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The map label for this gene is murF [H]

Identifier: 238925282

GI number: 238925282

Start: 2819363

End: 2820757

Strand: Reverse

Name: murF [H]

Synonym: EUBREC_2936

Alternate gene names: 238925282

Gene position: 2820757-2819363 (Counterclockwise)

Preceding gene: 238925283

Following gene: 238925281

Centisome position: 81.77

GC content: 48.96

Gene sequence:

>1395_bases
ATGTCGGATAAGTACATGAAGAATCTTACATTTGCAAATATTGCAAAGGCCTGTGAGGGCACATACATAGGCGATGAGAC
ACTGCTTGATACGACAATCACCGGCGCCGTCATAGACAGCAGACAGGTGAAGGAGGGCTATCTTTTTATCCCGATAAAGG
GAGAAAGGGTGGACGGACACAAATTCATTCCGACTGTGTTTGAGCAGGGGGCGGCAATTGTGCTCTCTGAGCATGAGCTG
ACGGATCCGGCAGGCCCATATGTGCTTGTTGAATCAACCACCGATGCCATGAAAAAGCTCGCGGCATTTTACAGAAAAAG
CCTAGATATCAAGGTGGTGGGCATCACCGGAAGTGTCGGCAAGACCAGCACCAAGGAGATGATATCATCAGTTCTTGAGC
AGAAATACAGCGTCCACAAGACCGCAGGCAATTTCAACAACGAGATAGGCCTGCCACTCACTATTTTCGGAATCAGGGAA
AGTCATCAGGTAGCGGTGCTTGAGATGGGCATATCTGATTTTGGCGAGATGCACAGACTTTCTACTATGTCGTGCCCGGA
TGTTATGGTCATCACCAATATCGGCTACTGCCATCTGGAGTTTTTGGGCGACAGGGACGGTGTGCTTAAAGCCAAGACAG
AGTGCTTTGAGCATATGATGCCGGATGCGGTCGCAGTGCTGAATGCAGATGACGACAAGCTCGCCACAGTGCAGACGGTA
AATGGAAAGCCTGCCATATACTATGGCAAGGAATCGGCTTCAGGCGTTCATAAATCAGTATATACCACAAATATTGAAAA
CCTGGGATTTGACGGCATGAAGGCTCATTTTGTCACTCCGGAGGGTGAGTTCGATGCACACATCCACATCCCGGGTGAGC
ACAATGTATACAATGCCATGGCGGCAGCGGCTGTCGGATTGCAGCTTGGCCTCACAATAGCTGAGATAAAATCAGGAATT
GAGAAAGCAGAGACAATCGCAGGCCGTACCAATTTTATCAAAACACACGGCATGACAGTGATAGATGACTGCTACAATGC
AAACCCTGTATCCATGAAATCCTCTATCGAGGTGCTTTCACATGCAAAGGGCAGGACAATAGCAGTGCTTGGCGACATGG
GCGAGCTGGGAAGCGATGAGAAAAAGTTGCACCATGAGGTAGGCGAAGCAGTTGGAGAGAATCATATCCACACTCTTTTT
GCCGCAGGAGAGCTTGCAAAGGAATATGCAGCCGGGGCAGCGGAGAAATCATCCGATGTAGACATCCACTATTTCGAGGA
TAGGGAGACCATGACACAGGAGCTTCTTTCATATGTGAAGGAGGGCGACACCATACTGGTGAAGGCATCACACTTCATGG
AGTTTCCGAAGGTGGTAGAGGCTCTGAGTAAGTAA

Upstream 100 bases:

>100_bases
ACCAGTCTTCTGCCACAGGAGGCAGCGGTTATCGGTATGAGCTTTAATCAGCTCTGTGAGCATCTGATTGACATATCTTT
GAAAAAATATGAGGTGTAGC

Downstream 100 bases:

>100_bases
AATTAGGTAAAATCAATCGGGTAGAAATTAATTCGATATTTAATTATTGCGAGGAATGGATATTCTCCAAGCCTCATAAT
ATTATAAAAGAAAGAGAGAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl- D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSDKYMKNLTFANIAKACEGTYIGDETLLDTTITGAVIDSRQVKEGYLFIPIKGERVDGHKFIPTVFEQGAAIVLSEHEL
TDPAGPYVLVESTTDAMKKLAAFYRKSLDIKVVGITGSVGKTSTKEMISSVLEQKYSVHKTAGNFNNEIGLPLTIFGIRE
SHQVAVLEMGISDFGEMHRLSTMSCPDVMVITNIGYCHLEFLGDRDGVLKAKTECFEHMMPDAVAVLNADDDKLATVQTV
NGKPAIYYGKESASGVHKSVYTTNIENLGFDGMKAHFVTPEGEFDAHIHIPGEHNVYNAMAAAAVGLQLGLTIAEIKSGI
EKAETIAGRTNFIKTHGMTVIDDCYNANPVSMKSSIEVLSHAKGRTIAVLGDMGELGSDEKKLHHEVGEAVGENHIHTLF
AAGELAKEYAAGAAEKSSDVDIHYFEDRETMTQELLSYVKEGDTILVKASHFMEFPKVVEALSK

Sequences:

>Translated_464_residues
MSDKYMKNLTFANIAKACEGTYIGDETLLDTTITGAVIDSRQVKEGYLFIPIKGERVDGHKFIPTVFEQGAAIVLSEHEL
TDPAGPYVLVESTTDAMKKLAAFYRKSLDIKVVGITGSVGKTSTKEMISSVLEQKYSVHKTAGNFNNEIGLPLTIFGIRE
SHQVAVLEMGISDFGEMHRLSTMSCPDVMVITNIGYCHLEFLGDRDGVLKAKTECFEHMMPDAVAVLNADDDKLATVQTV
NGKPAIYYGKESASGVHKSVYTTNIENLGFDGMKAHFVTPEGEFDAHIHIPGEHNVYNAMAAAAVGLQLGLTIAEIKSGI
EKAETIAGRTNFIKTHGMTVIDDCYNANPVSMKSSIEVLSHAKGRTIAVLGDMGELGSDEKKLHHEVGEAVGENHIHTLF
AAGELAKEYAAGAAEKSSDVDIHYFEDRETMTQELLSYVKEGDTILVKASHFMEFPKVVEALSK
>Mature_463_residues
SDKYMKNLTFANIAKACEGTYIGDETLLDTTITGAVIDSRQVKEGYLFIPIKGERVDGHKFIPTVFEQGAAIVLSEHELT
DPAGPYVLVESTTDAMKKLAAFYRKSLDIKVVGITGSVGKTSTKEMISSVLEQKYSVHKTAGNFNNEIGLPLTIFGIRES
HQVAVLEMGISDFGEMHRLSTMSCPDVMVITNIGYCHLEFLGDRDGVLKAKTECFEHMMPDAVAVLNADDDKLATVQTVN
GKPAIYYGKESASGVHKSVYTTNIENLGFDGMKAHFVTPEGEFDAHIHIPGEHNVYNAMAAAAVGLQLGLTIAEIKSGIE
KAETIAGRTNFIKTHGMTVIDDCYNANPVSMKSSIEVLSHAKGRTIAVLGDMGELGSDEKKLHHEVGEAVGENHIHTLFA
AGELAKEYAAGAAEKSSDVDIHYFEDRETMTQELLSYVKEGDTILVKASHFMEFPKVVEALSK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=464, Percent_Identity=32.7586206896552, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50478; Mature: 50347

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKYMKNLTFANIAKACEGTYIGDETLLDTTITGAVIDSRQVKEGYLFIPIKGERVDGH
CCCHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHEEECCCCCCCCEEEEEEECCCCCCC
KFIPTVFEQGAAIVLSEHELTDPAGPYVLVESTTDAMKKLAAFYRKSLDIKVVGITGSVG
CCCHHHHCCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCC
KTSTKEMISSVLEQKYSVHKTAGNFNNEIGLPLTIFGIRESHQVAVLEMGISDFGEMHRL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHHH
STMSCPDVMVITNIGYCHLEFLGDRDGVLKAKTECFEHMMPDAVAVLNADDDKLATVQTV
CCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEE
NGKPAIYYGKESASGVHKSVYTTNIENLGFDGMKAHFVTPEGEFDAHIHIPGEHNVYNAM
CCCEEEEECCCCCCCHHHHHEEECCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHH
AAAAVGLQLGLTIAEIKSGIEKAETIAGRTNFIKTHGMTVIDDCYNANPVSMKSSIEVLS
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHH
HAKGRTIAVLGDMGELGSDEKKLHHEVGEAVGENHIHTLFAAGELAKEYAAGAAEKSSDV
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCCCC
DIHYFEDRETMTQELLSYVKEGDTILVKASHFMEFPKVVEALSK
EEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDKYMKNLTFANIAKACEGTYIGDETLLDTTITGAVIDSRQVKEGYLFIPIKGERVDGH
CCHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHEEECCCCCCCCEEEEEEECCCCCCC
KFIPTVFEQGAAIVLSEHELTDPAGPYVLVESTTDAMKKLAAFYRKSLDIKVVGITGSVG
CCCHHHHCCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCC
KTSTKEMISSVLEQKYSVHKTAGNFNNEIGLPLTIFGIRESHQVAVLEMGISDFGEMHRL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHHH
STMSCPDVMVITNIGYCHLEFLGDRDGVLKAKTECFEHMMPDAVAVLNADDDKLATVQTV
CCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEE
NGKPAIYYGKESASGVHKSVYTTNIENLGFDGMKAHFVTPEGEFDAHIHIPGEHNVYNAM
CCCEEEEECCCCCCCHHHHHEEECCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHH
AAAAVGLQLGLTIAEIKSGIEKAETIAGRTNFIKTHGMTVIDDCYNANPVSMKSSIEVLS
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHH
HAKGRTIAVLGDMGELGSDEKKLHHEVGEAVGENHIHTLFAAGELAKEYAAGAAEKSSDV
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCCCC
DIHYFEDRETMTQELLSYVKEGDTILVKASHFMEFPKVVEALSK
EEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]