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The map label for this gene is rnhA

Identifier: 238921063

GI number: 238921063

Start: 3071544

End: 3072008

Strand: Direct

Name: rnhA

Synonym: NT01EI_3194

Alternate gene names: 238921063

Gene position: 3071544-3072008 (Clockwise)

Preceding gene: 238921061

Following gene: 238921065

Centisome position: 80.57

GC content: 60.0

Gene sequence:

>465_bases
ATGTTAAAGCAGGTTGAAATTTTCACCGATGGCTCCTGTCTGGGCAATCCGGGCCCGGGTGGCTATGGCGCCATTCTGCG
CTACCGTCAGCATGAAAAAGCGCTCAGTGCCGGCTATCGGCTGACCACTAACAACCGCATGGAGCTGATGGCGGCCATCG
TCGCCCTGGAGACGCTGACCAGCGCCTGCCAGGTCGCGCTGTTCAGCGATAGCCAGTATGTCCGTCAGGGCATTACCCAG
TGGATCCACGGCTGGAAACGACGCGACTGGAAAACGGCGGATAAAAAACCGGTGAAAAACGTCGATCTATGGCAGCGACT
GGATCAGGCCATCGGCCCACATCAGGTGGAGTGGATATGGATAAAAGGTCATGCTGGTCACCCAGAGAACGAGCGCTGCG
ATGAGCTGGCGCGCCGCGCCGCCGGGACACCGACCCAGGACGACAGCGGCTACACTCCCGGGTAG

Upstream 100 bases:

>100_bases
AAGGACGATTTGGCGGGCGATAGGGATACTCATTTTACTGTGCTCATCGTGTCACTTTATGTCAGACTCACGGTTTTGAT
CAGAGAGTAGTCTACCAGAG

Downstream 100 bases:

>100_bases
CCACGCCACCGGGGCCGCTAAACCGGCCGCCGTCTGATCTTTGCCGCGCTACCCAACGCGCGGCGGATACTATGATGCGC
CAGCAAGACGCGTTGCGGCG

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYRQHEKALSAGYRLTTNNRMELMAAIVALETLTSACQVALFSDSQYVRQGITQ
WIHGWKRRDWKTADKKPVKNVDLWQRLDQAIGPHQVEWIWIKGHAGHPENERCDELARRAAGTPTQDDSGYTPG

Sequences:

>Translated_154_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYRQHEKALSAGYRLTTNNRMELMAAIVALETLTSACQVALFSDSQYVRQGITQ
WIHGWKRRDWKTADKKPVKNVDLWQRLDQAIGPHQVEWIWIKGHAGHPENERCDELARRAAGTPTQDDSGYTPG
>Mature_154_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYRQHEKALSAGYRLTTNNRMELMAAIVALETLTSACQVALFSDSQYVRQGITQ
WIHGWKRRDWKTADKKPVKNVDLWQRLDQAIGPHQVEWIWIKGHAGHPENERCDELARRAAGTPTQDDSGYTPG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=142, Percent_Identity=33.8028169014084, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=80.1324503311258, Blast_Score=258, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=34.2857142857143, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45552509, Length=151, Percent_Identity=35.0993377483444, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137476, Length=151, Percent_Identity=35.0993377483444, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_EDWI9 (C5BEV5)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934578.1
- ProteinModelPortal:   C5BEV5
- GeneID:   7958711
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3194
- OMA:   NGWRTAD
- ProtClustDB:   CLSK2813576
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17320; Mature: 17320

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQVEIFTDGSCLGNPGPGGYGAILRYRQHEKALSAGYRLTTNNRMELMAAIVALETLT
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHH
SACQVALFSDSQYVRQGITQWIHGWKRRDWKTADKKPVKNVDLWQRLDQAIGPHQVEWIW
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEE
IKGHAGHPENERCDELARRAAGTPTQDDSGYTPG
EECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKQVEIFTDGSCLGNPGPGGYGAILRYRQHEKALSAGYRLTTNNRMELMAAIVALETLT
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHH
SACQVALFSDSQYVRQGITQWIHGWKRRDWKTADKKPVKNVDLWQRLDQAIGPHQVEWIW
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEE
IKGHAGHPENERCDELARRAAGTPTQDDSGYTPG
EECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA