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The map label for this gene is nuc [H]

Identifier: 239616544

GI number: 239616544

Start: 153869

End: 154708

Strand: Direct

Name: nuc [H]

Synonym: Kole_0136

Alternate gene names: 239616544

Gene position: 153869-154708 (Clockwise)

Preceding gene: 239616543

Following gene: 239616545

Centisome position: 6.68

GC content: 39.29

Gene sequence:

>840_bases
ATGAGTTCCCACAGATCTGTGATAAAGCAATCATGGCTGTATGTATCGTTAGTAATTTTAGTTTCAATCTCTGCGCTGTT
CCTCACCTCGTGTCCCGGGTGGTTTACTTCATCATATTATGTAACAGAAATAATAGATGGAGACACTATTAAAGTTCTAT
ACAATGGCGAAGAAGTCAAAGTCCGATACATAGGCATAGATACCCCGGAAACTCATTCAGATTACCAGGATTCAAAGCCA
AACGGAGAGTTCGGAATTGAAGCAATGGAATTCAACAGAGATCTTATATCTCAGTCTGGTTGGAGAGTTAGTATCATAGC
TAGAGGATATGACAAATACGATAGGTTATTGGCTTATGTGTACACGAAAGACGACAAAATGATAAACGAGGAGATCATAA
GAAACGGTCTCGCAAGACCTTTAACCTACACAAGCACATCAGAACATTCTCAGGATTTCAAAGAAGCCTATGAGGAAGCC
TTTAAAGAGAGACGGGGAATTTTCTCGAAGTATGATCCCGATTCAGACAATCCAGCACCAACTGTTGATGCTTCAGTTGT
AAAAGACAACCTTGATGATTACCTCGGGAAGATCATCTGGTTGAAATTCACTCCAACAGATACCGGTTACGATTCTAAAT
TCGATGAATACTTACTCTTTTCTGATGATGCTGTTATAGCAATTCGTAAAGATGAAGCATACTTGTTTTTTGGCGGTGAT
CCGAAATTCAAAAATTACGTTGGCGAAGAAATAAAGGTTTACGGCGAAATATGGGAAGAAGACGGAAAACCGGAAATACT
GTTAAGAGCACCCTTTGAAATCAAACCAATAGGAGAGTAA

Upstream 100 bases:

>100_bases
ACCGTTATCGGTATCGTTTCGTTGTTCAAAGGAGAATGGGAGATCATTCCAAGATCCCAGGCTGATATACAATAATACAT
CGAGAAAGGAAAGGTGAAAT

Downstream 100 bases:

>100_bases
GAGAATAACAAAATGATTAACCCTAAATACCTTTGAGGGCTCCCTTTGAGCTCCACTCAATAAATTAATTAGGAGGTGTG
GGTATGAAGAAAGTACTGTT

Product: nuclease (SNase domain protein)

Products: NA

Alternate protein names: TNase; Micrococcal nuclease; Staphylococcal nuclease [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSSHRSVIKQSWLYVSLVILVSISALFLTSCPGWFTSSYYVTEIIDGDTIKVLYNGEEVKVRYIGIDTPETHSDYQDSKP
NGEFGIEAMEFNRDLISQSGWRVSIIARGYDKYDRLLAYVYTKDDKMINEEIIRNGLARPLTYTSTSEHSQDFKEAYEEA
FKERRGIFSKYDPDSDNPAPTVDASVVKDNLDDYLGKIIWLKFTPTDTGYDSKFDEYLLFSDDAVIAIRKDEAYLFFGGD
PKFKNYVGEEIKVYGEIWEEDGKPEILLRAPFEIKPIGE

Sequences:

>Translated_279_residues
MSSHRSVIKQSWLYVSLVILVSISALFLTSCPGWFTSSYYVTEIIDGDTIKVLYNGEEVKVRYIGIDTPETHSDYQDSKP
NGEFGIEAMEFNRDLISQSGWRVSIIARGYDKYDRLLAYVYTKDDKMINEEIIRNGLARPLTYTSTSEHSQDFKEAYEEA
FKERRGIFSKYDPDSDNPAPTVDASVVKDNLDDYLGKIIWLKFTPTDTGYDSKFDEYLLFSDDAVIAIRKDEAYLFFGGD
PKFKNYVGEEIKVYGEIWEEDGKPEILLRAPFEIKPIGE
>Mature_278_residues
SSHRSVIKQSWLYVSLVILVSISALFLTSCPGWFTSSYYVTEIIDGDTIKVLYNGEEVKVRYIGIDTPETHSDYQDSKPN
GEFGIEAMEFNRDLISQSGWRVSIIARGYDKYDRLLAYVYTKDDKMINEEIIRNGLARPLTYTSTSEHSQDFKEAYEEAF
KERRGIFSKYDPDSDNPAPTVDASVVKDNLDDYLGKIIWLKFTPTDTGYDSKFDEYLLFSDDAVIAIRKDEAYLFFGGDP
KFKNYVGEEIKVYGEIWEEDGKPEILLRAPFEIKPIGE

Specific function: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Nuclease B:Membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thermonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF00565 SNase [H]

EC number: =3.1.31.1 [H]

Molecular weight: Translated: 32072; Mature: 31941

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50830 TNASE_3 ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHRSVIKQSWLYVSLVILVSISALFLTSCPGWFTSSYYVTEIIDGDTIKVLYNGEEVK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEECCCEEE
VRYIGIDTPETHSDYQDSKPNGEFGIEAMEFNRDLISQSGWRVSIIARGYDKYDRLLAYV
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCEEEEEECCCCHHHHEEEEE
YTKDDKMINEEIIRNGLARPLTYTSTSEHSQDFKEAYEEAFKERRGIFSKYDPDSDNPAP
EECCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TVDASVVKDNLDDYLGKIIWLKFTPTDTGYDSKFDEYLLFSDDAVIAIRKDEAYLFFGGD
CCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEECCEEEEEEECCEEEEECCC
PKFKNYVGEEIKVYGEIWEEDGKPEILLRAPFEIKPIGE
CCHHHHCCCCEEEEEHHHCCCCCCEEEEECCCEEEECCC
>Mature Secondary Structure 
SSHRSVIKQSWLYVSLVILVSISALFLTSCPGWFTSSYYVTEIIDGDTIKVLYNGEEVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEECCCEEE
VRYIGIDTPETHSDYQDSKPNGEFGIEAMEFNRDLISQSGWRVSIIARGYDKYDRLLAYV
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCEEEEEECCCCHHHHEEEEE
YTKDDKMINEEIIRNGLARPLTYTSTSEHSQDFKEAYEEAFKERRGIFSKYDPDSDNPAP
EECCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TVDASVVKDNLDDYLGKIIWLKFTPTDTGYDSKFDEYLLFSDDAVIAIRKDEAYLFFGGD
CCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEECCEEEEEEECCEEEEECCC
PKFKNYVGEEIKVYGEIWEEDGKPEILLRAPFEIKPIGE
CCHHHHCCCCEEEEEHHHCCCCCCEEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6307819; 3921523; 3112123; 893427; 4324890; 4339720; 288045; 1896431; 1731948; 8069625 [H]