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The map label for this gene is proP [H]

Identifier: 238892591

GI number: 238892591

Start: 376769

End: 378271

Strand: Direct

Name: proP [H]

Synonym: KP1_0369

Alternate gene names: 238892591

Gene position: 376769-378271 (Clockwise)

Preceding gene: 238892578

Following gene: 238892593

Centisome position: 7.18

GC content: 55.89

Gene sequence:

>1503_bases
ATGCTTAAAAGGAAAAAAATAAAGCCCATCACCCTTAAGGATGTGACCATCATCGATGATGGTAAACTGAAAAAGGCGAT
CACCGCGGCCTCCCTGGGCAACGCGATGGAGTGGTTCGATTTCGGCGTCTATGGCTTTGTGGCCTACGCCTTAGGTAAAG
TCTTCTTCCCCGATGCCAACCCAAGCGTGCAGATGATTGCCGCGCTGGGTACCTTCTCCGTACCATTTTTAATTCGTCCG
TTAGGCGGCCTGTTCTTCGGGATGCTGGGCGATAAATATGGTCGGCAAAAAATTCTCGCGATAACTATCGTGATCATGTC
GATAAGTACTTTCTGTATCGGCCTTATTCCGTCGTATGCCACGATAGGTATCTGGGCGCCGATCCTGCTGCTGCTGTGTA
AAATGGCGCAAGGCTTCTCGGTCGGCGGTGAATATACCGGGGCGTCGATCTTCGTCGCGGAATACTCTCCTGACCGCAAG
CGCGGCTTTATGGGCAGCTGGCTGGACTTTGGCTCCATCGCCGGGTTTGTGATGGGGGCCGGCGTGGTGGTTCTCATCTC
CAGCGTGGTGGGTGAACAGAACTTTCTCGACTGGGGTTGGCGTATCCCGTTCTTCCTGGCGCTACCGTTAGGTATCATTG
GTCTCTACCTGCGCCATGCGCTGGAAGAGACGCCGGCGTTCCAGCAGCATGTTGACACCATGGAGCAGGGCGACCGTGAA
GGACTGCAGGACGGGCCGAAGGTCTCTTTTAAAGAGATTGCCACCAAGCACTGGCGTAGCCTGCTGACCTGTATCGGTCT
GGTGATTTCCACCAACGTGACTTACTACATGCTGCTGACCTACATGCCGAGCTATCTGTCGCATAACCTGCACTACTCGG
AAGATCATGGGGTGCTGATCATCATCGCTATCATGGTCGGGATGCTGTTTGTACAGCCGGTTATCGGTATGCTCAGCGAC
CGTTTCGGCCGTCGCCCGTTTATCCTGATTGGTAGCGTGGCGCTGTTTGCCCTGTCGATCCCGGCCTTCATTATGATCAA
CAGCAACGTGACTAGCCTGATCTTCGCCGGCCTGCTGCTGCTGGCGGTGGTGCTGAACTGCTTTATCGGGGTGATGGCCT
CCTCGCTGCCGGCGATGTTCCCGACGCACATCCGCTTCAGCGCCCTGGCCAGCGCCTTTAACATCTCGGTGCTGATTGCC
GGTCTGACGCCAACCCTGGCGGCGTGGCTGGTGGAGACCACCCAAAACCTGATGATGCCGGCGTACTACCTGATGGTTAT
CGCGGTGGTGGGTATGGCGACGGGCCTGTCGATGAAAGAGACGGCTAACCGTCCGCTGAAAGGGGCGACCCCGGCGGCGT
CGGATATCCAGGAAGCGAAAGAAATCCTTGGCGAGCACTACGATAACATCGAGCAAAAAATCGAAGATATCGATCAGGAG
ATCGCCGATCTGCAGGCAAAACGGTCGCGTCTGGTGCAGCAGCATCCGCGTATCAACGACTAA

Upstream 100 bases:

>100_bases
ATGAGGAGCACAGCAATTCACCGCTCCTGACGCACGATGCGAATGGCCTTGCCATTCGGCGAAGAGGCGTGCACTCAAGG
CGGAGTAGCAGAGGAAAAAT

Downstream 100 bases:

>100_bases
CCGTTTTCACTGTTAATCATGCCGCCTGGGGAAACCCCGGCGGCATTTTTTATGCCTTCGGCAAGGCCAGGCCGTGACGC
CGCTGCTGGCTGAGTTTACG

Product: proline/glycine betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MLKRKKIKPITLKDVTIIDDGKLKKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPDANPSVQMIAALGTFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYATIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVMGAGVVVLISSVVGEQNFLDWGWRIPFFLALPLGIIGLYLRHALEETPAFQQHVDTMEQGDRE
GLQDGPKVSFKEIATKHWRSLLTCIGLVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMVGMLFVQPVIGMLSD
RFGRRPFILIGSVALFALSIPAFIMINSNVTSLIFAGLLLLAVVLNCFIGVMASSLPAMFPTHIRFSALASAFNISVLIA
GLTPTLAAWLVETTQNLMMPAYYLMVIAVVGMATGLSMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIEDIDQE
IADLQAKRSRLVQQHPRIND

Sequences:

>Translated_500_residues
MLKRKKIKPITLKDVTIIDDGKLKKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPDANPSVQMIAALGTFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYATIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVMGAGVVVLISSVVGEQNFLDWGWRIPFFLALPLGIIGLYLRHALEETPAFQQHVDTMEQGDRE
GLQDGPKVSFKEIATKHWRSLLTCIGLVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMVGMLFVQPVIGMLSD
RFGRRPFILIGSVALFALSIPAFIMINSNVTSLIFAGLLLLAVVLNCFIGVMASSLPAMFPTHIRFSALASAFNISVLIA
GLTPTLAAWLVETTQNLMMPAYYLMVIAVVGMATGLSMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIEDIDQE
IADLQAKRSRLVQQHPRIND
>Mature_500_residues
MLKRKKIKPITLKDVTIIDDGKLKKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPDANPSVQMIAALGTFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYATIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVMGAGVVVLISSVVGEQNFLDWGWRIPFFLALPLGIIGLYLRHALEETPAFQQHVDTMEQGDRE
GLQDGPKVSFKEIATKHWRSLLTCIGLVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMVGMLFVQPVIGMLSD
RFGRRPFILIGSVALFALSIPAFIMINSNVTSLIFAGLLLLAVVLNCFIGVMASSLPAMFPTHIRFSALASAFNISVLIA
GLTPTLAAWLVETTQNLMMPAYYLMVIAVVGMATGLSMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIEDIDQE
IADLQAKRSRLVQQHPRIND

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=500, Percent_Identity=90.4, Blast_Score=951, Evalue=0.0,
Organism=Escherichia coli, GI1788292, Length=443, Percent_Identity=31.1512415349887, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=32.7906976744186, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=31.554524361949, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87082231, Length=486, Percent_Identity=23.4567901234568, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082404, Length=391, Percent_Identity=23.7851662404092, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 54802; Mature: 54802

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRKKIKPITLKDVTIIDDGKLKKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPDAN
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PSVQMIAALGTFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYA
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVMGA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHH
GVVVLISSVVGEQNFLDWGWRIPFFLALPLGIIGLYLRHALEETPAFQQHVDTMEQGDRE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
GLQDGPKVSFKEIATKHWRSLLTCIGLVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IIAIMVGMLFVQPVIGMLSDRFGRRPFILIGSVALFALSIPAFIMINSNVTSLIFAGLLL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCEEEEECCCHHHHHHHHHHH
LAVVLNCFIGVMASSLPAMFPTHIRFSALASAFNISVLIAGLTPTLAAWLVETTQNLMMP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHH
AYYLMVIAVVGMATGLSMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIEDIDQE
HHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADLQAKRSRLVQQHPRIND
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKRKKIKPITLKDVTIIDDGKLKKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPDAN
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PSVQMIAALGTFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYA
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVMGA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHH
GVVVLISSVVGEQNFLDWGWRIPFFLALPLGIIGLYLRHALEETPAFQQHVDTMEQGDRE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
GLQDGPKVSFKEIATKHWRSLLTCIGLVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IIAIMVGMLFVQPVIGMLSDRFGRRPFILIGSVALFALSIPAFIMINSNVTSLIFAGLLL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCEEEEECCCHHHHHHHHHHH
LAVVLNCFIGVMASSLPAMFPTHIRFSALASAFNISVLIAGLTPTLAAWLVETTQNLMMP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHH
AYYLMVIAVVGMATGLSMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIEDIDQE
HHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADLQAKRSRLVQQHPRIND
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]