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The map label for this gene is sucB [H]

Identifier: 238027575

GI number: 238027575

Start: 2179096

End: 2180367

Strand: Reverse

Name: sucB [H]

Synonym: bglu_1g19930

Alternate gene names: 238027575

Gene position: 2180367-2179096 (Counterclockwise)

Preceding gene: 238027576

Following gene: 238027574

Centisome position: 55.81

GC content: 67.14

Gene sequence:

>1272_bases
ATGGCTATCGTTGAAGTCAAAGTCCCCCAGCTTTCCGAATCGGTATCGGAAGCGACCATGCTGCAGTGGAAGAAGAAGCC
CGGTGAGGCCGTCGCGCAGGACGAAATCCTGATCGAACTCGAAACCGACAAGGTCGTGCTCGAAGTGCCGGCGCCGGCCG
CCGGCGTGCTGTCGCAAGTGCTGCAGAACGACGGCGACACCGTCCTGGCCGATCAGGTGATCGCCACCATCGACACCGAA
GCGAAGGCGGGTGCCGTCGCCGCGGCCGCCGGCAAGGCCGAAGTGCAACCGGCCGCAGCGCCCGTCGCCGTGGCGCCGGC
CGCTCAGCCGGCCGCTGCAGCCGGCTCGAGCCACGCCGCCTCGCCGGCCGCCGCGAAGCTGCTGGCCGAGAAGGGCGTGG
ACGCGAGCCAGGTCGCCGGCACGGGCCGCGACGGCCGCATTACCAAGGGCGACGCGCTGGCCGCGAACGCCGCGCCGGCC
AAGGCCGCTGCCGCACCGGCCGCCGCCGCGCCGAAGAAGGCCGCGCTGCCGGACGTGAAGGTGCCGGCCTCGGCCACCAC
CTGGCTGAACGACCGTCCGGAGCAGCGCGTGCCGATGTCGCGCCTGCGCGCGCGGATCGCCGAGCGTCTGCTCGAATCGC
AGCAGACCAACGCGATCCTGACGACGTTCAACGAAGTGAACATGCAGCCCGTCATGGACCTGCGCGCGAAGTACAAGGAC
AAGTTCGAGAAGGAACACGGCGTGAAGCTCGGCTTCATGTCGTTCTTCGTCAAGGCGGCGGTCCACGCGCTGAAGAAGTT
CCCGCTCGTGAACGCGTCGATCGACGGCAACGACATCGTCTACCACGGCTATTTCGACATCGGCATCGCGGTCGGTTCGC
CGCGCGGCCTGGTGGTGCCGATCCTGCGCAACGCCGACCAATTGAGCCTCGCCGACATCGAGAAGAAGATCGCCGAATTC
GGCCAGAAGGCCAAGGACGGCAAGCTGTCGATCGAGGAAATGACGGGCGGCACGTTCTCGATCTCGAACGGCGGCGTGTT
CGGCTCGATGCTCTCGACCCCGATCATCAACCCGCCGCAGTCGGCGATCCTCGGCGTTCACGCGACCAAGGAGCGTCCGG
TGGTCGAGAACGGCCAGATCGTGATCCGCCCGATCAACTACCTCGCGCTGTCCTATGACCACCGGATCATCGACGGTCGC
GAAGCGGTGCTGTCGCTGGTGGCGATGAAGGATGCGCTGGAAGATCCGGCGCGCCTGCTGCTCGACCTGTAA

Upstream 100 bases:

>100_bases
GCGGCAAGCGCGCCCGGGCCGTCTTCGCGCGTGCCGCGTAGTCGCGCGCCCGTCCGCAGAACCCCGACATTACCCAGATC
AAGCATCCAGGAATCAAGTC

Downstream 100 bases:

>100_bases
GCCTGCGTTTCTCCCGTATTGAATCGCCCCGGGCGCGCGACGCACGCATCGCGTGCGGCCGCGCGCCGGGCAACAGAGAA
GGATAGTCATGTCCAAGGAA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLSQVLQNDGDTVLADQVIATIDTE
AKAGAVAAAAGKAEVQPAAAPVAVAPAAQPAAAAGSSHAASPAAAKLLAEKGVDASQVAGTGRDGRITKGDALAANAAPA
KAAAAPAAAAPKKAALPDVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKD
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLADIEKKIAEF
GQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGR
EAVLSLVAMKDALEDPARLLLDL

Sequences:

>Translated_423_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLSQVLQNDGDTVLADQVIATIDTE
AKAGAVAAAAGKAEVQPAAAPVAVAPAAQPAAAAGSSHAASPAAAKLLAEKGVDASQVAGTGRDGRITKGDALAANAAPA
KAAAAPAAAAPKKAALPDVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKD
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLADIEKKIAEF
GQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGR
EAVLSLVAMKDALEDPARLLLDL
>Mature_422_residues
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLSQVLQNDGDTVLADQVIATIDTEA
KAGAVAAAAGKAEVQPAAAPVAVAPAAQPAAAAGSSHAASPAAAKLLAEKGVDASQVAGTGRDGRITKGDALAANAAPAK
AAAAPAAAAPKKAALPDVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKDK
FEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLADIEKKIAEFG
QKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGRE
AVLSLVAMKDALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=56.8376068376068, Blast_Score=287, Evalue=2e-77,
Organism=Homo sapiens, GI31711992, Length=446, Percent_Identity=31.8385650224215, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=28.7671232876712, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI203098753, Length=458, Percent_Identity=29.9126637554585, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=29.7826086956522, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.2670807453416, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=54.6099290780142, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=31.9347319347319, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=422, Percent_Identity=44.3127962085308, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI17560088, Length=451, Percent_Identity=31.4855875831486, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=26.0969976905312, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=32.5949367088608, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=43.3014354066986, Blast_Score=333, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=30.1762114537445, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645909, Length=241, Percent_Identity=56.0165975103734, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI18859875, Length=437, Percent_Identity=28.1464530892449, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=34.2105263157895, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=34.2105263157895, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44335; Mature: 44204

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLSQV
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVLADQVIATIDTEAKAGAVAAAAGKAEVQPAAAPVAVAPAAQPAAAAGSSHAA
HHCCCCEEEHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCHHHCCCCCCC
SPAAAKLLAEKGVDASQVAGTGRDGRITKGDALAANAAPAKAAAAPAAAAPKKAALPDVK
CHHHHHHHHHCCCCHHHHCCCCCCCCEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
VPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHHHH
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVP
HHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEE
ILRNADQLSLADIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQ
EECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCCCH
SAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPARLL
HHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHH
LDL
HCC
>Mature Secondary Structure 
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLSQV
EEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVLADQVIATIDTEAKAGAVAAAAGKAEVQPAAAPVAVAPAAQPAAAAGSSHAA
HHCCCCEEEHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCHHHCCCCCCC
SPAAAKLLAEKGVDASQVAGTGRDGRITKGDALAANAAPAKAAAAPAAAAPKKAALPDVK
CHHHHHHHHHCCCCHHHHCCCCCCCCEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
VPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHHHH
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVP
HHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEE
ILRNADQLSLADIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQ
EECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCCCH
SAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPARLL
HHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHH
LDL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]