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The map label for this gene is yueD [H]

Identifier: 238025159

GI number: 238025159

Start: 2282891

End: 2283649

Strand: Reverse

Name: yueD [H]

Synonym: bglu_2g18250

Alternate gene names: 238025159

Gene position: 2283649-2282891 (Counterclockwise)

Preceding gene: 238025161

Following gene: 238025157

Centisome position: 80.77

GC content: 74.44

Gene sequence:

>759_bases
ATGACCCCGACCCATTCCACGCCGCCCGCGATCCGCGCGATCGTCACCGGCCACACGCGCGGCCTCGGCGCCGCGCTCGC
CACGCAGCTGCTGCGCCGCGACATCGCCGTGCTCGGCGTGTCGCGCGGCGCGCACCCGTCGCTCGCCGCCCAATACGGCG
GCCGCTTCGCCGAGGCCGCGCTCGACCTCTCCGACCCCGCGCGGGTGGCCGCGTGGCTGGCCGGCGGCGAGCTCGAACGC
TTCGCGTCCGGCGCGCGCACGGTGCTGCTGATCAACAACGCGGGCACGGTCGAGCCGATCGGCCCGCTCGCGGTGCAGAC
GCCCGAGGCGATCGCGCGCGCGGTGGCGCTGAACGTGACGGCGCCGCTGCTGTTCGCGAGCGCGGTCGCCCAGCTCGGCG
ACGGCTCGCTCGAGCGGCGCATCGCGCACGTCTCGAGCGGCGCCGCGCGCAACGCCTATGCGGGCTGGAGCGTCTATTGC
GCGACCAAGGCCGCGCTCGATCATCACGCGCGCGCGGTCGCGCTCGATCACAACGGTCCGCTGCGGATCGCCAGCGTCGC
GCCGGGCGTGGTCGACACCGACATGCAGGCCACCATCCGCGCCACCGACGCGAGCCGCTTTCCGCAGCTCGACCGGTTCG
AACGGCTGAAGGCGAGCGGCGCGCTGACCACGCCCGACGATGCGGCGCGCAAGCTGATCGACCACCTGCTCGACGACAGT
TTCGGCAACCCGCCGATCAGCGACGTGCGCGACTGGTGA

Upstream 100 bases:

>100_bases
CGCGCCGGTCCGCATCGCCGGGCGGCGGCCCGCGGGCCGGCCCGCCTGCCGGCGCGCCGCCGCTCACGATCGCCTCCCCG
TCACCTCACGCCACTCCGAC

Downstream 100 bases:

>100_bases
GCAAGGGCTGCGGCGCGAGCCCGCTCGTAGCGGCGCTCGGCTACGCGCCGCCGGCCGCGAGCCGGCATCGCTCGCGCATC
GCGGGCCGGCAGGCCGCTCG

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTPTHSTPPAIRAIVTGHTRGLGAALATQLLRRDIAVLGVSRGAHPSLAAQYGGRFAEAALDLSDPARVAAWLAGGELER
FASGARTVLLINNAGTVEPIGPLAVQTPEAIARAVALNVTAPLLFASAVAQLGDGSLERRIAHVSSGAARNAYAGWSVYC
ATKAALDHHARAVALDHNGPLRIASVAPGVVDTDMQATIRATDASRFPQLDRFERLKASGALTTPDDAARKLIDHLLDDS
FGNPPISDVRDW

Sequences:

>Translated_252_residues
MTPTHSTPPAIRAIVTGHTRGLGAALATQLLRRDIAVLGVSRGAHPSLAAQYGGRFAEAALDLSDPARVAAWLAGGELER
FASGARTVLLINNAGTVEPIGPLAVQTPEAIARAVALNVTAPLLFASAVAQLGDGSLERRIAHVSSGAARNAYAGWSVYC
ATKAALDHHARAVALDHNGPLRIASVAPGVVDTDMQATIRATDASRFPQLDRFERLKASGALTTPDDAARKLIDHLLDDS
FGNPPISDVRDW
>Mature_251_residues
TPTHSTPPAIRAIVTGHTRGLGAALATQLLRRDIAVLGVSRGAHPSLAAQYGGRFAEAALDLSDPARVAAWLAGGELERF
ASGARTVLLINNAGTVEPIGPLAVQTPEAIARAVALNVTAPLLFASAVAQLGDGSLERRIAHVSSGAARNAYAGWSVYCA
TKAALDHHARAVALDHNGPLRIASVAPGVVDTDMQATIRATDASRFPQLDRFERLKASGALTTPDDAARKLIDHLLDDSF
GNPPISDVRDW

Specific function: Reduces benzil stereospecifically to (S)-benzoin. Can also reduces 1-phenyl-1,2-propanedione, 1,4-naphthoquinone, 1-(4- methyl-phenyl)-2-phenyl-ethane-1,2-dione, 1-(4-fluoro-phenyl)-2- phenyl-ethane-1,2-dione, methyl benzoylformate, p- nitrobenzaldehyde i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4507185, Length=174, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322227, Length=229, Percent_Identity=31.4410480349345, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322226, Length=262, Percent_Identity=28.6259541984733, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 26309; Mature: 26178

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTHSTPPAIRAIVTGHTRGLGAALATQLLRRDIAVLGVSRGAHPSLAAQYGGRFAEAA
CCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCHHHHHH
LDLSDPARVAAWLAGGELERFASGARTVLLINNAGTVEPIGPLAVQTPEAIARAVALNVT
HCCCCHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHH
APLLFASAVAQLGDGSLERRIAHVSSGAARNAYAGWSVYCATKAALDHHARAVALDHNGP
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCEEEEEECCCC
LRIASVAPGVVDTDMQATIRATDASRFPQLDRFERLKASGALTTPDDAARKLIDHLLDDS
EEEEECCCCCCCCCCHHEEEECCHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
FGNPPISDVRDW
CCCCCCHHCCCC
>Mature Secondary Structure 
TPTHSTPPAIRAIVTGHTRGLGAALATQLLRRDIAVLGVSRGAHPSLAAQYGGRFAEAA
CCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCHHHHHH
LDLSDPARVAAWLAGGELERFASGARTVLLINNAGTVEPIGPLAVQTPEAIARAVALNVT
HCCCCHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHH
APLLFASAVAQLGDGSLERRIAHVSSGAARNAYAGWSVYCATKAALDHHARAVALDHNGP
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCEEEEEECCCC
LRIASVAPGVVDTDMQATIRATDASRFPQLDRFERLKASGALTTPDDAARKLIDHLLDDS
EEEEECCCCCCCCCCHHEEEECCHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
FGNPPISDVRDW
CCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11745140; 11796169 [H]