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The map label for this gene is ascG [H]

Identifier: 238921128

GI number: 238921128

Start: 3147429

End: 3148439

Strand: Direct

Name: ascG [H]

Synonym: NT01EI_3269

Alternate gene names: 238921128

Gene position: 3147429-3148439 (Clockwise)

Preceding gene: 238921127

Following gene: 238921129

Centisome position: 82.56

GC content: 63.9

Gene sequence:

>1011_bases
ATGATCACCATGCTGGACGTTGCACGCCGCGCAGGCGTATCCAAAGCGACGGTATCCCGCGTGCTCAATGCCACCGGGCA
GGTCAGACAAAGCACCCGCAGCGCCGTATTCTGCGCGATGGAGGAGCTGGGCTACCGCCCCAACCTGCTGGCCCAGGCAC
TGGCCAACCAGACCTCCAACACCCTGGGACTGGTGATCTCCCACTTTGATGGTCCCTACTTTGGCCGCCTGCTGCGCCAG
GCCGCCGCACAGGTCGAAGCCAGCGGCAAACAGCTGATCGTGACCGACGGCCACGATACGCCCGACGGCGAACGCCAGGC
GGTGCAGATGCTCAGCGATCGGCGCTGCGATGCCATCATTTTGTATACCCGCTTTATGGCCGAGACCGATCTGATGAAGC
TGGTATCGCAGTATCCCTCTTTGGTGGTGATCAACCGTGCGCTGCCGCAGGCAGCACAGCGCTGTATCCTCTTTGAACAG
CGCGCGGCAGCGCGCCAGGCGGTGGAGTATCTAATCGAACGTGGCCATCGCCGCATCGCCTGTATCACCCTGACCATCGA
TACCCCGACCGGACAGGCACGCCTGCAGGGCTACCATGACGCACTGCAGGCCCATGCACTCAGTGCCTCCCCCCAGGACG
TTGCCCACGGCAATAACAGCGTGGAGGGCGGATACCACGCCTGCCTGCGTCTGCTGGAGAACCACGATGCGCCGTTCAGC
GCCCTGTTCTGCTGCAACGATGATATGGCCATCGGCGCCCTTCGCGCCCTGCGCCAAGCGGGAATACGCGTCCCACAGGA
GGTCTCTCTGTTCGGCTTTGACGACCAGCCCATCGCCGCCTACCTGATGCCTGCGCTCTCCACGGTCTACCTGCCCATCG
ATGCCATGATCGCCGCCGCGATTCAACAGGCTCTGCGTCTGAGCCGCGGTGACCCCGTCGACACTCTGCCACCCTTTCGC
GGCGAAATGCGTCTGCGTGAGTCGGTGGCGGACGCCCCCGCGGTGCAGTAA

Upstream 100 bases:

>100_bases
TATGTTCAGTCCCACGCTGGTATGCCGCCATTCCGTCTCTCCCCTCCGGGCGCTGTGATACACTGTTGTACGCCATCGCC
ATCAAGGGGGATAAGGAACC

Downstream 100 bases:

>100_bases
TGCATCGTCATAAGTGTCGATATGCCGCATCCGCTGTCGACATCGTCACCTTGTCGATCGATGTCGACAGCGTCATTTCA
TAGCAATCAATTGATTTTAA

Product: HTH-type transcriptional regulator AscG

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MITMLDVARRAGVSKATVSRVLNATGQVRQSTRSAVFCAMEELGYRPNLLAQALANQTSNTLGLVISHFDGPYFGRLLRQ
AAAQVEASGKQLIVTDGHDTPDGERQAVQMLSDRRCDAIILYTRFMAETDLMKLVSQYPSLVVINRALPQAAQRCILFEQ
RAAARQAVEYLIERGHRRIACITLTIDTPTGQARLQGYHDALQAHALSASPQDVAHGNNSVEGGYHACLRLLENHDAPFS
ALFCCNDDMAIGALRALRQAGIRVPQEVSLFGFDDQPIAAYLMPALSTVYLPIDAMIAAAIQQALRLSRGDPVDTLPPFR
GEMRLRESVADAPAVQ

Sequences:

>Translated_336_residues
MITMLDVARRAGVSKATVSRVLNATGQVRQSTRSAVFCAMEELGYRPNLLAQALANQTSNTLGLVISHFDGPYFGRLLRQ
AAAQVEASGKQLIVTDGHDTPDGERQAVQMLSDRRCDAIILYTRFMAETDLMKLVSQYPSLVVINRALPQAAQRCILFEQ
RAAARQAVEYLIERGHRRIACITLTIDTPTGQARLQGYHDALQAHALSASPQDVAHGNNSVEGGYHACLRLLENHDAPFS
ALFCCNDDMAIGALRALRQAGIRVPQEVSLFGFDDQPIAAYLMPALSTVYLPIDAMIAAAIQQALRLSRGDPVDTLPPFR
GEMRLRESVADAPAVQ
>Mature_336_residues
MITMLDVARRAGVSKATVSRVLNATGQVRQSTRSAVFCAMEELGYRPNLLAQALANQTSNTLGLVISHFDGPYFGRLLRQ
AAAQVEASGKQLIVTDGHDTPDGERQAVQMLSDRRCDAIILYTRFMAETDLMKLVSQYPSLVVINRALPQAAQRCILFEQ
RAAARQAVEYLIERGHRRIACITLTIDTPTGQARLQGYHDALQAHALSASPQDVAHGNNSVEGGYHACLRLLENHDAPFS
ALFCCNDDMAIGALRALRQAGIRVPQEVSLFGFDDQPIAAYLMPALSTVYLPIDAMIAAAIQQALRLSRGDPVDTLPPFR
GEMRLRESVADAPAVQ

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=43.1137724550898, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=40.2027027027027, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1788474, Length=311, Percent_Identity=36.9774919614148, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=31.6715542521994, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1790194, Length=303, Percent_Identity=31.023102310231, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790369, Length=290, Percent_Identity=30.6896551724138, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=27.1293375394322, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=28.8025889967638, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1786540, Length=316, Percent_Identity=27.8481012658228, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790715, Length=297, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787906, Length=304, Percent_Identity=29.6052631578947, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790689, Length=289, Percent_Identity=26.2975778546713, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789456, Length=336, Percent_Identity=24.702380952381, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786268, Length=285, Percent_Identity=25.9649122807018, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790192, Length=281, Percent_Identity=24.9110320284698, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36670; Mature: 36670

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMLDVARRAGVSKATVSRVLNATGQVRQSTRSAVFCAMEELGYRPNLLAQALANQTSN
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
TLGLVISHFDGPYFGRLLRQAAAQVEASGKQLIVTDGHDTPDGERQAVQMLSDRRCDAII
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEE
LYTRFMAETDLMKLVSQYPSLVVINRALPQAAQRCILFEQRAAARQAVEYLIERGHRRIA
EHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
CITLTIDTPTGQARLQGYHDALQAHALSASPQDVAHGNNSVEGGYHACLRLLENHDAPFS
EEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCE
ALFCCNDDMAIGALRALRQAGIRVPQEVSLFGFDDQPIAAYLMPALSTVYLPIDAMIAAA
EEEEECCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IQQALRLSRGDPVDTLPPFRGEMRLRESVADAPAVQ
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITMLDVARRAGVSKATVSRVLNATGQVRQSTRSAVFCAMEELGYRPNLLAQALANQTSN
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
TLGLVISHFDGPYFGRLLRQAAAQVEASGKQLIVTDGHDTPDGERQAVQMLSDRRCDAII
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEE
LYTRFMAETDLMKLVSQYPSLVVINRALPQAAQRCILFEQRAAARQAVEYLIERGHRRIA
EHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
CITLTIDTPTGQARLQGYHDALQAHALSASPQDVAHGNNSVEGGYHACLRLLENHDAPFS
EEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCE
ALFCCNDDMAIGALRALRQAGIRVPQEVSLFGFDDQPIAAYLMPALSTVYLPIDAMIAAA
EEEEECCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IQQALRLSRGDPVDTLPPFRGEMRLRESVADAPAVQ
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]