILGNDITSIAKEDAGIIKENGFVVTAAEGEALDVIKKIGEEKRARIYQIGRDIKYEFVNSDLKWQRFNV
KGIFSESDNLKIPLLGKYQLINASCAVGAIEILRFYDIEISDESIRKGLENTFWPGRFEIVQDAPMVVLDGAHNHLAAKR
LKEALMEVLEKKDKLILVIGIMKDKEVEKIVKELAPLAYKVVATSPKTPRATTSHDLREIVVKFNKNISVVEDVETALEL
ALSEAKENDIVCVTGSLYTVGEARKFLL
>Mature_428_residues
MDYKEALDYLYSLRRFGIKPGVRRIKMLLELLGNPQDSLNVVHVAGTNGKGSTVAFISSILKEAGYRVGTYISPHLIDFT
ERMTINGLQIKEEVVVEILNKIRPHITEVANSKFGSPTFFEVVTAMAFVYFKQQKVDFAVLEVGLGGENDATNVVKPLVS
VITNSDYDHMDILGNDITSIAKEDAGIIKENGFVVTAAEGEALDVIKKIGEEKRARIYQIGRDIKYEFVNSDLKWQRFNV
KGIFSESDNLKIPLLGKYQLINASCAVGAIEILRFYDIEISDESIRKGLENTFWPGRFEIVQDAPMVVLDGAHNHLAAKR
LKEALMEVLEKKDKLILVIGIMKDKEVEKIVKELAPLAYKVVATSPKTPRATTSHDLREIVVKFNKNISVVEDVETALEL
ALSEAKENDIVCVTGSLYTVGEARKFLL

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=365, Percent_Identity=28.2191780821918, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI66932984, Length=365, Percent_Identity=28.2191780821918, Blast_Score=155, Evalue=7e-38,
Organism=Escherichia coli, GI1788654, Length=416, Percent_Identity=33.4134615384615, Blast_Score=205, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17553150, Length=454, Percent_Identity=29.5154185022026, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17553148, Length=454, Percent_Identity=29.5154185022026, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71984923, Length=454, Percent_Identity=29.5154185022026, Blast_Score=158, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6323760, Length=429, Percent_Identity=32.4009324009324, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6324815, Length=440, Percent_Identity=30.9090909090909, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322718, Length=283, Percent_Identity=25.0883392226148, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24641571, Length=282, Percent_Identity=31.9148936170213, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24581568, Length=183, Percent_Identity=34.4262295081967, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47771; Mature: 47771

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKEALDYLYSLRRFGIKPGVRRIKMLLELLGNPQDSLNVVHVAGTNGKGSTVAFISSI
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
LKEAGYRVGTYISPHLIDFTERMTINGLQIKEEVVVEILNKIRPHITEVANSKFGSPTFF
HHHCCCEECCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EVVTAMAFVYFKQQKVDFAVLEVGLGGENDATNVVKPLVSVITNSDYDHMDILGNDITSI
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHHHH
AKEDAGIIKENGFVVTAAEGEALDVIKKIGEEKRARIYQIGRDIKYEFVNSDLKWQRFNV
HHHCCCEEECCCEEEEECCCCHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCEEEEEE
KGIFSESDNLKIPLLGKYQLINASCAVGAIEILRFYDIEISDESIRKGLENTFWPGRFEI
EEEECCCCCEEEEEECCEEEECCHHHHHHHHHHHHCCEEECHHHHHCCHHHCCCCCCEEE
VQDAPMVVLDGAHNHLAAKRLKEALMEVLEKKDKLILVIGIMKDKEVEKIVKELAPLAYK
ECCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHEE
VVATSPKTPRATTSHDLREIVVKFNKNISVVEDVETALELALSEAKENDIVCVTGSLYTV
EEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCEEEC
GEARKFLL
CCHHHHCC
>Mature Secondary Structure
MDYKEALDYLYSLRRFGIKPGVRRIKMLLELLGNPQDSLNVVHVAGTNGKGSTVAFISSI
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
LKEAGYRVGTYISPHLIDFTERMTINGLQIKEEVVVEILNKIRPHITEVANSKFGSPTFF
HHHCCCEECCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EVVTAMAFVYFKQQKVDFAVLEVGLGGENDATNVVKPLVSVITNSDYDHMDILGNDITSI
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHHHH
AKEDAGIIKENGFVVTAAEGEALDVIKKIGEEKRARIYQIGRDIKYEFVNSDLKWQRFNV
HHHCCCEEECCCEEEEECCCCHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCEEEEEE
KGIFSESDNLKIPLLGKYQLINASCAVGAIEILRFYDIEISDESIRKGLENTFWPGRFEI
EEEECCCCCEEEEEECCEEEECCHHHHHHHHHHHHCCEEECHHHHHCCHHHCCCCCCEEE
VQDAPMVVLDGAHNHLAAKRLKEALMEVLEKKDKLILVIGIMKDKEVEKIVKELAPLAYK
ECCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHEE
VVATSPKTPRATTSHDLREIVVKFNKNISVVEDVETALELALSEAKENDIVCVTGSLYTV
EEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCEEEC
GEARKFLL
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]