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The map label for this gene is leuO [H]

Identifier: 238921592

GI number: 238921592

Start: 3632301

End: 3633194

Strand: Reverse

Name: leuO [H]

Synonym: NT01EI_3745

Alternate gene names: 238921592

Gene position: 3633194-3632301 (Counterclockwise)

Preceding gene: 238921594

Following gene: 238921590

Centisome position: 95.3

GC content: 51.57

Gene sequence:

>894_bases
ATGCTCGATGTTGATGTGAGTGTTATTAGGACTTTTCTGACCGTGATGGATACGCGTAGTGTAACACTGGCTGCAGAGTT
GTTGGGCAGCTCTACAGCTTCGATTAGCCGCGCGTTGAAAAAAATGCGTGATGCATTTAACGACCCGTTGTTTATCCGGA
CCAAGCAAGGGTTAGAGCCGACAGCGTTAGCATACAATATTACGCCTAATCTGGCGCAGGCATTAAATAATATTCAGCGC
GCCGCGAATTTAACCAATGGTATTCACCAGGGCGATCGCCGTCAGACCCGATTAAAGCTCAGTATGTCCCCGTTGTTGGA
GTTTTATCTGACCCGTTTGCTGCAGCGTTACCATTACCCATTTGACAGCGTAGCGTTGATGACGGAAACCCATGATGGTG
ATTTAACCCGGGATATCAGTCGGCTACGCTCGCGGAAAATCGATATCAGCTTTACGCCGCAGAAATACGCAGACTGGATG
ATTACCAATGTGCCTGTATTCGATGTACAGCCCGCAGTGTTGTGCCGGGTGGGCCATCCCCGCATCGGCCCGGGGTTTAC
GCTGGAGCAGCTGGCGCAGGAGGATTACCTGAGCCTGACGATGTGGCCGTCGTTGTTTGAGGATGATGAGGTACTTAACC
CTCACCGGAAGGCGCCGATCTACTCCAGCTCATCGTTGCTGAATCTGATGATGGTGGCGGCCTCCAGCGATTATATCCTG
CTGTGTAGCAAGCAGTTTGCCCAGGAGTTTGCGCGTCTGGTGGAGGTTCAGGTATTGGATCACACCCTGGGGCGCTCCAC
GGGAACCATATATGCGCACTATCATAAAAGCCGGACGACCGATCCCTGCATTATCGAATTGATAGATATTCTACGCATCC
GTGGCAGTGACTAA

Upstream 100 bases:

>100_bases
CTGTTGCCAGCATAGGATAATTGCATAAAAAATATCCTGAATTTACAATGGCCGTGCCAGTATGCGGATCCTGCTGTAGT
GGCTGACCAAAGGTAAAAAT

Downstream 100 bases:

>100_bases
GAGCCGCTAACCGGTGCCAGCATGTCATTCCGCTGCCAAAACCCAGCGACTGGGGCAAGTCCTCCCACGGTATCCCAGTC
GTAAGGACAAACAGAATACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLDVDVSVIRTFLTVMDTRSVTLAAELLGSSTASISRALKKMRDAFNDPLFIRTKQGLEPTALAYNITPNLAQALNNIQR
AANLTNGIHQGDRRQTRLKLSMSPLLEFYLTRLLQRYHYPFDSVALMTETHDGDLTRDISRLRSRKIDISFTPQKYADWM
ITNVPVFDVQPAVLCRVGHPRIGPGFTLEQLAQEDYLSLTMWPSLFEDDEVLNPHRKAPIYSSSSLLNLMMVAASSDYIL
LCSKQFAQEFARLVEVQVLDHTLGRSTGTIYAHYHKSRTTDPCIIELIDILRIRGSD

Sequences:

>Translated_297_residues
MLDVDVSVIRTFLTVMDTRSVTLAAELLGSSTASISRALKKMRDAFNDPLFIRTKQGLEPTALAYNITPNLAQALNNIQR
AANLTNGIHQGDRRQTRLKLSMSPLLEFYLTRLLQRYHYPFDSVALMTETHDGDLTRDISRLRSRKIDISFTPQKYADWM
ITNVPVFDVQPAVLCRVGHPRIGPGFTLEQLAQEDYLSLTMWPSLFEDDEVLNPHRKAPIYSSSSLLNLMMVAASSDYIL
LCSKQFAQEFARLVEVQVLDHTLGRSTGTIYAHYHKSRTTDPCIIELIDILRIRGSD
>Mature_297_residues
MLDVDVSVIRTFLTVMDTRSVTLAAELLGSSTASISRALKKMRDAFNDPLFIRTKQGLEPTALAYNITPNLAQALNNIQR
AANLTNGIHQGDRRQTRLKLSMSPLLEFYLTRLLQRYHYPFDSVALMTETHDGDLTRDISRLRSRKIDISFTPQKYADWM
ITNVPVFDVQPAVLCRVGHPRIGPGFTLEQLAQEDYLSLTMWPSLFEDDEVLNPHRKAPIYSSSSLLNLMMVAASSDYIL
LCSKQFAQEFARLVEVQVLDHTLGRSTGTIYAHYHKSRTTDPCIIELIDILRIRGSD

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=281, Percent_Identity=23.4875444839858, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786820, Length=293, Percent_Identity=23.8907849829352, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081766, Length=299, Percent_Identity=22.742474916388, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790147, Length=195, Percent_Identity=25.6410256410256, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33663; Mature: 33663

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDVDVSVIRTFLTVMDTRSVTLAAELLGSSTASISRALKKMRDAFNDPLFIRTKQGLEP
CCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TALAYNITPNLAQALNNIQRAANLTNGIHQGDRRQTRLKLSMSPLLEFYLTRLLQRYHYP
CEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FDSVALMTETHDGDLTRDISRLRSRKIDISFTPQKYADWMITNVPVFDVQPAVLCRVGHP
CCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCHHHHCEEEECCCEECCCHHHEEECCCC
RIGPGFTLEQLAQEDYLSLTMWPSLFEDDEVLNPHRKAPIYSSSSLLNLMMVAASSDYIL
CCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
LCSKQFAQEFARLVEVQVLDHTLGRSTGTIYAHYHKSRTTDPCIIELIDILRIRGSD
EECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDVDVSVIRTFLTVMDTRSVTLAAELLGSSTASISRALKKMRDAFNDPLFIRTKQGLEP
CCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TALAYNITPNLAQALNNIQRAANLTNGIHQGDRRQTRLKLSMSPLLEFYLTRLLQRYHYP
CEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FDSVALMTETHDGDLTRDISRLRSRKIDISFTPQKYADWMITNVPVFDVQPAVLCRVGHP
CCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCHHHHCEEEECCCEECCCHHHEEECCCC
RIGPGFTLEQLAQEDYLSLTMWPSLFEDDEVLNPHRKAPIYSSSSLLNLMMVAASSDYIL
CCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
LCSKQFAQEFARLVEVQVLDHTLGRSTGTIYAHYHKSRTTDPCIIELIDILRIRGSD
EECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]