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The map label for this gene is pulM [H]

Identifier: 238893130

GI number: 238893130

Start: 961250

End: 961735

Strand: Reverse

Name: pulM [H]

Synonym: KP1_0979

Alternate gene names: 238893130

Gene position: 961735-961250 (Counterclockwise)

Preceding gene: 238893131

Following gene: 238893129

Centisome position: 18.32

GC content: 68.11

Gene sequence:

>486_bases
ATGGCTAATCTGCTTATCTGGTGGCGGCAGCGCACGCCGTCGGAGCAGCGCCTGTTGCTTGGTCTGACAGGGCTGCTGGC
GGCATGCGCCTTCTGGTATGGGCTGTGGCAGCCCTGGCGAGCCCGCGAGGCGCAGTGGCGGCAAACCCTGGTGAAAGAGC
AGGCCAGCCTGCGCTGGATGACGGAGCAGGCTCCGCGCCTGCAGCAGCTCAGCCAGCAGCCCGCGCCGGCGGCAAAAGAG
GCGCTCACCGCGCTGGTGATGCGCGAGGCGGCGAGCCACGGACTGACGGTCGTCCGCCTGCAGCCGCAGGGAAAACGTCT
GCAGATCACCCTTCAGCCCTGCGCTTTCCAGGTGCTGATCGACTGGCTGGACGCTCCGGCGATGCGCGGCGTCAACGCGA
TCTCGCTGTCCGTGACCGGGCAGCCGTCGCGACCCGGCTGGGTCACGGTCAACCATCTGCTGCTGGAGCGCGACGATGAA
GGCTAA

Upstream 100 bases:

>100_bases
CAGTTTACCCAGCGGGCGCAGCCGCGGTTTCGCGTCCGGCCGGGCGACATGATCACGAAACCGGATGGCATTGAGGGACA
ACTGACGCTGGAGGAAAACG

Downstream 100 bases:

>100_bases
CTGGATCGCGGGCGGACTGCTGCTGGTCCTCTATCTGTTGTGGCTCACCGCCACCGCTCCGGCGCGCCTGCTGGCTCCCA
TGCTGCCGCCCGGCGTACAG

Product: pullulanase-specific type II secretion system component M

Products: NA

Alternate protein names: Pullulanase secretion protein pulM [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MANLLIWWRQRTPSEQRLLLGLTGLLAACAFWYGLWQPWRAREAQWRQTLVKEQASLRWMTEQAPRLQQLSQQPAPAAKE
ALTALVMREAASHGLTVVRLQPQGKRLQITLQPCAFQVLIDWLDAPAMRGVNAISLSVTGQPSRPGWVTVNHLLLERDDE
G

Sequences:

>Translated_161_residues
MANLLIWWRQRTPSEQRLLLGLTGLLAACAFWYGLWQPWRAREAQWRQTLVKEQASLRWMTEQAPRLQQLSQQPAPAAKE
ALTALVMREAASHGLTVVRLQPQGKRLQITLQPCAFQVLIDWLDAPAMRGVNAISLSVTGQPSRPGWVTVNHLLLERDDE
G
>Mature_160_residues
ANLLIWWRQRTPSEQRLLLGLTGLLAACAFWYGLWQPWRAREAQWRQTLVKEQASLRWMTEQAPRLQQLSQQPAPAAKEA
LTALVMREAASHGLTVVRLQPQGKRLQITLQPCAFQVLIDWLDAPAMRGVNAISLSVTGQPSRPGWVTVNHLLLERDDEG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG3149

COG function: function code U; Type II secretory pathway, component PulM

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP M family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007690
- InterPro:   IPR023229 [H]

Pfam domain/function: PF04612 GspM [H]

EC number: NA

Molecular weight: Translated: 18270; Mature: 18139

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLLIWWRQRTPSEQRLLLGLTGLLAACAFWYGLWQPWRAREAQWRQTLVKEQASLRWM
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TEQAPRLQQLSQQPAPAAKEALTALVMREAASHGLTVVRLQPQGKRLQITLQPCAFQVLI
HHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECHHHHHHHH
DWLDAPAMRGVNAISLSVTGQPSRPGWVTVNHLLLERDDEG
HHHCCHHHCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCC
>Mature Secondary Structure 
ANLLIWWRQRTPSEQRLLLGLTGLLAACAFWYGLWQPWRAREAQWRQTLVKEQASLRWM
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TEQAPRLQQLSQQPAPAAKEALTALVMREAASHGLTVVRLQPQGKRLQITLQPCAFQVLI
HHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECHHHHHHHH
DWLDAPAMRGVNAISLSVTGQPSRPGWVTVNHLLLERDDEG
HHHCCHHHCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2162463 [H]