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The map label for this gene is nuoM [H]

Identifier: 238028131

GI number: 238028131

Start: 2943952

End: 2945445

Strand: Reverse

Name: nuoM [H]

Synonym: bglu_1g25820

Alternate gene names: 238028131

Gene position: 2945445-2943952 (Counterclockwise)

Preceding gene: 238028132

Following gene: 238028130

Centisome position: 75.4

GC content: 61.78

Gene sequence:

>1494_bases
ATGCCCGCATTTCCGATTCTCAGTACCGCCATCTGGCTTCCGATCGTGTTCGGCCTCGTGATTCTGGCCATCGGCACGGA
CCGGAAGCCCGGCGTGATCCGTTGGGTTGCGCTGCTCGGTTCGCTGCTCGGGTTTGCTGTCACGATTCCGCTCGTCACCG
GTTTCGATCCGAGCACCGCGGCTCTCCAGTTCCAGGAGAACGTGAGCTGGATCGAACGGTTCAATGTTTCGTATCACCTC
GGCGTCGACGGGATCTCGATGTGGTTCGTCGTGCTGACGGCGCTGATCACCGTGATCGTCGTCGTTGCCGCCTGGGAAGT
CATCACCGAGAACGTCTCGCAATATCTGGCCGCGTTCCTGATCCTGTCCGGCATCATGATCGGGGTGTTTTCGGCGGCCG
ACGGCCTGCTGTTCTACGTGTTCTTTGAGGCCACGCTGATTCCGATGTACATCATCATCGGCGTCTGGGGCGGCCCGAAC
CGCGTCTACGCGGCGTTCAAGTTCTTCCTCTACACGCTGGCCGGCTCGCTGCTGATGCTGGTCGCGCTGATCTACCTGTA
CCGCGAGACGCACACCTTCGATCTGGCCGTCTGGCAGGGCGCGAAGCTCGCGATGACGCCGCAGTTGCTGCTGTTCGCCG
CGTTCTTCCTCGCCTTCGCGGTGAAGGTGCCGATGTGGCCCGTCCACACCTGGCTGCCCGACGCGCACGTCGAGGCGCCC
ACCGGCGGCTCGGTGGTGCTGGCCGCGATCATGCTCAAGCTCGGCGCCTACGGCTTCCTGCGCTTCTCGCTGCCGGTCAC
GCCCGATGCCTCGCATTATCTGGCTCCGGTCGTGATCGCCTTGTCACTCGTCGCCGTGATCTACATCGGCCTGGTGGCGA
TGGTGCAGACCGATATGAAGAAGCTGGTCGCCTACTCGTCGATCGCGCACATGGGCTTCGTCACGCTCGGCTTCTTCATC
TTCAGTCCGCTCGGTGTCGAGGGCGCGATCGTGCAGATGATTTCGCACGGCTTCGTTTCGGGGGCGATGTTTCTTTGCAT
CGGCGTGTTGTACGATCGCCTCCATACGCGCAATATCGCCGACTACGGCGGAGTCGTGAACGTCATGCCGAAGTTCGCGA
CGTTCGCGTTGCTGTTCGCGATGGCGAACTGCGGGCTGCCCGGCACCTCCGGGTTCGTCGGCGAGTTCATGGTGATCCTT
GCGTCCGTGAAGTTCAATTTCTGGATCGCGTTCGGCGCGGGCTTCACGCTGATTCTTGGCGCGGGCTACACGCTGTGGAT
GTACAAACGCGTCTATTTCGGCAAGGTGGTCAACGAGAAGGTAACGCGGCTGAAGGACATCGGCGCTCGTGAGTTCTCGA
TGCTCGCCGTGCTGGCCGCGTTCACGCTGCTGATGGGCCTGTATCCGAAGCCCTTCACCGACGTGATGCACGTTTCCGTA
GAAAACCTCCTCTCTCACGTCGCGCAATCCAAGCTGCCGCTGTCCCCGCAGTAA

Upstream 100 bases:

>100_bases
ACATTTATCACTACGCGTTCGCGATGATCATTGGCATGTTGGGTCTCCTCACCCTGTTTGTTACGCTCGGCGGCAAATAA
GAAAGGAGAGGAACCCACTC

Downstream 100 bases:

>100_bases
GGGTGGGCGGAGGAAACCAAGACCATGCAAAACGCACCTATGAATGCCTTGTTGCCCGACGCGCTCGTGATGTTCGCCAT
CATCGTGGCGTGGCTGAACG

Product: NADH:ubiquinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MPAFPILSTAIWLPIVFGLVILAIGTDRKPGVIRWVALLGSLLGFAVTIPLVTGFDPSTAALQFQENVSWIERFNVSYHL
GVDGISMWFVVLTALITVIVVVAAWEVITENVSQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPN
RVYAAFKFFLYTLAGSLLMLVALIYLYRETHTFDLAVWQGAKLAMTPQLLLFAAFFLAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPVTPDASHYLAPVVIALSLVAVIYIGLVAMVQTDMKKLVAYSSIAHMGFVTLGFFI
FSPLGVEGAIVQMISHGFVSGAMFLCIGVLYDRLHTRNIADYGGVVNVMPKFATFALLFAMANCGLPGTSGFVGEFMVIL
ASVKFNFWIAFGAGFTLILGAGYTLWMYKRVYFGKVVNEKVTRLKDIGAREFSMLAVLAAFTLLMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLSPQ

Sequences:

>Translated_497_residues
MPAFPILSTAIWLPIVFGLVILAIGTDRKPGVIRWVALLGSLLGFAVTIPLVTGFDPSTAALQFQENVSWIERFNVSYHL
GVDGISMWFVVLTALITVIVVVAAWEVITENVSQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPN
RVYAAFKFFLYTLAGSLLMLVALIYLYRETHTFDLAVWQGAKLAMTPQLLLFAAFFLAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPVTPDASHYLAPVVIALSLVAVIYIGLVAMVQTDMKKLVAYSSIAHMGFVTLGFFI
FSPLGVEGAIVQMISHGFVSGAMFLCIGVLYDRLHTRNIADYGGVVNVMPKFATFALLFAMANCGLPGTSGFVGEFMVIL
ASVKFNFWIAFGAGFTLILGAGYTLWMYKRVYFGKVVNEKVTRLKDIGAREFSMLAVLAAFTLLMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLSPQ
>Mature_496_residues
PAFPILSTAIWLPIVFGLVILAIGTDRKPGVIRWVALLGSLLGFAVTIPLVTGFDPSTAALQFQENVSWIERFNVSYHLG
VDGISMWFVVLTALITVIVVVAAWEVITENVSQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPNR
VYAAFKFFLYTLAGSLLMLVALIYLYRETHTFDLAVWQGAKLAMTPQLLLFAAFFLAFAVKVPMWPVHTWLPDAHVEAPT
GGSVVLAAIMLKLGAYGFLRFSLPVTPDASHYLAPVVIALSLVAVIYIGLVAMVQTDMKKLVAYSSIAHMGFVTLGFFIF
SPLGVEGAIVQMISHGFVSGAMFLCIGVLYDRLHTRNIADYGGVVNVMPKFATFALLFAMANCGLPGTSGFVGEFMVILA
SVKFNFWIAFGAGFTLILGAGYTLWMYKRVYFGKVVNEKVTRLKDIGAREFSMLAVLAAFTLLMGLYPKPFTDVMHVSVE
NLLSHVAQSKLPLSPQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=368, Percent_Identity=35.054347826087, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI251831117, Length=403, Percent_Identity=23.5732009925558, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1788613, Length=483, Percent_Identity=38.7163561076605, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI1788827, Length=286, Percent_Identity=26.5734265734266, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788831, Length=316, Percent_Identity=25.9493670886076, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788829, Length=337, Percent_Identity=30.2670623145401, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788614, Length=383, Percent_Identity=27.4151436031332, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693160, Length=427, Percent_Identity=22.9508196721311, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54263; Mature: 54131

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFPILSTAIWLPIVFGLVILAIGTDRKPGVIRWVALLGSLLGFAVTIPLVTGFDPSTA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ALQFQENVSWIERFNVSYHLGVDGISMWFVVLTALITVIVVVAAWEVITENVSQYLAAFL
HHEEHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLAGSLLML
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALIYLYRETHTFDLAVWQGAKLAMTPQLLLFAAFFLAFAVKVPMWPVHTWLPDAHVEAP
HHHHHHHHHCCEEEEEEECCCCEECCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
TGGSVVLAAIMLKLGAYGFLRFSLPVTPDASHYLAPVVIALSLVAVIYIGLVAMVQTDMK
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVAYSSIAHMGFVTLGFFIFSPLGVEGAIVQMISHGFVSGAMFLCIGVLYDRLHTRNIA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DYGGVVNVMPKFATFALLFAMANCGLPGTSGFVGEFMVILASVKFNFWIAFGAGFTLILG
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHH
AGYTLWMYKRVYFGKVVNEKVTRLKDIGAREFSMLAVLAAFTLLMGLYPKPFTDVMHVSV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ENLLSHVAQSKLPLSPQ
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PAFPILSTAIWLPIVFGLVILAIGTDRKPGVIRWVALLGSLLGFAVTIPLVTGFDPSTA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ALQFQENVSWIERFNVSYHLGVDGISMWFVVLTALITVIVVVAAWEVITENVSQYLAAFL
HHEEHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLAGSLLML
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALIYLYRETHTFDLAVWQGAKLAMTPQLLLFAAFFLAFAVKVPMWPVHTWLPDAHVEAP
HHHHHHHHHCCEEEEEEECCCCEECCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
TGGSVVLAAIMLKLGAYGFLRFSLPVTPDASHYLAPVVIALSLVAVIYIGLVAMVQTDMK
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVAYSSIAHMGFVTLGFFIFSPLGVEGAIVQMISHGFVSGAMFLCIGVLYDRLHTRNIA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DYGGVVNVMPKFATFALLFAMANCGLPGTSGFVGEFMVILASVKFNFWIAFGAGFTLILG
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHH
AGYTLWMYKRVYFGKVVNEKVTRLKDIGAREFSMLAVLAAFTLLMGLYPKPFTDVMHVSV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ENLLSHVAQSKLPLSPQ
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]