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The map label for this gene is melB [H]

Identifier: 238892594

GI number: 238892594

Start: 380945

End: 382360

Strand: Direct

Name: melB [H]

Synonym: KP1_0372

Alternate gene names: 238892594

Gene position: 380945-382360 (Clockwise)

Preceding gene: 238892593

Following gene: 238892595

Centisome position: 7.26

GC content: 55.23

Gene sequence:

>1416_bases
ATGAGTATTTCAATGACAACAAAGCTCAGCTACGGATTTGGTGCCTTTGGTAAAGATTTCGCTATCGGTATCGTCTACAT
GTACCTGATGTATTACTACACCGATATTGTTGGCTTATCGGTTGGGGTGGTTGGCACCTTGTTCCTGGTGGCAAGGATCC
TCGATGCGATAGCCGATCCGATTATGGGATGGATCGTTAACTGTACGCGATCGCGATGGGGGAAGTTCAAACCGTGGATC
CTGATCGGCACGATAACCAACTCGGTGGTGCTGTATATGCTGTTCAGCGCCCACCATTTCAGCGGCGGGGCGCTGCTGGC
GTGGGTGTGGCTAACCTACCTGCTGTGGGGCTTCACTTACACCATCATGGATGTGCCGTTCTGGTCGTTGGTGCCGACCA
TTACCCTCGATAAACGCGAACGCGAGCAGCTGGTGCCTTACCCGCGCTTTTTCGCCAGTCTGGCGGGATTTGTCACGGCC
GGCGTCACGCTGCCGTTCGTCAACGCCGTCGGCGGCGCCGATCGCGGTTTCGGTTTTCAGATGTTCACTCTGGTGCTGAT
CGCCTTCTTCGTCGTTTCCACCCTCGTCACCCTGCGCAACGTGCATGAGGTTTACTCCTCCGACAGTGGTGTGAGCGAAG
ACTCCAGCCACCTCTCACTGCGCCAGATGGTGGCGCTGATCTATAAAAACGATCAGCTGGCCTGCCTGTTAGGCATGGCG
CTGGCCTATAACACGGCGGCGAACATTATCGCTGGCTTTGCGATTTACTACTTTACCTACGTGATTGGCAGCGCGGAGAT
GTTCCCGTATTACATGTCCTACGCCGGGGCGGCTAATTTGCTGACGCTGATCCTGTTCCCACGACTGGTGAAAGGGCTGT
CGCGGCGCATTCTGTGGGCGGGTGCTTCGATCATGCCCGTGTTGGGCTGCGGCGTGCTGTTGCTGATGGCGCTGGGCGGC
GTTTACAACATCGCGCTGATTTCGCTGGCCGGGATGCTGTTGAATATCGGCACCGCGCTGTTCTGGGTGCTGCAGGTTAT
TATGGTGGCCGATACCGTCGACTATGGCGAATATACGATGAATATCCGCTGCGAAAGCATCGCCTATTCCGTTCAAACGC
TGGTAGTCAAAGCCGGTTCGGCGTTTGCGGCCTGGTTTATCGCCATCGTGCTGGGTATCATCGGTTATGTGCCGAATGTT
GTGCAGTCGCCCCACACGCTGCTGGGGATGCAGGCGATTATGATTGCGCTGCCGACGCTCTTTTTTGCTCTGACGCTGTT
TCTCTATTTCCGTTATTACAAACTGAATGGCGATATGCTGCGACGGATTCAGATTCACCTGCTGGATAAATATCGCCGGG
TACCGGAAAATGACGTCGAGCCGGAACGGCCGATTGTGGTACCCAATCAGGTGTAA

Upstream 100 bases:

>100_bases
CCGTTAATCCTCTTATGCCGCCGGGGGCAACGAACCCGGCGGTGCCTGTCACGCTCTATCAAACGCTGAAGGAGGCGCTG
GCGACAGCGACCTGGACCCT

Downstream 100 bases:

>100_bases
AAAGCGTGCGAAAACCGCTGACGAAAGAGGGGAGGGATGCTCTCTTTTTGAGCAAAAAACAGTGTCTTAAAAAGACAGAA
AGAGAAAACTGTAGTTCTCT

Product: melibiose:sodium symporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Melibiose permease; Melibiose transporter; Na+ (Li+)/melibiose symporter; Thiomethylgalactoside permease II [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDIVGLSVGVVGTLFLVARILDAIADPIMGWIVNCTRSRWGKFKPWI
LIGTITNSVVLYMLFSAHHFSGGALLAWVWLTYLLWGFTYTIMDVPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
GVTLPFVNAVGGADRGFGFQMFTLVLIAFFVVSTLVTLRNVHEVYSSDSGVSEDSSHLSLRQMVALIYKNDQLACLLGMA
LAYNTAANIIAGFAIYYFTYVIGSAEMFPYYMSYAGAANLLTLILFPRLVKGLSRRILWAGASIMPVLGCGVLLLMALGG
VYNIALISLAGMLLNIGTALFWVLQVIMVADTVDYGEYTMNIRCESIAYSVQTLVVKAGSAFAAWFIAIVLGIIGYVPNV
VQSPHTLLGMQAIMIALPTLFFALTLFLYFRYYKLNGDMLRRIQIHLLDKYRRVPENDVEPERPIVVPNQV

Sequences:

>Translated_471_residues
MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDIVGLSVGVVGTLFLVARILDAIADPIMGWIVNCTRSRWGKFKPWI
LIGTITNSVVLYMLFSAHHFSGGALLAWVWLTYLLWGFTYTIMDVPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
GVTLPFVNAVGGADRGFGFQMFTLVLIAFFVVSTLVTLRNVHEVYSSDSGVSEDSSHLSLRQMVALIYKNDQLACLLGMA
LAYNTAANIIAGFAIYYFTYVIGSAEMFPYYMSYAGAANLLTLILFPRLVKGLSRRILWAGASIMPVLGCGVLLLMALGG
VYNIALISLAGMLLNIGTALFWVLQVIMVADTVDYGEYTMNIRCESIAYSVQTLVVKAGSAFAAWFIAIVLGIIGYVPNV
VQSPHTLLGMQAIMIALPTLFFALTLFLYFRYYKLNGDMLRRIQIHLLDKYRRVPENDVEPERPIVVPNQV
>Mature_470_residues
SISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDIVGLSVGVVGTLFLVARILDAIADPIMGWIVNCTRSRWGKFKPWIL
IGTITNSVVLYMLFSAHHFSGGALLAWVWLTYLLWGFTYTIMDVPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAG
VTLPFVNAVGGADRGFGFQMFTLVLIAFFVVSTLVTLRNVHEVYSSDSGVSEDSSHLSLRQMVALIYKNDQLACLLGMAL
AYNTAANIIAGFAIYYFTYVIGSAEMFPYYMSYAGAANLLTLILFPRLVKGLSRRILWAGASIMPVLGCGVLLLMALGGV
YNIALISLAGMLLNIGTALFWVLQVIMVADTVDYGEYTMNIRCESIAYSVQTLVVKAGSAFAAWFIAIVLGIIGYVPNVV
QSPHTLLGMQAIMIALPTLFFALTLFLYFRYYKLNGDMLRRIQIHLLDKYRRVPENDVEPERPIVVPNQV

Specific function: Responsible for melibiose transport. It is capable of using hydrogen and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system) [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=461, Percent_Identity=79.6095444685466, Blast_Score=761, Evalue=0.0,
Organism=Escherichia coli, GI87082306, Length=465, Percent_Identity=28.8172043010753, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786466, Length=477, Percent_Identity=28.5115303983228, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787902, Length=464, Percent_Identity=26.9396551724138, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI48994989, Length=465, Percent_Identity=23.6559139784946, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI145693206, Length=454, Percent_Identity=25.1101321585903, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52372; Mature: 52241

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDIVGLSVGVVGTLFLVARILDAIADP
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMGWIVNCTRSRWGKFKPWILIGTITNSVVLYMLFSAHHFSGGALLAWVWLTYLLWGFTY
HHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TIMDVPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLPFVNAVGGADRGFGFQ
HHHHCCHHHHCCCEEECCHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHH
MFTLVLIAFFVVSTLVTLRNVHEVYSSDSGVSEDSSHLSLRQMVALIYKNDQLACLLGMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LAYNTAANIIAGFAIYYFTYVIGSAEMFPYYMSYAGAANLLTLILFPRLVKGLSRRILWA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASIMPVLGCGVLLLMALGGVYNIALISLAGMLLNIGTALFWVLQVIMVADTVDYGEYTM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
NIRCESIAYSVQTLVVKAGSAFAAWFIAIVLGIIGYVPNVVQSPHTLLGMQAIMIALPTL
EEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHH
FFALTLFLYFRYYKLNGDMLRRIQIHLLDKYRRVPENDVEPERPIVVPNQV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure 
SISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDIVGLSVGVVGTLFLVARILDAIADP
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMGWIVNCTRSRWGKFKPWILIGTITNSVVLYMLFSAHHFSGGALLAWVWLTYLLWGFTY
HHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TIMDVPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLPFVNAVGGADRGFGFQ
HHHHCCHHHHCCCEEECCHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHH
MFTLVLIAFFVVSTLVTLRNVHEVYSSDSGVSEDSSHLSLRQMVALIYKNDQLACLLGMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LAYNTAANIIAGFAIYYFTYVIGSAEMFPYYMSYAGAANLLTLILFPRLVKGLSRRILWA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASIMPVLGCGVLLLMALGGVYNIALISLAGMLLNIGTALFWVLQVIMVADTVDYGEYTM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
NIRCESIAYSVQTLVVKAGSAFAAWFIAIVLGIIGYVPNVVQSPHTLLGMQAIMIALPTL
EEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHH
FFALTLFLYFRYYKLNGDMLRRIQIHLLDKYRRVPENDVEPERPIVVPNQV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9188803 [H]