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The map label for this gene is galR [H]

Identifier: 238921127

GI number: 238921127

Start: 3146379

End: 3147386

Strand: Direct

Name: galR [H]

Synonym: NT01EI_3268

Alternate gene names: 238921127

Gene position: 3146379-3147386 (Clockwise)

Preceding gene: 238921122

Following gene: 238921128

Centisome position: 82.53

GC content: 61.31

Gene sequence:

>1008_bases
ATGGCTACGATCAAAGATGTTGCCAGGCTTGCCGGCGTATCGGTAGCCACCGTCTCCCGGGTTATCAATAACTCCCCCAA
GGCCAGCGAAGGCTCCCGCACTGCGGTGCACGCCGCCATGGCACAGCTGCGTTATCACCCAAACGCCAACGCGCGGGCAC
TGGCTCAGCAGTCGACAGAGACCCTGGGACTGGTGGTCTGTGACGTCTCCGATCCGTTCTTTGGCGCCATGGTCAAAGCG
GTCGAGCAGATAGCCTACGCCACCGGCAACTTTCTGTTGATCGGCAATGGATACCATGAACAGCATAAAGAGCGGCAAGC
CATCGAACAGCTGATCCGCCACCGCTGCGCGGCGTTGGTAGTCCATGCCAAGACCATCCCCGACGATGAGCTGGCGGCAC
TGATGCAGCAGATCCCCGGTATGGTACTGATCAACCGGACTCTGCCCGGCTATGAACAGCGCTGCGTCGCACTGGACGAC
CGCTATGGCGGCTGGCTGGCGACACGCCATCTGATCCAGCAGGGGCACCGGCATATCGCCTGCATCTGCTCCAACCATGA
GATCTCCGATGCCCACGATCGCCTGCAGGGGTATCTGGAAGCCATGCGCCAGTACGATCAGCCGATCGATGAACGGCTGA
TCGTCCATGCCTCACCCGATGAGCTCGGCGGAGAGCTGGCGATGACCGAACTGCTGGGGCATGGTCGTAAATTCAGTGCA
GTCACCTGCTATAACGATCCCATGGCTGCCGGCGCGCTATCGGTGCTGAATGACAACGGCATTGAAGTGCCGGGCGCGGT
CTCCCTGATCGGCTTTGACGACGTACTGATCTCACGCTACCTGCGCCCGCGTCTAACCACCATCCGCTATCCGGTGGTCG
CCATGGCGACCCAGGCGGCAGAGCTGGCGTTGGCACTGGCCGCAGGGAAAACGCCGCCAGAAACCACCAATATGTTCAGT
CCCACGCTGGTATGCCGCCATTCCGTCTCTCCCCTCCGGGCGCTGTGA

Upstream 100 bases:

>100_bases
CATATTAGAGGCTCAAACGCCTATCCCCGGCTTGCAGGCGCGGCGCTTTTCCGCAACACTGGTGTAAACGTTTACGGTTT
GCAGTTGGACACACAAGAAA

Downstream 100 bases:

>100_bases
TACACTGTTGTACGCCATCGCCATCAAGGGGGATAAGGAACCATGATCACCATGCTGGACGTTGCACGCCGCGCAGGCGT
ATCCAAAGCGACGGTATCCC

Product: DNA-binding transcriptional regulator GalR

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MATIKDVARLAGVSVATVSRVINNSPKASEGSRTAVHAAMAQLRYHPNANARALAQQSTETLGLVVCDVSDPFFGAMVKA
VEQIAYATGNFLLIGNGYHEQHKERQAIEQLIRHRCAALVVHAKTIPDDELAALMQQIPGMVLINRTLPGYEQRCVALDD
RYGGWLATRHLIQQGHRHIACICSNHEISDAHDRLQGYLEAMRQYDQPIDERLIVHASPDELGGELAMTELLGHGRKFSA
VTCYNDPMAAGALSVLNDNGIEVPGAVSLIGFDDVLISRYLRPRLTTIRYPVVAMATQAAELALALAAGKTPPETTNMFS
PTLVCRHSVSPLRAL

Sequences:

>Translated_335_residues
MATIKDVARLAGVSVATVSRVINNSPKASEGSRTAVHAAMAQLRYHPNANARALAQQSTETLGLVVCDVSDPFFGAMVKA
VEQIAYATGNFLLIGNGYHEQHKERQAIEQLIRHRCAALVVHAKTIPDDELAALMQQIPGMVLINRTLPGYEQRCVALDD
RYGGWLATRHLIQQGHRHIACICSNHEISDAHDRLQGYLEAMRQYDQPIDERLIVHASPDELGGELAMTELLGHGRKFSA
VTCYNDPMAAGALSVLNDNGIEVPGAVSLIGFDDVLISRYLRPRLTTIRYPVVAMATQAAELALALAAGKTPPETTNMFS
PTLVCRHSVSPLRAL
>Mature_334_residues
ATIKDVARLAGVSVATVSRVINNSPKASEGSRTAVHAAMAQLRYHPNANARALAQQSTETLGLVVCDVSDPFFGAMVKAV
EQIAYATGNFLLIGNGYHEQHKERQAIEQLIRHRCAALVVHAKTIPDDELAALMQQIPGMVLINRTLPGYEQRCVALDDR
YGGWLATRHLIQQGHRHIACICSNHEISDAHDRLQGYLEAMRQYDQPIDERLIVHASPDELGGELAMTELLGHGRKFSAV
TCYNDPMAAGALSVLNDNGIEVPGAVSLIGFDDVLISRYLRPRLTTIRYPVVAMATQAAELALALAAGKTPPETTNMFSP
TLVCRHSVSPLRAL

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=77.5757575757576, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI1788474, Length=330, Percent_Identity=55.1515151515151, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=34.3195266272189, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=35, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=32.02416918429, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=31.8181818181818, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=25.3918495297806, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787906, Length=316, Percent_Identity=27.2151898734177, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786540, Length=319, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=24.203821656051, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=23.8244514106583, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=26.605504587156, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786268, Length=128, Percent_Identity=33.59375, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36444; Mature: 36313

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVARLAGVSVATVSRVINNSPKASEGSRTAVHAAMAQLRYHPNANARALAQQSTE
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
TLGLVVCDVSDPFFGAMVKAVEQIAYATGNFLLIGNGYHEQHKERQAIEQLIRHRCAALV
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
VHAKTIPDDELAALMQQIPGMVLINRTLPGYEQRCVALDDRYGGWLATRHLIQQGHRHIA
HEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHEEEECCCCCHHHHHHHHHHHCCCEEE
CICSNHEISDAHDRLQGYLEAMRQYDQPIDERLIVHASPDELGGELAMTELLGHGRKFSA
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHCCHHHHHHHHCCCCCEEE
VTCYNDPMAAGALSVLNDNGIEVPGAVSLIGFDDVLISRYLRPRLTTIRYPVVAMATQAA
EEEECCCHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELALALAAGKTPPETTNMFSPTLVCRHSVSPLRAL
HHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHCC
>Mature Secondary Structure 
ATIKDVARLAGVSVATVSRVINNSPKASEGSRTAVHAAMAQLRYHPNANARALAQQSTE
CCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
TLGLVVCDVSDPFFGAMVKAVEQIAYATGNFLLIGNGYHEQHKERQAIEQLIRHRCAALV
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
VHAKTIPDDELAALMQQIPGMVLINRTLPGYEQRCVALDDRYGGWLATRHLIQQGHRHIA
HEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHEEEECCCCCHHHHHHHHHHHCCCEEE
CICSNHEISDAHDRLQGYLEAMRQYDQPIDERLIVHASPDELGGELAMTELLGHGRKFSA
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHCCHHHHHHHHCCCCCEEE
VTCYNDPMAAGALSVLNDNGIEVPGAVSLIGFDDVLISRYLRPRLTTIRYPVVAMATQAA
EEEECCCHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELALALAAGKTPPETTNMFSPTLVCRHSVSPLRAL
HHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]