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The map label for this gene is maiA [H]

Identifier: 238025189

GI number: 238025189

Start: 2318149

End: 2318817

Strand: Reverse

Name: maiA [H]

Synonym: bglu_2g18600

Alternate gene names: 238025189

Gene position: 2318817-2318149 (Counterclockwise)

Preceding gene: 238025190

Following gene: 238025188

Centisome position: 82.01

GC content: 71.75

Gene sequence:

>669_bases
ATGACTGAGGCCACGCTCTACGGCTATTTCCGCAGCTCGGCGTCGTACCGCGTGCGCATCGCGCTGAACCTGAAGGGCAT
CGACTACCGGCAGATTCCCGTGCATCTGCTGCGCGGCGGCGGCGAGCAGCTCGGCGAGGCGTACCGGCAGATCCAGCCCG
ACGGCGTGGTGCCCGCCTTCGTCGACGGCGACGGGCCGCCGCTCGCGCAGTCGCTCGCGATCGTCGAATACCTCGACGAG
TGCTTTCCGCAGCCGCCGCTGCTGCCCTCGGCTCCGGCCGATCGCGCCTACGTGCGCGCGGTCGCGCTGCAGATCGCCTG
CGAGATCCATCCGCTCAACAACCTGCGCGTGCTGAGCTACCTGAAGGCCACGCTCGGCGTCAGCGACGCGCAGAAAAGCG
CCTGGTACGCGCACTGGATCGGGCTCGGCTTCGCCTCGCTCGAACGCCGGCTCGCCGCCGAGCCGCGCGTGGGCCGCTGC
GTGTTCGGCGACACGCCGGGGCTGGCCGATCTCTGCCTGGTGCCGCAGGTGTGGAATGCGAAACGCTTCGCGGTGCCGCT
CGATGCCTACCCCACGCTCATGCGCCTGTACGGGCACGCGGCGGCGCTGCCGGCCTTCGCGCGCGCCGAGCCGTCGCTGC
AGCCCGACGCGGAGCCGCCGCCCGCCTAG

Upstream 100 bases:

>100_bases
CGCGGCCACTGCCGCGGCGACGGCTATCGCATCGGCTTCGGCAGCTGTGTCGGCACCATCCTGCCGGCACTGGCCGATAC
CGCGGAAGACGGGGCGCGCG

Downstream 100 bases:

>100_bases
CGCGTCTTGCCGGCAGCCTGCCGGCGCCGCGATTGGCATGATCGCGGTTCGTCCGAGTCGGCCCGGGCCGGCCAGATCCG
GTTCGACTCGCCCGCCGGCG

Product: Maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTEATLYGYFRSSASYRVRIALNLKGIDYRQIPVHLLRGGGEQLGEAYRQIQPDGVVPAFVDGDGPPLAQSLAIVEYLDE
CFPQPPLLPSAPADRAYVRAVALQIACEIHPLNNLRVLSYLKATLGVSDAQKSAWYAHWIGLGFASLERRLAAEPRVGRC
VFGDTPGLADLCLVPQVWNAKRFAVPLDAYPTLMRLYGHAAALPAFARAEPSLQPDAEPPPA

Sequences:

>Translated_222_residues
MTEATLYGYFRSSASYRVRIALNLKGIDYRQIPVHLLRGGGEQLGEAYRQIQPDGVVPAFVDGDGPPLAQSLAIVEYLDE
CFPQPPLLPSAPADRAYVRAVALQIACEIHPLNNLRVLSYLKATLGVSDAQKSAWYAHWIGLGFASLERRLAAEPRVGRC
VFGDTPGLADLCLVPQVWNAKRFAVPLDAYPTLMRLYGHAAALPAFARAEPSLQPDAEPPPA
>Mature_221_residues
TEATLYGYFRSSASYRVRIALNLKGIDYRQIPVHLLRGGGEQLGEAYRQIQPDGVVPAFVDGDGPPLAQSLAIVEYLDEC
FPQPPLLPSAPADRAYVRAVALQIACEIHPLNNLRVLSYLKATLGVSDAQKSAWYAHWIGLGFASLERRLAAEPRVGRCV
FGDTPGLADLCLVPQVWNAKRFAVPLDAYPTLMRLYGHAAALPAFARAEPSLQPDAEPPPA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=212, Percent_Identity=44.811320754717, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI22202622, Length=154, Percent_Identity=42.8571428571429, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=34.4339622641509, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17551302, Length=213, Percent_Identity=40.3755868544601, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17510461, Length=218, Percent_Identity=38.0733944954128, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17556142, Length=214, Percent_Identity=39.2523364485981, Blast_Score=123, Evalue=9e-29,
Organism=Drosophila melanogaster, GI45553325, Length=219, Percent_Identity=44.2922374429224, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21355859, Length=214, Percent_Identity=44.8598130841121, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24645375, Length=214, Percent_Identity=44.8598130841121, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21355857, Length=219, Percent_Identity=40.6392694063927, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24193; Mature: 24062

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEATLYGYFRSSASYRVRIALNLKGIDYRQIPVHLLRGGGEQLGEAYRQIQPDGVVPAF
CCCCEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEE
VDGDGPPLAQSLAIVEYLDECFPQPPLLPSAPADRAYVRAVALQIACEIHPLNNLRVLSY
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHH
LKATLGVSDAQKSAWYAHWIGLGFASLERRLAAEPRVGRCVFGDTPGLADLCLVPQVWNA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCCC
KRFAVPLDAYPTLMRLYGHAAALPAFARAEPSLQPDAEPPPA
CEEEEECCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEATLYGYFRSSASYRVRIALNLKGIDYRQIPVHLLRGGGEQLGEAYRQIQPDGVVPAF
CCCEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEE
VDGDGPPLAQSLAIVEYLDECFPQPPLLPSAPADRAYVRAVALQIACEIHPLNNLRVLSY
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHH
LKATLGVSDAQKSAWYAHWIGLGFASLERRLAAEPRVGRCVFGDTPGLADLCLVPQVWNA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCCC
KRFAVPLDAYPTLMRLYGHAAALPAFARAEPSLQPDAEPPPA
CEEEEECCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]