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The map label for this gene is metG [H]

Identifier: 239616428

GI number: 239616428

Start: 24079

End: 25998

Strand: Direct

Name: metG [H]

Synonym: Kole_0017

Alternate gene names: 239616428

Gene position: 24079-25998 (Clockwise)

Preceding gene: 239616427

Following gene: 239616429

Centisome position: 1.05

GC content: 44.01

Gene sequence:

>1920_bases
ATGAAGCCGAAGTTTTATGTTACCACACCGATTTATTACATAAATTCTGAACCCCATATAGGTTCTGCATATACCACAAT
CGTTGCCGATATTGTGGCGCGATACAAGCGGCTGAGCGGATACGATGTGTTTTTTCTAACCGGAACCGATGAGCATGGTC
AAAAGGTTCTACAAGCTGCGAAAGAAGAAAACCTTGATCCGCAAACCTACAGCGATCAACTCGCTGAAAAATTTAAGGTT
CTCTGGAAAGAGCTGCAGATTACCAACGATTATTTCGTCAGGACCACAGATGAACGCCACATGAAAACCGTCCAGCTCTT
TGTGAAAAAGATGCTTGAGAATGGCGATGTTTACAAAGGGAAGTACGAAGGATGGTACTGTGTCCCCTGTGAAACATTCT
GGACGGAAGAGGACATCGGAAAAGAAAGAGTTTGCCCGTCCTGCGGCAGAGAGGTCAAATGGGTGAGCGAGGAAAATTAC
TTCTTCAGGCTTTCAAAATATAACGACGCTTTGCTCAAACACTTTCAGGAGCACCCGGAATTTGTACAACCTGATTTTCG
AAGAAACGAAATGTTGAGAATACTCGAAAATGGCCTCAGGGATCTGAGCATTACCCGCACCTCTTTCAAGTGGGGAGTTC
CAATGCCGAACGACCCTGAACATGTTATATACGTTTGGGTTGATGCTTTGATAAATTACATAAGCGCTATCGGATATATA
GATGACCCCGAGACATTCCATAGATATTGGCCGGCTGATGTACATATCATTGGAAAAGAAATAAATCGCTTTCATTCGCT
AATATGGCCTGCAATGCTGATGTCTGTCGGGCTTCCTTTGCCAAAACAGATCTATGCGCACGGCTGGTTGACGGTAAATG
GCCAGAAGATATCCAAATCCCTTGGTAACGCCATAGACCCAAGGGAATTCGTAAAAGCCTATGGAAACGATGCCGTTCGA
TACTATCTTTTAAGGGACATACAGTTTGGCAGAGACGGAGATTTTTCGGAGGAAAATCTGGTACACAGAATAAATGCTGA
CCTGGCCAATGATCTTGGGAACCTTCTGCACAGAACGGTTGCCATGATCGAGAAATTCAACAACGGCATTGTTCCTAAAA
AGGGCGTTGTTGAAGACGTAGACAACCAGCTTTACGGGCTGGCAGAGGAGGTTATAGCGGAATACATAGATCACATGGAT
AATCTCAGATTCACCCCTTCACTGGATGCAATCTGGAGGTTCGCCAGATTTGGAAATAAGTATATCGATCTTACAGAACC
ATGGAAACTGGGGAAAGACCCAGAGAAGGCTGAAAGACTGAACACGGTTCTTTACAATCTTGTAGATACACTTCGAATAG
TTTCGCTGATGATTGAGCCTGTTATGCCAAATACCGCAAAAGCAATCAGAGAGAGGATAGGACAGGCAGTGGAACTAGAC
TTTACGCACTTGAAATTGGGATTAACAAAGAACGGAGTAGGCGTTAAGCACGGTGAACCTTTGTTCCCGAGAATAGATGT
TAAAAAGCACAGGTGGGTGAGTAAAATGCTGGAAGAGAAAAAAGCAGAAGCGTCTGTGGAAGGAAAAGAAGATAACCTTA
TAGAGATAAGTGATTTTGCTAAGGTGGAACTTCGTGTCGGCGAAATCCTGGAAGCTGAAAAGGTGGAGAAGTCACGAAAA
TTAATAAAACTCAAAATAAATCTTGGCGAGCTTGGCGAGCGCCAGATTGTTGCCGGAATCGCCCGGCATTATAATCCTGA
AGAGCTAATTGGGCTCAAGATTGTGGTTGTAACCAACTTGAAACCTGCAAAGCTTATGGGCATCGAATCTCAGGGCATGC
TCTTGGCAGCAAAAATCGAAGACTCTTTAACAGTATTGACCGTTCACAGGGATATAAAACCAGGCGCAAAAGTTTCTTGA

Upstream 100 bases:

>100_bases
ACGAAAAAATCAGCACTAATTAGGTAAATTCTGATAAAATCAGGAATACAGGCGTATAAACCATGATATAATTAAAATAA
CCAAAAGACGGAGGTGTACT

Downstream 100 bases:

>100_bases
GAGAGGAGGCACTACTTTGCCCGAGGAAATAATATCGAGATCAATAAACGATGAAATGATAACTTCTTATATGCTCTATT
CCATGAGCGTTATTGTCGGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MKPKFYVTTPIYYINSEPHIGSAYTTIVADIVARYKRLSGYDVFFLTGTDEHGQKVLQAAKEENLDPQTYSDQLAEKFKV
LWKELQITNDYFVRTTDERHMKTVQLFVKKMLENGDVYKGKYEGWYCVPCETFWTEEDIGKERVCPSCGREVKWVSEENY
FFRLSKYNDALLKHFQEHPEFVQPDFRRNEMLRILENGLRDLSITRTSFKWGVPMPNDPEHVIYVWVDALINYISAIGYI
DDPETFHRYWPADVHIIGKEINRFHSLIWPAMLMSVGLPLPKQIYAHGWLTVNGQKISKSLGNAIDPREFVKAYGNDAVR
YYLLRDIQFGRDGDFSEENLVHRINADLANDLGNLLHRTVAMIEKFNNGIVPKKGVVEDVDNQLYGLAEEVIAEYIDHMD
NLRFTPSLDAIWRFARFGNKYIDLTEPWKLGKDPEKAERLNTVLYNLVDTLRIVSLMIEPVMPNTAKAIRERIGQAVELD
FTHLKLGLTKNGVGVKHGEPLFPRIDVKKHRWVSKMLEEKKAEASVEGKEDNLIEISDFAKVELRVGEILEAEKVEKSRK
LIKLKINLGELGERQIVAGIARHYNPEELIGLKIVVVTNLKPAKLMGIESQGMLLAAKIEDSLTVLTVHRDIKPGAKVS

Sequences:

>Translated_639_residues
MKPKFYVTTPIYYINSEPHIGSAYTTIVADIVARYKRLSGYDVFFLTGTDEHGQKVLQAAKEENLDPQTYSDQLAEKFKV
LWKELQITNDYFVRTTDERHMKTVQLFVKKMLENGDVYKGKYEGWYCVPCETFWTEEDIGKERVCPSCGREVKWVSEENY
FFRLSKYNDALLKHFQEHPEFVQPDFRRNEMLRILENGLRDLSITRTSFKWGVPMPNDPEHVIYVWVDALINYISAIGYI
DDPETFHRYWPADVHIIGKEINRFHSLIWPAMLMSVGLPLPKQIYAHGWLTVNGQKISKSLGNAIDPREFVKAYGNDAVR
YYLLRDIQFGRDGDFSEENLVHRINADLANDLGNLLHRTVAMIEKFNNGIVPKKGVVEDVDNQLYGLAEEVIAEYIDHMD
NLRFTPSLDAIWRFARFGNKYIDLTEPWKLGKDPEKAERLNTVLYNLVDTLRIVSLMIEPVMPNTAKAIRERIGQAVELD
FTHLKLGLTKNGVGVKHGEPLFPRIDVKKHRWVSKMLEEKKAEASVEGKEDNLIEISDFAKVELRVGEILEAEKVEKSRK
LIKLKINLGELGERQIVAGIARHYNPEELIGLKIVVVTNLKPAKLMGIESQGMLLAAKIEDSLTVLTVHRDIKPGAKVS
>Mature_639_residues
MKPKFYVTTPIYYINSEPHIGSAYTTIVADIVARYKRLSGYDVFFLTGTDEHGQKVLQAAKEENLDPQTYSDQLAEKFKV
LWKELQITNDYFVRTTDERHMKTVQLFVKKMLENGDVYKGKYEGWYCVPCETFWTEEDIGKERVCPSCGREVKWVSEENY
FFRLSKYNDALLKHFQEHPEFVQPDFRRNEMLRILENGLRDLSITRTSFKWGVPMPNDPEHVIYVWVDALINYISAIGYI
DDPETFHRYWPADVHIIGKEINRFHSLIWPAMLMSVGLPLPKQIYAHGWLTVNGQKISKSLGNAIDPREFVKAYGNDAVR
YYLLRDIQFGRDGDFSEENLVHRINADLANDLGNLLHRTVAMIEKFNNGIVPKKGVVEDVDNQLYGLAEEVIAEYIDHMD
NLRFTPSLDAIWRFARFGNKYIDLTEPWKLGKDPEKAERLNTVLYNLVDTLRIVSLMIEPVMPNTAKAIRERIGQAVELD
FTHLKLGLTKNGVGVKHGEPLFPRIDVKKHRWVSKMLEEKKAEASVEGKEDNLIEISDFAKVELRVGEILEAEKVEKSRK
LIKLKINLGELGERQIVAGIARHYNPEELIGLKIVVVTNLKPAKLMGIESQGMLLAAKIEDSLTVLTVHRDIKPGAKVS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=551, Percent_Identity=36.6606170598911, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI14043022, Length=515, Percent_Identity=28.9320388349515, Blast_Score=179, Evalue=9e-45,
Organism=Homo sapiens, GI4507947, Length=95, Percent_Identity=38.9473684210526, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1788432, Length=691, Percent_Identity=29.0882778581766, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=35.5140186915888, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=492, Percent_Identity=34.5528455284553, Blast_Score=314, Evalue=9e-86,
Organism=Caenorhabditis elegans, GI71992781, Length=382, Percent_Identity=32.4607329842932, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17540794, Length=544, Percent_Identity=25.9191176470588, Blast_Score=175, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321610, Length=528, Percent_Identity=33.3333333333333, Blast_Score=299, Evalue=7e-82,
Organism=Saccharomyces cerevisiae, GI6321704, Length=570, Percent_Identity=25.6140350877193, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21358483, Length=493, Percent_Identity=34.0770791075051, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24655311, Length=519, Percent_Identity=28.3236994219653, Blast_Score=181, Evalue=2e-45,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 73561; Mature: 73561

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKFYVTTPIYYINSEPHIGSAYTTIVADIVARYKRLSGYDVFFLTGTDEHGQKVLQAA
CCCCEEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
KEENLDPQTYSDQLAEKFKVLWKELQITNDYFVRTTDERHMKTVQLFVKKMLENGDVYKG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEC
KYEGWYCVPCETFWTEEDIGKERVCPSCGREVKWVSEENYFFRLSKYNDALLKHFQEHPE
CCCCEEEECCHHHCCHHHCCHHHCCHHCCCCEEEEECCCEEEEEECCCHHHHHHHHHCCC
FVQPDFRRNEMLRILENGLRDLSITRTSFKWGVPMPNDPEHVIYVWVDALINYISAIGYI
CCCCCCHHHHHHHHHHCCCHHEEEEEECEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCC
DDPETFHRYWPADVHIIGKEINRFHSLIWPAMLMSVGLPLPKQIYAHGWLTVNGQKISKS
CCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEECHHHHHHH
LGNAIDPREFVKAYGNDAVRYYLLRDIQFGRDGDFSEENLVHRINADLANDLGNLLHRTV
HCCCCCHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AMIEKFNNGIVPKKGVVEDVDNQLYGLAEEVIAEYIDHMDNLRFTPSLDAIWRFARFGNK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
YIDLTEPWKLGKDPEKAERLNTVLYNLVDTLRIVSLMIEPVMPNTAKAIRERIGQAVELD
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEE
FTHLKLGLTKNGVGVKHGEPLFPRIDVKKHRWVSKMLEEKKAEASVEGKEDNLIEISDFA
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
KVELRVGEILEAEKVEKSRKLIKLKINLGELGERQIVAGIARHYNPEELIGLKIVVVTNL
EEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHEEEEEEEECC
KPAKLMGIESQGMLLAAKIEDSLTVLTVHRDIKPGAKVS
CCHHEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKPKFYVTTPIYYINSEPHIGSAYTTIVADIVARYKRLSGYDVFFLTGTDEHGQKVLQAA
CCCCEEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
KEENLDPQTYSDQLAEKFKVLWKELQITNDYFVRTTDERHMKTVQLFVKKMLENGDVYKG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEC
KYEGWYCVPCETFWTEEDIGKERVCPSCGREVKWVSEENYFFRLSKYNDALLKHFQEHPE
CCCCEEEECCHHHCCHHHCCHHHCCHHCCCCEEEEECCCEEEEEECCCHHHHHHHHHCCC
FVQPDFRRNEMLRILENGLRDLSITRTSFKWGVPMPNDPEHVIYVWVDALINYISAIGYI
CCCCCCHHHHHHHHHHCCCHHEEEEEECEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCC
DDPETFHRYWPADVHIIGKEINRFHSLIWPAMLMSVGLPLPKQIYAHGWLTVNGQKISKS
CCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEECHHHHHHH
LGNAIDPREFVKAYGNDAVRYYLLRDIQFGRDGDFSEENLVHRINADLANDLGNLLHRTV
HCCCCCHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AMIEKFNNGIVPKKGVVEDVDNQLYGLAEEVIAEYIDHMDNLRFTPSLDAIWRFARFGNK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
YIDLTEPWKLGKDPEKAERLNTVLYNLVDTLRIVSLMIEPVMPNTAKAIRERIGQAVELD
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEE
FTHLKLGLTKNGVGVKHGEPLFPRIDVKKHRWVSKMLEEKKAEASVEGKEDNLIEISDFA
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
KVELRVGEILEAEKVEKSRKLIKLKINLGELGERQIVAGIARHYNPEELIGLKIVVVTNL
EEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHEEEEEEEECC
KPAKLMGIESQGMLLAAKIEDSLTVLTVHRDIKPGAKVS
CCHHEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9380682; 10360571 [H]