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The map label for this gene is hycG [H]

Identifier: 238921134

GI number: 238921134

Start: 3154439

End: 3155263

Strand: Direct

Name: hycG [H]

Synonym: NT01EI_3275

Alternate gene names: 238921134

Gene position: 3154439-3155263 (Clockwise)

Preceding gene: 238921133

Following gene: 238921135

Centisome position: 82.74

GC content: 65.09

Gene sequence:

>825_bases
ATGAGCCAGATCATCACTACCCCACCGGCGGCGGGCGCGTTAATCGGTCCGCGCGACGCCAACGGCCAGCCGGTGCCCAT
CACACTGGATGCGCAGGCGGCCCGCCTGAAGCAGACCCTGCTGAAGGACATCCGCCGCTCGGCCTATGTCTACCGCGTTG
ACTGCGGCGGCTGCAACGGCTGCGAAATTGAAATTTTCGCCGCCATCTCACCGCTGTTCGACGCGGAGCGCTTCGGCATC
AAGGTGGTTGCCTCGCCGCGCCACGCCGATATTCTGCTGTTTACCGGCGCGGTGACCCGTGCCATGCGCATTCCGGCGAT
CCGCGCCTACCAGTCAGCCCCCGACCCCAAAATCGTTATCTCCTACGGTGCCTGCGGTTGCAGCGGCGGCATCTTTCACG
ATCTGTACTGCGTCTGGGGCGGCACCGACAAAATCGTCCCGGTGGATGTCTATATTCCGGGCTGTCCGCCGACACCGGCG
GCAACCCTGTATGGCTTTGCCGTCGCCCTCGGCCTGCTGGATCAAAAGCTCAAGGCGCAGCACCACAGCCAGCAGGATGA
TGAGCAGGCGGCCATCCTGCACCCGGATATCCCGCAGACCCTGCGGGTGTTGATCGATCGCGAGGCCCGGCGCATGTCCG
GCTACCGCTATGGTCGCCAGCTGGCCGACAAGTTTATGGCCCTGCTGGCCAGCCGCGACGCCGCTACCCTGGAGCAACGC
CTAGACCGCTTCCTGGCGGCGGAAAACGATCCGCGCCTGAGCGAGGTAATGGGACGGCTGATGCAGGTGTGCAGCGATGC
CGCTCCCCGGGGAGACCACCGATGA

Upstream 100 bases:

>100_bases
GGGCCCAGTTTGAAACCTGTCCGGCGTGCAAGCGTCAACACAACATGATCCCGACGGCGCGCATTGCGCTGGGCCGTCAC
CTGATGCCGGAGGCGTCACA

Downstream 100 bases:

>100_bases
GCGAACGTGCATCCGCCTATTTTCCCGACCCGACGGTGGTGTTTCATATCCTCAACGCCAAGTTTGTCCAGCAGGCTGAA
GATGAAGCGGCAACGCCGCC

Product: hydrogenase-4 component I

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSQIITTPPAAGALIGPRDANGQPVPITLDAQAARLKQTLLKDIRRSAYVYRVDCGGCNGCEIEIFAAISPLFDAERFGI
KVVASPRHADILLFTGAVTRAMRIPAIRAYQSAPDPKIVISYGACGCSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPA
ATLYGFAVALGLLDQKLKAQHHSQQDDEQAAILHPDIPQTLRVLIDREARRMSGYRYGRQLADKFMALLASRDAATLEQR
LDRFLAAENDPRLSEVMGRLMQVCSDAAPRGDHR

Sequences:

>Translated_274_residues
MSQIITTPPAAGALIGPRDANGQPVPITLDAQAARLKQTLLKDIRRSAYVYRVDCGGCNGCEIEIFAAISPLFDAERFGI
KVVASPRHADILLFTGAVTRAMRIPAIRAYQSAPDPKIVISYGACGCSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPA
ATLYGFAVALGLLDQKLKAQHHSQQDDEQAAILHPDIPQTLRVLIDREARRMSGYRYGRQLADKFMALLASRDAATLEQR
LDRFLAAENDPRLSEVMGRLMQVCSDAAPRGDHR
>Mature_273_residues
SQIITTPPAAGALIGPRDANGQPVPITLDAQAARLKQTLLKDIRRSAYVYRVDCGGCNGCEIEIFAAISPLFDAERFGIK
VVASPRHADILLFTGAVTRAMRIPAIRAYQSAPDPKIVISYGACGCSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAA
TLYGFAVALGLLDQKLKAQHHSQQDDEQAAILHPDIPQTLRVLIDREARRMSGYRYGRQLADKFMALLASRDAATLEQRL
DRFLAAENDPRLSEVMGRLMQVCSDAAPRGDHR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=129, Percent_Identity=41.8604651162791, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789074, Length=257, Percent_Identity=70.8171206225681, Blast_Score=376, Evalue=1e-106,
Organism=Escherichia coli, GI1788834, Length=238, Percent_Identity=63.0252100840336, Blast_Score=313, Evalue=9e-87,
Organism=Escherichia coli, GI1788624, Length=127, Percent_Identity=40.9448818897638, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=128, Percent_Identity=39.84375, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI18859983, Length=110, Percent_Identity=43.6363636363636, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24642371, Length=110, Percent_Identity=43.6363636363636, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24651058, Length=106, Percent_Identity=46.2264150943396, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 29828; Mature: 29697

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIITTPPAAGALIGPRDANGQPVPITLDAQAARLKQTLLKDIRRSAYVYRVDCGGCNG
CCCCCCCCCCCCCEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCEEEEEEECCCCCC
CEIEIFAAISPLFDAERFGIKVVASPRHADILLFTGAVTRAMRIPAIRAYQSAPDPKIVI
CCEEEEEECCCHHCHHHCCEEEEECCCCCEEEEEEHHHHHHHHCCHHHHHCCCCCCEEEE
SYGACGCSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAVALGLLDQKLKAQ
EECCCCCCCCHHEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
HHSQQDDEQAAILHPDIPQTLRVLIDREARRMSGYRYGRQLADKFMALLASRDAATLEQR
HCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LDRFLAAENDPRLSEVMGRLMQVCSDAAPRGDHR
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQIITTPPAAGALIGPRDANGQPVPITLDAQAARLKQTLLKDIRRSAYVYRVDCGGCNG
CCCCCCCCCCCCEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCEEEEEEECCCCCC
CEIEIFAAISPLFDAERFGIKVVASPRHADILLFTGAVTRAMRIPAIRAYQSAPDPKIVI
CCEEEEEECCCHHCHHHCCEEEEECCCCCEEEEEEHHHHHHHHCCHHHHHCCCCCCEEEE
SYGACGCSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAVALGLLDQKLKAQ
EECCCCCCCCHHEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
HHSQQDDEQAAILHPDIPQTLRVLIDREARRMSGYRYGRQLADKFMALLASRDAATLEQR
HCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LDRFLAAENDPRLSEVMGRLMQVCSDAAPRGDHR
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]