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The map label for this gene is miaE [H]

Identifier: 238892745

GI number: 238892745

Start: 532476

End: 533240

Strand: Direct

Name: miaE [H]

Synonym: KP1_0542

Alternate gene names: 238892745

Gene position: 532476-533240 (Clockwise)

Preceding gene: 238892744

Following gene: 238892750

Centisome position: 10.15

GC content: 61.7

Gene sequence:

>765_bases
ATGGATTACCCGCAGATTCTTTCCCCGATTATCAACTTCCTGCACTGCCCGACGCCGCAGGCGTGGATTGACGAAGCCCG
CAAGCCGGAAAACCTGGCGCTGCTGCTGACCGACCATATGGTGTGCGAGCTGAAAGCGGCGCAGAACGCCATGCTGCTGG
TGCGTCGCTACGTCGCGGATAAAGCTGACGCCGACGAACTGTTGGCCTGCCTGAAGCCGTACGAAGACTTCACCTACCGC
CGGGGGCCGGAGCCGGATTTTGTCGCCCTGCATAAACGGATCAACAAAAGCGCCATGCCGCAGACCGACGACCCGTGGGG
CCGCCAGCTGCTGGACAGCATGATTTTGTTGATTAAAGAGGAACTGCATCACTTCTGGCAGGTGCGGGAGATGATGCTGG
CGCGCGATATCCCCTACGTCAAAATCACCGCCAGCAACTATGCCCGCGGCCTGCGGCGCGAAGTGCGCTCGCATGAGCCG
GTGATGCTGATCGATAAGCTGATCTGCGGCGCCTATATCGAGGCCCGCTCCTGCGAACGCTTTGCCGCCCTGGCGCCGTG
GCTGGACGACGATCTGCAGAAGTTCTACCTGTCGCTGCTGCGCTCCGAGGCGCGTCACTATCAGGACTATCTGGATCTGG
CGCAGAAGATTGCCGGAGAAGATATCAGCGAGCGGGTGCGCCAGCTCGGCGAGGCGGAAGCGGCGCTGATCCTCCGTCCG
GAGGCCGAGTTCCGCTTTCACAGCGGTGTGCCGGTAGCGGCGTAA

Upstream 100 bases:

>100_bases
GAAGAGTATGTCGATGAAGACGACGATGGTGTTCGCCACTAAGTCGCAAATGTCGTAGATGGATACGGCGGCGCCTGCCG
CCGTATAGCAAGGAACCTCC

Downstream 100 bases:

>100_bases
CCCGCCGTATTCGGGCCCTGTAGCCCCGGTAAGCGCAGCGCTACCGGGGACGCCTGCGATATCGAGGCGCTACAGCGTTT
TCAGCATCCGCACTTCACAG

Product: hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MDYPQILSPIINFLHCPTPQAWIDEARKPENLALLLTDHMVCELKAAQNAMLLVRRYVADKADADELLACLKPYEDFTYR
RGPEPDFVALHKRINKSAMPQTDDPWGRQLLDSMILLIKEELHHFWQVREMMLARDIPYVKITASNYARGLRREVRSHEP
VMLIDKLICGAYIEARSCERFAALAPWLDDDLQKFYLSLLRSEARHYQDYLDLAQKIAGEDISERVRQLGEAEAALILRP
EAEFRFHSGVPVAA

Sequences:

>Translated_254_residues
MDYPQILSPIINFLHCPTPQAWIDEARKPENLALLLTDHMVCELKAAQNAMLLVRRYVADKADADELLACLKPYEDFTYR
RGPEPDFVALHKRINKSAMPQTDDPWGRQLLDSMILLIKEELHHFWQVREMMLARDIPYVKITASNYARGLRREVRSHEP
VMLIDKLICGAYIEARSCERFAALAPWLDDDLQKFYLSLLRSEARHYQDYLDLAQKIAGEDISERVRQLGEAEAALILRP
EAEFRFHSGVPVAA
>Mature_254_residues
MDYPQILSPIINFLHCPTPQAWIDEARKPENLALLLTDHMVCELKAAQNAMLLVRRYVADKADADELLACLKPYEDFTYR
RGPEPDFVALHKRINKSAMPQTDDPWGRQLLDSMILLIKEELHHFWQVREMMLARDIPYVKITASNYARGLRREVRSHEP
VMLIDKLICGAYIEARSCERFAALAPWLDDDLQKFYLSLLRSEARHYQDYLDLAQKIAGEDISERVRQLGEAEAALILRP
EAEFRFHSGVPVAA

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 29361; Mature: 29361

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYPQILSPIINFLHCPTPQAWIDEARKPENLALLLTDHMVCELKAAQNAMLLVRRYVAD
CCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHC
KADADELLACLKPYEDFTYRRGPEPDFVALHKRINKSAMPQTDDPWGRQLLDSMILLIKE
CCCHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ELHHFWQVREMMLARDIPYVKITASNYARGLRREVRSHEPVMLIDKLICGAYIEARSCER
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FAALAPWLDDDLQKFYLSLLRSEARHYQDYLDLAQKIAGEDISERVRQLGEAEAALILRP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECC
EAEFRFHSGVPVAA
CCCHHCCCCCCCCC
>Mature Secondary Structure
MDYPQILSPIINFLHCPTPQAWIDEARKPENLALLLTDHMVCELKAAQNAMLLVRRYVAD
CCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHC
KADADELLACLKPYEDFTYRRGPEPDFVALHKRINKSAMPQTDDPWGRQLLDSMILLIKE
CCCHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ELHHFWQVREMMLARDIPYVKITASNYARGLRREVRSHEPVMLIDKLICGAYIEARSCER
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FAALAPWLDDDLQKFYLSLLRSEARHYQDYLDLAQKIAGEDISERVRQLGEAEAALILRP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECC
EAEFRFHSGVPVAA
CCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]