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The map label for this gene is melA [H]

Identifier: 238892593

GI number: 238892593

Start: 379496

End: 380851

Strand: Direct

Name: melA [H]

Synonym: KP1_0371

Alternate gene names: 238892593

Gene position: 379496-380851 (Clockwise)

Preceding gene: 238892591

Following gene: 238892594

Centisome position: 7.23

GC content: 59.96

Gene sequence:

>1356_bases
GTGATGTCTGCCCCAAAAATTACTTTTATCGGCGCCGGTTCGACGGTGTTCGTCAAAAATATTCTCGGTGATGTTTTCCA
GCGCCCGGCGCTGAAATCGGCGCATATCGCGCTGATGGATATCGATGAAACCCGGCTGGAAGAGTCGCATATCGTGGTGC
GCAAGCTGATGGATTCCGTGGGCGCGAACGGCGAAATCAGCTGCCACCGCGACCGGAAAACCGCCCTGCAGGGCGCCGAT
TTTGTCGTCGTTGCCTTCCAGATTGGCGGCTATGAGCCCTGTACCGTCACCGATTTTGCAGTCTGCAAGCGCCACGGCCT
GGAGCAGACCATTGCCGATACGCTGGGGCCGGGAGGCATTATGCGCGCGCTGCGCACCATCCCGCACCTGTGGGCTATCT
GCGACGATATGAGCGAGGTCTGCCCGGACGCCACCATGCTTAACTACGTCAACCCGATGGCGATGAACACCTGGGCGATG
TACGCCCGCTACCCGCATATCAAGCAGGTGGGTCTGTGCCACTCGGTGCAAGGCACAGCGGAAGAGCTGGCGCGCGACCT
CGATCTCGATCCGGCGGATTTACGCTACCGCTGCGCGGGGATTAACCATATGGCGTTCTATCTGTCGCTGGAGAAAAAGC
TCGCGGACGGCAGCTACGTCAGTATCTACCCCGACCTGCTACAGGCCTACGCCGACGGGCGGGCGCCGAAACCGAACCTG
CACGGCAACCCGCGCTGCGAAAACATCGTCCGCTATGAAATGCTCAAAAAGCTGGGCTACTTTGTCACCGAGTCCTCGGA
GCATTTCGCGGAATACACGCCATGGTTTATCAAGCCGGGCCGCGATGACCTTCTCGCTCGCTATAAAGTGCCGCTGGATG
AATACCCTAAACGCTGCGTCGAGCAACTGGCCAACTGGCATCAGGAGCTGGAGAGCTATAAGCGCGGGGAGCGCATTGAG
GTGAAACCCTCCCGCGAATATGCCAGCACCATCATGAACGCCATCTGGACCGGCGAGCCAAGCGTGGTGTACGGCAACGT
GCGCAATGATAATCTCATCGACAACCTGCCTCAGGGCTGCTGTGTGGAAGTGGCCTGCCTGGTGGACGCCAACGGCATCC
AGCCGACCAAAGTGGGCGCGCTGCCGGCGCATCTGGCGGCGCTGATGCAGACCAATATCAACGTGCAGACGCTGCTCACG
CAGGCGATCCTCACCGAAAATCGCGATTACGTCTATTACGCCACGATGATGGATCCGCACACCGCCGCGGTGCTGGGGAT
CGAAGAGATCTACGCGCTGGTGGACGATCTGATCGCCAGCCACGGCGACTGGCTGCCGGCCTGGCTGCACCGTTAA

Upstream 100 bases:

>100_bases
GGCCGATCTGAGCTTTCGGGAATGCTCCGGCGGGAACAGGGCAGCGCAGCGGGGAGCGGCGTGCCACTCTGCAAGTGTTC
AATGACCTGATGGAGAGTGC

Downstream 100 bases:

>100_bases
TCCTCTTATGCCGCCGGGGGCAACGAACCCGGCGGTGCCTGTCACGCTCTATCAAACGCTGAAGGAGGCGCTGGCGACAG
CGACCTGGACCCTATGAGTA

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MMSAPKITFIGAGSTVFVKNILGDVFQRPALKSAHIALMDIDETRLEESHIVVRKLMDSVGANGEISCHRDRKTALQGAD
FVVVAFQIGGYEPCTVTDFAVCKRHGLEQTIADTLGPGGIMRALRTIPHLWAICDDMSEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLDLDPADLRYRCAGINHMAFYLSLEKKLADGSYVSIYPDLLQAYADGRAPKPNL
HGNPRCENIVRYEMLKKLGYFVTESSEHFAEYTPWFIKPGRDDLLARYKVPLDEYPKRCVEQLANWHQELESYKRGERIE
VKPSREYASTIMNAIWTGEPSVVYGNVRNDNLIDNLPQGCCVEVACLVDANGIQPTKVGALPAHLAALMQTNINVQTLLT
QAILTENRDYVYYATMMDPHTAAVLGIEEIYALVDDLIASHGDWLPAWLHR

Sequences:

>Translated_451_residues
MMSAPKITFIGAGSTVFVKNILGDVFQRPALKSAHIALMDIDETRLEESHIVVRKLMDSVGANGEISCHRDRKTALQGAD
FVVVAFQIGGYEPCTVTDFAVCKRHGLEQTIADTLGPGGIMRALRTIPHLWAICDDMSEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLDLDPADLRYRCAGINHMAFYLSLEKKLADGSYVSIYPDLLQAYADGRAPKPNL
HGNPRCENIVRYEMLKKLGYFVTESSEHFAEYTPWFIKPGRDDLLARYKVPLDEYPKRCVEQLANWHQELESYKRGERIE
VKPSREYASTIMNAIWTGEPSVVYGNVRNDNLIDNLPQGCCVEVACLVDANGIQPTKVGALPAHLAALMQTNINVQTLLT
QAILTENRDYVYYATMMDPHTAAVLGIEEIYALVDDLIASHGDWLPAWLHR
>Mature_451_residues
MMSAPKITFIGAGSTVFVKNILGDVFQRPALKSAHIALMDIDETRLEESHIVVRKLMDSVGANGEISCHRDRKTALQGAD
FVVVAFQIGGYEPCTVTDFAVCKRHGLEQTIADTLGPGGIMRALRTIPHLWAICDDMSEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLDLDPADLRYRCAGINHMAFYLSLEKKLADGSYVSIYPDLLQAYADGRAPKPNL
HGNPRCENIVRYEMLKKLGYFVTESSEHFAEYTPWFIKPGRDDLLARYKVPLDEYPKRCVEQLANWHQELESYKRGERIE
VKPSREYASTIMNAIWTGEPSVVYGNVRNDNLIDNLPQGCCVEVACLVDANGIQPTKVGALPAHLAALMQTNINVQTLLT
QAILTENRDYVYYATMMDPHTAAVLGIEEIYALVDDLIASHGDWLPAWLHR

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=451, Percent_Identity=87.3614190687361, Blast_Score=848, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=462, Percent_Identity=25.3246753246753, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50497; Mature: 50497

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAPKITFIGAGSTVFVKNILGDVFQRPALKSAHIALMDIDETRLEESHIVVRKLMDSV
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
GANGEISCHRDRKTALQGADFVVVAFQIGGYEPCTVTDFAVCKRHGLEQTIADTLGPGGI
CCCCCEEECCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWAICDDMSEVCPDATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLDLDPADLRYRCAGINHMAFYLSLEKKLADGSYVSIYPDLLQAYADGRAPKPNL
HHHHHHCCCCHHHCEEEECCCCEEEEEEEHHHHHCCCCEEEECHHHHHHHHCCCCCCCCC
HGNPRCENIVRYEMLKKLGYFVTESSEHFAEYTPWFIKPGRDDLLARYKVPLDEYPKRCV
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCCHHHHHHHCCCCHHHHHHHHH
EQLANWHQELESYKRGERIEVKPSREYASTIMNAIWTGEPSVVYGNVRNDNLIDNLPQGC
HHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHCCCCH
CVEVACLVDANGIQPTKVGALPAHLAALMQTNINVQTLLTQAILTENRDYVYYATMMDPH
HEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEECCC
TAAVLGIEEIYALVDDLIASHGDWLPAWLHR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure
MMSAPKITFIGAGSTVFVKNILGDVFQRPALKSAHIALMDIDETRLEESHIVVRKLMDSV
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
GANGEISCHRDRKTALQGADFVVVAFQIGGYEPCTVTDFAVCKRHGLEQTIADTLGPGGI
CCCCCEEECCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWAICDDMSEVCPDATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLDLDPADLRYRCAGINHMAFYLSLEKKLADGSYVSIYPDLLQAYADGRAPKPNL
HHHHHHCCCCHHHCEEEECCCCEEEEEEEHHHHHCCCCEEEECHHHHHHHHCCCCCCCCC
HGNPRCENIVRYEMLKKLGYFVTESSEHFAEYTPWFIKPGRDDLLARYKVPLDEYPKRCV
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCCHHHHHHHCCCCHHHHHHHHH
EQLANWHQELESYKRGERIEVKPSREYASTIMNAIWTGEPSVVYGNVRNDNLIDNLPQGC
HHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHCCCCH
CVEVACLVDANGIQPTKVGALPAHLAALMQTNINVQTLLTQAILTENRDYVYYATMMDPH
HEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEECCC
TAAVLGIEEIYALVDDLIASHGDWLPAWLHR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]