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The map label for this gene is hipO [H]

Identifier: 238025223

GI number: 238025223

Start: 2363171

End: 2364370

Strand: Reverse

Name: hipO [H]

Synonym: bglu_2g18940

Alternate gene names: 238025223

Gene position: 2364370-2363171 (Counterclockwise)

Preceding gene: 238025224

Following gene: 238025221

Centisome position: 83.63

GC content: 71.67

Gene sequence:

>1200_bases
ATGAAACTGATCGAATCCATCCAGGCCGACGCGGCCGAACTGCAGGCGATCCGCCGCGACATCCACGCCCATCCCGAGAT
CGGCTATGACGTGTTGCGCACCGCCGAACTCGTCGCCGCACGGCTCGAGGGCTGGGGCTACCTGGTCACGCGCGGCGTCG
GTCGCAGCGGCGTGGTGGGCACGCTGCGGCGCGGCACCAGCCCGCGCGCGATCGGCTTGCGCGCCGACATGGACGCGCTG
CCGGTGCAGGAGGCCAACGACTTCGCGCACCGCTCGACGGTGGCCGGCGCGATGCACGCATGCGGCCACGACGGCCACAC
CGCGATGCTGCTCGGTGCGGCCCGCCATCTGGCGCGCGAGGGCGAGTTCGACGGCACCGTGCAGTTGTTCTTCCAGCCGG
CCGAGGAGGCGGGCGGCGGCGCGCGCGCGATGATCGAGGATGGCCTGTTCGCGCGCTTTCCGGTGGATGCCGTGTTCGGG
CTGCACAACTGGCCCGGCATCGCGGCCGGCGATTTCGCGGTGCGCCCCGGGCCGCTGATGGCCTCCACCAGCCTGTTCCG
CATCACGCTGCGCGGTGCCGGCTGCCACGCGGCGATGCCGCATCTGGGCCGCGACCCGGTGTTCGCGGCCGGGCAGGTGC
TGAGCGCGCTGCAGGGCATCGTCACGCGCAATCGCAATCCGATCGAAGGCGCGGTGCTGTCGGTCACGCAGATCCATGCC
GGCGAGGCGATGAACGTGGTGCCCACCGATGCATGGCTAGGCGGCACCGTGCGGACCTTCTCCGATGCCACGCTCGGGCT
GATCGAGCGGCGCATGCGCGCGGTGGTGGCCGCTACCGCGGCGGCGTTCGAGTGCGAGAGCGAGGTGGAGTTCACGCACC
AGTATCCGGCCACCGTCAACGACGCCGCGCAGACCGCCTTCGCGGCCGGCGTGATGCGCGAGCTGGTGGGCGACGAGCAC
GTCGACGCGGCGGCCGAGCCGACCATGGCCGCCGAGGATTTCTCGTTCATGCTGCGCGAGCGGCCCGGCTGCTATGCGTT
CATCGGCAACGGCACGGGCGAGCACCGCGCGATGGGGCATGGCGGCGGGCCTTGCCTGCTGCACAACGCCAGCTACGACT
TCAACGACGCGCTGCTGCCGGTGGGCGCCAGCTATTTCGTGCGGCTGGTGGAGCGGTTCCTGGCGCCGGTGCCGGGCTGA

Upstream 100 bases:

>100_bases
GTCGTTCGTCGCGAACGCCGGCACCTTCCTCGGTGCCACGCTGGTCGCGCAGCTGTTCCACATCTGAGCGGCCGCCCGGC
AAGACCTCAAGGACTCCCGC

Downstream 100 bases:

>100_bases
GCGGCCCGCGCGGGGCGCGGGCATGCCGCCTCGCCTCGCGCCTACAAGGAAGGCGCCCAGGTGGTGCCGAACGGATTCGA
CTGATCGATGCCCGGGAAGT

Product: Amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKLIESIQADAAELQAIRRDIHAHPEIGYDVLRTAELVAARLEGWGYLVTRGVGRSGVVGTLRRGTSPRAIGLRADMDAL
PVQEANDFAHRSTVAGAMHACGHDGHTAMLLGAARHLAREGEFDGTVQLFFQPAEEAGGGARAMIEDGLFARFPVDAVFG
LHNWPGIAAGDFAVRPGPLMASTSLFRITLRGAGCHAAMPHLGRDPVFAAGQVLSALQGIVTRNRNPIEGAVLSVTQIHA
GEAMNVVPTDAWLGGTVRTFSDATLGLIERRMRAVVAATAAAFECESEVEFTHQYPATVNDAAQTAFAAGVMRELVGDEH
VDAAAEPTMAAEDFSFMLRERPGCYAFIGNGTGEHRAMGHGGGPCLLHNASYDFNDALLPVGASYFVRLVERFLAPVPG

Sequences:

>Translated_399_residues
MKLIESIQADAAELQAIRRDIHAHPEIGYDVLRTAELVAARLEGWGYLVTRGVGRSGVVGTLRRGTSPRAIGLRADMDAL
PVQEANDFAHRSTVAGAMHACGHDGHTAMLLGAARHLAREGEFDGTVQLFFQPAEEAGGGARAMIEDGLFARFPVDAVFG
LHNWPGIAAGDFAVRPGPLMASTSLFRITLRGAGCHAAMPHLGRDPVFAAGQVLSALQGIVTRNRNPIEGAVLSVTQIHA
GEAMNVVPTDAWLGGTVRTFSDATLGLIERRMRAVVAATAAAFECESEVEFTHQYPATVNDAAQTAFAAGVMRELVGDEH
VDAAAEPTMAAEDFSFMLRERPGCYAFIGNGTGEHRAMGHGGGPCLLHNASYDFNDALLPVGASYFVRLVERFLAPVPG
>Mature_399_residues
MKLIESIQADAAELQAIRRDIHAHPEIGYDVLRTAELVAARLEGWGYLVTRGVGRSGVVGTLRRGTSPRAIGLRADMDAL
PVQEANDFAHRSTVAGAMHACGHDGHTAMLLGAARHLAREGEFDGTVQLFFQPAEEAGGGARAMIEDGLFARFPVDAVFG
LHNWPGIAAGDFAVRPGPLMASTSLFRITLRGAGCHAAMPHLGRDPVFAAGQVLSALQGIVTRNRNPIEGAVLSVTQIHA
GEAMNVVPTDAWLGGTVRTFSDATLGLIERRMRAVVAATAAAFECESEVEFTHQYPATVNDAAQTAFAAGVMRELVGDEH
VDAAAEPTMAAEDFSFMLRERPGCYAFIGNGTGEHRAMGHGGGPCLLHNASYDFNDALLPVGASYFVRLVERFLAPVPG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=444, Percent_Identity=28.3783783783784, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787598, Length=213, Percent_Identity=31.924882629108, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42332; Mature: 42332

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIESIQADAAELQAIRRDIHAHPEIGYDVLRTAELVAARLEGWGYLVTRGVGRSGVVG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
TLRRGTSPRAIGLRADMDALPVQEANDFAHRSTVAGAMHACGHDGHTAMLLGAARHLARE
HHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GEFDGTVQLFFQPAEEAGGGARAMIEDGLFARFPVDAVFGLHNWPGIAAGDFAVRPGPLM
CCCCCEEEEEEEEHHHCCCCEEEHHHCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCE
ASTSLFRITLRGAGCHAAMPHLGRDPVFAAGQVLSALQGIVTRNRNPIEGAVLSVTQIHA
ECCEEEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECC
GEAMNVVPTDAWLGGTVRTFSDATLGLIERRMRAVVAATAAAFECESEVEFTHQYPATVN
CCCCEEECCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
DAAQTAFAAGVMRELVGDEHVDAAAEPTMAAEDFSFMLRERPGCYAFIGNGTGEHRAMGH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
GGGPCLLHNASYDFNDALLPVGASYFVRLVERFLAPVPG
CCCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLIESIQADAAELQAIRRDIHAHPEIGYDVLRTAELVAARLEGWGYLVTRGVGRSGVVG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
TLRRGTSPRAIGLRADMDALPVQEANDFAHRSTVAGAMHACGHDGHTAMLLGAARHLARE
HHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GEFDGTVQLFFQPAEEAGGGARAMIEDGLFARFPVDAVFGLHNWPGIAAGDFAVRPGPLM
CCCCCEEEEEEEEHHHCCCCEEEHHHCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCE
ASTSLFRITLRGAGCHAAMPHLGRDPVFAAGQVLSALQGIVTRNRNPIEGAVLSVTQIHA
ECCEEEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECC
GEAMNVVPTDAWLGGTVRTFSDATLGLIERRMRAVVAATAAAFECESEVEFTHQYPATVN
CCCCEEECCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
DAAQTAFAAGVMRELVGDEHVDAAAEPTMAAEDFSFMLRERPGCYAFIGNGTGEHRAMGH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
GGGPCLLHNASYDFNDALLPVGASYFVRLVERFLAPVPG
CCCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]