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The map label for this gene is fdhF [H]

Identifier: 238921137

GI number: 238921137

Start: 3156478

End: 3156897

Strand: Direct

Name: fdhF [H]

Synonym: NT01EI_3278

Alternate gene names: 238921137

Gene position: 3156478-3156897 (Clockwise)

Preceding gene: 238921136

Following gene: 238921138

Centisome position: 82.8

GC content: 57.14

Gene sequence:

>420_bases
ATGAAGAAAGTCATCACGGTCTGCCCTTATTGCGCCTCAGGTTGCAAAATCAACCTGGTGGTGGATAACGGCAGAATCAT
TCGTGCCGAAGGGGCGAATGGCGTGACGAACCAGGGCGAGCTGTGCCTGAAAGGGTACTATGGCTGGGATTTTATCCACG
ATACCAAAATCCTGACCCCACGCCTGAAGTCACCGATGATCCGCCGCGAACGCGGCGGTAAACTGGAAGCGGTTTCCTGG
GACGAGGCGATCGCCTTCGCCAGCTCCCGGCTGCTGGCGGTCAAAGAGAAGTACGGCCCGGATGCCATTATGACCACCGG
CTCATCACGCGGTCCCGGCAACGAAACCAACTACGTGATGCAAAAGTTCGCCCGTGCGGCTATCGGTACTAACAATATCG
ACTGCTGCGCACGCGTCTGA

Upstream 100 bases:

>100_bases
TGACAGGCGGGACGCACACCGATGCGCCAAGGCTCTCTGTAGATAGTTACGCCGGACGGGCACGCGACGTCCGGTGGTCA
TAAAAATAGGAGCAATGTTG

Downstream 100 bases:

>100_bases
CACGGCCCTTCGGTTGCAGGTCTGCAGCGATCGGTCGGTAACGGCGCCATGAGCAACTCCATCGTCGAACTTGAAGATAC
TGACTGCATTTTTGTCTTCG

Product: formate dehydrogenase H

Products: NA

Alternate protein names: Formate dehydrogenase-H subunit alpha; FDH-H; Formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide [H]

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MKKVITVCPYCASGCKINLVVDNGRIIRAEGANGVTNQGELCLKGYYGWDFIHDTKILTPRLKSPMIRRERGGKLEAVSW
DEAIAFASSRLLAVKEKYGPDAIMTTGSSRGPGNETNYVMQKFARAAIGTNNIDCCARV

Sequences:

>Translated_139_residues
MKKVITVCPYCASGCKINLVVDNGRIIRAEGANGVTNQGELCLKGYYGWDFIHDTKILTPRLKSPMIRRERGGKLEAVSW
DEAIAFASSRLLAVKEKYGPDAIMTTGSSRGPGNETNYVMQKFARAAIGTNNIDCCARV
>Mature_139_residues
MKKVITVCPYCASGCKINLVVDNGRIIRAEGANGVTNQGELCLKGYYGWDFIHDTKILTPRLKSPMIRRERGGKLEAVSW
DEAIAFASSRLLAVKEKYGPDAIMTTGSSRGPGNETNYVMQKFARAAIGTNNIDCCARV

Specific function: Decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=139, Percent_Identity=82.7338129496403, Blast_Score=253, Evalue=3e-69,
Organism=Escherichia coli, GI1788534, Length=138, Percent_Identity=30.4347826086957, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006478
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 15205; Mature: 15205

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVITVCPYCASGCKINLVVDNGRIIRAEGANGVTNQGELCLKGYYGWDFIHDTKILTP
CCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEEECCCCCEEECCEEECC
RLKSPMIRRERGGKLEAVSWDEAIAFASSRLLAVKEKYGPDAIMTTGSSRGPGNETNYVM
CCCCCCHHHHCCCEEEEECHHHHHHHHHCCEEEEECCCCCCEEEECCCCCCCCCCHHHHH
QKFARAAIGTNNIDCCARV
HHHHHHHCCCCCCCEEECC
>Mature Secondary Structure
MKKVITVCPYCASGCKINLVVDNGRIIRAEGANGVTNQGELCLKGYYGWDFIHDTKILTP
CCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEEECCCCCEEECCEEECC
RLKSPMIRRERGGKLEAVSWDEAIAFASSRLLAVKEKYGPDAIMTTGSSRGPGNETNYVM
CCCCCCHHHHCCCEEEEECHHHHHHHHHCCEEEEECCCCCCEEEECCCCCCCCCCHHHHH
QKFARAAIGTNNIDCCARV
HHHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2941757; 8265357; 9278503; 2211698; 9036855 [H]