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The map label for this gene is gloB

Identifier: 238921065

GI number: 238921065

Start: 3072780

End: 3073535

Strand: Direct

Name: gloB

Synonym: NT01EI_3196

Alternate gene names: 238921065

Gene position: 3072780-3073535 (Clockwise)

Preceding gene: 238921063

Following gene: 238921066

Centisome position: 80.6

GC content: 48.81

Gene sequence:

>756_bases
ATGGATCTTATCGGTCTTCCTACCCTACAGGATAACTACATCTGGCTGCTAATCAATCCGCAGCATCAATGTCTGATCGT
CGATCCCGGCGTCGCCGCGCCGGTTTTACACTATTTGACAGAGAATAGGATAACCCCTAAAGCCATCCTGCTGACGCACC
ATCATAATGACCACGTTGGCGGTGTTGCAGAGATCGTCCAGGCCTACCCTGAAATGCCGGTTTACGGTCCGGCAGAAACA
CAGGGACAGGGGTGTACCCAAGTCGTAGCCGATAACGATACGCTCTCCCTGCTAGGGTGCCAGATTTCTATCATGGCTCT
GCCGGGACATACGTTGGGCCATATGGCCTACTACAGCGCTCCGTACCTGTTTTGCGGCGATACGCTATTCTCCGCTGGCT
GTGGGCGCCTGTTTGAGGGCAGTGCGCAGCAAATGTTTGACTCGTTACAGCGCATTATACAACTTCCTGATAACACATTG
GTGTGCTGCGCTCACGAATATACTGAGTCAAATCTACGGTTTGCTCGGCATGTATTACCCAAAAACAGGGAAATAGAAAC
ATATCAGCAACACGTAACGATATTGCGGGCAAAACAGCAACCTACGGTGCCTTCAACGCTACGGATTGAGATGGAAATAA
ATCCATTCTTACGCTGTTATGATTATGATTTGCAAAGGAATGTGGGTTTTCCTACGCAACCTAATGAGATTTGGCGAGTT
TTTGCCTGTCTACGCAACATGAAAGACAGTTTTTAA

Upstream 100 bases:

>100_bases
GAGTCCCCACGGGATTTGCCGCCAGGTAAAAGGCGCGTTTAGCGTGCGCATGCTCAGTGCCGGTCTCATCGTCACCTCTG
ACTTTGGAGTAGATAACATC

Downstream 100 bases:

>100_bases
AGCTTTTTCTTGTGTTGTGCAGTCAAGCAACGTATTATTGCTCGTCTTTTAATCAACAGCGACGCACACATGAAGGCTAA
AGCGATAATCTTCGCCTCCG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MDLIGLPTLQDNYIWLLINPQHQCLIVDPGVAAPVLHYLTENRITPKAILLTHHHNDHVGGVAEIVQAYPEMPVYGPAET
QGQGCTQVVADNDTLSLLGCQISIMALPGHTLGHMAYYSAPYLFCGDTLFSAGCGRLFEGSAQQMFDSLQRIIQLPDNTL
VCCAHEYTESNLRFARHVLPKNREIETYQQHVTILRAKQQPTVPSTLRIEMEINPFLRCYDYDLQRNVGFPTQPNEIWRV
FACLRNMKDSF

Sequences:

>Translated_251_residues
MDLIGLPTLQDNYIWLLINPQHQCLIVDPGVAAPVLHYLTENRITPKAILLTHHHNDHVGGVAEIVQAYPEMPVYGPAET
QGQGCTQVVADNDTLSLLGCQISIMALPGHTLGHMAYYSAPYLFCGDTLFSAGCGRLFEGSAQQMFDSLQRIIQLPDNTL
VCCAHEYTESNLRFARHVLPKNREIETYQQHVTILRAKQQPTVPSTLRIEMEINPFLRCYDYDLQRNVGFPTQPNEIWRV
FACLRNMKDSF
>Mature_251_residues
MDLIGLPTLQDNYIWLLINPQHQCLIVDPGVAAPVLHYLTENRITPKAILLTHHHNDHVGGVAEIVQAYPEMPVYGPAET
QGQGCTQVVADNDTLSLLGCQISIMALPGHTLGHMAYYSAPYLFCGDTLFSAGCGRLFEGSAQQMFDSLQRIIQLPDNTL
VCCAHEYTESNLRFARHVLPKNREIETYQQHVTILRAKQQPTVPSTLRIEMEINPFLRCYDYDLQRNVGFPTQPNEIWRV
FACLRNMKDSF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=36.1538461538462, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI94538322, Length=255, Percent_Identity=36.4705882352941, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI116642887, Length=262, Percent_Identity=37.7862595419847, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI21703352, Length=262, Percent_Identity=37.7862595419847, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI14150041, Length=237, Percent_Identity=36.2869198312236, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI46361987, Length=195, Percent_Identity=36.9230769230769, Blast_Score=122, Evalue=2e-28,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=54.1832669322709, Blast_Score=267, Evalue=4e-73,
Organism=Escherichia coli, GI1787158, Length=166, Percent_Identity=30.7228915662651, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=254, Percent_Identity=32.2834645669291, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6320478, Length=261, Percent_Identity=32.183908045977, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324614, Length=266, Percent_Identity=27.8195488721804, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=36.7816091954023, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=36.7816091954023, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=36.7816091954023, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_EDWI9 (C5BEP2)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934580.1
- ProteinModelPortal:   C5BEP2
- GeneID:   7958713
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3196
- OMA:   WCAHEYT
- ProtClustDB:   CLSK2813577
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28370; Mature: 28370

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLIGLPTLQDNYIWLLINPQHQCLIVDPGVAAPVLHYLTENRITPKAILLTHHHNDHVG
CCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
GVAEIVQAYPEMPVYGPAETQGQGCTQVVADNDTLSLLGCQISIMALPGHTLGHMAYYSA
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEEECCCCHHHHHHHHCC
PYLFCGDTLFSAGCGRLFEGSAQQMFDSLQRIIQLPDNTLVCCAHEYTESNLRFARHVLP
CEEEECHHHHHHCCCHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCHHHHHHHHHHCC
KNREIETYQQHVTILRAKQQPTVPSTLRIEMEINPFLRCYDYDLQRNVGFPTQPNEIWRV
CCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEECCHHCCCCCCCCHHHHHHH
FACLRNMKDSF
HHHHHHHHCCC
>Mature Secondary Structure
MDLIGLPTLQDNYIWLLINPQHQCLIVDPGVAAPVLHYLTENRITPKAILLTHHHNDHVG
CCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
GVAEIVQAYPEMPVYGPAETQGQGCTQVVADNDTLSLLGCQISIMALPGHTLGHMAYYSA
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEEECCCCHHHHHHHHCC
PYLFCGDTLFSAGCGRLFEGSAQQMFDSLQRIIQLPDNTLVCCAHEYTESNLRFARHVLP
CEEEECHHHHHHCCCHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCHHHHHHHHHHCC
KNREIETYQQHVTILRAKQQPTVPSTLRIEMEINPFLRCYDYDLQRNVGFPTQPNEIWRV
CCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEECCHHCCCCCCCCHHHHHHH
FACLRNMKDSF
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA