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The map label for this gene is hisS [H]

Identifier: 238925267

GI number: 238925267

Start: 2802012

End: 2803271

Strand: Reverse

Name: hisS [H]

Synonym: EUBREC_2921

Alternate gene names: 238925267

Gene position: 2803271-2802012 (Counterclockwise)

Preceding gene: 238925268

Following gene: 238925266

Centisome position: 81.26

GC content: 44.92

Gene sequence:

>1260_bases
ATGGCACTGAGCAAAAAGCCGGTCAACGGCATGAAGGATATACTACCTGCAGAGATGGAAATCCGTGATTATGTGACAAG
CGTGATCAAGGATACATATAGAAGCTTCGGATTTACACCGATAGAGACACCATGCATGGAGAATATAGCAAATCTCACAA
GCAAGCAGGGCGGAGAAAATGAGAAGCTGATCTTCAAGGTAATGAAGAGAGGCGAGAAGCTGAATCTGGAGACAGCAAAG
GAAGAGTCGGATGTAGTAGACTTTGGAATGCGCTATGACCTTACAGTGCCACTTTCAAGATATTATTCAAACAATGCCAA
CAACCTGCCATCGCCGTTTAAGGCACTTCAGATGGGCAGTGTGTGGAGAGCAGACAGACCTCAGAGAGGCCGCTACAGAC
AGTTTACACAGTGTGATATAGATATACTCGGAGAGCCAAGCAATCTGGCAGAGATAGAGCTGATTACAGCAACCACTACC
ACAATCGGAAGACTCGGTTTCGAGAACTTTGAGATAAGAATCAACGAGAGAAGAATCCTAAAGGCAATGGCTGCTTACAG
TGGTTTCGCTGAGGAGGACTACGACAGCGTATTTATCACACTCGATAAGATGGACAAGATAGGAGTGGAAGGTGTTGCAA
GTGAGCTTGCACAGGATGGCTACCCACAGGAAAATATAGACAAATATCTTGATCTGTTCAAGCTTTTGGAGCAGACAAGG
GATGTCACTGAGGGTGTAAATATACTTTCAGAAAAGCTCGGTGAATATTTAGACGAAGAAGTGGTTACAAATATGACAGA
GATAGCTACTGCTGTTAATGCCACAAAGGGAGCAAAGTTTGAGCTCGTATTTGACCCTACACTTGTTCGTGGAATGAGCT
ACTACACAGGAACTATTTTTGAGATATCAATGCCTGAGCTTGGCGCAGCCTGTGGCGGTGGCGGACGATATGACAAGATG
ATTGGAAAGTTCACCGGAAATGATGTACCGGCCTGTGGTTTTTCAATCGGTTTTGAGAGAATTATCCTGCTTCTCATGGA
GAGTGGCTTCAAGATTCCTGAGAGTCCAAAGAAGGTGGCTTATCTGGTTGAGAAGAAATATCCGGCAGACAAGCTTGTTG
ATGTCATGAACCAGGCAAAAGACGCAAGAGCAAACGGACAGCAGGTGCTTGTTGTGCGCATGAACAAGAACAAGAAATTC
CAGAAGGAGCAGCTTATGAAAGAGGGCTACGAGGAGTTTGTTGAGTTCTTTAACAGATAG

Upstream 100 bases:

>100_bases
ATACAGCAAAATCCCCTGTAACCCTTGACGATTGCAAAACCGAAAAGTATAATAGAATAGCTAATGAATTCAATATTTTA
ATATAGATGGAGGAAACCAA

Downstream 100 bases:

>100_bases
GAATCTGATAAGCAGCAGTTTAGCTGTGTAGCAGTGGTACCAAACAGTTATTAGAATAAATATATAGTAGAAATATAAGG
AGTATAAAATGGCAGAATCA

Product: histidine--tRNA ligase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MALSKKPVNGMKDILPAEMEIRDYVTSVIKDTYRSFGFTPIETPCMENIANLTSKQGGENEKLIFKVMKRGEKLNLETAK
EESDVVDFGMRYDLTVPLSRYYSNNANNLPSPFKALQMGSVWRADRPQRGRYRQFTQCDIDILGEPSNLAEIELITATTT
TIGRLGFENFEIRINERRILKAMAAYSGFAEEDYDSVFITLDKMDKIGVEGVASELAQDGYPQENIDKYLDLFKLLEQTR
DVTEGVNILSEKLGEYLDEEVVTNMTEIATAVNATKGAKFELVFDPTLVRGMSYYTGTIFEISMPELGAACGGGGRYDKM
IGKFTGNDVPACGFSIGFERIILLLMESGFKIPESPKKVAYLVEKKYPADKLVDVMNQAKDARANGQQVLVVRMNKNKKF
QKEQLMKEGYEEFVEFFNR

Sequences:

>Translated_419_residues
MALSKKPVNGMKDILPAEMEIRDYVTSVIKDTYRSFGFTPIETPCMENIANLTSKQGGENEKLIFKVMKRGEKLNLETAK
EESDVVDFGMRYDLTVPLSRYYSNNANNLPSPFKALQMGSVWRADRPQRGRYRQFTQCDIDILGEPSNLAEIELITATTT
TIGRLGFENFEIRINERRILKAMAAYSGFAEEDYDSVFITLDKMDKIGVEGVASELAQDGYPQENIDKYLDLFKLLEQTR
DVTEGVNILSEKLGEYLDEEVVTNMTEIATAVNATKGAKFELVFDPTLVRGMSYYTGTIFEISMPELGAACGGGGRYDKM
IGKFTGNDVPACGFSIGFERIILLLMESGFKIPESPKKVAYLVEKKYPADKLVDVMNQAKDARANGQQVLVVRMNKNKKF
QKEQLMKEGYEEFVEFFNR
>Mature_418_residues
ALSKKPVNGMKDILPAEMEIRDYVTSVIKDTYRSFGFTPIETPCMENIANLTSKQGGENEKLIFKVMKRGEKLNLETAKE
ESDVVDFGMRYDLTVPLSRYYSNNANNLPSPFKALQMGSVWRADRPQRGRYRQFTQCDIDILGEPSNLAEIELITATTTT
IGRLGFENFEIRINERRILKAMAAYSGFAEEDYDSVFITLDKMDKIGVEGVASELAQDGYPQENIDKYLDLFKLLEQTRD
VTEGVNILSEKLGEYLDEEVVTNMTEIATAVNATKGAKFELVFDPTLVRGMSYYTGTIFEISMPELGAACGGGGRYDKMI
GKFTGNDVPACGFSIGFERIILLLMESGFKIPESPKKVAYLVEKKYPADKLVDVMNQAKDARANGQQVLVVRMNKNKKFQ
KEQLMKEGYEEFVEFFNR

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=367, Percent_Identity=30.7901907356948, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI15029520, Length=371, Percent_Identity=28.8409703504043, Blast_Score=145, Evalue=9e-35,
Organism=Escherichia coli, GI1788861, Length=375, Percent_Identity=26.6666666666667, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71993686, Length=384, Percent_Identity=30.2083333333333, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71993693, Length=380, Percent_Identity=30.2631578947368, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6325290, Length=383, Percent_Identity=28.1984334203655, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24643061, Length=425, Percent_Identity=28.4705882352941, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24643059, Length=433, Percent_Identity=27.7136258660508, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47318; Mature: 47187

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSKKPVNGMKDILPAEMEIRDYVTSVIKDTYRSFGFTPIETPCMENIANLTSKQGGEN
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
EKLIFKVMKRGEKLNLETAKEESDVVDFGMRYDLTVPLSRYYSNNANNLPSPFKALQMGS
HHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEECCHHHHHCCCCCCCCCHHHHHHHCC
VWRADRPQRGRYRQFTQCDIDILGEPSNLAEIELITATTTTIGRLGFENFEIRINERRIL
CCCCCCCCCCCCCCHHHCCCEEECCCCCCCEEEEEEECHHHHHHCCCCCEEEEECHHHHH
KAMAAYSGFAEEDYDSVFITLDKMDKIGVEGVASELAQDGYPQENIDKYLDLFKLLEQTR
HHHHHHCCCCCCCCCEEEEEECCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
DVTEGVNILSEKLGEYLDEEVVTNMTEIATAVNATKGAKFELVFDPTLVRGMSYYTGTIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHCCHHHCCEEE
EISMPELGAACGGGGRYDKMIGKFTGNDVPACGFSIGFERIILLLMESGFKIPESPKKVA
EECCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
YLVEKKYPADKLVDVMNQAKDARANGQQVLVVRMNKNKKFQKEQLMKEGYEEFVEFFNR
HHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALSKKPVNGMKDILPAEMEIRDYVTSVIKDTYRSFGFTPIETPCMENIANLTSKQGGEN
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
EKLIFKVMKRGEKLNLETAKEESDVVDFGMRYDLTVPLSRYYSNNANNLPSPFKALQMGS
HHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEECCHHHHHCCCCCCCCCHHHHHHHCC
VWRADRPQRGRYRQFTQCDIDILGEPSNLAEIELITATTTTIGRLGFENFEIRINERRIL
CCCCCCCCCCCCCCHHHCCCEEECCCCCCCEEEEEEECHHHHHHCCCCCEEEEECHHHHH
KAMAAYSGFAEEDYDSVFITLDKMDKIGVEGVASELAQDGYPQENIDKYLDLFKLLEQTR
HHHHHHCCCCCCCCCEEEEEECCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
DVTEGVNILSEKLGEYLDEEVVTNMTEIATAVNATKGAKFELVFDPTLVRGMSYYTGTIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHCCHHHCCEEE
EISMPELGAACGGGGRYDKMIGKFTGNDVPACGFSIGFERIILLLMESGFKIPESPKKVA
EECCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
YLVEKKYPADKLVDVMNQAKDARANGQQVLVVRMNKNKKFQKEQLMKEGYEEFVEFFNR
HHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA