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The map label for this gene is hycF [H]

Identifier: 238921133

GI number: 238921133

Start: 3153885

End: 3154442

Strand: Direct

Name: hycF [H]

Synonym: NT01EI_3274

Alternate gene names: 238921133

Gene position: 3153885-3154442 (Clockwise)

Preceding gene: 238921132

Following gene: 238921134

Centisome position: 82.73

GC content: 65.95

Gene sequence:

>558_bases
ATGCTGAAACTGTTAAAAACTGCCCTGCGTCATCGCCATGCTACCGTCGCCTATCCGGCGCGTCCGCTGGATGTCGACGC
CAACTTCCGCGGCAAGCCGCAGTATGATGCCCAGCAGTGCATCGCCTGCGGTGCCTGCACCGCTGCCTGCCCGGCCAACG
CCCTGACCATGCAGACCGACGCCGCCAGCGGCGTACGGACCTGGGCACTCAATCTGGGGCGCTGCATCTTCTGCGCCCGC
TGTGAGGAAGTTTGCCCCACCGCCGCCATCGTACTGTCGTCGGAGTTTGAGCTGGCGGTGTGGCGCAAAGAGGATCTGCA
GCAGCGCGCCGAGTTCCGCGTCTGCCGCTGCATCGGGTGCGGCGCGCCTTACGCCGCGCAGAAAGAGATCGACTACGCCA
TGGCCCTGCTGGTCCAGGCCGGTCACCTGACGGCCGAACAGGCCGAGGCGCGCCGGGCCCAGTTTGAAACCTGTCCGGCG
TGCAAGCGTCAACACAACATGATCCCGACGGCGCGCATTGCGCTGGGCCGTCACCTGATGCCGGAGGCGTCACAATGA

Upstream 100 bases:

>100_bases
AACCGACGCAAATCGGTCACCGTGCCCTATAAGGAGATCGAGCGCTATGGACTGGAGCGCAAGGACTCACCGCTGAAGTG
ATCGCCGGAGACGAGAGACT

Downstream 100 bases:

>100_bases
GCCAGATCATCACTACCCCACCGGCGGCGGGCGCGTTAATCGGTCCGCGCGACGCCAACGGCCAGCCGGTGCCCATCACA
CTGGATGCGCAGGCGGCCCG

Product: formate hydrogenlyase complex iron-sulfur subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MLKLLKTALRHRHATVAYPARPLDVDANFRGKPQYDAQQCIACGACTAACPANALTMQTDAASGVRTWALNLGRCIFCAR
CEEVCPTAAIVLSSEFELAVWRKEDLQQRAEFRVCRCIGCGAPYAAQKEIDYAMALLVQAGHLTAEQAEARRAQFETCPA
CKRQHNMIPTARIALGRHLMPEASQ

Sequences:

>Translated_185_residues
MLKLLKTALRHRHATVAYPARPLDVDANFRGKPQYDAQQCIACGACTAACPANALTMQTDAASGVRTWALNLGRCIFCAR
CEEVCPTAAIVLSSEFELAVWRKEDLQQRAEFRVCRCIGCGAPYAAQKEIDYAMALLVQAGHLTAEQAEARRAQFETCPA
CKRQHNMIPTARIALGRHLMPEASQ
>Mature_185_residues
MLKLLKTALRHRHATVAYPARPLDVDANFRGKPQYDAQQCIACGACTAACPANALTMQTDAASGVRTWALNLGRCIFCAR
CEEVCPTAAIVLSSEFELAVWRKEDLQQRAEFRVCRCIGCGAPYAAQKEIDYAMALLVQAGHLTAEQAEARRAQFETCPA
CKRQHNMIPTARIALGRHLMPEASQ

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=140, Percent_Identity=31.4285714285714, Blast_Score=64, Evalue=6e-11,
Organism=Escherichia coli, GI1789075, Length=179, Percent_Identity=55.3072625698324, Blast_Score=213, Evalue=6e-57,
Organism=Escherichia coli, GI1788833, Length=168, Percent_Identity=54.1666666666667, Blast_Score=137, Evalue=3e-34,
Organism=Escherichia coli, GI1788617, Length=110, Percent_Identity=32.7272727272727, Blast_Score=72, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17555194, Length=107, Percent_Identity=32.7102803738318, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17864306, Length=111, Percent_Identity=34.2342342342342, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20337; Mature: 20337

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
7.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLLKTALRHRHATVAYPARPLDVDANFRGKPQYDAQQCIACGACTAACPANALTMQTD
CHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHCCCCCEEEECC
AASGVRTWALNLGRCIFCARCEEVCPTAAIVLSSEFELAVWRKEDLQQRAEFRVCRCIGC
CCCCHHHHHHHHCHHHHHHHHHHHCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCC
GAPYAAQKEIDYAMALLVQAGHLTAEQAEARRAQFETCPACKRQHNMIPTARIALGRHLM
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHCCCCC
PEASQ
CCCCC
>Mature Secondary Structure
MLKLLKTALRHRHATVAYPARPLDVDANFRGKPQYDAQQCIACGACTAACPANALTMQTD
CHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHCCCCCEEEECC
AASGVRTWALNLGRCIFCARCEEVCPTAAIVLSSEFELAVWRKEDLQQRAEFRVCRCIGC
CCCCHHHHHHHHCHHHHHHHHHHHCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCC
GAPYAAQKEIDYAMALLVQAGHLTAEQAEARRAQFETCPACKRQHNMIPTARIALGRHLM
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHCCCCC
PEASQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]