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The map label for this gene is hycI [H]

Identifier: 238921136

GI number: 238921136

Start: 3155696

End: 3156175

Strand: Direct

Name: hycI [H]

Synonym: NT01EI_3277

Alternate gene names: 238921136

Gene position: 3155696-3156175 (Clockwise)

Preceding gene: 238921135

Following gene: 238921137

Centisome position: 82.78

GC content: 62.5

Gene sequence:

>480_bases
ATGGCTGACAATCTAATCCTGTGCGTAGGCAACAGCATGATGGGCGACGATGGCGCCGGCCCACTGCTGGCGGAGAAGCT
GTGCGGACACCCCATCCCCGGCTGGCAGGTCATCGACGGCGGCAGCAGTCCGGAAAGCTACGCCCACACCGTGCGCGAAC
TGCGCCCGCAGAAACTGCTGATCGTCGATGCGACGGAGATGGGGCTGGAGCCGGGTGAAATCCGCATTATCGACGAGCGC
GACATCGCCGAGCTGTTTATCGTCACCACCCATAACCTGCCGTTGAGCTACCTGATAGCCCAGCTGCGCGAGGACGTGGA
CGATATCACCTTTATCGGGATCCAACCGGCCATCGTCGCCTTCTACGCGCCGATGATGCAGCCGGTCAAAGAGGCGGTCG
AAACGCTGTATCAGCGCCTGCACCGCTGGGGAGACGACGGCGGCATTGCGCGCCTGACAGTGCCCTCATGCGAGATCTGA

Upstream 100 bases:

>100_bases
ACAATGACGCCCCCGCAGCAGGCGTGGAGCCGCAGCCTGATCGACGCGCTGCAGCTGATCGAGAATGAACCGACAATCTA
TCTGATGGTGAAACGACACA

Downstream 100 bases:

>100_bases
TGCGCCGCCTGACGGGGTGCCAAAATTGTGCGACAGTTTTGTCGAGTCCGTCGGCACTTTGATGCGGATCATCATGGGAT
TCCCACGCCAGCCTTTATAG

Product: hydrogenase 3 maturation protease

Products: NA

Alternate protein names: HycI protease [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MADNLILCVGNSMMGDDGAGPLLAEKLCGHPIPGWQVIDGGSSPESYAHTVRELRPQKLLIVDATEMGLEPGEIRIIDER
DIAELFIVTTHNLPLSYLIAQLREDVDDITFIGIQPAIVAFYAPMMQPVKEAVETLYQRLHRWGDDGGIARLTVPSCEI

Sequences:

>Translated_159_residues
MADNLILCVGNSMMGDDGAGPLLAEKLCGHPIPGWQVIDGGSSPESYAHTVRELRPQKLLIVDATEMGLEPGEIRIIDER
DIAELFIVTTHNLPLSYLIAQLREDVDDITFIGIQPAIVAFYAPMMQPVKEAVETLYQRLHRWGDDGGIARLTVPSCEI
>Mature_158_residues
ADNLILCVGNSMMGDDGAGPLLAEKLCGHPIPGWQVIDGGSSPESYAHTVRELRPQKLLIVDATEMGLEPGEIRIIDERD
IAELFIVTTHNLPLSYLIAQLREDVDDITFIGIQPAIVAFYAPMMQPVKEAVETLYQRLHRWGDDGGIARLTVPSCEI

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3 [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=151, Percent_Identity=65.5629139072848, Blast_Score=209, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: =3.4.23.51 [H]

Molecular weight: Translated: 17481; Mature: 17350

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNLILCVGNSMMGDDGAGPLLAEKLCGHPIPGWQVIDGGSSPESYAHTVRELRPQKLL
CCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEE
IVDATEMGLEPGEIRIIDERDIAELFIVTTHNLPLSYLIAQLREDVDDITFIGIQPAIVA
EEEHHHHCCCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCHHHEEEECCCHHHHH
FYAPMMQPVKEAVETLYQRLHRWGDDGGIARLTVPSCEI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
ADNLILCVGNSMMGDDGAGPLLAEKLCGHPIPGWQVIDGGSSPESYAHTVRELRPQKLL
CCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEE
IVDATEMGLEPGEIRIIDERDIAELFIVTTHNLPLSYLIAQLREDVDDITFIGIQPAIVA
EEEHHHHCCCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCHHHEEEECCCHHHHH
FYAPMMQPVKEAVETLYQRLHRWGDDGGIARLTVPSCEI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]