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The map label for this gene is proP [H]

Identifier: 238025157

GI number: 238025157

Start: 2279344

End: 2280690

Strand: Reverse

Name: proP [H]

Synonym: bglu_2g18230

Alternate gene names: 238025157

Gene position: 2280690-2279344 (Counterclockwise)

Preceding gene: 238025159

Following gene: 238025156

Centisome position: 80.67

GC content: 71.71

Gene sequence:

>1347_bases
ATGGCGATCGGCAACGCAATGGAGTGGTTCGATTTCGGCGTCTACAGCTATATCGCCGTGACGCTCGGCGAAGTGTTCTT
TCCGTCCACCAGCGGCCCGGTGCAGTTGATCGCCACTTTCGGCACGTTCGCGGCCGCGTTCCTCGTGCGGCCGATCGGCG
GCCTGGTGTTCGGCCCGCTCGGCGACCGCATCGGCCGCCAGCGCGTGCTCGCGATGACGATGATCCTGATGGCGCTCGGC
ACCTTCGCGATCGGCCTGATCCCCGGCTACGGCTCGATCGGCATCCTCGCGCCCTTGCTGCTGCTGGTCGCGCGGCTCGT
GCAGGGCTTCTCGACGGGCGGCGAGTACGGCGGCGCGGCCACCTTCATCGCCGAGTTCGCGGCGGACCGGCATCGCGGCT
TGATGGGCAGCTTCCTCGAATTCGGCACGCTGGCCGGCTACCTGCTCGGCGCGGGCACCGTCACGGCGCTCGCGGCGGCG
CTCTCGCCCGCGCAGATGCTCGACTGGGGCTGGCGCGTGCCGTTCTTCATCGCCGGCCCGCTCGGGCTGATCGGCCTCTA
CATCCGCACCCGCCTCGAGGAAACGCCGGCGTTCCGCAAGGAGGCCGAGCGCCGCGAGCGGTCCGGGCGCCCGCACGCGC
GTCCGTCGTTGGCCGCGCTGCTGGCCGGGCAGTGGCGGCCAATGCTGGTCTGCGTCGGGCTGGTACTCATCTTCAACGTG
ACCGACTACATGGTGCTCGCCTACCTGCCGAACTACCTGTCGGCCACGCTGCACATGGCGCAGACCCGCAGCCTGGCGAT
GGTGTCGCTGGTGATGCTGGCGATGATGCCGCTCACGCTCGCGGCCGGCTGGCTGTCGGACCGGGTGGGCCGGCGCCCGG
TGCTGCTGGCCGGCTGCCTCGGGCTGATCGTGCTGGCGGTGCCGGCGCTGCTGCTGATTCGCGGCGGCCAAGGCGTGCCG
CTGTTCGCCGGCATGATGGTGCTGGGCGTGCTGCTCTGCTGCTTTTGCGGCGTGATGCCTTCGGCGTTGCCGGCGCTGTT
TCCCACCTCGGTGCGCTATGGCGCGCTGGCGATCGGCTTCAACGTGTCGGTGTCGCTGTTCGGCGGCACCACGCCGCTCG
TCACGGCATGGCTGGTCAGCGCCACCGGCAACCTGATGATGCCGGCCTACTACCTGATGGGGGCGGCGGCGCTCGGCGCG
GTGGCGGTGCTGGCGGCGCGCGAGAGCGCGGGCCGGCCGCTGCCGGGCTCGGCGCCATGCGTGGCGACCCAGGCCGAGGC
GCAGCGCCTGCGCCGTGACGGCGCGGGCCGGCTCGCCCAGGCCGGCTTCACCCCCGCCAGGAAGTAG

Upstream 100 bases:

>100_bases
GCCCCGCTCGCCGGGCGCGCCTGAAGCGCGCGCGGCCGCCAGCGACCAGCACGAGGTGACGGTCGTCGATCGCGCGCTGC
TGCGGCGCGCAGTGGGCGCG

Downstream 100 bases:

>100_bases
CCGCCGCCATCGCCGGCAAGCCCCGCCGGCCGGACCGTTCCCGGTTCCGGCACCGGCCGGCTTTGCGCGGCGTGCGGAAC
CGGTTGCGTGCGCAACGGCT

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAIGNAMEWFDFGVYSYIAVTLGEVFFPSTSGPVQLIATFGTFAAAFLVRPIGGLVFGPLGDRIGRQRVLAMTMILMALG
TFAIGLIPGYGSIGILAPLLLLVARLVQGFSTGGEYGGAATFIAEFAADRHRGLMGSFLEFGTLAGYLLGAGTVTALAAA
LSPAQMLDWGWRVPFFIAGPLGLIGLYIRTRLEETPAFRKEAERRERSGRPHARPSLAALLAGQWRPMLVCVGLVLIFNV
TDYMVLAYLPNYLSATLHMAQTRSLAMVSLVMLAMMPLTLAAGWLSDRVGRRPVLLAGCLGLIVLAVPALLLIRGGQGVP
LFAGMMVLGVLLCCFCGVMPSALPALFPTSVRYGALAIGFNVSVSLFGGTTPLVTAWLVSATGNLMMPAYYLMGAAALGA
VAVLAARESAGRPLPGSAPCVATQAEAQRLRRDGAGRLAQAGFTPARK

Sequences:

>Translated_448_residues
MAIGNAMEWFDFGVYSYIAVTLGEVFFPSTSGPVQLIATFGTFAAAFLVRPIGGLVFGPLGDRIGRQRVLAMTMILMALG
TFAIGLIPGYGSIGILAPLLLLVARLVQGFSTGGEYGGAATFIAEFAADRHRGLMGSFLEFGTLAGYLLGAGTVTALAAA
LSPAQMLDWGWRVPFFIAGPLGLIGLYIRTRLEETPAFRKEAERRERSGRPHARPSLAALLAGQWRPMLVCVGLVLIFNV
TDYMVLAYLPNYLSATLHMAQTRSLAMVSLVMLAMMPLTLAAGWLSDRVGRRPVLLAGCLGLIVLAVPALLLIRGGQGVP
LFAGMMVLGVLLCCFCGVMPSALPALFPTSVRYGALAIGFNVSVSLFGGTTPLVTAWLVSATGNLMMPAYYLMGAAALGA
VAVLAARESAGRPLPGSAPCVATQAEAQRLRRDGAGRLAQAGFTPARK
>Mature_447_residues
AIGNAMEWFDFGVYSYIAVTLGEVFFPSTSGPVQLIATFGTFAAAFLVRPIGGLVFGPLGDRIGRQRVLAMTMILMALGT
FAIGLIPGYGSIGILAPLLLLVARLVQGFSTGGEYGGAATFIAEFAADRHRGLMGSFLEFGTLAGYLLGAGTVTALAAAL
SPAQMLDWGWRVPFFIAGPLGLIGLYIRTRLEETPAFRKEAERRERSGRPHARPSLAALLAGQWRPMLVCVGLVLIFNVT
DYMVLAYLPNYLSATLHMAQTRSLAMVSLVMLAMMPLTLAAGWLSDRVGRRPVLLAGCLGLIVLAVPALLLIRGGQGVPL
FAGMMVLGVLLCCFCGVMPSALPALFPTSVRYGALAIGFNVSVSLFGGTTPLVTAWLVSATGNLMMPAYYLMGAAALGAV
AVLAARESAGRPLPGSAPCVATQAEAQRLRRDGAGRLAQAGFTPARK

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=443, Percent_Identity=48.9841986455982, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1788292, Length=414, Percent_Identity=32.3671497584541, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789941, Length=418, Percent_Identity=35.1674641148325, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788942, Length=377, Percent_Identity=32.6259946949602, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87082231, Length=224, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082404, Length=317, Percent_Identity=24.6056782334385, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47080; Mature: 46949

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2 ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIGNAMEWFDFGVYSYIAVTLGEVFFPSTSGPVQLIATFGTFAAAFLVRPIGGLVFGPL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCH
GDRIGRQRVLAMTMILMALGTFAIGLIPGYGSIGILAPLLLLVARLVQGFSTGGEYGGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TFIAEFAADRHRGLMGSFLEFGTLAGYLLGAGTVTALAAALSPAQMLDWGWRVPFFIAGP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEECH
LGLIGLYIRTRLEETPAFRKEAERRERSGRPHARPSLAALLAGQWRPMLVCVGLVLIFNV
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
TDYMVLAYLPNYLSATLHMAQTRSLAMVSLVMLAMMPLTLAAGWLSDRVGRRPVLLAGCL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GLIVLAVPALLLIRGGQGVPLFAGMMVLGVLLCCFCGVMPSALPALFPTSVRYGALAIGF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
NVSVSLFGGTTPLVTAWLVSATGNLMMPAYYLMGAAALGAVAVLAARESAGRPLPGSAPC
EEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VATQAEAQRLRRDGAGRLAQAGFTPARK
EECHHHHHHHHHCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
AIGNAMEWFDFGVYSYIAVTLGEVFFPSTSGPVQLIATFGTFAAAFLVRPIGGLVFGPL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCH
GDRIGRQRVLAMTMILMALGTFAIGLIPGYGSIGILAPLLLLVARLVQGFSTGGEYGGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TFIAEFAADRHRGLMGSFLEFGTLAGYLLGAGTVTALAAALSPAQMLDWGWRVPFFIAGP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEECH
LGLIGLYIRTRLEETPAFRKEAERRERSGRPHARPSLAALLAGQWRPMLVCVGLVLIFNV
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
TDYMVLAYLPNYLSATLHMAQTRSLAMVSLVMLAMMPLTLAAGWLSDRVGRRPVLLAGCL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GLIVLAVPALLLIRGGQGVPLFAGMMVLGVLLCCFCGVMPSALPALFPTSVRYGALAIGF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
NVSVSLFGGTTPLVTAWLVSATGNLMMPAYYLMGAAALGAVAVLAARESAGRPLPGSAPC
EEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VATQAEAQRLRRDGAGRLAQAGFTPARK
EECHHHHHHHHHCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]