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The map label for this gene is pulK [H]

Identifier: 238893132

GI number: 238893132

Start: 962908

End: 963861

Strand: Reverse

Name: pulK [H]

Synonym: KP1_0982

Alternate gene names: 238893132

Gene position: 963861-962908 (Counterclockwise)

Preceding gene: 238893133

Following gene: 238893131

Centisome position: 18.36

GC content: 65.41

Gene sequence:

>954_bases
ATGGTGCTTTTCATCCTCGCCCTGATGATGATCCTCGCCAGCGCCATGACCGAGCGCACCGCGGTCATGTATCAGCATAC
CGCCGTGACCCTCGATAATTTGCAGGCCAGATGGTACGCCCTGGCGGCGGAAAACATGGCCGCCGCGCTGCTGCAACGCG
ATGCGCTGGACTCGCCCAGTCAAACGAACCTGGCGCAAACCTGGGCGCAGGAGGGGCGCCGCTTTACCCTCGACGATGGC
GAAATCCGCGCCACGATCCGCGACGGGCACGCCTGCTTCAACCTCAATGCCATCAACCATCGCGCGGATGAGGCTGGCGA
CGGAACGCCCTACCCGACCGACGTCTTCGTTCGGCTGCTGGCGCTGCTCGGCGAGCCGCCGCTGCGCGCCAGCCAGATCG
CCGCCGCCCTTGGCGACTGGACGGACAGCGACGGCCAGCCGCGGCTGAACGGAGCGGAGGACGAGGTCTATATGGCGCAA
ACCCCGGGCTATCTCGCCGCCAACCAGCCGATGCAGGATGTCAGCGAGTTGCGCCTGCTGGCCGGGATGGACGCCGCGCT
CTATCAGCGCCTGCTCCCCTTCGTCTGCGTCCAGCCGGATGACGCGCTGCAGGTCAATGTCAACACCCTGCGCCCCTCCC
AGGCCGCCCTGCTGGTGGCCCTCTTTCCCGGCGATCTGACGCTGCAGGAGGCGCAACAGCTGCTGCACAACCGCCCGCGA
ACCGGGTGGAGCAGCGTCGCCGCCTTTCTCGCGCAGCCGACGCTGCAGAAAACCGATACCACGCTTGCCCGTCCCTGGCT
GACGGTGCACAGCGCACGCTTTATCGCCGCTTTTAGCGTGGTGACCGGCAATCTCCGCTTTCAGCTGCACAGCGTCCTGC
AACAATCGGGGCGCACGTTTACCGTCGTCCAGCGGCGATATGGACTCTCTATGGTGGTCGATGAACAAGATTAA

Upstream 100 bases:

>100_bases
TCACCCTGACGCTGGCGAACAGCGGGGAGATAACCCGCCTGTTTTTACTCACCCCGGGAGGCGGCCAGTGATAAATCGGC
AACGCGGCGTCGCGCTCCTG

Downstream 100 bases:

>100_bases
TGCTTCTCCCCAGGCCATGCTGATCGTGCGCCTCGCCGCCGCGCAGGCGCCGCTGCACTGGCAACTCTTCGCCCCCGGCG
AGCCGCACCACGAGGCCAGC

Product: pullulanase-specific type II secretion system component K

Products: NA

Alternate protein names: Pullulanase secretion protein pulK [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MVLFILALMMILASAMTERTAVMYQHTAVTLDNLQARWYALAAENMAAALLQRDALDSPSQTNLAQTWAQEGRRFTLDDG
EIRATIRDGHACFNLNAINHRADEAGDGTPYPTDVFVRLLALLGEPPLRASQIAAALGDWTDSDGQPRLNGAEDEVYMAQ
TPGYLAANQPMQDVSELRLLAGMDAALYQRLLPFVCVQPDDALQVNVNTLRPSQAALLVALFPGDLTLQEAQQLLHNRPR
TGWSSVAAFLAQPTLQKTDTTLARPWLTVHSARFIAAFSVVTGNLRFQLHSVLQQSGRTFTVVQRRYGLSMVVDEQD

Sequences:

>Translated_317_residues
MVLFILALMMILASAMTERTAVMYQHTAVTLDNLQARWYALAAENMAAALLQRDALDSPSQTNLAQTWAQEGRRFTLDDG
EIRATIRDGHACFNLNAINHRADEAGDGTPYPTDVFVRLLALLGEPPLRASQIAAALGDWTDSDGQPRLNGAEDEVYMAQ
TPGYLAANQPMQDVSELRLLAGMDAALYQRLLPFVCVQPDDALQVNVNTLRPSQAALLVALFPGDLTLQEAQQLLHNRPR
TGWSSVAAFLAQPTLQKTDTTLARPWLTVHSARFIAAFSVVTGNLRFQLHSVLQQSGRTFTVVQRRYGLSMVVDEQD
>Mature_317_residues
MVLFILALMMILASAMTERTAVMYQHTAVTLDNLQARWYALAAENMAAALLQRDALDSPSQTNLAQTWAQEGRRFTLDDG
EIRATIRDGHACFNLNAINHRADEAGDGTPYPTDVFVRLLALLGEPPLRASQIAAALGDWTDSDGQPRLNGAEDEVYMAQ
TPGYLAANQPMQDVSELRLLAGMDAALYQRLLPFVCVQPDDALQVNVNTLRPSQAALLVALFPGDLTLQEAQQLLHNRPR
TGWSSVAAFLAQPTLQKTDTTLARPWLTVHSARFIAAFSVVTGNLRFQLHSVLQQSGRTFTVVQRRYGLSMVVDEQD

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=227, Percent_Identity=37.8854625550661, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 34920; Mature: 34920

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLFILALMMILASAMTERTAVMYQHTAVTLDNLQARWYALAAENMAAALLQRDALDSPS
CHHHHHHHHHHHHHHHHHHHHHEEHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QTNLAQTWAQEGRRFTLDDGEIRATIRDGHACFNLNAINHRADEAGDGTPYPTDVFVRLL
CHHHHHHHHHCCCEEEECCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
ALLGEPPLRASQIAAALGDWTDSDGQPRLNGAEDEVYMAQTPGYLAANQPMQDVSELRLL
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEECCCCHHHHHHHHHH
AGMDAALYQRLLPFVCVQPDDALQVNVNTLRPSQAALLVALFPGDLTLQEAQQLLHNRPR
HCCCHHHHHHHHHHEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
TGWSSVAAFLAQPTLQKTDTTLARPWLTVHSARFIAAFSVVTGNLRFQLHSVLQQSGRTF
CCHHHHHHHHHCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEE
TVVQRRYGLSMVVDEQD
EEEEHHCCCEEEEECCC
>Mature Secondary Structure
MVLFILALMMILASAMTERTAVMYQHTAVTLDNLQARWYALAAENMAAALLQRDALDSPS
CHHHHHHHHHHHHHHHHHHHHHEEHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QTNLAQTWAQEGRRFTLDDGEIRATIRDGHACFNLNAINHRADEAGDGTPYPTDVFVRLL
CHHHHHHHHHCCCEEEECCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
ALLGEPPLRASQIAAALGDWTDSDGQPRLNGAEDEVYMAQTPGYLAANQPMQDVSELRLL
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEECCCCHHHHHHHHHH
AGMDAALYQRLLPFVCVQPDDALQVNVNTLRPSQAALLVALFPGDLTLQEAQQLLHNRPR
HCCCHHHHHHHHHHEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
TGWSSVAAFLAQPTLQKTDTTLARPWLTVHSARFIAAFSVVTGNLRFQLHSVLQQSGRTF
CCHHHHHHHHHCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEE
TVVQRRYGLSMVVDEQD
EEEEHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2129543; 2162463 [H]