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The map label for this gene is ushA [H]

Identifier: 238893449

GI number: 238893449

Start: 1292693

End: 1294345

Strand: Direct

Name: ushA [H]

Synonym: KP1_1339

Alternate gene names: 238893449

Gene position: 1292693-1294345 (Clockwise)

Preceding gene: 238893446

Following gene: 238893453

Centisome position: 24.63

GC content: 56.44

Gene sequence:

>1653_bases
ATGCATTATTTCAAACACAGCGTAGCGCTGGCGCTATTCGCCGCTTTATCGCTGGGCAGCTTGTCGGCACAGGCCTATGA
GCAGGATAAAACCTATAAAATTACCATCCTGCACACCAACGATCACCACGGCCATTTCTGGCGCAATGACTACGGCGAAT
ATGGCCTGGCGGCGCAGAAAACGCTGGTCGATGGCATCCGGAAAGAGGTGGCGGCGGAAGGGGGAAGCGTCCTGCTGTTA
TCCGGCGGCGATATTAATACCGGCGTACCGGAGTCTGATTTACAGGATGCAGAGCCCGATTTTCGCGGCATGAACCTGAT
CGGCTACGACGCGATGGCGGTGGGAAACCATGAATTCGATAATCCGCTGAGCGTACTGCGCCAGCAGGAAAAGTGGGCTA
AGTTCCCGTTCCTGTCGGCCAATATTTATCAGAAAAGCACCGGCGAACGGCTGTTCAAACCCTGGGCGTTGTTTAAGCGC
GGTGGGCTGAAAATCGCGGTGATTGGCTTAACCACCGACGATACGGCCAAGATTGGCAATCCGGAATATTTCACCGATAT
TGAATTCCGCAAACCGGCGGAAGAGGCGAAGCTGGTTATCCAGGAGCTGCAGCAGAATGAAAAGCCGGACGTGATCCTGG
CCACCACCCATATGGGCCATTACGACAATGGCAACCACGGCTCTAATGCGCCGGGCGATGTTGAAATGGCGCGGAGTTTA
CCTGCGGGATCGCTGGCGATGATCGTCGGCGGTCACTCGCAGGATCCGGTATGCATGGCGGCGGAAAATAAAAAGCAGGT
CGATTATGTGCCAGGCACCCCGTGCGCTCCGGATCGTCAGAACGGCATCTGGATCGTCCAGGCGCATGAGTGGGGCAAAT
ACGTCGGCCGCGCCGATTTTGAGTTCCGCAACGGCGAGATGAAGCTGGTGCACTATCAGCTGATCCCGGTCAACCTGAAG
AAAAAAGTGACTTACGATAACGGCCAAAGCGAGCGGGTGCTGTATACGCCGCAGATCGCCGAGAACCCGCAGATGATGTC
GCTGCTGACGCCGTTCCAGAACAAAGGCAAGGCGCAGCTGCAGGTCAAAATCGGCAGCGTGAATGGTCATCTGGAGGGCG
ACCGCAGTAAAGTGCGCTTTGTGCAGACCAATATGGGCCACCTGCTGCTGGCGGCGCAAATGGCGCGCAGCAACGCGGAT
TTCGCGGTGATGAGCGGCGGCGGGATCCGCGACTCCATTGAGGCGGGGGATATCACCTACAAAGATGTGATGAAGGTCCA
GCCGTTCGGCAATGTGCTGACCTATGTCGATATGAACGGCAAGGAGGTAGTGGATTACCTGACCGCCGTCGCGCAGATGA
AGCCGGATTCCGGCGCTTATCCGCAGTTTGCCAACGTCAGTTTCGTGGCCAAAGACGGTAAGCTGAACGATCTGAAAATC
AAAGGCGAACCGGTGGATCCGGCGAAGACCTATCGGATGGCGACGCTGAGCTTTAACGCCACCGGCGGCGATGGCTATCC
GAACATCGCCGATAAACCCGGGTATGTGAATACCGGCTTTATCGATGCCGAAGTGCTGAAAGAGTATATCGAGAAGAACT
CGCCGCTGGATGCCGCCGCCTATGAGCCGAAAGGCGAGGTGAGCTGGCAGTAA

Upstream 100 bases:

>100_bases
TTGATAAGGTTTGAAGGAAGTCGCAATTCTGAAAACATATTTGTCAACGCGACTAACTCGCTATACTTTCGGCACTTGGG
ATTAAGTCAGGGAGTGAAGC

Downstream 100 bases:

>100_bases
GCCTGGGCTTCAGGTTGGCTATTTCCCCGGTGGCGCTACGCTTACCGGGGCTACAAGTTGGGTATCCCGGATAAGGCGCG
TGCGCCGCTATCCGGGATCG

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MHYFKHSVALALFAALSLGSLSAQAYEQDKTYKITILHTNDHHGHFWRNDYGEYGLAAQKTLVDGIRKEVAAEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLSVLRQQEKWAKFPFLSANIYQKSTGERLFKPWALFKR
GGLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEAKLVIQELQQNEKPDVILATTHMGHYDNGNHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCAPDRQNGIWIVQAHEWGKYVGRADFEFRNGEMKLVHYQLIPVNLK
KKVTYDNGQSERVLYTPQIAENPQMMSLLTPFQNKGKAQLQVKIGSVNGHLEGDRSKVRFVQTNMGHLLLAAQMARSNAD
FAVMSGGGIRDSIEAGDITYKDVMKVQPFGNVLTYVDMNGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKDGKLNDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPNIADKPGYVNTGFIDAEVLKEYIEKNSPLDAAAYEPKGEVSWQ

Sequences:

>Translated_550_residues
MHYFKHSVALALFAALSLGSLSAQAYEQDKTYKITILHTNDHHGHFWRNDYGEYGLAAQKTLVDGIRKEVAAEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLSVLRQQEKWAKFPFLSANIYQKSTGERLFKPWALFKR
GGLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEAKLVIQELQQNEKPDVILATTHMGHYDNGNHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCAPDRQNGIWIVQAHEWGKYVGRADFEFRNGEMKLVHYQLIPVNLK
KKVTYDNGQSERVLYTPQIAENPQMMSLLTPFQNKGKAQLQVKIGSVNGHLEGDRSKVRFVQTNMGHLLLAAQMARSNAD
FAVMSGGGIRDSIEAGDITYKDVMKVQPFGNVLTYVDMNGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKDGKLNDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPNIADKPGYVNTGFIDAEVLKEYIEKNSPLDAAAYEPKGEVSWQ
>Mature_550_residues
MHYFKHSVALALFAALSLGSLSAQAYEQDKTYKITILHTNDHHGHFWRNDYGEYGLAAQKTLVDGIRKEVAAEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLSVLRQQEKWAKFPFLSANIYQKSTGERLFKPWALFKR
GGLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEAKLVIQELQQNEKPDVILATTHMGHYDNGNHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCAPDRQNGIWIVQAHEWGKYVGRADFEFRNGEMKLVHYQLIPVNLK
KKVTYDNGQSERVLYTPQIAENPQMMSLLTPFQNKGKAQLQVKIGSVNGHLEGDRSKVRFVQTNMGHLLLAAQMARSNAD
FAVMSGGGIRDSIEAGDITYKDVMKVQPFGNVLTYVDMNGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKDGKLNDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPNIADKPGYVNTGFIDAEVLKEYIEKNSPLDAAAYEPKGEVSWQ

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm. Note=Exported from the cell, except a small proportion that is internally localized [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=578, Percent_Identity=24.5674740484429, Blast_Score=119, Evalue=9e-27,
Organism=Escherichia coli, GI1786687, Length=550, Percent_Identity=87.0909090909091, Blast_Score=1018, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=576, Percent_Identity=22.2222222222222, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922446, Length=480, Percent_Identity=25.8333333333333, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24654424, Length=480, Percent_Identity=25.8333333333333, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI28573524, Length=554, Percent_Identity=23.2851985559567, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922444, Length=541, Percent_Identity=22.550831792976, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI221329836, Length=566, Percent_Identity=22.2614840989399, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24641187, Length=564, Percent_Identity=21.2765957446809, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 60564; Mature: 60564

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYFKHSVALALFAALSLGSLSAQAYEQDKTYKITILHTNDHHGHFWRNDYGEYGLAAQK
CCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHH
TLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFD
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLSVLRQQEKWAKFPFLSANIYQKSTGERLFKPWALFKRGGLKIAVIGLTTDDTAKIGN
CHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PEYFTDIEFRKPAEEAKLVIQELQQNEKPDVILATTHMGHYDNGNHGSNAPGDVEMARSL
CCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
PAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCAPDRQNGIWIVQAHEWGKYVGRADF
CCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
EFRNGEMKLVHYQLIPVNLKKKVTYDNGQSERVLYTPQIAENPQMMSLLTPFQNKGKAQL
EEECCCEEEEEEEEEEEEEEEEEEECCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCEEE
QVKIGSVNGHLEGDRSKVRFVQTNMGHLLLAAQMARSNADFAVMSGGGIRDSIEAGDITY
EEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCH
KDVMKVQPFGNVLTYVDMNGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKDGKLNDLKI
HHHEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEE
KGEPVDPAKTYRMATLSFNATGGDGYPNIADKPGYVNTGFIDAEVLKEYIEKNSPLDAAA
ECCCCCHHHHEEEEEEEEECCCCCCCCCCCCCCCEEECCEECHHHHHHHHHCCCCCCCCC
YEPKGEVSWQ
CCCCCCCCCC
>Mature Secondary Structure
MHYFKHSVALALFAALSLGSLSAQAYEQDKTYKITILHTNDHHGHFWRNDYGEYGLAAQK
CCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHH
TLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFD
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLSVLRQQEKWAKFPFLSANIYQKSTGERLFKPWALFKRGGLKIAVIGLTTDDTAKIGN
CHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PEYFTDIEFRKPAEEAKLVIQELQQNEKPDVILATTHMGHYDNGNHGSNAPGDVEMARSL
CCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
PAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCAPDRQNGIWIVQAHEWGKYVGRADF
CCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
EFRNGEMKLVHYQLIPVNLKKKVTYDNGQSERVLYTPQIAENPQMMSLLTPFQNKGKAQL
EEECCCEEEEEEEEEEEEEEEEEEECCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCEEE
QVKIGSVNGHLEGDRSKVRFVQTNMGHLLLAAQMARSNADFAVMSGGGIRDSIEAGDITY
EEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCH
KDVMKVQPFGNVLTYVDMNGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKDGKLNDLKI
HHHEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEE
KGEPVDPAKTYRMATLSFNATGGDGYPNIADKPGYVNTGFIDAEVLKEYIEKNSPLDAAA
ECCCCCHHHHEEEEEEEEECCCCCCCCCCCCCCCEEECCEECHHHHHHHHHCCCCCCCCC
YEPKGEVSWQ
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3012467; 9278503; 9298646; 10331872; 11491293; 11491294 [H]