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The map label for this gene is lcfB [H]

Identifier: 238027493

GI number: 238027493

Start: 2085745

End: 2087448

Strand: Reverse

Name: lcfB [H]

Synonym: bglu_1g19060

Alternate gene names: 238027493

Gene position: 2087448-2085745 (Counterclockwise)

Preceding gene: 238027494

Following gene: 238027492

Centisome position: 53.44

GC content: 58.57

Gene sequence:

>1704_bases
ATGCCGAATGGGCGACCGATGACGCAGACAGCGAGTAAAACAACTATGAACCGCGACCCCTGGTGTTATCGGGGAAAGCG
GCTCACCGAGCGGATTATCCAGCTAACCACATTGCCGACGGTGATCGATCGACATGCTGCCGGCGAGGGGATGGATCGGC
CTGCGTTGACTTGGTATGTTGATCAAGCGCGAACTACACATTGGACGTACCGGGAGATGATCGCGGACATTCATTGTCTT
GCTGCAGAGTTGGCTCTACATCATCGCGTGTCGCGAGGTGATCGCGTGATGGTCATTTCGGCCAACTGCCCCGAAGCCTA
CCTTACTTATCTGGCGATTCTTTATATCGGCGCTATTGCGGTGCCCGTCAATAATGTCGAGTCGACGCGCAATCTGCAAT
ACATTGCCGGACAAGTCCAGCCTGTATTGACGGTCACCGGCCTGGGCGTCGGCGCCGATTTGCTGGTGGCGATCGATGCA
TCCAGGATAGGGACTGAGGAACTATTAGCGAATGCGAGGGCACGGGGGGCTGCGAGTATGCCGTCTCCGGCCGTCGAGCC
CGACGATCTTGCGGTGCTGTTGTTTACCTCAGGCACGACTTCATCACCCAAGGGCGTCCGCCTGAGCCATTACAACATCT
TGGTCAATGCCGAGGGATTGAGGATCGCCCACGCTCTCGACGTGAATCAACATCATCTTTGCATCCTACCGTTGTTTCAC
GCGAATGCCTTTGGTTTCTCGATGATTGGTTCGGTTTACGCAGGATGTCACACGGTTTTATGCAACCGCTTCTCAGGCCA
GGGGCTTTGGGAGATCGCCAGCCGCGAGCAAATCAACATCTTGTCGGCAGTTCCGGAAATCATTCGTTTGCTCGGCAGCC
GCCGGTCGAAGCCGGAGCTGTCGCCTGATTTCCGCTATGTCGTGTCCGCTGCCGCACCCCTGTCGCGCACCGACGCCGCA
TCGTTCGAAAAAAACCTCGGTATTCCCATACACCAGGGTTACGGTCTCAGCGAATGCACGAATTTCGCCGCTACCACGCC
GGCGTCGATCGATCCGATCGTTCGCAGGCGTTTGATGAATGAGTGGAACGTACCGAGCATCGGCCCGGCGTTGTTTGGCA
CCGAACTGAAGATCGCGCGGCGGGATGGTTCGCCTGCAGACCAGGGCGAGGAGGGCGAGATTCTGATTCGCGGCCACAAC
GTGATGTCCGGCTATTGGCGGGCCGACGAAGAAACCTCTCTGGCATTCGCCGACGGATGGTTGCACAGCGGCGATCTCGG
CTTCTTCGTCCAGGTGGAGGAGGTGCCGTATTTTTTCGTCACGGGCCGCCTCAAGGAGATCATCCTCCGGCGCGGCGACA
GCATCAGCCCCCGAGCAGTGGAGGCGGAATTGGCTGCTCTGTCGCATGTCGGCCGCTTCGCCGCTTGCGGTTTTCGTAAC
GAATGGGCCGGGGAAGAAATTGGCGTCTATGTCTTTACAGACGACATCGACCATGCGTTGGCCGAGGTGCGAGCGATCGT
GGAGCGCTGCTCGCCGCGGTATCGTCCTGGCGTTGTGATGGTCGGTGCGCGCACGATACCGGCCACGGTGACAGGAAAGA
TTCAGCGTAGGCAACTTGCTTCGCGGTTTGGTGTTTTTGCTGCCGCAGCGTTGGGTGGAAAGCCCCGTGTGGTGGCCGAC
GAAGCGACCGGTGGTAACGGATGA

Upstream 100 bases:

>100_bases
GGGCACGGAAATCCCCGGGCTCGACCCCGCGTGGAAAAAAGTGATGACCGACGACGCATGGTAGTACGAAGGCCAACGCG
GTCGATTGATCAACCAGTAA

Downstream 100 bases:

>100_bases
GCGCGATCATCGCTTATTCGGATCGTTGGAGCGTGGCGCCGGGTGAGACGATTCGCTTCATGGTGAGCAGCGCGAATGCT
GAACCGTATGATGTACAAAT

Product: acyl-CoA-ligase/synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MPNGRPMTQTASKTTMNRDPWCYRGKRLTERIIQLTTLPTVIDRHAAGEGMDRPALTWYVDQARTTHWTYREMIADIHCL
AAELALHHRVSRGDRVMVISANCPEAYLTYLAILYIGAIAVPVNNVESTRNLQYIAGQVQPVLTVTGLGVGADLLVAIDA
SRIGTEELLANARARGAASMPSPAVEPDDLAVLLFTSGTTSSPKGVRLSHYNILVNAEGLRIAHALDVNQHHLCILPLFH
ANAFGFSMIGSVYAGCHTVLCNRFSGQGLWEIASREQINILSAVPEIIRLLGSRRSKPELSPDFRYVVSAAAPLSRTDAA
SFEKNLGIPIHQGYGLSECTNFAATTPASIDPIVRRRLMNEWNVPSIGPALFGTELKIARRDGSPADQGEEGEILIRGHN
VMSGYWRADEETSLAFADGWLHSGDLGFFVQVEEVPYFFVTGRLKEIILRRGDSISPRAVEAELAALSHVGRFAACGFRN
EWAGEEIGVYVFTDDIDHALAEVRAIVERCSPRYRPGVVMVGARTIPATVTGKIQRRQLASRFGVFAAAALGGKPRVVAD
EATGGNG

Sequences:

>Translated_567_residues
MPNGRPMTQTASKTTMNRDPWCYRGKRLTERIIQLTTLPTVIDRHAAGEGMDRPALTWYVDQARTTHWTYREMIADIHCL
AAELALHHRVSRGDRVMVISANCPEAYLTYLAILYIGAIAVPVNNVESTRNLQYIAGQVQPVLTVTGLGVGADLLVAIDA
SRIGTEELLANARARGAASMPSPAVEPDDLAVLLFTSGTTSSPKGVRLSHYNILVNAEGLRIAHALDVNQHHLCILPLFH
ANAFGFSMIGSVYAGCHTVLCNRFSGQGLWEIASREQINILSAVPEIIRLLGSRRSKPELSPDFRYVVSAAAPLSRTDAA
SFEKNLGIPIHQGYGLSECTNFAATTPASIDPIVRRRLMNEWNVPSIGPALFGTELKIARRDGSPADQGEEGEILIRGHN
VMSGYWRADEETSLAFADGWLHSGDLGFFVQVEEVPYFFVTGRLKEIILRRGDSISPRAVEAELAALSHVGRFAACGFRN
EWAGEEIGVYVFTDDIDHALAEVRAIVERCSPRYRPGVVMVGARTIPATVTGKIQRRQLASRFGVFAAAALGGKPRVVAD
EATGGNG
>Mature_566_residues
PNGRPMTQTASKTTMNRDPWCYRGKRLTERIIQLTTLPTVIDRHAAGEGMDRPALTWYVDQARTTHWTYREMIADIHCLA
AELALHHRVSRGDRVMVISANCPEAYLTYLAILYIGAIAVPVNNVESTRNLQYIAGQVQPVLTVTGLGVGADLLVAIDAS
RIGTEELLANARARGAASMPSPAVEPDDLAVLLFTSGTTSSPKGVRLSHYNILVNAEGLRIAHALDVNQHHLCILPLFHA
NAFGFSMIGSVYAGCHTVLCNRFSGQGLWEIASREQINILSAVPEIIRLLGSRRSKPELSPDFRYVVSAAAPLSRTDAAS
FEKNLGIPIHQGYGLSECTNFAATTPASIDPIVRRRLMNEWNVPSIGPALFGTELKIARRDGSPADQGEEGEILIRGHNV
MSGYWRADEETSLAFADGWLHSGDLGFFVQVEEVPYFFVTGRLKEIILRRGDSISPRAVEAELAALSHVGRFAACGFRNE
WAGEEIGVYVFTDDIDHALAEVRAIVERCSPRYRPGVVMVGARTIPATVTGKIQRRQLASRFGVFAAAALGGKPRVVADE
ATGGNG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=514, Percent_Identity=25.6809338521401, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI187761343, Length=514, Percent_Identity=25.6809338521401, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI156151445, Length=546, Percent_Identity=24.1758241758242, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI57165410, Length=325, Percent_Identity=27.6923076923077, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI57165412, Length=325, Percent_Identity=27.6923076923077, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI115511026, Length=531, Percent_Identity=20.9039548022599, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI122937307, Length=531, Percent_Identity=21.2806026365348, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI83745141, Length=159, Percent_Identity=35.2201257861635, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI42794760, Length=160, Percent_Identity=30, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI42794758, Length=160, Percent_Identity=30, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI42794756, Length=160, Percent_Identity=30, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI221142682, Length=496, Percent_Identity=25, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI145693145, Length=497, Percent_Identity=28.3702213279678, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=23.9130434782609, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1786810, Length=525, Percent_Identity=24.3809523809524, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1789201, Length=323, Percent_Identity=25.077399380805, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790505, Length=554, Percent_Identity=22.7436823104693, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=430, Percent_Identity=27.6744186046512, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI32563687, Length=424, Percent_Identity=26.1792452830189, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=377, Percent_Identity=29.4429708222812, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=389, Percent_Identity=26.7352185089974, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=372, Percent_Identity=26.8817204301075, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=418, Percent_Identity=27.0334928229665, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=365, Percent_Identity=25.4794520547945, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=475, Percent_Identity=22.3157894736842, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=24.015009380863, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=24.015009380863, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=24.015009380863, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=23.3009708737864, Blast_Score=99, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=24.5125348189415, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859661, Length=513, Percent_Identity=28.8499025341131, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355181, Length=480, Percent_Identity=27.7083333333333, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24581924, Length=392, Percent_Identity=26.7857142857143, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653035, Length=387, Percent_Identity=25.5813953488372, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=363, Percent_Identity=24.2424242424242, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656500, Length=491, Percent_Identity=23.6252545824847, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356947, Length=397, Percent_Identity=25.4408060453401, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=296, Percent_Identity=25.3378378378378, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922652, Length=493, Percent_Identity=23.9350912778905, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61748; Mature: 61617

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNGRPMTQTASKTTMNRDPWCYRGKRLTERIIQLTTLPTVIDRHAAGEGMDRPALTWYV
CCCCCCCCCCHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DQARTTHWTYREMIADIHCLAAELALHHRVSRGDRVMVISANCPEAYLTYLAILYIGAIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHE
VPVNNVESTRNLQYIAGQVQPVLTVTGLGVGADLLVAIDASRIGTEELLANARARGAASM
EECCCCCCCCCHHHHHCCCCEEEEEECCCCCCEEEEEEECHHCCHHHHHHHHHCCCCCCC
PSPAVEPDDLAVLLFTSGTTSSPKGVRLSHYNILVNAEGLRIAHALDVNQHHLCILPLFH
CCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEEECCCEEEEEEECCCCCCEEEEEEEC
ANAFGFSMIGSVYAGCHTVLCNRFSGQGLWEIASREQINILSAVPEIIRLLGSRRSKPEL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
SPDFRYVVSAAAPLSRTDAASFEKNLGIPIHQGYGLSECTNFAATTPASIDPIVRRRLMN
CCCHHHHHHHCCCCCCCCHHHHHHCCCCEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHC
EWNVPSIGPALFGTELKIARRDGSPADQGEEGEILIRGHNVMSGYWRADEETSLAFADGW
CCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCEEECEECCCCCCEEEEECCC
LHSGDLGFFVQVEEVPYFFVTGRLKEIILRRGDSISPRAVEAELAALSHVGRFAACGFRN
EECCCEEEEEEEECCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
EWAGEEIGVYVFTDDIDHALAEVRAIVERCSPRYRPGVVMVGARTIPATVTGKIQRRQLA
CCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECEECCCHHCCHHHHHHHH
SRFGVFAAAALGGKPRVVADEATGGNG
HHHHHHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
PNGRPMTQTASKTTMNRDPWCYRGKRLTERIIQLTTLPTVIDRHAAGEGMDRPALTWYV
CCCCCCCCCHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DQARTTHWTYREMIADIHCLAAELALHHRVSRGDRVMVISANCPEAYLTYLAILYIGAIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHE
VPVNNVESTRNLQYIAGQVQPVLTVTGLGVGADLLVAIDASRIGTEELLANARARGAASM
EECCCCCCCCCHHHHHCCCCEEEEEECCCCCCEEEEEEECHHCCHHHHHHHHHCCCCCCC
PSPAVEPDDLAVLLFTSGTTSSPKGVRLSHYNILVNAEGLRIAHALDVNQHHLCILPLFH
CCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEEECCCEEEEEEECCCCCCEEEEEEEC
ANAFGFSMIGSVYAGCHTVLCNRFSGQGLWEIASREQINILSAVPEIIRLLGSRRSKPEL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
SPDFRYVVSAAAPLSRTDAASFEKNLGIPIHQGYGLSECTNFAATTPASIDPIVRRRLMN
CCCHHHHHHHCCCCCCCCHHHHHHCCCCEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHC
EWNVPSIGPALFGTELKIARRDGSPADQGEEGEILIRGHNVMSGYWRADEETSLAFADGW
CCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCEEECEECCCCCCEEEEECCC
LHSGDLGFFVQVEEVPYFFVTGRLKEIILRRGDSISPRAVEAELAALSHVGRFAACGFRN
EECCCEEEEEEEECCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
EWAGEEIGVYVFTDDIDHALAEVRAIVERCSPRYRPGVVMVGARTIPATVTGKIQRRQLA
CCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECEECCCHHCCHHHHHHHH
SRFGVFAAAALGGKPRVVADEATGGNG
HHHHHHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]