Click here to switch to the map view.

The map label for this gene is hycB [H]

Identifier: 238921129

GI number: 238921129

Start: 3148626

End: 3149240

Strand: Direct

Name: hycB [H]

Synonym: NT01EI_3270

Alternate gene names: 238921129

Gene position: 3148626-3149240 (Clockwise)

Preceding gene: 238921128

Following gene: 238921130

Centisome position: 82.59

GC content: 66.5

Gene sequence:

>615_bases
ATGAACCGCTTTGTGATTGCGGATCCCCGACTGTGCATCGGGTGCAATACCTGCATGGCCGCCTGCTCGCAGGAGCATCA
GGCGCAGGGCCTGCAGGCACAGCCGCGCCTGACGCTGGTCAAGACCAGCCACGAGTCGGCGCCGCAGATGTGTCATCACT
GCGAAGATGCCCCCTGCGCCCTGGTCTGCCCGGTCAATGCCATTACCCGCCAGGATGGCGCTATTCAGCTCAATGAGAGC
CTGTGCGTCGGCTGCAAGCTGTGCGGCATCGCCTGCCCCTTCGGCGCCATCACCCTGGGCGGCAGCAAACCGCTGCATAT
TCCCGCCAACAGCAATACGCCGCTGGCGCCGCCTGCGCCCCCGGCCCCCCTCAGCGTCGGCCCGTTCCTGGACTGGTATC
CCGGCATCCGCAGCATCGCGGTGAAGTGCGATCTGTGCCAGTTTAGCGACGCCGGGCCGGCCTGTGTCCGCGCCTGCCCA
ACCCACGCCATCGTACTGGTGGATGAGCGGCGCCTGGAGGATGCCAGCGCCGCCAAACGCCTGAACGTACTCGATGCCCA
GCGCGCCGACCTGCTGGCTCTCGCTCGCTTATCCGGGGGAAATGACGCCCTATGA

Upstream 100 bases:

>100_bases
TCAATTGATTTTAATTAATAAAAAATGAAATAATTCACTTGGCACGCTTTGTGCTTATCAGGGGCGGATCGTTTTTATAG
TCTGTCTTTGAGGTCATAAC

Downstream 100 bases:

>100_bases
GTATTCTGCAGCTGTTTAATCTGATGCTGGCGCTGTATCTGTGCAGCGCCATCATCGCGCTGGCATTCAGCGGTATCGGC
CGACTGGGCGCCCACATCGC

Product: 4Fe-4S binding domain protein

Products: Oxidized ferredoxin; CO2; H2 [C]

Alternate protein names: FHL subunit 2; Hydrogenase-3 component B [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNRFVIADPRLCIGCNTCMAACSQEHQAQGLQAQPRLTLVKTSHESAPQMCHHCEDAPCALVCPVNAITRQDGAIQLNES
LCVGCKLCGIACPFGAITLGGSKPLHIPANSNTPLAPPAPPAPLSVGPFLDWYPGIRSIAVKCDLCQFSDAGPACVRACP
THAIVLVDERRLEDASAAKRLNVLDAQRADLLALARLSGGNDAL

Sequences:

>Translated_204_residues
MNRFVIADPRLCIGCNTCMAACSQEHQAQGLQAQPRLTLVKTSHESAPQMCHHCEDAPCALVCPVNAITRQDGAIQLNES
LCVGCKLCGIACPFGAITLGGSKPLHIPANSNTPLAPPAPPAPLSVGPFLDWYPGIRSIAVKCDLCQFSDAGPACVRACP
THAIVLVDERRLEDASAAKRLNVLDAQRADLLALARLSGGNDAL
>Mature_204_residues
MNRFVIADPRLCIGCNTCMAACSQEHQAQGLQAQPRLTLVKTSHESAPQMCHHCEDAPCALVCPVNAITRQDGAIQLNES
LCVGCKLCGIACPFGAITLGGSKPLHIPANSNTPLAPPAPPAPLSVGPFLDWYPGIRSIAVKCDLCQFSDAGPACVRACP
THAIVLVDERRLEDASAAKRLNVLDAQRADLLALARLSGGNDAL

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789079, Length=201, Percent_Identity=64.1791044776119, Blast_Score=246, Evalue=6e-67,
Organism=Escherichia coli, GI87082114, Length=184, Percent_Identity=53.804347826087, Blast_Score=197, Evalue=3e-52,
Organism=Escherichia coli, GI1789067, Length=187, Percent_Identity=41.1764705882353, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1788811, Length=186, Percent_Identity=34.9462365591398, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI2367245, Length=180, Percent_Identity=37.2222222222222, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI87082180, Length=186, Percent_Identity=33.8709677419355, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI87082179, Length=176, Percent_Identity=38.6363636363636, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI2367345, Length=177, Percent_Identity=28.8135593220339, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=30.3867403314917, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 21458; Mature: 21458

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
9.3 %Cys+Met (Translated Protein)
7.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVIADPRLCIGCNTCMAACSQEHQAQGLQAQPRLTLVKTSHESAPQMCHHCEDAPCA
CCCEEEECCCEEEECHHHHHHHCHHHHHCCCCCCCCEEEEECCCCCHHHHHHCCCCCCEE
LVCPVNAITRQDGAIQLNESLCVGCKLCGIACPFGAITLGGSKPLHIPANSNTPLAPPAP
EEECCHHHHCCCCCEEECCCEEEHHEECCEECCCCEEEECCCCCEEEECCCCCCCCCCCC
PAPLSVGPFLDWYPGIRSIAVKCDLCQFSDAGPACVRACPTHAIVLVDERRLEDASAAKR
CCCCCCCCCCHHCCCCEEEEEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHH
LNVLDAQRADLLALARLSGGNDAL
HHHHCCCHHHEEEEEEECCCCCCC
>Mature Secondary Structure
MNRFVIADPRLCIGCNTCMAACSQEHQAQGLQAQPRLTLVKTSHESAPQMCHHCEDAPCA
CCCEEEECCCEEEECHHHHHHHCHHHHHCCCCCCCCEEEEECCCCCHHHHHHCCCCCCEE
LVCPVNAITRQDGAIQLNESLCVGCKLCGIACPFGAITLGGSKPLHIPANSNTPLAPPAP
EEECCHHHHCCCCCEEECCCEEEHHEECCEECCCCEEEECCCCCEEEECCCCCCCCCCCC
PAPLSVGPFLDWYPGIRSIAVKCDLCQFSDAGPACVRACPTHAIVLVDERRLEDASAAKR
CCCCCCCCCCHHCCCCEEEEEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHH
LNVLDAQRADLLALARLSGGNDAL
HHHHCCCHHHEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; H+; formate [C]

Specific reaction: 2 reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 formate = CO2 + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]