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The map label for this gene is odhL [H]

Identifier: 238027574

GI number: 238027574

Start: 2177577

End: 2179007

Strand: Reverse

Name: odhL [H]

Synonym: bglu_1g19920

Alternate gene names: 238027574

Gene position: 2179007-2177577 (Counterclockwise)

Preceding gene: 238027575

Following gene: 238027573

Centisome position: 55.78

GC content: 65.34

Gene sequence:

>1431_bases
ATGTCCAAGGAATTTGACGTCGTCGTGATCGGCGCCGGCCCCGGCGGCTATGTGGCCGCGATTCGCGCAGCCCAGCTCGG
CAAGACGGTGGCGGTGGTCGAGAAGTGGAAGAACCCGGCGGGCGCGCTGAAGCTCGGCGGCACCTGCCTGAACGTCGGCT
GCATCCCGTCGAAGGCGCTGCTCGCCTCGTCGGAAGAGTTCGAAAACGCGTCGCATCATCTGGCCGACCACGGCATTTCG
GTTGGTGACGTGAAGATGGATACCGCGAAGATGCTGAGCCGCAAGGACGGCATCGTCGAGAAGATGACGGGCGGGATCGA
ATTCCTGTTCAAGAAGAACAAGATCACCTGGCTCAAGGGCCATGGCAAGTTCACCGGCAAGACCGACGCCGGCGTGCAGA
TCGAGGTCAGCGGCGAAGGCGAGACGGAAGTCGTCACGGCCAGGAACGTGATCATCGCGACGGGTTCGAAGGCGCGTCAC
CTGCCGAACGTGCCGGTCGACAACAGGATCGTCGCCGACAACGAAGGCGCGCTGAACTTCGACACGGTGCCGAACAAGCT
CGCCGTGATCGGCGCGGGCGTGATCGGCCTCGAGCTCGGCTCGGTGTGGCGCCGCCTCGGCGCCGACGTGACGGTGCTCG
AAGCGATGCCGGCGTTTCTCGCCGCCGCCGACGAGGCGGTCGCCAAGGAAGCCGCGAAGCTGTTCAAGAAGCAGGGCCTC
GACATCAACCTCGGCGTGACGATCGGCGAGGTGAAGGCCAGCGATGCCGGTGTGTCGATCGCGTACACGGACAAGGACGG
CAACGCCAAGACGCTCGAGGCGGACCGTCTGATCGTGTCGGTCGGCCGCGTGCCGAACACCGACAACCTCGGCCTGGAGT
CGATCGGCCTGAAGGCCAACGAGCGCGGCTTCATCGACGTGGACGATCACTGCCGCACCGCGCTGCCGAACGTCTACGCG
ATCGGCGACGTGGTGCGCGGCCCGATGCTCGCGCACAAGGCCGAAGACGAGGGCGTGCTGGTGGCCGAGGTGATCGACGG
CCAGAAGCCGCACATCGACTACAACTGCATTCCGTGGGTGATTTACACCTCGCCGGAAATCGCCTGGGTCGGCAAGACCG
AGCAGGCGCTGAAGGCCGAGGGCCGCGAGATCAAGACCGGCAAGTTCCCGTTCTCGATCAACGGCCGCGCGCTCGGCATG
AACGCGCCGGACGGCTTCGTGAAGATGATCGCCGACGCCAAGACCGACGAGGTGCTGGGCGTGCACATCATCTCGGCGAA
TGCCTCGGACCTGATCGCCGAGGCCGTGGTCGCGATGGAATTCAAGGCGGCCTCGGAAGACATCGCCCGCATCTGCCATC
CGCACCCGTCGCTGTCGGAAGTCGTGCGAGAAGCCGCGCTCGCGACCGACAAGCGTTCGATCAACAGCTGA

Upstream 100 bases:

>100_bases
CTCGACCTGTAAGCCTGCGTTTCTCCCGTATTGAATCGCCCCGGGCGCGCGACGCACGCATCGCGTGCGGCCGCGCGCCG
GGCAACAGAGAAGGATAGTC

Downstream 100 bases:

>100_bases
TCGACGCGGCCCCCAGGGCCGCCTGGGCGCCGCGCCGGCTCGCCGGCCGGCGTCGCCATTTGATGCCGAAGGCGGGCGGG
AATTTTCCGCTCGCCTTTGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSKEFDVVVIGAGPGGYVAAIRAAQLGKTVAVVEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENASHHLADHGIS
VGDVKMDTAKMLSRKDGIVEKMTGGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTARNVIIATGSKARH
LPNVPVDNRIVADNEGALNFDTVPNKLAVIGAGVIGLELGSVWRRLGADVTVLEAMPAFLAAADEAVAKEAAKLFKKQGL
DINLGVTIGEVKASDAGVSIAYTDKDGNAKTLEADRLIVSVGRVPNTDNLGLESIGLKANERGFIDVDDHCRTALPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTSPEIAWVGKTEQALKAEGREIKTGKFPFSINGRALGM
NAPDGFVKMIADAKTDEVLGVHIISANASDLIAEAVVAMEFKAASEDIARICHPHPSLSEVVREAALATDKRSINS

Sequences:

>Translated_476_residues
MSKEFDVVVIGAGPGGYVAAIRAAQLGKTVAVVEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENASHHLADHGIS
VGDVKMDTAKMLSRKDGIVEKMTGGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTARNVIIATGSKARH
LPNVPVDNRIVADNEGALNFDTVPNKLAVIGAGVIGLELGSVWRRLGADVTVLEAMPAFLAAADEAVAKEAAKLFKKQGL
DINLGVTIGEVKASDAGVSIAYTDKDGNAKTLEADRLIVSVGRVPNTDNLGLESIGLKANERGFIDVDDHCRTALPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTSPEIAWVGKTEQALKAEGREIKTGKFPFSINGRALGM
NAPDGFVKMIADAKTDEVLGVHIISANASDLIAEAVVAMEFKAASEDIARICHPHPSLSEVVREAALATDKRSINS
>Mature_475_residues
SKEFDVVVIGAGPGGYVAAIRAAQLGKTVAVVEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENASHHLADHGISV
GDVKMDTAKMLSRKDGIVEKMTGGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTARNVIIATGSKARHL
PNVPVDNRIVADNEGALNFDTVPNKLAVIGAGVIGLELGSVWRRLGADVTVLEAMPAFLAAADEAVAKEAAKLFKKQGLD
INLGVTIGEVKASDAGVSIAYTDKDGNAKTLEADRLIVSVGRVPNTDNLGLESIGLKANERGFIDVDDHCRTALPNVYAI
GDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTSPEIAWVGKTEQALKAEGREIKTGKFPFSINGRALGMN
APDGFVKMIADAKTDEVLGVHIISANASDLIAEAVVAMEFKAASEDIARICHPHPSLSEVVREAALATDKRSINS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=47.3903966597077, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=28.7234042553192, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI22035672, Length=474, Percent_Identity=27.8481012658228, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI33519430, Length=467, Percent_Identity=25.6959314775161, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519428, Length=467, Percent_Identity=25.6959314775161, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519426, Length=467, Percent_Identity=25.6959314775161, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277065, Length=467, Percent_Identity=25.6959314775161, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277071, Length=467, Percent_Identity=25.6959314775161, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI291045266, Length=469, Percent_Identity=25.1599147121535, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI291045268, Length=462, Percent_Identity=24.025974025974, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=40.1709401709402, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=31.3684210526316, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1789915, Length=466, Percent_Identity=29.3991416309013, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081717, Length=470, Percent_Identity=27.4468085106383, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=48.2105263157895, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=27.6729559748428, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71982272, Length=481, Percent_Identity=27.027027027027, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=464, Percent_Identity=27.5862068965517, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=464, Percent_Identity=27.5862068965517, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17559934, Length=193, Percent_Identity=31.0880829015544, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=46.2655601659751, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=30.6079664570231, Blast_Score=219, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6325166, Length=476, Percent_Identity=27.1008403361345, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=49.7872340425532, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24640553, Length=490, Percent_Identity=27.5510204081633, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24640549, Length=490, Percent_Identity=27.5510204081633, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24640551, Length=490, Percent_Identity=27.5510204081633, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17737741, Length=490, Percent_Identity=24.6938775510204, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50294; Mature: 50163

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEFDVVVIGAGPGGYVAAIRAAQLGKTVAVVEKWKNPAGALKLGGTCLNVGCIPSKAL
CCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEHHHHCCCCCCEEECCEEEEECCCCCHHH
LASSEEFENASHHLADHGISVGDVKMDTAKMLSRKDGIVEKMTGGIEFLFKKNKITWLKG
HCCCHHHCCHHHHHHHCCCEECCEECHHHHHHHHCCCHHHHHCCCEEEEEECCEEEEEEC
HGKFTGKTDAGVQIEVSGEGETEVVTARNVIIATGSKARHLPNVPVDNRIVADNEGALNF
CCEECCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCEEEECCCCCEEE
DTVPNKLAVIGAGVIGLELGSVWRRLGADVTVLEAMPAFLAAADEAVAKEAAKLFKKQGL
CCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DINLGVTIGEVKASDAGVSIAYTDKDGNAKTLEADRLIVSVGRVPNTDNLGLESIGLKAN
EEEEEEEEEEEECCCCCEEEEEECCCCCCEEEECCEEEEEECCCCCCCCCCCHHCCCCCC
ERGFIDVDDHCRTALPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWV
CCCCEECCCHHHHHCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCEEEE
IYTSPEIAWVGKTEQALKAEGREIKTGKFPFSINGRALGMNAPDGFVKMIADAKTDEVLG
EECCCCEEEECCCHHHHHCCCCEEECCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEE
VHIISANASDLIAEAVVAMEFKAASEDIARICHPHPSLSEVVREAALATDKRSINS
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
SKEFDVVVIGAGPGGYVAAIRAAQLGKTVAVVEKWKNPAGALKLGGTCLNVGCIPSKAL
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEHHHHCCCCCCEEECCEEEEECCCCCHHH
LASSEEFENASHHLADHGISVGDVKMDTAKMLSRKDGIVEKMTGGIEFLFKKNKITWLKG
HCCCHHHCCHHHHHHHCCCEECCEECHHHHHHHHCCCHHHHHCCCEEEEEECCEEEEEEC
HGKFTGKTDAGVQIEVSGEGETEVVTARNVIIATGSKARHLPNVPVDNRIVADNEGALNF
CCEECCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCEEEECCCCCEEE
DTVPNKLAVIGAGVIGLELGSVWRRLGADVTVLEAMPAFLAAADEAVAKEAAKLFKKQGL
CCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DINLGVTIGEVKASDAGVSIAYTDKDGNAKTLEADRLIVSVGRVPNTDNLGLESIGLKAN
EEEEEEEEEEEECCCCCEEEEEECCCCCCEEEECCEEEEEECCCCCCCCCCCHHCCCCCC
ERGFIDVDDHCRTALPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWV
CCCCEECCCHHHHHCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCEEEE
IYTSPEIAWVGKTEQALKAEGREIKTGKFPFSINGRALGMNAPDGFVKMIADAKTDEVLG
EECCCCEEEECCCHHHHHCCCCEEECCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEE
VHIISANASDLIAEAVVAMEFKAASEDIARICHPHPSLSEVVREAALATDKRSINS
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]