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The map label for this gene is rutF [H]

Identifier: 238025303

GI number: 238025303

Start: 2457673

End: 2458197

Strand: Reverse

Name: rutF [H]

Synonym: bglu_2g19770

Alternate gene names: 238025303

Gene position: 2458197-2457673 (Counterclockwise)

Preceding gene: 339717981

Following gene: 238025302

Centisome position: 86.94

GC content: 72.57

Gene sequence:

>525_bases
ATGATCCACAACGAACCCGCGCGGTTTCCCGATTCGCCGCTCGCGGCATCGCCGCAGCAGTTCCGAGACGCGATGTGCCG
CGTGGCGTCGGCCGTTCACGTGCTCACCAGCGACGGGCCGGCCGGCCGCTGCGGCATGACGGCCACCGCCGTACTGTCGG
TGTCCGACGCACCGCCGACGGTGCTCGTGTCGGTAAACCGCGGCAGCGTGTCGGCGCAGCGGCTGATCGCGAACGAGGTG
TTCTGCGTGAACACGCTCGGCGCGGCCGACCGCTCGATCGCCGAGGTGTTCGCGGGCCGCACCGCGCACGAGCGCGACGC
ACGCTTCACGGCGGGCGAGTGGGGCGAGCTCGCGACGCGCGCACCGGTGCTGTCGAGCGCCCGCGTCGCGCTCGACTGCC
GGCTCGTGGCAACGCTGAGCGCGGCGACGCACGAGCTGCTGGTGGGCGAAGTGGTGGCGCTGCGCTGCGCGGCGCCCGAC
GACGAGGCGCTCGTCTATCTGCAGCGCGAATTCCGCGCCGTATGA

Upstream 100 bases:

>100_bases
TGCATGACGCTGGGCGGCCTGCTCGCGCTGTCGCTGCCGAAGACGCTGGTCAACCGCTAGCGCGACGCGCGGCCTTTTCT
CTCATGCACGGAGACAGGAC

Downstream 100 bases:

>100_bases
CGGCGCGCGCCGACCGGACGATCGATATGAAACGCGAAATCGTACAAGCCACCAACCGCTGCCGCAGCGAGATCCTCGCC
GGCGGTTTTCGTAGCGACGT

Product: 4-hydroxyphenylacetate 3-monooxygenase reductase subunit

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MIHNEPARFPDSPLAASPQQFRDAMCRVASAVHVLTSDGPAGRCGMTATAVLSVSDAPPTVLVSVNRGSVSAQRLIANEV
FCVNTLGAADRSIAEVFAGRTAHERDARFTAGEWGELATRAPVLSSARVALDCRLVATLSAATHELLVGEVVALRCAAPD
DEALVYLQREFRAV

Sequences:

>Translated_174_residues
MIHNEPARFPDSPLAASPQQFRDAMCRVASAVHVLTSDGPAGRCGMTATAVLSVSDAPPTVLVSVNRGSVSAQRLIANEV
FCVNTLGAADRSIAEVFAGRTAHERDARFTAGEWGELATRAPVLSSARVALDCRLVATLSAATHELLVGEVVALRCAAPD
DEALVYLQREFRAV
>Mature_174_residues
MIHNEPARFPDSPLAASPQQFRDAMCRVASAVHVLTSDGPAGRCGMTATAVLSVSDAPPTVLVSVNRGSVSAQRLIANEV
FCVNTLGAADRSIAEVFAGRTAHERDARFTAGEWGELATRAPVLSSARVALDCRLVATLSAATHELLVGEVVALRCAAPD
DEALVYLQREFRAV

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=157, Percent_Identity=41.4012738853503, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18370; Mature: 18370

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHNEPARFPDSPLAASPQQFRDAMCRVASAVHVLTSDGPAGRCGMTATAVLSVSDAPPT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEEECCCCCE
VLVSVNRGSVSAQRLIANEVFCVNTLGAADRSIAEVFAGRTAHERDARFTAGEWGELATR
EEEEECCCCCHHHHHHHHCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHC
APVLSSARVALDCRLVATLSAATHELLVGEVVALRCAAPDDEALVYLQREFRAV
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCHHEEEEEHHHHCC
>Mature Secondary Structure
MIHNEPARFPDSPLAASPQQFRDAMCRVASAVHVLTSDGPAGRCGMTATAVLSVSDAPPT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEEECCCCCE
VLVSVNRGSVSAQRLIANEVFCVNTLGAADRSIAEVFAGRTAHERDARFTAGEWGELATR
EEEEECCCCCHHHHHHHHCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHC
APVLSSARVALDCRLVATLSAATHELLVGEVVALRCAAPDDEALVYLQREFRAV
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCHHEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA