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The map label for this gene is ddl [H]

Identifier: 238925283

GI number: 238925283

Start: 2820759

End: 2821868

Strand: Reverse

Name: ddl [H]

Synonym: EUBREC_2937

Alternate gene names: 238925283

Gene position: 2821868-2820759 (Counterclockwise)

Preceding gene: 238925285

Following gene: 238925282

Centisome position: 81.8

GC content: 47.75

Gene sequence:

>1110_bases
TTGTGTTTAATTAAACCAAAGCATGATAATTCAATCAAAGAAAAGAGGACAAAAATGAACATCGTAGTACTTGCCGGAGG
GCTCAGCACAGAGAGAGATGTTTCCTTCAAGACAGGAAGCATGGTAGCTAAGGCGCTCAAAGAGAACGGCCACAAAGTTA
TATTATTGGATGTATTTATGGGATATAGTGACAGGGAGGAGGACCTGACAGGTATATTTGACCGCGCGGACGAGATAAGC
GTGCAGGTCAGCGATATTCCGACAGAGGCACCTGACCTCGCAAAGGTAAAGGCTTCCAGAAAGGACCAGTCACCTTGCTT
CTTCGGACCAAATGTCATCAAGATGTGTCAGATGGCAGACATTGTTTTCATGGCACTCCACGGAGAAAACGGAGAGAACG
GAAAGATACAGGCAGCATTTGACCTCTTCGGTGTCAAATATACAGGCAGCGATTATTTAAGCTCAGCCATCGCCATGAAC
AAGGAGACATCAAAGCAGTTCTTCCTTGCAAACGGTATCCCTACTCCAAAGGGCATTTCCATGACCAGAGCGACCAGACA
GGACGATATCACAAAGCTTGACCTGACACTTCCATGCGTGGTAAAGCCATGCTGCGGCGGTTCAAGCATCGGTGTCACTA
TCGTTAGGGATGCGGCAGAGTTCAAGGCGGCACTTGACGATGCCTTCAAGTGGGAAAATGAGCTGGTTATCGAGGAATTT
GTACAGGGCAGGGAGTTCTCTGTCGGCGTTATCGAGGGAAAGGCTCTTCCTATTATAGAGATTGCACCGAAGGAGGGCTT
CTACGATTATAAGAATAAATACAAGGCGGGCAGTACAGTAGAGACATGTCCGGCCGAGCTTCCTGAGCAGGTGACAAAGG
ATATGCAGCACTATGCAGAGGAGGTTGCGCGTGTTATCGGGCTTGACACATATTCACGCTCAGACTTTTTGCTCAACGAC
AAAAATGAGATGTTCTGCCTCGAGGCAAACACACTGCCGGGCATGACTCCGACCAGTCTTCTGCCACAGGAGGCAGCGGT
TATCGGTATGAGCTTTAATCAGCTCTGTGAGCATCTGATTGACATATCTTTGAAAAAATATGAGGTGTAG

Upstream 100 bases:

>100_bases
TTTTTGACCGGGCAACCGGTCTTTCTTTTTTGCACAAATGGTGTTATAATTTTGGAAAAATTATGTTGATTACTCATAGC
TTTAAAAAAGAAGTATTGCA

Downstream 100 bases:

>100_bases
CATGTCGGATAAGTACATGAAGAATCTTACATTTGCAAATATTGCAAAGGCCTGTGAGGGCACATACATAGGCGATGAGA
CACTGCTTGATACGACAATC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MCLIKPKHDNSIKEKRTKMNIVVLAGGLSTERDVSFKTGSMVAKALKENGHKVILLDVFMGYSDREEDLTGIFDRADEIS
VQVSDIPTEAPDLAKVKASRKDQSPCFFGPNVIKMCQMADIVFMALHGENGENGKIQAAFDLFGVKYTGSDYLSSAIAMN
KETSKQFFLANGIPTPKGISMTRATRQDDITKLDLTLPCVVKPCCGGSSIGVTIVRDAAEFKAALDDAFKWENELVIEEF
VQGREFSVGVIEGKALPIIEIAPKEGFYDYKNKYKAGSTVETCPAELPEQVTKDMQHYAEEVARVIGLDTYSRSDFLLND
KNEMFCLEANTLPGMTPTSLLPQEAAVIGMSFNQLCEHLIDISLKKYEV

Sequences:

>Translated_369_residues
MCLIKPKHDNSIKEKRTKMNIVVLAGGLSTERDVSFKTGSMVAKALKENGHKVILLDVFMGYSDREEDLTGIFDRADEIS
VQVSDIPTEAPDLAKVKASRKDQSPCFFGPNVIKMCQMADIVFMALHGENGENGKIQAAFDLFGVKYTGSDYLSSAIAMN
KETSKQFFLANGIPTPKGISMTRATRQDDITKLDLTLPCVVKPCCGGSSIGVTIVRDAAEFKAALDDAFKWENELVIEEF
VQGREFSVGVIEGKALPIIEIAPKEGFYDYKNKYKAGSTVETCPAELPEQVTKDMQHYAEEVARVIGLDTYSRSDFLLND
KNEMFCLEANTLPGMTPTSLLPQEAAVIGMSFNQLCEHLIDISLKKYEV
>Mature_369_residues
MCLIKPKHDNSIKEKRTKMNIVVLAGGLSTERDVSFKTGSMVAKALKENGHKVILLDVFMGYSDREEDLTGIFDRADEIS
VQVSDIPTEAPDLAKVKASRKDQSPCFFGPNVIKMCQMADIVFMALHGENGENGKIQAAFDLFGVKYTGSDYLSSAIAMN
KETSKQFFLANGIPTPKGISMTRATRQDDITKLDLTLPCVVKPCCGGSSIGVTIVRDAAEFKAALDDAFKWENELVIEEF
VQGREFSVGVIEGKALPIIEIAPKEGFYDYKNKYKAGSTVETCPAELPEQVTKDMQHYAEEVARVIGLDTYSRSDFLLND
KNEMFCLEANTLPGMTPTSLLPQEAAVIGMSFNQLCEHLIDISLKKYEV

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=350, Percent_Identity=31.1428571428571, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786579, Length=358, Percent_Identity=27.0949720670391, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40716; Mature: 40716

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLIKPKHDNSIKEKRTKMNIVVLAGGLSTERDVSFKTGSMVAKALKENGHKVILLDVFM
CEEECCCCCCCHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEC
GYSDREEDLTGIFDRADEISVQVSDIPTEAPDLAKVKASRKDQSPCFFGPNVIKMCQMAD
CCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCEECCHHHHHHHHHC
IVFMALHGENGENGKIQAAFDLFGVKYTGSDYLSSAIAMNKETSKQFFLANGIPTPKGIS
EEEEEEECCCCCCCEEEEEEEEEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
MTRATRQDDITKLDLTLPCVVKPCCGGSSIGVTIVRDAAEFKAALDDAFKWENELVIEEF
HHHCCCCCCCEEEEEEEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHH
VQGREFSVGVIEGKALPIIEIAPKEGFYDYKNKYKAGSTVETCPAELPEQVTKDMQHYAE
HCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCHHHCHHHHHHHHHHHHHHHHH
EVARVIGLDTYSRSDFLLNDKNEMFCLEANTLPGMTPTSLLPQEAAVIGMSFNQLCEHLI
HHHHHHCCCCCCCCCCEECCCCCEEEEECCCCCCCCCHHCCCCCCEEEECCHHHHHHHHH
DISLKKYEV
CCCHHCCCC
>Mature Secondary Structure
MCLIKPKHDNSIKEKRTKMNIVVLAGGLSTERDVSFKTGSMVAKALKENGHKVILLDVFM
CEEECCCCCCCHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEC
GYSDREEDLTGIFDRADEISVQVSDIPTEAPDLAKVKASRKDQSPCFFGPNVIKMCQMAD
CCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCEECCHHHHHHHHHC
IVFMALHGENGENGKIQAAFDLFGVKYTGSDYLSSAIAMNKETSKQFFLANGIPTPKGIS
EEEEEEECCCCCCCEEEEEEEEEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
MTRATRQDDITKLDLTLPCVVKPCCGGSSIGVTIVRDAAEFKAALDDAFKWENELVIEEF
HHHCCCCCCCEEEEEEEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHH
VQGREFSVGVIEGKALPIIEIAPKEGFYDYKNKYKAGSTVETCPAELPEQVTKDMQHYAE
HCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCHHHCHHHHHHHHHHHHHHHHH
EVARVIGLDTYSRSDFLLNDKNEMFCLEANTLPGMTPTSLLPQEAAVIGMSFNQLCEHLI
HHHHHHCCCCCCCCCCEECCCCCEEEEECCCCCCCCCHHCCCCCCEEEECCHHHHHHHHH
DISLKKYEV
CCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA