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The map label for this gene is vanB [H]

Identifier: 238024816

GI number: 238024816

Start: 1853413

End: 1854378

Strand: Reverse

Name: vanB [H]

Synonym: bglu_2g14540

Alternate gene names: 238024816

Gene position: 1854378-1853413 (Counterclockwise)

Preceding gene: 238024817

Following gene: 238024814

Centisome position: 65.59

GC content: 65.63

Gene sequence:

>966_bases
GTGAGCGAGGCGACGTTGATCGTCGAGGTCGTGCGCAAATGGGACGAGGCACGCGGCATCTGCGGTTTCGAACTGAGGCG
CCCGGACCGTGCGCCGCTGCCCGGCTTCAGCGCAGGTGCGCATATCGACGTCCATCTGCCCGGCGGAATCGTGCGTCAAT
ATTCGCTATGCAGCAGCCCGGCACGCAACGACCGCTATGAAATTGCGGTGCTGCGTGACGAGCGCGGTCGCGGAGGATCG
ATGGCGATCCACGACCACGTGCACGAAGGCGACCAGATCCGAATCGGTGCTCCCCGCAACCATTTTCCGCTCGAACTTCG
CGCGGCCCGGCATCTGCTGCTGGCCGGGGGGATCGGCGTCACGCCGCTCCTGTCGATGGCCGAGCATCTGCACGCGCTCG
GGGCGTCGTTCGAGATGCACTACTGTACGCGCTCACGTGATCGGACCGCGTTCGCGCAACGGCTGGCCGGATCGGCGTAT
CACGATCATGTGCAGATCCATTTCGATGACGCGGGCGAGCATCAGGCGTTCGAACTCGACCAGGTGCTCGCGGCAGCGGC
AGCCGATACCCATCTCTATGTTTGCGGTCCGCGCGGCTTCATGGAGGCGGCGCTCGCCGCGGCGCGTGCGCATGGCTGGC
CAGACGAGCGGCTGCATTACGAGTTTTTTTCCGGTGCGGTGGCGGATTCGGCCGAGGATGGAGCGTTCCAGGTCCGGGTG
GCGAGCAGCGGGGCCGTGATCGACGTGCCGGCGGGATGCTCGATCGTCGATGCGCTCGCAAAGCACGGCGTCGAAGTGCT
GACCTCGTGCGAGCAGGGCGTTTGCGGCACGTGCATGACGCGTGTGCTCGAAGGGCAGCCCGATCATCGGGACAGCTATC
TGACCGATGAGGAGAAGGCGGCGGGCGAGTACCTCATGCCGTGCTGCTCACGAGCGAAATCCGCCCTGCTGGTGCTCGAT
CTGTAG

Upstream 100 bases:

>100_bases
GCGTTCTCTCCCGCAAGGTCATCGACCGCCTGCTGGCAGAGGAGCGTGCATTGCCCGCCGGACGTCCGGTGGTGCCGATC
GTGCCGACCAGGGAGGCATC

Downstream 100 bases:

>100_bases
ATTCTTGCCGTCCCGGGCTGCAACGGCGCCCACCGCGCCGTGCCGACGCCCGGGAGCGCGCCGGGTTGTGTCGCGTCGTG
AGGAATTAGCTCGAGACCGG

Product: ferredoxin

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSEATLIVEVVRKWDEARGICGFELRRPDRAPLPGFSAGAHIDVHLPGGIVRQYSLCSSPARNDRYEIAVLRDERGRGGS
MAIHDHVHEGDQIRIGAPRNHFPLELRAARHLLLAGGIGVTPLLSMAEHLHALGASFEMHYCTRSRDRTAFAQRLAGSAY
HDHVQIHFDDAGEHQAFELDQVLAAAAADTHLYVCGPRGFMEAALAAARAHGWPDERLHYEFFSGAVADSAEDGAFQVRV
ASSGAVIDVPAGCSIVDALAKHGVEVLTSCEQGVCGTCMTRVLEGQPDHRDSYLTDEEKAAGEYLMPCCSRAKSALLVLD
L

Sequences:

>Translated_321_residues
MSEATLIVEVVRKWDEARGICGFELRRPDRAPLPGFSAGAHIDVHLPGGIVRQYSLCSSPARNDRYEIAVLRDERGRGGS
MAIHDHVHEGDQIRIGAPRNHFPLELRAARHLLLAGGIGVTPLLSMAEHLHALGASFEMHYCTRSRDRTAFAQRLAGSAY
HDHVQIHFDDAGEHQAFELDQVLAAAAADTHLYVCGPRGFMEAALAAARAHGWPDERLHYEFFSGAVADSAEDGAFQVRV
ASSGAVIDVPAGCSIVDALAKHGVEVLTSCEQGVCGTCMTRVLEGQPDHRDSYLTDEEKAAGEYLMPCCSRAKSALLVLD
L
>Mature_320_residues
SEATLIVEVVRKWDEARGICGFELRRPDRAPLPGFSAGAHIDVHLPGGIVRQYSLCSSPARNDRYEIAVLRDERGRGGSM
AIHDHVHEGDQIRIGAPRNHFPLELRAARHLLLAGGIGVTPLLSMAEHLHALGASFEMHYCTRSRDRTAFAQRLAGSAYH
DHVQIHFDDAGEHQAFELDQVLAAAAADTHLYVCGPRGFMEAALAAARAHGWPDERLHYEFFSGAVADSAEDGAFQVRVA
SSGAVIDVPAGCSIVDALAKHGVEVLTSCEQGVCGTCMTRVLEGQPDHRDSYLTDEEKAAGEYLMPCCSRAKSALLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=303, Percent_Identity=38.9438943894389, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787658, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787098, Length=279, Percent_Identity=26.5232974910394, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788903, Length=246, Percent_Identity=28.0487804878049, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34812; Mature: 34680

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEATLIVEVVRKWDEARGICGFELRRPDRAPLPGFSAGAHIDVHLPGGIVRQYSLCSSP
CCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCC
ARNDRYEIAVLRDERGRGGSMAIHDHVHEGDQIRIGAPRNHFPLELRAARHLLLAGGIGV
CCCCCEEEEEEECCCCCCCCEEEECCCCCCCEEEECCCCCCCCEEHHHHHHEEEECCCCH
TPLLSMAEHLHALGASFEMHYCTRSRDRTAFAQRLAGSAYHDHVQIHFDDAGEHQAFELD
HHHHHHHHHHHHHCCCEEEEEHHCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHH
QVLAAAAADTHLYVCGPRGFMEAALAAARAHGWPDERLHYEFFSGAVADSAEDGAFQVRV
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCCEEEEEE
ASSGAVIDVPAGCSIVDALAKHGVEVLTSCEQGVCGTCMTRVLEGQPDHRDSYLTDEEKA
ECCCCEEECCCCCHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
AGEYLMPCCSRAKSALLVLDL
CCHHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure 
SEATLIVEVVRKWDEARGICGFELRRPDRAPLPGFSAGAHIDVHLPGGIVRQYSLCSSP
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCC
ARNDRYEIAVLRDERGRGGSMAIHDHVHEGDQIRIGAPRNHFPLELRAARHLLLAGGIGV
CCCCCEEEEEEECCCCCCCCEEEECCCCCCCEEEECCCCCCCCEEHHHHHHEEEECCCCH
TPLLSMAEHLHALGASFEMHYCTRSRDRTAFAQRLAGSAYHDHVQIHFDDAGEHQAFELD
HHHHHHHHHHHHHCCCEEEEEHHCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHH
QVLAAAAADTHLYVCGPRGFMEAALAAARAHGWPDERLHYEFFSGAVADSAEDGAFQVRV
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCCEEEEEE
ASSGAVIDVPAGCSIVDALAKHGVEVLTSCEQGVCGTCMTRVLEGQPDHRDSYLTDEEKA
ECCCCEEECCCCCHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
AGEYLMPCCSRAKSALLVLDL
CCHHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579070 [H]