Click here to switch to the map view.

The map label for this gene is pulL [H]

Identifier: 238893131

GI number: 238893131

Start: 961728

End: 962921

Strand: Reverse

Name: pulL [H]

Synonym: KP1_0980

Alternate gene names: 238893131

Gene position: 962921-961728 (Counterclockwise)

Preceding gene: 238893132

Following gene: 238893130

Centisome position: 18.35

GC content: 67.5

Gene sequence:

>1194_bases
ATGAACAAGATTAATGCTTCTCCCCAGGCCATGCTGATCGTGCGCCTCGCCGCCGCGCAGGCGCCGCTGCACTGGCAACT
CTTCGCCCCCGGCGAGCCGCACCACGAGGCCAGCGGCCGGTGGCCGACTGACGACGCCAGCCCTTTCCCGGCGCTGGCTG
AGCAGTACCCGGCCTGGGTGCTGATCCCGGCCAGCGACTGCGCGTTTCACTCGCTCACCCTACCGGCGGGCCTGCGTAAA
CCGCCGCTGCAGGTGGCCCCGTTTCTGCTGGAAGAGCAGCTGGCAGACGATGTGGAAGCCACCCATTTTGCCCTGCTCCA
CCGCCAGCAGGCCCAGTGCGAGATCGTCGCCGTACAGCGGCAAAAAATGCGCGACTGGCTGGCGCGCTGCGAATCGCTCT
CCTTACAGCCCCTGGCGTTGACGCCCGACGTGCTGGCCCTGCCCTGGCAACCGCCGGCGTGGAGCGCGGTGCAGGTGGAC
GAGCAGTGGCTGATCCGCCACCAGCCGTGGGGCGGCATGGCAGCGGAAAACGTCTGGCTGACGGAGCTGCTGCAGAGCGA
GGCGGAAGAGCACGTCATCGACAGCTATTCGCCGCCGCCCGCGGCGCCGGGCGTCTGGCGGGAGCAGCCTGCGCAGACAT
TGCTGACCCTTGCCGCACGCCATCCGGCAGCGCAGAAACTCAGTCTGCTGCAGGGGGAGTTCGCCGTCCGGTGGCGGTCT
GCGCAGGCGAGCTGGCGTCCGGCGCGGTATGCCGCGCTAGCCCTGGCGTTACTGGCAGGGGCAAACAGCGTGCTGGACCA
TCGCGATCTGGCGCGTCAGGCCGAGGCGGCGCAACAGGCCAGCCGGGCCTTCTATCACCGCTGGTTTCCGACGGAGAAAA
AGGTGATTAACCCGCGACTGCAGATGCAGCAGCACCTGCAAACCCTCACCCGTCAGGCGCAGCATGCCCCCCTCGTCGAC
CGCCTCAGCGCCTTGCAAAACATCCTCAGCGAAACGCCGGGGATCCGTCTGCGAGCGCTGAGCTGGGATGCCGCGGGCAA
TCGTCTGCAGCTGGATATCGCCGCGGTCTCTTCCCGGGCGCTGGAGCAGTTTACCCAGCGGGCGCAGCCGCGGTTTCGCG
TCCGGCCGGGCGACATGATCACGAAACCGGATGGCATTGAGGGACAACTGACGCTGGAGGAAAACGATGGCTAA

Upstream 100 bases:

>100_bases
GTGACCGGCAATCTCCGCTTTCAGCTGCACAGCGTCCTGCAACAATCGGGGCGCACGTTTACCGTCGTCCAGCGGCGATA
TGGACTCTCTATGGTGGTCG

Downstream 100 bases:

>100_bases
TCTGCTTATCTGGTGGCGGCAGCGCACGCCGTCGGAGCAGCGCCTGTTGCTTGGTCTGACAGGGCTGCTGGCGGCATGCG
CCTTCTGGTATGGGCTGTGG

Product: pullulanase-specific type II secretion system integral cytoplasmic membrane protein

Products: NA

Alternate protein names: Pullulanase secretion protein pulL [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKINASPQAMLIVRLAAAQAPLHWQLFAPGEPHHEASGRWPTDDASPFPALAEQYPAWVLIPASDCAFHSLTLPAGLRK
PPLQVAPFLLEEQLADDVEATHFALLHRQQAQCEIVAVQRQKMRDWLARCESLSLQPLALTPDVLALPWQPPAWSAVQVD
EQWLIRHQPWGGMAAENVWLTELLQSEAEEHVIDSYSPPPAAPGVWREQPAQTLLTLAARHPAAQKLSLLQGEFAVRWRS
AQASWRPARYAALALALLAGANSVLDHRDLARQAEAAQQASRAFYHRWFPTEKKVINPRLQMQQHLQTLTRQAQHAPLVD
RLSALQNILSETPGIRLRALSWDAAGNRLQLDIAAVSSRALEQFTQRAQPRFRVRPGDMITKPDGIEGQLTLEENDG

Sequences:

>Translated_397_residues
MNKINASPQAMLIVRLAAAQAPLHWQLFAPGEPHHEASGRWPTDDASPFPALAEQYPAWVLIPASDCAFHSLTLPAGLRK
PPLQVAPFLLEEQLADDVEATHFALLHRQQAQCEIVAVQRQKMRDWLARCESLSLQPLALTPDVLALPWQPPAWSAVQVD
EQWLIRHQPWGGMAAENVWLTELLQSEAEEHVIDSYSPPPAAPGVWREQPAQTLLTLAARHPAAQKLSLLQGEFAVRWRS
AQASWRPARYAALALALLAGANSVLDHRDLARQAEAAQQASRAFYHRWFPTEKKVINPRLQMQQHLQTLTRQAQHAPLVD
RLSALQNILSETPGIRLRALSWDAAGNRLQLDIAAVSSRALEQFTQRAQPRFRVRPGDMITKPDGIEGQLTLEENDG
>Mature_397_residues
MNKINASPQAMLIVRLAAAQAPLHWQLFAPGEPHHEASGRWPTDDASPFPALAEQYPAWVLIPASDCAFHSLTLPAGLRK
PPLQVAPFLLEEQLADDVEATHFALLHRQQAQCEIVAVQRQKMRDWLARCESLSLQPLALTPDVLALPWQPPAWSAVQVD
EQWLIRHQPWGGMAAENVWLTELLQSEAEEHVIDSYSPPPAAPGVWREQPAQTLLTLAARHPAAQKLSLLQGEFAVRWRS
AQASWRPARYAALALALLAGANSVLDHRDLARQAEAAQQASRAFYHRWFPTEKKVINPRLQMQQHLQTLTRQAQHAPLVD
RLSALQNILSETPGIRLRALSWDAAGNRLQLDIAAVSSRALEQFTQRAQPRFRVRPGDMITKPDGIEGQLTLEENDG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG3297

COG function: function code U; Type II secretory pathway, component PulL

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP L family [H]

Homologues:

Organism=Escherichia coli, GI87082244, Length=372, Percent_Identity=26.3440860215054, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007812 [H]

Pfam domain/function: PF05134 GspL [H]

EC number: NA

Molecular weight: Translated: 44317; Mature: 44317

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKINASPQAMLIVRLAAAQAPLHWQLFAPGEPHHEASGRWPTDDASPFPALAEQYPAWV
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEE
LIPASDCAFHSLTLPAGLRKPPLQVAPFLLEEQLADDVEATHFALLHRQQAQCEIVAVQR
EEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QKMRDWLARCESLSLQPLALTPDVLALPWQPPAWSAVQVDEQWLIRHQPWGGMAAENVWL
HHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCCEEEECHHHHHEECCCCCCCCCHHHH
TELLQSEAEEHVIDSYSPPPAAPGVWREQPAQTLLTLAARHPAAQKLSLLQGEFAVRWRS
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHEEEEC
AQASWRPARYAALALALLAGANSVLDHRDLARQAEAAQQASRAFYHRWFPTEKKVINPRL
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHH
QMQQHLQTLTRQAQHAPLVDRLSALQNILSETPGIRLRALSWDAAGNRLQLDIAAVSSRA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEHHHHHHHH
LEQFTQRAQPRFRVRPGDMITKPDGIEGQLTLEENDG
HHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MNKINASPQAMLIVRLAAAQAPLHWQLFAPGEPHHEASGRWPTDDASPFPALAEQYPAWV
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEE
LIPASDCAFHSLTLPAGLRKPPLQVAPFLLEEQLADDVEATHFALLHRQQAQCEIVAVQR
EEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QKMRDWLARCESLSLQPLALTPDVLALPWQPPAWSAVQVDEQWLIRHQPWGGMAAENVWL
HHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCCEEEECHHHHHEECCCCCCCCCHHHH
TELLQSEAEEHVIDSYSPPPAAPGVWREQPAQTLLTLAARHPAAQKLSLLQGEFAVRWRS
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHEEEEC
AQASWRPARYAALALALLAGANSVLDHRDLARQAEAAQQASRAFYHRWFPTEKKVINPRL
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHH
QMQQHLQTLTRQAQHAPLVDRLSALQNILSETPGIRLRALSWDAAGNRLQLDIAAVSSRA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEHHHHHHHH
LEQFTQRAQPRFRVRPGDMITKPDGIEGQLTLEENDG
HHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162463 [H]