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The map label for this gene is aspS

Identifier: 238925266

GI number: 238925266

Start: 2800127

End: 2801923

Strand: Reverse

Name: aspS

Synonym: EUBREC_2920

Alternate gene names: 238925266

Gene position: 2801923-2800127 (Counterclockwise)

Preceding gene: 238925267

Following gene: 238925265

Centisome position: 81.22

GC content: 46.36

Gene sequence:

>1797_bases
ATGGCAGAATCAATGCAGGGCTTACATAGAAGCCATAGATGTACAGAGGTATCAAATGCCAATATCGGTGAGAAGGTCAC
CGTCATGGGCTGGGTACAGAAGAGAAGAAATTTAGGAAGTCTCATTTTCGTAGATTTAAGAGACAGAAGCGGAATACTTC
AGATAGTATTCGGTGAGGAAAATGTAGGTGCAGAGGGCTTTGAAAAGGCAGGCACCTTAAGAAGTGAGTTCGTCATCGCA
GTTGAGGGAACTGTTCAGAAGAGAACAGCTGCTGTCAATGAGAGCTTAAAGACAGGTGATATCGAGGTTATCGCTACATC
AATCAGAGTGCTTTCAGAGGCACAGACACCTCCATTCCATATTGAGGAGAATTCAAAGACCAGCGAGGACATCAGACTTA
AATACCGTTATCTGGATCTTCGCCGTCCTGATATCCAGAGAAATCTCATGCTCAGAAGCAATGTGCTTCGCGTGATGCGT
GACTTTATGGCAAACGAGGGATTTTTGGAGATAGAGACACCAATCCTTTGCAAGTCTACACCGGAGGGCGCCAGAGACTA
TCTCGTGCCAAGCCGTATACACCACGGACATTTTTATGCACTTCCTCAGTCACCACAGCTCTTTAAGCAGCTTCTGATGG
CTTCAGGTTATGACAGATATTTCCAGATTGCACGATGCTTCCGTGACGAGGATCTGCGTGCAGACAGACAGCCTGAGTTT
ACACAGGCCGATATGGAGCTTTCATTTGTAGATATCGATGATGTAATCGAGGTAAATGAGAGACTGTTAAAGCACCTTTT
TAAAGAGGTCATAAATGTGGATGTTGAGATTCCGTTTAAGCGTATGCCTTGGCAGGAGGCTATGGACAGATTTGGTTCTG
ACAAGCCTGATACACGCTTTGGTATGGAGCTTTGCGATGTTTCGGAAGTGGTAAAGGACTGTGGCTTTGGAGTATTCACA
GGAGCTCTTGAAAACGGTGGCTCTGTCCGTGGTATCAATGTAGAGGGACAGGCAAAGATGCCTCGTAAGAAGATTGACAA
GCTTGTAGAGCATGCAAAGGGCTGTGGAGCAAAGGGACTTGCTTATCTGTGCATCAATGAGGATGGCACATACAAGTCAT
CATTTGCCAAGTTTATGACAGAAGCTGAGCTTGATGCACTTGTTGCAAAGATGAACGGAAAGCCGGGAGATTTACTCCTT
TTCGCAGCAGACAAGAATAAAATCGTATGGAATGTACTTGGAGCGCTTCGTCTCATGCTCGGAGCTGAGCTTGGACTGAT
TGATGAGAACAAGTACAATTTCCTCTGGGTAACTGAGTTCCCACTCCTTGAGTGGTCTGATGAGGAAAACAGATTTATGG
CAATGCATCATCCATTCACCATGCCTATGGAGGAGGATTGGGACAAGATAGACTCAGATCCGGGCGCAGTTCGTGCAAAG
GCATATGATATCGTACTCAATGGTACAGAGCTCGGTGGAGGCTCAGTGCGTATCCATCAGGATGACATCCAGGAGAAGAT
GTTTGAGGTGCTTGGTTTCACAAAGGAGCGTGCACATGAGCAGTTTGGATTCCTGCTTGATGCATTCTCATATGGTGTAC
CGCCACATGCAGGACTTGCCTTCGGTGTTGACCGTATCTGCATGCATATGCTGCATACAGACAATATCAAGGAGGTTATT
GCATTCCCTAAGGTAAAGGACGCTTCGGACCTCATGTCAGAGGCACCCGGAACTGTAGATCCAAAGCAGCTTGAGGAGCT
TGGAATAGCTGTAGCTGCAGAGGAAGATGAGGAATAA

Upstream 100 bases:

>100_bases
TTTAACAGATAGGAATCTGATAAGCAGCAGTTTAGCTGTGTAGCAGTGGTACCAAACAGTTATTAGAATAAATATATAGT
AGAAATATAAGGAGTATAAA

Downstream 100 bases:

>100_bases
CACTATAATATGAATTTGAAAAAAAATATTTTCAATGCGATTACCATAATACTGCTCGCCGCACTTATTGCGGGGGCAGT
ATTGCTTAATAACAGGATAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MAESMQGLHRSHRCTEVSNANIGEKVTVMGWVQKRRNLGSLIFVDLRDRSGILQIVFGEENVGAEGFEKAGTLRSEFVIA
VEGTVQKRTAAVNESLKTGDIEVIATSIRVLSEAQTPPFHIEENSKTSEDIRLKYRYLDLRRPDIQRNLMLRSNVLRVMR
DFMANEGFLEIETPILCKSTPEGARDYLVPSRIHHGHFYALPQSPQLFKQLLMASGYDRYFQIARCFRDEDLRADRQPEF
TQADMELSFVDIDDVIEVNERLLKHLFKEVINVDVEIPFKRMPWQEAMDRFGSDKPDTRFGMELCDVSEVVKDCGFGVFT
GALENGGSVRGINVEGQAKMPRKKIDKLVEHAKGCGAKGLAYLCINEDGTYKSSFAKFMTEAELDALVAKMNGKPGDLLL
FAADKNKIVWNVLGALRLMLGAELGLIDENKYNFLWVTEFPLLEWSDEENRFMAMHHPFTMPMEEDWDKIDSDPGAVRAK
AYDIVLNGTELGGGSVRIHQDDIQEKMFEVLGFTKERAHEQFGFLLDAFSYGVPPHAGLAFGVDRICMHMLHTDNIKEVI
AFPKVKDASDLMSEAPGTVDPKQLEELGIAVAAEEDEE

Sequences:

>Translated_598_residues
MAESMQGLHRSHRCTEVSNANIGEKVTVMGWVQKRRNLGSLIFVDLRDRSGILQIVFGEENVGAEGFEKAGTLRSEFVIA
VEGTVQKRTAAVNESLKTGDIEVIATSIRVLSEAQTPPFHIEENSKTSEDIRLKYRYLDLRRPDIQRNLMLRSNVLRVMR
DFMANEGFLEIETPILCKSTPEGARDYLVPSRIHHGHFYALPQSPQLFKQLLMASGYDRYFQIARCFRDEDLRADRQPEF
TQADMELSFVDIDDVIEVNERLLKHLFKEVINVDVEIPFKRMPWQEAMDRFGSDKPDTRFGMELCDVSEVVKDCGFGVFT
GALENGGSVRGINVEGQAKMPRKKIDKLVEHAKGCGAKGLAYLCINEDGTYKSSFAKFMTEAELDALVAKMNGKPGDLLL
FAADKNKIVWNVLGALRLMLGAELGLIDENKYNFLWVTEFPLLEWSDEENRFMAMHHPFTMPMEEDWDKIDSDPGAVRAK
AYDIVLNGTELGGGSVRIHQDDIQEKMFEVLGFTKERAHEQFGFLLDAFSYGVPPHAGLAFGVDRICMHMLHTDNIKEVI
AFPKVKDASDLMSEAPGTVDPKQLEELGIAVAAEEDEE
>Mature_597_residues
AESMQGLHRSHRCTEVSNANIGEKVTVMGWVQKRRNLGSLIFVDLRDRSGILQIVFGEENVGAEGFEKAGTLRSEFVIAV
EGTVQKRTAAVNESLKTGDIEVIATSIRVLSEAQTPPFHIEENSKTSEDIRLKYRYLDLRRPDIQRNLMLRSNVLRVMRD
FMANEGFLEIETPILCKSTPEGARDYLVPSRIHHGHFYALPQSPQLFKQLLMASGYDRYFQIARCFRDEDLRADRQPEFT
QADMELSFVDIDDVIEVNERLLKHLFKEVINVDVEIPFKRMPWQEAMDRFGSDKPDTRFGMELCDVSEVVKDCGFGVFTG
ALENGGSVRGINVEGQAKMPRKKIDKLVEHAKGCGAKGLAYLCINEDGTYKSSFAKFMTEAELDALVAKMNGKPGDLLLF
AADKNKIVWNVLGALRLMLGAELGLIDENKYNFLWVTEFPLLEWSDEENRFMAMHHPFTMPMEEDWDKIDSDPGAVRAKA
YDIVLNGTELGGGSVRIHQDDIQEKMFEVLGFTKERAHEQFGFLLDAFSYGVPPHAGLAFGVDRICMHMLHTDNIKEVIA
FPKVKDASDLMSEAPGTVDPKQLEELGIAVAAEEDEE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=38.7312186978297, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI5031815, Length=291, Percent_Identity=27.8350515463918, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI194272210, Length=291, Percent_Identity=27.8350515463918, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI45439306, Length=322, Percent_Identity=25.1552795031056, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI4758762, Length=286, Percent_Identity=28.6713286713287, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI226491502, Length=254, Percent_Identity=24.8031496062992, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=49.8299319727891, Blast_Score=573, Evalue=1e-164,
Organism=Escherichia coli, GI1790571, Length=307, Percent_Identity=28.6644951140065, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1789256, Length=298, Percent_Identity=28.1879194630872, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=34.983498349835, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=307, Percent_Identity=26.7100977198697, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17535927, Length=307, Percent_Identity=26.7100977198697, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17535925, Length=307, Percent_Identity=26.7100977198697, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=26.6187050359712, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71984122, Length=289, Percent_Identity=26.9896193771626, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=624, Percent_Identity=32.8525641025641, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6323011, Length=276, Percent_Identity=27.8985507246377, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=344, Percent_Identity=25.5813953488372, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=318, Percent_Identity=24.2138364779874, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=265, Percent_Identity=24.1509433962264, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24584738, Length=612, Percent_Identity=36.7647058823529, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136276, Length=243, Percent_Identity=30.8641975308642, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=28.6274509803922, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=28.6274509803922, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19921528, Length=293, Percent_Identity=27.9863481228669, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_EUBR3 (C4ZI12)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002938783.1
- GeneID:   7963419
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_2920
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67491; Mature: 67360

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESMQGLHRSHRCTEVSNANIGEKVTVMGWVQKRRNLGSLIFVDLRDRSGILQIVFGEE
CCCHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCC
NVGAEGFEKAGTLRSEFVIAVEGTVQKRTAAVNESLKTGDIEVIATSIRVLSEAQTPPFH
CCCCCCHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEE
IEENSKTSEDIRLKYRYLDLRRPDIQRNLMLRSNVLRVMRDFMANEGFLEIETPILCKST
ECCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCC
PEGARDYLVPSRIHHGHFYALPQSPQLFKQLLMASGYDRYFQIARCFRDEDLRADRQPEF
CCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
TQADMELSFVDIDDVIEVNERLLKHLFKEVINVDVEIPFKRMPWQEAMDRFGSDKPDTRF
EECCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHCCCCCCCHH
GMELCDVSEVVKDCGFGVFTGALENGGSVRGINVEGQAKMPRKKIDKLVEHAKGCGAKGL
HHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCE
AYLCINEDGTYKSSFAKFMTEAELDALVAKMNGKPGDLLLFAADKNKIVWNVLGALRLML
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
GAELGLIDENKYNFLWVTEFPLLEWSDEENRFMAMHHPFTMPMEEDWDKIDSDPGAVRAK
CCCCCEEECCCCCEEEEEECCCEECCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCEEEE
AYDIVLNGTELGGGSVRIHQDDIQEKMFEVLGFTKERAHEQFGFLLDAFSYGVPPHAGLA
EEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
FGVDRICMHMLHTDNIKEVIAFPKVKDASDLMSEAPGTVDPKQLEELGIAVAAEEDEE
HHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHCCEEEEECCCCC
>Mature Secondary Structure 
AESMQGLHRSHRCTEVSNANIGEKVTVMGWVQKRRNLGSLIFVDLRDRSGILQIVFGEE
CCHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCC
NVGAEGFEKAGTLRSEFVIAVEGTVQKRTAAVNESLKTGDIEVIATSIRVLSEAQTPPFH
CCCCCCHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEE
IEENSKTSEDIRLKYRYLDLRRPDIQRNLMLRSNVLRVMRDFMANEGFLEIETPILCKST
ECCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCC
PEGARDYLVPSRIHHGHFYALPQSPQLFKQLLMASGYDRYFQIARCFRDEDLRADRQPEF
CCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
TQADMELSFVDIDDVIEVNERLLKHLFKEVINVDVEIPFKRMPWQEAMDRFGSDKPDTRF
EECCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHCCCCCCCHH
GMELCDVSEVVKDCGFGVFTGALENGGSVRGINVEGQAKMPRKKIDKLVEHAKGCGAKGL
HHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCE
AYLCINEDGTYKSSFAKFMTEAELDALVAKMNGKPGDLLLFAADKNKIVWNVLGALRLML
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
GAELGLIDENKYNFLWVTEFPLLEWSDEENRFMAMHHPFTMPMEEDWDKIDSDPGAVRAK
CCCCCEEECCCCCEEEEEECCCEECCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCEEEE
AYDIVLNGTELGGGSVRIHQDDIQEKMFEVLGFTKERAHEQFGFLLDAFSYGVPPHAGLA
EEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
FGVDRICMHMLHTDNIKEVIAFPKVKDASDLMSEAPGTVDPKQLEELGIAVAAEEDEE
HHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA