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The map label for this gene is iolD [H]

Identifier: 238892758

GI number: 238892758

Start: 551845

End: 553785

Strand: Direct

Name: iolD [H]

Synonym: KP1_0559

Alternate gene names: 238892758

Gene position: 551845-553785 (Clockwise)

Preceding gene: 238892755

Following gene: 238892759

Centisome position: 10.51

GC content: 64.14

Gene sequence:

>1941_bases
ATGGGCAAACTGAGACTGACAACGGCGCAGGCGCTGGTGAAGTTCCTCGATAACCAGTACCTGGAAGTGGATGGCGTAGA
GCTGAAGTTCGTGAAAGGCATTTTCGCGATTTTCGGCCATGGCAACGTCCTGGGGCTGGGGCAGGCGCTGGAGCAGGACA
GCGGCGATATGCGCGTCTATCAGGGGCGTAACGAGCAGGGGATGGCCCACGCGGCCACCGGTTTTGCCCGCCAGGCCCTG
CGCCGGCAGATTATCGCCTGCACCTCCTCCATCGGTCCAGGGGCGGCCAACATGATCACCGCCGCCGGCACCGCCAGCGC
CAACCGCATTCCGCTGCTGTTGCTGCCGGGCGATGTCTTCGCCACCCGCCAGCCGGACCCGGTGCTCCAGCAGATTGAAC
AGAGCTACGATCTCAGCATCAGCACCAATGACGCTTTCCGCGCAGTCAGCAAATACTGGGACCGTATTACCCGCCCGGAA
CAGCTGATGAGCGCCTGCATCAACGCCATGCGCGTCCTGACCGACCCTGCGGAAACCGGCGCGGTAACGCTGTGCCTGCC
GCAGGACGTGCAGGGCGAAGCCTGGGATTACCCGGAATCCTTCTTTGCGCGCCGCGTGCACCGTCTCGACCGCCGCCCGG
CCAGCGCCGCGCAGCTGGCGGACGCCGTGGCGGCCATCAAAGCCAGCCGTAAACCGCTGATCGTCTGCGGCGGCGGGGTG
AAATACTCCGGCGCGGGCGAAGCGCTGTCCCGTTTCGCCGAGCGCTACGGGGTGCCCTTCGCCGAAACCCAGGCCGGGAA
GGGGACGGTGGTCTCGTCTCACCCCCTGAACGTCGGCGGCGTCGGCGAGACGGGCTGCCTGGCGGCGAACCTGCTGGCGA
AAGAGGCCGACCTGGTGATCGGCGTCGGCACCCGCTTCAGCGATTTTACCACCGCCTCGAAATGGATTTTCCAGCACCCG
GAGGTGCGTTTCCTGAATATTAACGTCAGCAACTTCGACGCCTGGAAGCTGGACGGCATTGCCATGCTGGCGGACGCCCG
GGAAGCGATGACCGCTCTCGACGCGGCGCTGGCGGACAGCGGCTGGCAGGCCGGCTGGGGCGCGCAGATCGAGAGCGTGC
AGAGCCGTCAGTTAAAAGAGACCCAGCGCGTCTACCAGGCGGTGTGGCAGGAGAAATCCTTTGTGCCGGAGATCGACGAT
CATCTCGATCGCGAATCGGTGTATCGCGAGTTCCGCCAGATCACCGACTCCACCCTGACCCAGAGCAGCGTTCTTGGGGT
ACTGAATGAAACGCTGCCGGCCGAGGCGGTGATCGTCGCGGCGGCGGGCAGTCTGCCAGGCGATCTGCAGCGCGTCTGGC
GCAACCGCGCGGAAAACACCTACCACGTGGAGTACGGCTACTCCTGCATGGGATACGAAGTCAACGCCGCGCTGGGCGTG
AAGCTGGCGCAGCCGCAGAGCGAAGTCTACTCCCTGGTCGGCGATGGCTCCTTCATGATGCTGCACTCTGAGCTGGTCAC
CTCCCTGCAGGAACGGGCGAAGATCAACGTCGTGCTGTTCGACAACATGGCCAACGGCTGCATCAACAACCTGCAGATGG
AGCACGGGATGGACAGCTTCGGCACCGAGTTCCGCTACCGTCAGCCGGAAACCGGCCAGCTGCAGGGCGGCCTGGTGCCG
GTGGACTTCGCCACCATCGCCGCCGGCTACGGCTGCAAAACCTGGCGCGTCACTACTCTGGACGAACTGCGCCATGCGCT
GGACGCCGCCCGTCGCGAAACGGTCAGCACCCTGATTGATATTAAAGTACTACCGAAGACCATGGTGCACAAATACGGCA
GCTGGTGGAACGTTGGGGTCGCCCAGACGGCGCTCTCTGAGCGGATCCGCAAGGTGGCGCAAATGATTAATGAAAAGCGC
GCCCAGGCGCGTGATTACTAA

Upstream 100 bases:

>100_bases
TTTGCCTATAATCCACAGGTATTCCAGTGACGTGAACGACGGCCCCGGCGGCATCGGCGCGACGCCACACACAAATACTG
AACAATAAGGATTGTGCATC

Downstream 100 bases:

>100_bases
AACGAACCTCTACCCTGAATCCTACAGACTTACGGAGAAAACCATTATGTCGCTCAAATTAGGTGTCATCGGCGCTGGCG
CTATCGGTAAAGAACACATC

Product: acetolactate synthase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MGKLRLTTAQALVKFLDNQYLEVDGVELKFVKGIFAIFGHGNVLGLGQALEQDSGDMRVYQGRNEQGMAHAATGFARQAL
RRQIIACTSSIGPGAANMITAAGTASANRIPLLLLPGDVFATRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRITRPE
QLMSACINAMRVLTDPAETGAVTLCLPQDVQGEAWDYPESFFARRVHRLDRRPASAAQLADAVAAIKASRKPLIVCGGGV
KYSGAGEALSRFAERYGVPFAETQAGKGTVVSSHPLNVGGVGETGCLAANLLAKEADLVIGVGTRFSDFTTASKWIFQHP
EVRFLNINVSNFDAWKLDGIAMLADAREAMTALDAALADSGWQAGWGAQIESVQSRQLKETQRVYQAVWQEKSFVPEIDD
HLDRESVYREFRQITDSTLTQSSVLGVLNETLPAEAVIVAAAGSLPGDLQRVWRNRAENTYHVEYGYSCMGYEVNAALGV
KLAQPQSEVYSLVGDGSFMMLHSELVTSLQERAKINVVLFDNMANGCINNLQMEHGMDSFGTEFRYRQPETGQLQGGLVP
VDFATIAAGYGCKTWRVTTLDELRHALDAARRETVSTLIDIKVLPKTMVHKYGSWWNVGVAQTALSERIRKVAQMINEKR
AQARDY

Sequences:

>Translated_646_residues
MGKLRLTTAQALVKFLDNQYLEVDGVELKFVKGIFAIFGHGNVLGLGQALEQDSGDMRVYQGRNEQGMAHAATGFARQAL
RRQIIACTSSIGPGAANMITAAGTASANRIPLLLLPGDVFATRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRITRPE
QLMSACINAMRVLTDPAETGAVTLCLPQDVQGEAWDYPESFFARRVHRLDRRPASAAQLADAVAAIKASRKPLIVCGGGV
KYSGAGEALSRFAERYGVPFAETQAGKGTVVSSHPLNVGGVGETGCLAANLLAKEADLVIGVGTRFSDFTTASKWIFQHP
EVRFLNINVSNFDAWKLDGIAMLADAREAMTALDAALADSGWQAGWGAQIESVQSRQLKETQRVYQAVWQEKSFVPEIDD
HLDRESVYREFRQITDSTLTQSSVLGVLNETLPAEAVIVAAAGSLPGDLQRVWRNRAENTYHVEYGYSCMGYEVNAALGV
KLAQPQSEVYSLVGDGSFMMLHSELVTSLQERAKINVVLFDNMANGCINNLQMEHGMDSFGTEFRYRQPETGQLQGGLVP
VDFATIAAGYGCKTWRVTTLDELRHALDAARRETVSTLIDIKVLPKTMVHKYGSWWNVGVAQTALSERIRKVAQMINEKR
AQARDY
>Mature_645_residues
GKLRLTTAQALVKFLDNQYLEVDGVELKFVKGIFAIFGHGNVLGLGQALEQDSGDMRVYQGRNEQGMAHAATGFARQALR
RQIIACTSSIGPGAANMITAAGTASANRIPLLLLPGDVFATRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRITRPEQ
LMSACINAMRVLTDPAETGAVTLCLPQDVQGEAWDYPESFFARRVHRLDRRPASAAQLADAVAAIKASRKPLIVCGGGVK
YSGAGEALSRFAERYGVPFAETQAGKGTVVSSHPLNVGGVGETGCLAANLLAKEADLVIGVGTRFSDFTTASKWIFQHPE
VRFLNINVSNFDAWKLDGIAMLADAREAMTALDAALADSGWQAGWGAQIESVQSRQLKETQRVYQAVWQEKSFVPEIDDH
LDRESVYREFRQITDSTLTQSSVLGVLNETLPAEAVIVAAAGSLPGDLQRVWRNRAENTYHVEYGYSCMGYEVNAALGVK
LAQPQSEVYSLVGDGSFMMLHSELVTSLQERAKINVVLFDNMANGCINNLQMEHGMDSFGTEFRYRQPETGQLQGGLVPV
DFATIAAGYGCKTWRVTTLDELRHALDAARRETVSTLIDIKVLPKTMVHKYGSWWNVGVAQTALSERIRKVAQMINEKRA
QARDY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=20.3208556149733, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI21361361, Length=507, Percent_Identity=24.65483234714, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1790104, Length=607, Percent_Identity=24.2174629324547, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI87081685, Length=591, Percent_Identity=26.2267343485618, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786717, Length=580, Percent_Identity=25.6896551724138, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787096, Length=529, Percent_Identity=20.226843100189, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788716, Length=545, Percent_Identity=21.1009174311927, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323755, Length=607, Percent_Identity=23.3937397034596, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922626, Length=605, Percent_Identity=22.4793388429752, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 70757; Mature: 70626

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLRLTTAQALVKFLDNQYLEVDGVELKFVKGIFAIFGHGNVLGLGQALEQDSGDMRVY
CCCEEHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMAHAATGFARQALRRQIIACTSSIGPGAANMITAAGTASANRIPLLLLPGDVF
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCEEEEECCCEE
ATRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRITRPEQLMSACINAMRVLTDPAETG
CCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTLCLPQDVQGEAWDYPESFFARRVHRLDRRPASAAQLADAVAAIKASRKPLIVCGGGV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCE
KYSGAGEALSRFAERYGVPFAETQAGKGTVVSSHPLNVGGVGETGCLAANLLAKEADLVI
EECCHHHHHHHHHHHHCCCCHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEE
GVGTRFSDFTTASKWIFQHPEVRFLNINVSNFDAWKLDGIAMLADAREAMTALDAALADS
EECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCC
GWQAGWGAQIESVQSRQLKETQRVYQAVWQEKSFVPEIDDHLDRESVYREFRQITDSTLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
QSSVLGVLNETLPAEAVIVAAAGSLPGDLQRVWRNRAENTYHVEYGYSCMGYEVNAALGV
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCEEECEEECCEECE
KLAQPQSEVYSLVGDGSFMMLHSELVTSLQERAKINVVLFDNMANGCINNLQMEHGMDSF
EECCCHHHHHHHHCCCCEEEEHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHCCCCHH
GTEFRYRQPETGQLQGGLVPVDFATIAAGYGCKTWRVTTLDELRHALDAARRETVSTLID
CCCCEECCCCCCCCCCCEEEECHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
IKVLPKTMVHKYGSWWNVGVAQTALSERIRKVAQMINEKRAQARDY
HHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKLRLTTAQALVKFLDNQYLEVDGVELKFVKGIFAIFGHGNVLGLGQALEQDSGDMRVY
CCEEHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMAHAATGFARQALRRQIIACTSSIGPGAANMITAAGTASANRIPLLLLPGDVF
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCEEEEECCCEE
ATRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRITRPEQLMSACINAMRVLTDPAETG
CCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTLCLPQDVQGEAWDYPESFFARRVHRLDRRPASAAQLADAVAAIKASRKPLIVCGGGV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCE
KYSGAGEALSRFAERYGVPFAETQAGKGTVVSSHPLNVGGVGETGCLAANLLAKEADLVI
EECCHHHHHHHHHHHHCCCCHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEE
GVGTRFSDFTTASKWIFQHPEVRFLNINVSNFDAWKLDGIAMLADAREAMTALDAALADS
EECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCC
GWQAGWGAQIESVQSRQLKETQRVYQAVWQEKSFVPEIDDHLDRESVYREFRQITDSTLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
QSSVLGVLNETLPAEAVIVAAAGSLPGDLQRVWRNRAENTYHVEYGYSCMGYEVNAALGV
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCEEECEEECCEECE
KLAQPQSEVYSLVGDGSFMMLHSELVTSLQERAKINVVLFDNMANGCINNLQMEHGMDSF
EECCCHHHHHHHHCCCCEEEEHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHCCCCHH
GTEFRYRQPETGQLQGGLVPVDFATIAAGYGCKTWRVTTLDELRHALDAARRETVSTLID
CCCCEECCCCCCCCCCCEEEECHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
IKVLPKTMVHKYGSWWNVGVAQTALSERIRKVAQMINEKRAQARDY
HHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA