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The map label for this gene is gyrA [H]

Identifier: 239616429

GI number: 239616429

Start: 26015

End: 28453

Strand: Direct

Name: gyrA [H]

Synonym: Kole_0018

Alternate gene names: 239616429

Gene position: 26015-28453 (Clockwise)

Preceding gene: 239616428

Following gene: 239616430

Centisome position: 1.13

GC content: 46.21

Gene sequence:

>2439_bases
TTGCCCGAGGAAATAATATCGAGATCAATAAACGATGAAATGATAACTTCTTATATGCTCTATTCCATGAGCGTTATTGT
CGGGCGTGCTATTCCCGATGTAAGAGACGGTTTGAAACCGGTTCAAAGAAAGATCCTTTTTGGTATGCTGGAGCTCGGCC
TCCGTCATAACCAATCTTACAAAAAGAGCGCAAGAATCGTTGGTGAGGTAATGGGTAAATTCCATCCTCATGGTGACATG
GCGATCTACGATGCCCTTGTGAGGATGGCTCAACCCTTTTCTATGCGTTATCCCTTAATTCAAGGGCAGGGAAATTTTGG
TTCTATAGACCGTGACCCACCCGCGGCAATGCGTTATACCGAAGCCCGTATGCAACGATTGGCCGAAGAATTGCTCGCAG
ACATTGATAAGAATACAGTGAAGATGATTCCAAACTTCGATGGTTCTCTTATAGAGCCAGAGGTTCTTCCGGCTAAAGCT
CCCAACCTGCTGATGAACGGTGCTTCAGGTATCGCTGTTGGAATGATGACCAACATTCCACCCCACAATCTCTCCGAACT
TGTTGAAGCTCTAACAGCGCTTATAGACAACCCAGATGCCACCATCGAAGAGTTGATGGAGTATGTGAAAGGCCCTGACT
TTCCTACCGGCGGTATTATTATGGGCCGTGACGGCATCAAAAAGATGTATGAAACCGGTCGCGGCAGAATGGTAGTTCGT
GGGGTCGCGGAGATTGAAGAAGCAAAGGGTGGCACCAGGATCGTAATAAGCGAGATACCTTACGGCGTATCAAAGGCCGA
CTTGATCCAGCAGATAGCAAATGTAGCTCAGAATGTACGGGATATCCAGGTAAGAAATGTCAGAGATGAATCAGACAAAC
GAGGGCTTCGAGTGGTTATCGAGTTGAAACGAGGCGCCGATCCTAACGTTGTTTTGAACCTTCTTTATAAACACACACAG
CTTCAAACAACCTTTGGCGCACACATGCTGGTGATAGATGAGAAAAAGCGCCCGAAATTGATGAACCTCAAGGAGATATT
CCAGGCCTTCATCAAACATCGATATGAGGTTGTAAAGAGGCGCACAGAGTATGAACTTGAACAAGCTTCTAAGAAGGCCC
ATATTCTCGAAGGTCTGACGAAAGCTTCAAGAGCCATCGATACAGTCGTTGATATCATCAGAAATTCAAAGAACATCCAG
GAAGCTTCCGTAAATTTGCAGGAGACACTTGAGATAACTCCGGAGCAGAGCCAGGCAATACTCGAGATGAGACTCGGAAA
ACTAACCGCTCTGGAGATCGATAAACTCGTTACTGAATATGCTGAGCTGGTAGAAAAAATAAAAGAGTACAGAGAGATTC
TCTCCGACGATAAAAACATATACCAGATCATCAAAAAAGAGCTTCAGGAACTTGAGGCCCAGTACGGCGATGCTCGCAGA
ACAAAAATAAGTATAGATGGGAACACCGATTTTAACGTCGAAGATGTTATCCCGGATGATGAAGTGGTCGTCACCGTCAC
CAAAAAAGGTTATATAAAAGCGACACCGCTTGAGGACTACCGCAAGCAGGGTAGAGGCGGAAAAGGTATAAGGGGTGTCA
AAACCACCGATGCTGACTTCGTCACAAACGTGGTAAGTACGACCCGTCTCAGTAAAACCGTTGTTATCACATCCAAGGGT
AAAGCTTACTTTATAAACAACTATGAGCTCGAATGTACTTCACGCAGTTCCAGAGGCAAACTCCTGGCCAATTACGTGAA
AATCGAACCCGACGAAACCGTGCAAGCGGTTCTATCCGTAAAACGCGAAGAAGTCGCAAACAAGCATCTAATAATTACGA
CCAGAAAGGGCAAAATAAAGCGTACCCCCTTTGAAGCCTTTATCAACTCGCGAACATCCGGTATAAAGGCTATAACACTC
AACGAAGGTGATAGTGTCGTTGATGCTGGCATCAGCACTTCGGAAGAAGAAACCATAATTATCTCCACACGCAAAGGAAT
GGTTATAAGGTTCCCGATTTCGCAGATCAGACCCATGGGACGTACAGCAGCCGGTGTCAAAGCGATGGCCCTTCGTGGTG
ATGATGAGGTCGTCTCCGCTACGATAGTACTCCCGGTTGATGAACGCTATCTTTTCACAGCTACAGAAAGAGGCGTAGGA
AAACGAACGCCTCTCAGTGAATACAGACCCCAGCACCGTGCTGGCATGGGTGTGAAGAATATCTACGGTCTCGAAAGAAC
AGGATATGTTGTGGGTTCTCTTGTGGTAACCAATGAAGACGAAATCATAGTTATAACCAAAAACGGTATGTCCATAAGAA
TCCCGGCTGCTGATATAAGGCCGACGGGAAGAGTAACAAAGGGAGTTAAGGTTGTGGAGCTTCGTGATGACGATACGGTG
GCGAGTCTCGCCGTTGTTGTTGACCAGGCGGAAGTATAG

Upstream 100 bases:

>100_bases
ATGCTCTTGGCAGCAAAAATCGAAGACTCTTTAACAGTATTGACCGTTCACAGGGATATAAAACCAGGCGCAAAAGTTTC
TTGAGAGAGGAGGCACTACT

Downstream 100 bases:

>100_bases
TTCAGGAGGTGGGTTATCCGTGAACCTGACAGAACGCCAGAGGGAAATTCTGGAATACATAGAAAAGTTCATAAAACTCA
ATGGTTATCCGCCAAGCATC

Product: DNA gyrase, A subunit

Products: NA

Alternate protein names: Topoisomerase II subunit A [H]

Number of amino acids: Translated: 812; Mature: 811

Protein sequence:

>812_residues
MPEEIISRSINDEMITSYMLYSMSVIVGRAIPDVRDGLKPVQRKILFGMLELGLRHNQSYKKSARIVGEVMGKFHPHGDM
AIYDALVRMAQPFSMRYPLIQGQGNFGSIDRDPPAAMRYTEARMQRLAEELLADIDKNTVKMIPNFDGSLIEPEVLPAKA
PNLLMNGASGIAVGMMTNIPPHNLSELVEALTALIDNPDATIEELMEYVKGPDFPTGGIIMGRDGIKKMYETGRGRMVVR
GVAEIEEAKGGTRIVISEIPYGVSKADLIQQIANVAQNVRDIQVRNVRDESDKRGLRVVIELKRGADPNVVLNLLYKHTQ
LQTTFGAHMLVIDEKKRPKLMNLKEIFQAFIKHRYEVVKRRTEYELEQASKKAHILEGLTKASRAIDTVVDIIRNSKNIQ
EASVNLQETLEITPEQSQAILEMRLGKLTALEIDKLVTEYAELVEKIKEYREILSDDKNIYQIIKKELQELEAQYGDARR
TKISIDGNTDFNVEDVIPDDEVVVTVTKKGYIKATPLEDYRKQGRGGKGIRGVKTTDADFVTNVVSTTRLSKTVVITSKG
KAYFINNYELECTSRSSRGKLLANYVKIEPDETVQAVLSVKREEVANKHLIITTRKGKIKRTPFEAFINSRTSGIKAITL
NEGDSVVDAGISTSEEETIIISTRKGMVIRFPISQIRPMGRTAAGVKAMALRGDDEVVSATIVLPVDERYLFTATERGVG
KRTPLSEYRPQHRAGMGVKNIYGLERTGYVVGSLVVTNEDEIIVITKNGMSIRIPAADIRPTGRVTKGVKVVELRDDDTV
ASLAVVVDQAEV

Sequences:

>Translated_812_residues
MPEEIISRSINDEMITSYMLYSMSVIVGRAIPDVRDGLKPVQRKILFGMLELGLRHNQSYKKSARIVGEVMGKFHPHGDM
AIYDALVRMAQPFSMRYPLIQGQGNFGSIDRDPPAAMRYTEARMQRLAEELLADIDKNTVKMIPNFDGSLIEPEVLPAKA
PNLLMNGASGIAVGMMTNIPPHNLSELVEALTALIDNPDATIEELMEYVKGPDFPTGGIIMGRDGIKKMYETGRGRMVVR
GVAEIEEAKGGTRIVISEIPYGVSKADLIQQIANVAQNVRDIQVRNVRDESDKRGLRVVIELKRGADPNVVLNLLYKHTQ
LQTTFGAHMLVIDEKKRPKLMNLKEIFQAFIKHRYEVVKRRTEYELEQASKKAHILEGLTKASRAIDTVVDIIRNSKNIQ
EASVNLQETLEITPEQSQAILEMRLGKLTALEIDKLVTEYAELVEKIKEYREILSDDKNIYQIIKKELQELEAQYGDARR
TKISIDGNTDFNVEDVIPDDEVVVTVTKKGYIKATPLEDYRKQGRGGKGIRGVKTTDADFVTNVVSTTRLSKTVVITSKG
KAYFINNYELECTSRSSRGKLLANYVKIEPDETVQAVLSVKREEVANKHLIITTRKGKIKRTPFEAFINSRTSGIKAITL
NEGDSVVDAGISTSEEETIIISTRKGMVIRFPISQIRPMGRTAAGVKAMALRGDDEVVSATIVLPVDERYLFTATERGVG
KRTPLSEYRPQHRAGMGVKNIYGLERTGYVVGSLVVTNEDEIIVITKNGMSIRIPAADIRPTGRVTKGVKVVELRDDDTV
ASLAVVVDQAEV
>Mature_811_residues
PEEIISRSINDEMITSYMLYSMSVIVGRAIPDVRDGLKPVQRKILFGMLELGLRHNQSYKKSARIVGEVMGKFHPHGDMA
IYDALVRMAQPFSMRYPLIQGQGNFGSIDRDPPAAMRYTEARMQRLAEELLADIDKNTVKMIPNFDGSLIEPEVLPAKAP
NLLMNGASGIAVGMMTNIPPHNLSELVEALTALIDNPDATIEELMEYVKGPDFPTGGIIMGRDGIKKMYETGRGRMVVRG
VAEIEEAKGGTRIVISEIPYGVSKADLIQQIANVAQNVRDIQVRNVRDESDKRGLRVVIELKRGADPNVVLNLLYKHTQL
QTTFGAHMLVIDEKKRPKLMNLKEIFQAFIKHRYEVVKRRTEYELEQASKKAHILEGLTKASRAIDTVVDIIRNSKNIQE
ASVNLQETLEITPEQSQAILEMRLGKLTALEIDKLVTEYAELVEKIKEYREILSDDKNIYQIIKKELQELEAQYGDARRT
KISIDGNTDFNVEDVIPDDEVVVTVTKKGYIKATPLEDYRKQGRGGKGIRGVKTTDADFVTNVVSTTRLSKTVVITSKGK
AYFINNYELECTSRSSRGKLLANYVKIEPDETVQAVLSVKREEVANKHLIITTRKGKIKRTPFEAFINSRTSGIKAITLN
EGDSVVDAGISTSEEETIIISTRKGMVIRFPISQIRPMGRTAAGVKAMALRGDDEVVSATIVLPVDERYLFTATERGVGK
RTPLSEYRPQHRAGMGVKNIYGLERTGYVVGSLVVTNEDEIIVITKNGMSIRIPAADIRPTGRVTKGVKVVELRDDDTVA
SLAVVVDQAEV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=215, Percent_Identity=33.953488372093, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI19913408, Length=197, Percent_Identity=33.502538071066, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1788562, Length=844, Percent_Identity=43.7203791469194, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=702, Percent_Identity=36.4672364672365, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI212645657, Length=192, Percent_Identity=31.7708333333333, Blast_Score=82, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535065, Length=173, Percent_Identity=32.3699421965318, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115534553, Length=169, Percent_Identity=31.3609467455621, Blast_Score=77, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI212645845, Length=449, Percent_Identity=22.9398663697105, Blast_Score=76, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=271, Percent_Identity=30.6273062730627, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17136538, Length=366, Percent_Identity=26.5027322404372, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90486; Mature: 90355

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEEIISRSINDEMITSYMLYSMSVIVGRAIPDVRDGLKPVQRKILFGMLELGLRHNQSY
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KKSARIVGEVMGKFHPHGDMAIYDALVRMAQPFSMRYPLIQGQGNFGSIDRDPPAAMRYT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
EARMQRLAEELLADIDKNTVKMIPNFDGSLIEPEVLPAKAPNLLMNGASGIAVGMMTNIP
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCC
PHNLSELVEALTALIDNPDATIEELMEYVKGPDFPTGGIIMGRDGIKKMYETGRGRMVVR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHCCCCEEEEE
GVAEIEEAKGGTRIVISEIPYGVSKADLIQQIANVAQNVRDIQVRNVRDESDKRGLRVVI
EHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEE
ELKRGADPNVVLNLLYKHTQLQTTFGAHMLVIDEKKRPKLMNLKEIFQAFIKHRYEVVKR
EECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RTEYELEQASKKAHILEGLTKASRAIDTVVDIIRNSKNIQEASVNLQETLEITPEQSQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHEECCCCHHHHH
LEMRLGKLTALEIDKLVTEYAELVEKIKEYREILSDDKNIYQIIKKELQELEAQYGDARR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCE
TKISIDGNTDFNVEDVIPDDEVVVTVTKKGYIKATPLEDYRKQGRGGKGIRGVKTTDADF
EEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCHHHHHHCCCCCCCCCCCCCCCHHH
VTNVVSTTRLSKTVVITSKGKAYFINNYELECTSRSSRGKLLANYVKIEPDETVQAVLSV
HHHHHHHHHCCEEEEEEECCCEEEEECEEEEECCCCCCCCEEEEEEEECCHHHHHHHHHH
KREEVANKHLIITTRKGKIKRTPFEAFINSRTSGIKAITLNEGDSVVDAGISTSEEETII
HHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEECCCCCCCCCEEE
ISTRKGMVIRFPISQIRPMGRTAAGVKAMALRGDDEVVSATIVLPVDERYLFTATERGVG
EEECCCEEEEECHHHCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEEECCCCCC
KRTPLSEYRPQHRAGMGVKNIYGLERTGYVVGSLVVTNEDEIIVITKNGMSIRIPAADIR
CCCCHHHCCCHHHHCCCHHHHCCCCCCCEEEEEEEEECCCCEEEEEECCCEEEECCCCCC
PTGRVTKGVKVVELRDDDTVASLAVVVDQAEV
CCCCCCCCEEEEEECCCCHHHHHHHEEECCCC
>Mature Secondary Structure 
PEEIISRSINDEMITSYMLYSMSVIVGRAIPDVRDGLKPVQRKILFGMLELGLRHNQSY
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KKSARIVGEVMGKFHPHGDMAIYDALVRMAQPFSMRYPLIQGQGNFGSIDRDPPAAMRYT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
EARMQRLAEELLADIDKNTVKMIPNFDGSLIEPEVLPAKAPNLLMNGASGIAVGMMTNIP
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCC
PHNLSELVEALTALIDNPDATIEELMEYVKGPDFPTGGIIMGRDGIKKMYETGRGRMVVR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHCCCCEEEEE
GVAEIEEAKGGTRIVISEIPYGVSKADLIQQIANVAQNVRDIQVRNVRDESDKRGLRVVI
EHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEE
ELKRGADPNVVLNLLYKHTQLQTTFGAHMLVIDEKKRPKLMNLKEIFQAFIKHRYEVVKR
EECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RTEYELEQASKKAHILEGLTKASRAIDTVVDIIRNSKNIQEASVNLQETLEITPEQSQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHEECCCCHHHHH
LEMRLGKLTALEIDKLVTEYAELVEKIKEYREILSDDKNIYQIIKKELQELEAQYGDARR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCE
TKISIDGNTDFNVEDVIPDDEVVVTVTKKGYIKATPLEDYRKQGRGGKGIRGVKTTDADF
EEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCHHHHHHCCCCCCCCCCCCCCCHHH
VTNVVSTTRLSKTVVITSKGKAYFINNYELECTSRSSRGKLLANYVKIEPDETVQAVLSV
HHHHHHHHHCCEEEEEEECCCEEEEECEEEEECCCCCCCCEEEEEEEECCHHHHHHHHHH
KREEVANKHLIITTRKGKIKRTPFEAFINSRTSGIKAITLNEGDSVVDAGISTSEEETII
HHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEECCCCCCCCCEEE
ISTRKGMVIRFPISQIRPMGRTAAGVKAMALRGDDEVVSATIVLPVDERYLFTATERGVG
EEECCCEEEEECHHHCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEEECCCCCC
KRTPLSEYRPQHRAGMGVKNIYGLERTGYVVGSLVVTNEDEIIVITKNGMSIRIPAADIR
CCCCHHHCCCHHHHCCCHHHHCCCCCCCEEEEEEEEECCCCEEEEEECCCEEEECCCCCC
PTGRVTKGVKVVELRDDDTVASLAVVVDQAEV
CCCCCCCCEEEEEECCCCHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9380682; 10360571 [H]